FastQCFastQC Report
Thu 26 May 2016
SRR2099098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387968
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTGTGTCACTCCATGTTTTTTTTTTTTTTTTTTTTTTTTT36460.9397682283074893No Hit
TAGCCATTATATTACAGTCCGACGATCAGTGCTAGCCATGTTT26220.6758289343451006No Hit
TAGCCATGGCCGGGGCGCCTTGCCCGATCAACGCCTGGTGGCG23390.6028847740019796No Hit
GAGCCATTTTCTAGGAAGTGAGCGGCGGTCACATCCGGCGGAT16730.43122113163972287No Hit
TAGCCATGGCGAAAATTGATCATGACCCCAATGGGAACTAAAA16530.42606606730451996No Hit
TAGCCATGCGCAGCCTTGCCAGAGTCGTCTCTCTTGCGTGCCT15530.4002907456285054No Hit
TAGCCATGGGATTGTGCACGCAGGTGCGAGTGGAATTCATGGA14830.38224802045529527No Hit
TAGCCATGGATTAAGAGAGAGAGAAAACGACTCCTTTAGAAAG13540.3489978554932366No Hit
TAGCCATGGGCTATGCAGCGAAGTTTCCTGCCTGCTGCAGTGG12680.3268310788518641No Hit
TAGCCATGCAGGAAGTCAGTTCAAAAACTGACTGGCATTTTCT12570.32399579346750246No Hit
TAGCCATGGCCAACATGCCTGGGCCTCCTGCGCGCCCTGGTGG12260.316005443747938No Hit
TAGCCATGTCCGTAGGTTAACCCAATGCCAGTGACAGATGAGG11380.2933231606730452No Hit
TAGCCATGGTCTTGGTCAAGAAAGTGAGCCATAAGACAGTTAA11260.29023012207192345No Hit
TAGCCATGGTGTTGTCGTGGCAGGTAGGCTGCGGAATGGTGTA10640.27424942263279445No Hit
TAGCCATGCTCGCTGATGACCCTCACAGCACCAACCCAAATTA9890.25491793137578356No Hit
TAGCCATGGGTCGGTAAAGCCCATAGGGCTTTAGGTCGATGCA9100.2345554272517321No Hit
TAGCCATGTGACAACTTGTGATGGAGGTAGCAGTCTCTTAAGA8930.23017362256680962No Hit
TAGCCATTATTTGTGCCATCATGGAATGGCCCCCGACATGTTG8820.22733833718244803No Hit
TAGCCATGACCGTGGGGGGGTCCACGAGTCAACCACAAGGTTG7860.2025940283734741No Hit
TAGCCATGACCGTGGGGGGTCCACGAGTCAACCACAAGGTTGG7810.20130526228967338No Hit
TAGCCACGCTTCCGGAAGACATTGTCAGGTGGGGAGTTTGGCT7500.19331491257010888No Hit
TAGCCGTGGTTGCGCAGCCTCCATGCGATCCTGGTCGGTGACC7360.18970636753546685No Hit
TACCCATGAGTATGACGATCCATGATAGCCATGTTTTTTTTTT7260.1871288353678654No Hit
TAGCCATGTAGTGGGAAGGAAGCGAGCTAATGACGTACCTATG6870.17707645991421972No Hit
TAGCCATGTGCGCCTTGGATGAGGTCTGCCTGGAGTTCCATGA6610.17037487627845596No Hit
CAGCAATGCGAAAGAGAGGTTTCTGTGTAGCAAGACGGGGTAA6570.16934386341141539No Hit
TAGCCATGCTGAGTTTGGCACGGTGCCGAGAATCTCACCGGTG6560.16908611019465522No Hit
TAGCCATGTAGACCAGGGGTACCAAAGTGATCCTCCATCTTAA6350.16367329264269218No Hit
AAGCCTTGTTTTCTGGATTGGTAGAGGAGCATTGTGTACGCAT6220.16032250082481028No Hit
TAGCCATATTACCAGGAAAAGGAGTCACGTGGTTCATGCCACA6090.15697170900692842No Hit
TAGCCATGCGCAGGGCCTTGCCAGAGTCGTCTCTCTTGCGTGC6090.15697170900692842No Hit
TAGCCATGAGGCGGTTCTAGCCCAACTCTTTTACTCCATGGCC5790.14923911250412403No Hit
TAGCCATGTACGCGCCGACCTGCGCGCCACCTTGCTGCACGCC5770.14872360607060378No Hit
TAGCCATATTCATGGTGTCTTTATAGCAATGTGACAGTTTTTC5630.14511506103596172No Hit
TAGCCATGAATATAATGCACACTGAAGGACCTGGAATATGGCG5620.1448573078192016No Hit
TAGCCATGAAACTGTTCTCTGATGTTCCAAAGTCCTGGAGTTG5140.13248515341471462No Hit
TAGCCATGAGTCTTGCAAGCCTTCGGTGATCACCCGATCACCC4890.126041322995711No Hit
TAGCCATAGTCGCGCCTGCTGAAACTGGTCCCATGTGATAGTG4880.12578356977895083No Hit
TAGCCATGGTGAAGAAAAGAAGAGATTGACTCAGATCAGAAGG4860.12526806334543056No Hit
CAGCAATGCGAAAGGAGAGGTTTCTGTGTAGCAAGACGGGGTA4640.11959749257670735No Hit
TAGCCATGGTTCATCTATGTGCATGAGGGAGGATAGCAAATTG4640.11959749257670735No Hit
GAGCCATTTTCTAGGGAAGTGAGCGGCGGTCACATCCGGCGGA4560.1175354668426262No Hit
TAGCCATGGCCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.11418467502474432No Hit
TAGCCATGAGAGTGGACGGCCCAAAGAATGTCATGCAGCTGCG4380.11289590894094359No Hit
TAGCCAATGCTGGGCTTCCTCCTCCATGGCCTCCCCCTAGATG4360.1123804025074233No Hit
TAGCCATGGCCGGGGGCGCCTTGCCCGATCAACGCCTGGTGGC4290.11057612999010227No Hit
TAGCCATGATTCTGTTAGGGCCCACACAAAAGAAACACCGTAG4170.10748309138898053No Hit
TAGCCATGGCTGTTCTGAGTGCTTTACTCCATGAACTGTCTAA4140.1067098317387001No Hit
TAGCCATGTTGAGCACACATGGGTTGGACTGAGTCTCCAAGCA4130.10645207852193996No Hit
TAGCCATGTGCCTGCCCATTGTTTCTACGGCCCGTACGAGGAC3930.10129701418673705No Hit
TATCCATGGATATGACCGAATGATGTGTATAATAGTAAGAGTG3930.10129701418673705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAC251.2292151E-437.0148313
CAACACG251.2292151E-437.0148313
CGTTACA600.037.0148313
CACAGCG200.001836814137.0148317
CGTGGGT200.001840307137.00051511
TAATAAG251.2320172E-437.00051512
GGGTGCG200.001840307137.0005158
GGGCTTA251.2320172E-437.0005158
GAATGCG1050.037.0005158
ACACCCG355.617876E-737.0005158
TAGCGTT403.8216967E-837.00051510
CAGTTAG251.2320172E-437.00051512
TAGCGTC200.001840307137.00051510
GACCGTG1900.037.0005158
TCCCTAA251.2320172E-437.00051520
CCGTCGA308.296987E-637.00051510
AGCGGAG403.8216967E-837.00051511
GGGACTT452.6102498E-937.0005158
ACTAGCG403.8216967E-837.00051510
CCGTACG308.296987E-637.00051532
Produced by FastQC (version 0.11.5)