FastQCFastQC Report
Thu 26 May 2016
SRR2099100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504405
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGCATAAGGGCAGCATCATGTTAACTGATCAGCTGGTGA42730.847136725448796No Hit
AGTCTGCAGTTTGGCTGCTGGGGTGCGCCTGGCTGGTCGGCCT21900.4341749189639278No Hit
AGTCTGCACGATATCCGACGATCGTCAAAGTCTGCATTTTTTT20980.4159356072996897No Hit
AGTCTGCAGATTACACGCCGTCTTTCATCGGCCGGATCGCCTG19070.3780692102576303No Hit
AGTCTGCAGGGAGGTCCGCGATGTCTGGTGAGAAGTGCTTTCG18680.3703373281390946No Hit
AGTCTGTAGATACTGTGGCTTTGGTGGAACGTTCTGCGTTACC18180.3604246587563565No Hit
AGTCTGCACGCTGGTTGGCGGAAGGGGTGGTCACGTTGTCACC16720.3314796641587613No Hit
AGTCTGCAGGTATAACTCTGTGAGTTGAATTCAATTATCACAG15790.31304209910686853No Hit
AGTCAGCAGTGGGGCCACCACCGACAGCATGCCGTCGCCACTG15410.30550847037598755No Hit
AGTCTGCAAACGGGCGGGTCACGCCTCTATACGGGTGATTTGG14880.29500104083028517No Hit
AGTCTGTAGCGGCAATCATTGACGACACGATAGCAGTATTCCA14300.28350234434630905No Hit
AGTCTGCAGGCCATCTATGTATGTTTTTATAGGTAAAATGTGT13270.26308224541786857No Hit
AGTCTGCAGATTAGCACGCCGTCTTTCATCGGCCGGATCGCCT13120.26010844460304716No Hit
AGTCTGCAGTGTGCTTCCTAGTCTGCATTTTTTTTTTTTTTTT12400.24583420069190431No Hit
AGTCTGCAGCTACGATGAATTAGATGAAATATTTGTTCATTTA10880.21569968576838056No Hit
AGTCTGCAAGGGTATTTCGGTAATTTAGAATTTGCAATTGTAG10660.2113381112399758No Hit
AGTCTGCATATCCAAGAGGCCTGGGGGCGATGGAATCAAACCA10520.20856256381280913No Hit
AGTCTGCAAGAGGCGTCAAACATTTTTTTCTTTTTAAAAAAAT10370.20558876299798776No Hit
AGTCCACATCTTCGTTAGGGAGCTATTATATTAGCCCTGGCTT10190.20202020202020202No Hit
AGTCTGCATGTCCAAAAACGGCCAACGCTTTCCATTTCGGCAA8310.16474856514110686No Hit
AGTCTGCGGTGAAAGGAGATCAAGGTTTTACTTCTGCGATGAC7700.15265510849416639No Hit
AGTCGGCAGAACCAGTGTTGCAACAAGATCTTGTACAATAAGC7380.14631100008921402No Hit
AGTCTGCAGATTATCACGCCGTCTTTCATCGGCCGGATCGCCT7300.14472497298797593No Hit
AGTCTGCAGCAAACAGTCCGACGATCACAGCAGTCTGCCGAGA7290.14452671960032118No Hit
AGTCTGCATGTAACGACGCGGCGCGTGCGCGGGCGGGGCCCGC7260.1439319594373569No Hit
AGTCTGCACCATTGAAACGGGAAAGCGGGATCAACTGATCATT6780.13441579682992832No Hit
AGTCTGCATGTTAGCAGAAACGATCGATCCGTAGCTTATGGTG6760.1340192900546188No Hit
AGTCTGCAATTAAGAAAAAGGGTATATGTGGATTCCTTGGAAC6750.13382103666696404No Hit
AGTCTGCAGTGTATGGTGGCAGGCACCTGTGGTCTCAGCTACT6740.1336227832793093No Hit
AGTCTGCAGGCCCATTGCTTTAAGACCCTACCAATTCTGCAAC6710.133028023116345No Hit
AGTCTGCAGTGCCGGTGCCCAGTTAATCAGGTCAACCAAGCTC6690.13263151634103548No Hit
AGTCTGCACAGGTATGAAGTCTCTCGTGGAATATGTGAGGCAG6550.1298559689138688No Hit
AGTCTGCAGTCGGAGATAAAGGGTCTTTGAGACTCCTAGTATG6450.12787343503732118No Hit
AGTCTGCAGTGAGAGGTAGAAGAGATCGAGGAAGATTATTTAG5770.11439220467679742No Hit
AGTCTGCAGCGCGAAAGACGCTCCTACAAATTCGAGAAGAGCT5470.10844460304715456No Hit
AGTCTGCAAGTGGCTTCTCCCGTACCCCGACAGACGGAGCAAG5450.10804809627184506No Hit
AGTCTGCACGCTGGGTTGGCGGAAGGGGTGGTCACGTTGTCAC5430.10765158949653551No Hit
AGTCTGCAAAACGATGCAAAAAAATGGGACTCGACATCAATAA5370.10646206917060694No Hit
AGTCTGCAGGTGGGATCAGTAAACGCTGTAGCGTCTAGAGCAC5220.10348826835578553No Hit
AGTCTACACCAAAGCAGGGATGAAGACGAAGATGAAGAGGAGG5190.10289350819282124No Hit
AGTCTGCATCTGAGGTCATCGGACAATCGAATTTCCAATTTCG5180.10269525480516649No Hit
AGTCTGCAGTCTTCAGTCCGACGATCGCGTGAGTCTGCATTTT5130.10170398786689269No Hit
AGTCTGCGTACAATGCAACGGCGGGCCAATCGAAAAAACGCAT5100.1011092277039284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG800.037.0091214
CTGACAC200.001838729437.0091214
CGTTAGG650.037.0091213
TAGCCGG251.2315744E-437.00545518
TAGAACC600.037.0054519
TGACACT200.001839625137.0054515
ACCCGCG308.2943425E-637.0054537
GCGAGAT200.001840521137.00178510
CGGGTGT200.001840521137.0017859
CCGGGAA200.001840521137.00178510
CGAACGG200.001840521137.0017859
ACGCACG200.001840521137.0017858
ATGGACG700.037.0017858
GCCGGGT251.2322934E-437.00178511
AAGCGTA251.2322934E-437.0017858
TACGGAC200.001840521137.00178511
TGACGCT200.001840521137.0017859
TGTGCGG355.620823E-737.00178511
CCTCCCG355.620823E-737.00178510
GAGCGTC251.2322934E-437.00178511
Produced by FastQC (version 0.11.5)