FastQCFastQC Report
Thu 26 May 2016
SRR2099097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140171
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAAGCTGGTCAGATATATCAGTATTACTAACTGATAAAAGG16671.1892616875102553No Hit
CGTAAGCTCTACTAGTGACCCTTCTCGTTGAAACCTTCCCTGG8090.5771521926789421No Hit
CGTAAGCTCAGGGGGCTTTGGGACTGGCCAGGAGGGGACTGAG8050.5742985353603813No Hit
CGTAAGCTCGATTGTCGTAAGCTTTTTTTTTTTTTTTTTTTTT5930.4230546974766535No Hit
CGTAAGCTCGGATTCTCAGCTACTTTGGGAGGCTGAGGCAGGT5080.3624144794572344No Hit
CGTAAGCTTACTGAAGGAATGCAATGTAAAGTATTGGAAAGGA4600.3281705916345036No Hit
CGTAAGCTGCTAAGCAGTTCATGATCTTAGAGGATATTCTAAA4430.3160425480306197No Hit
CGTAAGCTTCTGGACAGTCCGACGATCGTCGGACTGTAGAACT4360.31104864772313817No Hit
CGTAAGCTGCTATGGGCAGGTAGGTCACATGAGGTCAAGAGTT4150.29606694680069345No Hit
CGTAAGCTCCAGATACAGTCCGACGATCCGGTATGTAGAACTC4060.2896462178339314No Hit
CGTAGCCTAGGCTCAGACACGGCATCGTCGAAGGAAATCGTCG3870.27609134557076714No Hit
CGTAAGCTCTACTGTGACCCTTCTCGTTGAAACCTTCCCTGGC3670.2618230589779626No Hit
CGTAAGCTGATCAGGTTCAGACCGGAGCAATCCAGGTCGGTTT3630.25896940165940174No Hit
CGTAAGCTGGATTCGCAGCGCTCTCCTCTGCCCATCCCCACTT3530.25183525836299947No Hit
CGTAAGCTACCGTAAATACAGTTCTCATAGGGTGTACGGTCTA3530.25183525836299947No Hit
CGTAAGCTGTGTGGCAGGATATGACTATTAAGTTGGGGCATAC3530.25183525836299947No Hit
CGTAAGCAGCCAGTGAAGGAATGACCTGTCAAAGCCATAAGAA3490.2489816010444386No Hit
CGTAAGCTTACACCAGTCCGACGATCACACGCGTAAGCTCGTC3440.24541452939623745No Hit
CGCTAGCTCTTTCGACAGGGTCGTGGACTGCGTGGTCCCAGGC3330.23756697177019498No Hit
CGTAAGCTCGCAAGGGAGTGGAAGGTACTCACATGGTCCAGGA3270.23328648579235362No Hit
CGTAAGCTGTGCAGGGAGTGGGGGTGGGTGGGTAAGGGGGACT3140.2240120995070307No Hit
CGTCAGATAATTGGGAACCGACCTGAGGAAGGTCCCCGACGGT3060.2183047848699089No Hit
CGTAAGCTGATCAGTTCAGACCGGAGCAATCCAGGTCGGTTTC3040.21687795621062844No Hit
CGTAAGCTTCCACGTCCGACGATCGTTTTCGTAAGCTTACGGT2850.20332308394746418No Hit
CGTAAGCTCTTGAAGTTGAACATGGGTCAGTCGGTCCTGAGAG2840.20260966961782395No Hit
CGTAAGCTCCTTTTCCGACGATCTTTCGCTTAAGCTTACGAAA2830.20189625528818372No Hit
CGTAAGCTGCCTGGCAAACTGAATCAAAAAATACATCAAGAAG2830.20189625528818372No Hit
CGTAAGCTCGATTGGTCGTAAGCTTTTTTTTTTTTTTTTTTTT2780.1983291836399826No Hit
CGTAAGCTCGCAATCTGGCATGCTAACTAGTTACGCGACCCCC2510.17906699673969653No Hit
CGTAAGCTTGGACGTTCTACAGTCCGACGATCATTGTCGTAAG2470.17621333942113562No Hit
CGTAAGCTTTGATGTCCGACGATCCTCGTCGTACTGTAGAACT2370.16907919612473335No Hit
CGTAAGCTCCAACGGCTGAGCAAGCCAGGTGAGGCAAGGCAGT2320.16551212447653224No Hit
CGTAAGGTATACCGATCGTGGACTGTAGAACTCTGAACCCCTA2300.1640852958172518Illumina DpnII expression Adapter 1 (95% over 21bp)
CGTAAGCTGGGTCGCACATCAAACAGCTTATCCACCACAATTA2280.16265846715797133No Hit
CGTAAGCTTATGTCAGTCCGACGATCGTCGGACTGTAGAACTC2220.15837798118012997Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGTAAGCTCGATAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.15409749520228863No Hit
CGTAAGCTTACTGCTTTACATTGCAAGGAATGCAATGTAAAGT2090.14910359489480707No Hit
CGTAAGCTCGGATTCAGCTACTTTGGGAGGCTGAGGCAGGTGA2090.14910359489480707No Hit
CGTAAGCTACCTGGCTGCGCCGAGGAAGCGAACGCAGAAAAAG1970.14054262293912437No Hit
CGTAAGCTTTCGCTGTGCACTCCCTATATAAAACCTATTTGAT1970.14054262293912437No Hit
CGTAAGCTATCCTACAGTCCGACGATCCGCGTCGTACTGTAGA1960.13982920860948414No Hit
CGTAAGCTGCTGTGGCTGAGGAGGCTGATGAACAAGCCAATGC1920.13697555129092323No Hit
CGTAAGCTGGCGTGCTCAGTTTACTCATTTTAATTACAAATAA1910.136262136961283No Hit
CGTAAGCTTACTGTACTTTACATTGCAAGGAATGCAATGTAAA1890.13483530830200255No Hit
CGTAAGCTTACTGCAAGGAATGCAATGTAAAGTATTGGAAAGG1870.1334084796427221No Hit
CTTTAGCTAGCTGGAGCCGGGGCTTGGGCACAGACATGAGGCA1850.13198165098344167No Hit
CCTAAGCGTCGAGCAGGAATCCCAGTGCCAGAGGTCAGGCAGG1840.13126823665380144No Hit
CGTAAGCTGGGAAGGTGGAGCCGAAAGGCTTAATTCTTATTAA1820.12984140799452099No Hit
CATATGCTTCGATGTCCGACGATCTCGAACAGGTTGTCAGACT1820.12984140799452099No Hit
CGTAAGCTTTGCGTCTGGCAACAGGTCACAGCAGACGGCTTAC1800.12841457933524053No Hit
CGTAAGCTACGGTACAGTCCGACGATCTACTACGTAGCTGATC1790.1277011650056003No Hit
CGTAAGCTCGGGAGAGTTCTACAGTCCGACGATCATCGTCGTA1780.12698775067596008Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGTAAGCCTCGAGGTTTACAGTCCTGACTGAAAGTCACCCTGC1760.12556092201667962No Hit
CGTAAGCTTGAACGAGAGAGAGAGAGAGAGAGGCTGGGCGCGG1750.1248475076870394No Hit
CGTAAGCTGGCGTCTCAGTTTACTCATTTTAATTACAAATAAA1740.12413409335739918No Hit
CGTAAGCTTTGATTTTAAATAGCCAAGGTTTCAAATATAAAGC1730.12342067902775895No Hit
CGTAAGCTATCCCGTAAGCTTTTTTTTTTTTTTTTTTTTTTTT1720.12270726469811873No Hit
CGTAAGCTTAATTCAGTCCGACGATCGTCGGACTGTAGAACTC1700.12128043603883829Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGTAAGCTGTGTGGGCAGGATATGACTATTAAGTTGGGGCATA1690.12056702170919806No Hit
CGTAAGCTTTAATGCGAGGTCAGGAGATCGAGACCATCCTGGC1680.11985360737955782No Hit
CGTAAGCTGTGCCGTGTGGTGGCACACTCCTGTAATCCCAGCT1640.11699995006099692No Hit
CGTAAGCTTTGGAGTCCGACGATCTTCGTCGTACTGTAGAACT1620.11557312140171648No Hit
CGTAAGCTAACTTGACAGAGTACTTGGAGGAGAGGAGGGTCAA1590.11343287841279581No Hit
CGTAAGCTTACTGTTTACATTGCAAGGAATGCAATGTAAAGTA1590.11343287841279581No Hit
CGTAAGCTGTCCCATGCCCCAGAATTTGCCAAGTTATCACCAA1590.11343287841279581No Hit
CGTAAGCTCCTGTAACATATAACTGAACTCCTCACACCCAATT1550.1105792210942349No Hit
CGTAAGCTCAGATGAAACAAGGGTAACAAGGATGGGATAGGGG1550.1105792210942349No Hit
CGTAAGCAAGCTACAGTCCGACGATCAGCTCGGAAAAACAGTC1540.10986580676459468No Hit
CGAAAGCGTAGGGGGCCTGCTCCAGCTTGCCGGGCTGGCCCTG1520.10843897810531422No Hit
CGTAAGCTGCAGTGTGCGGACGCCAGAGCCGAGCCCGCGTCGC1500.10701214944603378No Hit
CGTTGGCTTCGATGTGACTACGTTCGAGATCGTCGGACTGTAG1470.1048719064571131No Hit
CGTAAGCTCGGTGTTGCAGTGAGCCAAGCTTCCAAGCTTGCAC1470.1048719064571131No Hit
CGTAAGCTGCTATGGCAGGTAGGTCACATGAGGTCAAGAGTTC1460.10415849212747287No Hit
CGTAAGCTAACCTACAGTCCGACGATCGTAAACGTAAGCTTAC1440.10273166346819243No Hit
CGTAAGCTATAGAAGCAGATTCCTCATTAGACCTGGAGGTGCA1430.1020182491385522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCA308.243582E-637.0107087
CTTCGAT1000.037.0107087
AGGTATA551.2732926E-1137.0107085
AGGTAGT200.00183381837.0107085
CGTGGAG200.00183381837.0107087
TAATAAG200.00183381837.0107088
CTGTCGA308.243582E-637.0107087
TCGCAAC308.243582E-637.0107088
GATAATT750.037.0107086
TGCTTCG200.00183381837.0107085
CCTAGTC251.2260111E-437.0107086
CTATCGC850.037.0107087
GCGGTAC251.2260111E-437.0107086
CTATCCC800.037.0107087
ATAATTG750.037.0107087
CTTCACA200.00183381837.0107087
TGGGTCG308.243582E-637.0107088
GCTGCGG600.037.0107086
GCTGCCG800.037.0107086
AAGGTAT551.2732926E-1137.0107084