FastQCFastQC Report
Thu 26 May 2016
SRR2099103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212043
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACAGTGAAGCGAAATGACACAAAATAGAACCTGCAAAT22851.0776116165117453No Hit
TGCTACAGCTGCGTCCGACGATCGGCTTGCTACAGTTTTTTT20450.9644270265936626No Hit
TGCTGCATCATGAAGAAATCCTCGGGCTTGAGGAAGAGGTCT15290.7210801582697849No Hit
TGCTACAGGGTGGCAACATATACCAGCTTCCCCACTGGGTGT10970.5173478964172361No Hit
TGCTACAGCTGCGGTCCGACGATCGGCTTGCTACAGTTTTTT9890.4664148309540989No Hit
TGCTACAGATAAGTCCGACGATCATCCTGCTACAGTTTTTTT7620.35936107298991243No Hit
TGCTACAGCTACGTCTACTTGCTACAGTTTTTTTTTTTTTTT6200.2923935239550468No Hit
TGCTACAGGTCATTCTACAGTCCGACGATCCGTCTGCTACAG5460.25749494206363804No Hit
TGCTACAGTTGTCAGTCCGACGATCAAAAACTGCTAGTTTTT5180.24429007323986174No Hit
TGCTACAGAGCTGTCCACTGTAGGAGGTGGAATATGGCAAGA5120.24146045849190967No Hit
TGCTACAGGATGCCTAGGTTTTGCATATGACCTGCAGCCTAA5050.23815924128596558No Hit
TTCGACAGGACACCCAATCCGGCGGCCAGGACCGCAAACGCA5040.23768763882797356No Hit
TGCTACTGATGGGTTATTCGGCAGTTGTGCCAATGAGTTCAA4880.23014199950010136No Hit
CGCTGCAGCCGAGTCGCGTGCGAGGTCTGCGGGGGAGCGCGC4870.2296703970421094No Hit
TGCTACAGGTGAGATCTGCGTGCTACAGTTTTTTTTTTTTTT4750.22401116754620526No Hit
TGCTACAGACCCGGCAGCTTTGGATCGCCGAAATCCTGGTCT4710.2221247577142372No Hit
TTCGACAGCGCAATTATCAGCTTAGCCCTGACCAGAATCGGA4690.22118155279825322No Hit
TGCTACAGATCCGCGTGGTGGACGTCCAAACCGTGGCAGCAG4690.22118155279825322No Hit
TGCTTCAGTTCTGTCCACATGACCTCCTCGAAACCGTGGCGC4670.22023834788226915No Hit
TGCTACAGCTTTGTTAGAAGAATATTGTGAAAATGACCACAT4620.2178803355923091No Hit
TGCTACAGGATGGGTTGCGGTGAGCCGAGATCGTGCCATTGC4560.21505072084435703No Hit
TGCTTCAGTTCTTCCACATGACCTCCTCGAAACCGTGGCGCA3930.18533976599086033No Hit
TGCTACAGTCAGAGGAGCAGTGGAGCCAGCTCCCTCACCCCT3850.18156694632692427No Hit
TGCTACAGACTACCTGAGTAGCTGGGACTACAGGCGCCTGCC3720.1754361143730281No Hit
TGCAGCAGCAGCAAGAGCGCGGCTTTCGCGGCCGGCGCCAAC3710.1749645119150361No Hit
TGCTACAGGTCATCCGACGATCATCGGACTGTAGAACTCTGA3680.17354970454106008Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGCTACAGATAGTGATCGACTGGTGATAAAGACGATAATAGC3540.1669472701291719No Hit
TACTACAGGTGGGCCGGGCCTCGCCCGGCGCATGGGCCCGCA3390.15987323325929176No Hit
TGCTACAGGCTTGTGCACGCAGGTGCGAGTGGAATTCATGGA3260.1537424013053956No Hit
TGCTACAGATAGGGCCAATGGGTTATCTGGACCAAAGGTGGG3220.15185599147342757No Hit
TGCTGCAGCAGGTGAAAATGCTCCCTTCCCATGGGAAAGAGC3050.14383874968756338No Hit
TGCTACAGTCTTGCCGAAGTGGGAGGATCACTTGAGCTCAGG2990.14100913493961129No Hit
TGCTACAGCCGCGTGAGCTGTAGCCTGAGGGACGTGAGGGAC2920.13770791773366722No Hit
TCCTAGAGAGCTGGGTAGGCCTCTGTAAAATCGATCGAGAGG2790.1315770857797711No Hit
TGCTACAGGAGTGCGTGGCGAGCGTGCCCTGGCTACCGCACT2770.13063388086378705No Hit
TGCTACAGAAACTGCAGCCATTACACCCATATCGGTACTTGA2750.12969067594780304No Hit
TGCTGCTGCCGCCGCCTCAGCGTCCCAAGTACCTGGGACCAC2750.12969067594780304No Hit
TGCCACAAACGACAGGAGCGCTAGGCCAATGGCCCTGAACCA2710.12780426611583498No Hit
TGCGACAGTCCCTCAAGCGCAGCAAAACACGGCTGGCAACGC2620.1235598439939069No Hit
TGCTGCATGAGTGGTTCCATGCTCGTGCCCGTGCCCACTCGA2620.1235598439939069No Hit
TGCTACAGCTACTCTACTTGCTACAGTTTTTTTTTTTTTTTT2610.12308824153591487No Hit
TGCTACAGGGTTGGGAATTTCAAAGGAAATTTATGGGAACAA2600.12261663907792288No Hit
TGCTACAGCAGTAGGTCTGACATGCACACATATGTTTATTGC2510.11837221695599479No Hit
TGCTGCATGAGTGTTCCATGCTCGTGCCCGTGCCCACTCGAT2470.11648580712402673No Hit
TGCTACAGGGCGGTGACCGACAGATAACCACAATGAAACTAG2470.11648580712402673No Hit
TGCTACAGGTAAATCCGCCTGCTACAGTTTTTTTTTTTTTTT2420.11412779483406668No Hit
TGCTGCATCATGCGAAGAAATCCTCGGGCTTGAGGAAGAGGT2390.11271298746009063No Hit
CGCTGCAGCCGAGGTCGCGTGCGAGGTCTGCGGGGGAGCGCG2360.11129818008611461No Hit
TGCTACAAGGCGGGGGAAAAAAAGATTGTTTCTTTTCAATAT2350.11082657762812259No Hit
TGCTACAGCTCCGTCAGGAGAGGTTGATTCGATCTATATAAC2330.10988337271213856No Hit
TGCTACAGCTGCCGTCCGACGATCGGCTTGCTACAGTTTTTT2320.10941177025414657No Hit
TGCAACACCTATAAGCAATACATTTTCTAAAATAGAACGCAG2310.10894016779615455No Hit
TGTTACAGGTCAAATCGAATTCATCGACTTCCCGCACCCGCA2280.10752536042217851No Hit
TGCTACAGCCACGTTTGATCTGGCTGGAAGCTGTAGATGGAG2250.1061105530482025No Hit
TGCTATGGAGCTAAAATGACGAAATCACTAAAATACGTGAAA2240.10563895059021047No Hit
TGCTACTGGAACGTGCGGGTTAGGTCATCGTCCATGGTGGTG2230.10516734813221848No Hit
TGCTACAGTCAACGAGAACCGGATTGTCGAGGTCGACAACGG2220.10469574567422645No Hit
TACTACAAAGCTTTGACAGCCAATGGCCGGAATGGGAGCGGG2220.10469574567422645No Hit
TGCAACACCGCATCCCAGATATGGGCTTGCCAGGGCAGGGTG2200.10375254075824243No Hit
TGCTACAGGGCAAATAACTTTTCTAACAATTAGAAAAACTAC2180.1028093358422584No Hit
TGCTACAGCTCCGTGCCAGGCATCCTGGGAACCCCTTCATGG2140.10092292601029036No Hit
TGCTACAGTCTGCAGTCCGACGATCGGGCTGTAGAACTCTGA2130.10045132355229835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAG453.3796823E-936.0188837
AGACTAA502.382876E-1036.0188837
AGGTACG404.8245056E-836.0188837
CTCGTTT453.3796823E-936.0188839
CCCTCAA251.4409538E-436.01888310
TAAAGTA251.4409538E-436.0188837
ATAGGGC453.3796823E-936.0188839
CTATCGT200.00209783436.01888311
AGCCCGG200.00209783436.0188837
GGCACGG251.4409538E-436.0188838
CGCATGC200.00209783436.0188837
ATTACGC200.00209783436.0188839
GGTCCGC404.8245056E-836.0188839
GCGCCTA453.3796823E-936.0188838
GTCTATC200.00209783436.0188839
GGTTTAG251.4409538E-436.0188838
AGGGTGT200.00209783436.0188837
TTCCGAA251.4409538E-436.0188839
AGGCGAT200.00209783436.0188837
AGGCGAC251.4409538E-436.0188837
Produced by FastQC (version 0.11.5)