FastQCFastQC Report
Thu 26 May 2016
SRR2099101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553681
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATGCATAAGGGCCAAGGGTCAGAGGCTCTGCCAACCGAA24450.4415900130219386No Hit
CTGATGCAGCGAGTCCATGATCCGCAGCGCGTGGGCCCGGAC16890.30504929733908154No Hit
CTGATGCAGATGATCGGTGCTGATGCATTTTTTTTTTTTTTT15470.2794027607954761No Hit
CTGATGCGCTATGCCGAGCTTGCGACGCAAGTTGCCGATGTG12320.22251079592761897No Hit
CTGATGCTAGGGGCCGACCTGCTCCAGCGTCGCCCTGACCTG12230.22088531121710878No Hit
CTGATCCCGGCTACAGAGATGTGATGCTCAGATTGGTCAGGC11770.21257727825227885No Hit
CTGATGCAAATGCGGAGTCCGCGGCCAGCACAGCCTCTAGCT9090.16417395576153057No Hit
CTGATGCACGCTGACAGGGCCAGAGGGGAAGAGCGCAGAATA8180.1477384992441496No Hit
CTGGCGCACTGTGGCGTGCCGAATGGCTACGGCCAGTCGATG8170.14755788983187068No Hit
CTGATGCATGATTGCAAGGGTTTCTTTGGTGGGTGCAAGGTT8130.14683545218275507No Hit
CTGATGCAATACATTTAGGGGGGCAGAGCTCAGACACCAATT6430.1161318520953401No Hit
CTGATGCATCCTAAGAAAGGCCCAGAGCATTCCAGCCAGGAA5750.10385041206037411No Hit
CTGATGCAGCGACTGGACTTCTGATGTCCTGAGGTGGAAGTC5570.1005994426393537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC200.00210305636.0108412
GAATCGG251.4458563E-436.0108411
CGGATAG200.00210305636.0108411
TACGACG200.00210305636.0108411
TAAGGTT251.4458563E-436.0108411
TTATAGG502.401066E-1036.007599
TATCGCA502.401066E-1036.007599
GACTCCG251.446625E-436.0075910
CTTCGCG200.00210398936.0075879
CTATCGC356.9585803E-736.0075879
CCGTCCA404.8621587E-836.0075879
CCGAGTA200.00210398936.00758710
TCATCCG404.8621587E-836.00758710
GGTGACG200.00210398936.00758713
TAGGCGA200.00210398936.0075879
TCGCGAG200.00210398936.0075879
GGCTAGT200.00210398936.0075879
ACTATGG251.4473939E-436.0043378
CAAACGG650.036.0043377
CAACGCG502.401066E-1036.0043377
Produced by FastQC (version 0.11.5)