FastQCFastQC Report
Thu 26 May 2016
SRR2099102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692188
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGCATGCGCCTTGGGTGGGAGGAGGAGGAGGTGGAAGAGC15000.2167041324033355No Hit
ACGTGATTGCCTCGTCGCGTCGCATATCCGTCCGGAAGTTCCG14150.2044242315671465No Hit
GCATGCATGCCTTGAAGAGGCGGTCTATGCCCCAAAGGCCTGG13960.20167931255670427No Hit
ACGAGCTTCAACTAATAACAATACAGGACTCTTTCGAGGCCCT11960.1727854282362595No Hit
ACGTGCATTCCGGGGAACCTTTGGGAGCCGTGACTCCCTGTGC10920.15776060838962824No Hit
ACGTGCATTCGAAAGACTCTGTAGCTTTCTGTCCAGAAAGTAC10650.15385993400636822No Hit
ACGTGCATACTGGCGCGATCCCTTTACGTGCATTTTTTTTTTT10180.1470698711910637No Hit
AAGTGCAGAGCGTGTTCTACTTGATGTGCTTGCTTTGCATGCC9030.13045588770680797No Hit
AGGTGCAAAGCTTCAGCAGGTTTTCAAAACTCGCCTGGCTAAG8990.1298780100203991No Hit
ACGTGCATTAGTTGGCGTCCACCGCCCCAGGAGGCCGCCTGAG8540.12337688604829901No Hit
ACGTGCATACCCCGCTGGACGAGGCGGAGTACCAGGAGATCCG8330.12034302819465231No Hit
ACGTGCATACTGGGCGATCCCTTTACGTGCATTTTTTTTTTTT7950.11485319017376781No Hit
ACGTACATGCTTTCTTCACGTGCATTTTTTTTTTTTTTTTTTT7500.10835206620166775No Hit
ACGTGCATAACTATGGAAGTGCATCACTCCAAGTGCGGCGCAC7450.10762971909365664No Hit
ACCTGCATGCGCCGTTGGGTGGGAGGAGGAGGAGGTGGAAGAG7430.1073407802504522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG200.001836561737.0199513
CGTAGTG200.001839171337.00924711
ACCCGTT200.001839171337.00924710
TTCGCAT200.001839171337.00924711
AATCCGT355.6191493E-737.0065779
GAGCGTA501.7826096E-1037.0065778
TTGCGTA501.7826096E-1037.0065778
TTACGTC551.2732926E-1137.0065778
TAGGCGC251.2318288E-437.0065778
CAAGCGG200.001839824137.00657317
ACATCGC200.001839824137.0065739
ATAGGCG403.8307917E-836.9985547
ATAGCGT452.6175258E-936.9985547
GCATATA6850.036.9985545
GCATACT12450.036.8499685
TGCATAC34350.036.780484
TGCATAT30400.036.7524874
GCATAAG7500.036.7518965
CGAGCTT11850.036.683682
TGCATAA30450.036.6313934
Produced by FastQC (version 0.11.5)