FastQCFastQC Report
Thu 26 May 2016
SRR2099099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263663
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCACTAGTTCGTATGCTCGGCAAAGATGAGCGAAACTATCAC13300.5044317936153347No Hit
GTCACTAGCCTACTCTCCCCCTCGCCGGGCCCGTCTCCCGACG8730.3311044780648024No Hit
GTAACAAGTCAGCCGGCTCGGCCCGGGCCGGCATGGACGCCAG8260.3132786928768921No Hit
GTCACTAGCTGAGGCGCTGCACACCCAGCGTCAGCAACTCGTC8210.31138233275051863No Hit
GTCACTAGTGGCAGGGTGAAATAGATCTACAGTGGAAATTTTT8010.3037968922450249No Hit
ATCACTTGTGGGGAGAGTCTTGTTCTATTGTCCAGGCTGAAGT6180.23439011161975704No Hit
GTCACTAGGGTTCGTCCGACGATCAGCTAGTCACTAGTTTTTT6060.2298388473164608No Hit
GTCACTAGAGGGGAGTTCTACCAGTCCGACGATCGTCAAGTCA5860.22225340681096703No Hit
GTCACTAGCAGTCGGAAAAGATGGGAGGAACACAATAAGTGTT5530.20973742997690234No Hit
GTCAATAGTGCGGTGCGTTCCTTGTTGACGGCGTAGCGCTGGG5510.20897888592635297No Hit
GTCACTAGAATGGGTGATATATAAAAAATTAAACATTTTTTAA5290.20063490137030981No Hit
GTCACTAGTGATGGCTGCTTCCATCTGGTTCCACAGCCCTCCT5130.19456654896591483No Hit
GTCACTAGCTTATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.19304946086481609No Hit
GTCACTAGAGCCACAATTACCTTAAAAATCGAGGTAAGGGTGA5010.19001528466261855No Hit
GGCACTACCGTGTGATCCTTTCTGCAAAGTCAGGACAATCCTG4660.17674076377800452No Hit
GTCACTAGTAAATGGCTTTGTTTTTTGAAAGCAAACATTTTTG4560.1729480435252576No Hit
GTCACTAGGTCGCAAAAGGAGTCGGAGCGAGAAACGTGATATA4470.16953459529778545No Hit
GTCACTAGGCCATGGAACAACAATTACTCTTGTCTTAAAAGAA4420.167638235171412No Hit
GACACCAGAGTCGTCGTCAGTTACCTTCTAACTTTAAGTCATC4300.16308697086811574No Hit
GTCACTAGACGTGCAGTCCGACGATCAATAAGTCACTAGTGAC4220.16005279466591824No Hit
GTCACTAGCTGAGGTCCGACGATCGCGGACTGTAGAACTCTGA4040.15322589821097385No Hit
GTCACTAGGACGCGTCACGCCCGCTGGCCGAGACACCACCCTT3860.14639900175602946No Hit
GTCTCTTGGTACAGGTGTGTCAACTGCTGTACGATCGTCGGAC3710.14070992137690916No Hit
GTCACTAGCGTGACAGTCCGACGATCGATTTGTCACTAGTTGA3660.1388135612505357No Hit
GTCACTAGGGCGCTTCTACAGTCCGACGATCCGTCGGACTGTA3610.13691720112416228No Hit
GTCACTAGGGTGAAATTGATTAAATATCAATTTCATTTCACCC3570.13540011302306354No Hit
GTCACTCGGGTTGCAGTCCGACGATCAAAAAGTCACTAGTTAC3540.13426229694723946No Hit
GTCACTAGGCCATGGGAACAACAATTACTCTTGTCTTAAAAGA3510.1331244808714154No Hit
GTCACTAGTGTCTTGCTAAAAAAGATTCAGCTTTTGGAGCATT3480.13198666479559135No Hit
GTCACTAGCGGGTTTGCAGTGAGCCGAGATCATGCCATTGCAC3440.1304695766944926No Hit
GTCACTAGATTACGTCCGACGATCGGAGTGTCACCACGATCGT3360.12743540049229507No Hit
GTCACTAGGGCGTTACAGTCCGACGATCACGCGGTCACTAGTG3350.1270561284670204No Hit
GTCACTAGTTTAAAGAAGGTCGAGGCTGCAGTGAGCCATCTGC3320.12591831239119636No Hit
GCAACTAGTCAGAGAGGATTGCTTGAGCCGAAGTGGTTGAGGC3280.12440122429009759No Hit
GTCACTAGTGTTTTCCGACGATCCAAAAGTCACTAGTGACAAA3170.12022923201207601No Hit
GTCACTAGAATTAGATGTATGTAGTATCTGGATTATTTTACAA3130.11871214391097726No Hit
GTCACTAGCAGTAGTTCTACAGTCGGACGATCGAAACGTCACT3100.1175743278351532No Hit
GCCCCTAGAGTCGGGAACATCCTAAAGAATGCTGAGTCATCAA3090.11719505580987852No Hit
ATCACTAGCCGCCATACGACAAACCCACAGCGAACATCATTCT3080.11681578378460383No Hit
GTCACCAACCGTACCACACGCTCAGACTCCTCCGGGGATGGGC3080.11681578378460383No Hit
GTCACTAGTTGGTGACCACATTTTCTTGTGAAAATGTACTCAT3070.11643651175932913No Hit
GTCACTAGCGAAGGCTCCCTGACAAGTGTCATGTTTTTCTGTA2980.11302306353185695No Hit
GTCACTAGTATCGCAGTCCGACGATCCAAAGGTCACTAGTGAC2960.11226451948130758No Hit
GTCACTAGGGCCAACAGTCCGACGATCGTTAGGTCACTAGTGA2960.11226451948130758No Hit
GTCACTAGCGCGATCCGACGATCTGTAGAACTCTGAACCCCTA2950.1118852474560329No Hit
GTCACTAGGTGGTATCTGAATGCTTACATAGCATTCAGATTGT2910.11036815935493414No Hit
GTCACTAGCCTGGGTTAGAAATGAGAGCATGTGTCTTATAAAG2820.10695471112746194No Hit
GTCACTAGTGCGTCAGTCCGACGATCAGTACGTCACTAGTGAC2810.10657543910218725No Hit
GTCACTAGGTCAAGTGGCACACGCCTTTAATCCCAGCACTCGG2790.10581689505163788No Hit
GTCACTAGCTTATGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.10278271884944039No Hit
GTCACTAGGGACTCTGCCCTTTGAAACTAACTTGCTTTTCAAG2700.10240344682416569No Hit
GTCACTAGAGAACGTCCAGTTCCCCGCCTCTGGCCTGTGAACC2670.10126563074834163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGAGC452.584784E-937.02828215
TGGCACC355.5758755E-737.02828215
ATGAGCT355.5758755E-737.02828216
GGGCGCA200.001832473337.02828214
CCGCACC200.001832473337.02828216
GCATCTT200.001832473337.02828216
TCATGCT251.2268909E-437.02125513
GACGGTT200.001834182737.02125518
CACCCAC308.255083E-637.02125518
GCTCCAC355.5841883E-737.02125518
TAATCTG308.255083E-637.02125513
ACGGTTT200.001835893337.01423319
AGGCTCC452.5993359E-937.0072112
CGCTTCG308.276611E-637.0072112
ACACGAT403.8076905E-837.00720612
TCATCGC200.001837605137.00720612
ACGAGGG200.001837605137.00720612
AACGTCC700.037.0001911
CGCATCA308.287392E-637.0001911
CCATCAG308.287392E-637.0001910
Produced by FastQC (version 0.11.5)