SRR2099097_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	140171
Sequences flagged as poor quality	0
Sequence length	43
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTAAGCTGGTCAGATATATCAGTATTACTAACTGATAAAAGG	1667	1.1892616875102553	No Hit
CGTAAGCTCTACTAGTGACCCTTCTCGTTGAAACCTTCCCTGG	809	0.5771521926789421	No Hit
CGTAAGCTCAGGGGGCTTTGGGACTGGCCAGGAGGGGACTGAG	805	0.5742985353603813	No Hit
CGTAAGCTCGATTGTCGTAAGCTTTTTTTTTTTTTTTTTTTTT	593	0.4230546974766535	No Hit
CGTAAGCTCGGATTCTCAGCTACTTTGGGAGGCTGAGGCAGGT	508	0.3624144794572344	No Hit
CGTAAGCTTACTGAAGGAATGCAATGTAAAGTATTGGAAAGGA	460	0.3281705916345036	No Hit
CGTAAGCTGCTAAGCAGTTCATGATCTTAGAGGATATTCTAAA	443	0.3160425480306197	No Hit
CGTAAGCTTCTGGACAGTCCGACGATCGTCGGACTGTAGAACT	436	0.31104864772313817	No Hit
CGTAAGCTGCTATGGGCAGGTAGGTCACATGAGGTCAAGAGTT	415	0.29606694680069345	No Hit
CGTAAGCTCCAGATACAGTCCGACGATCCGGTATGTAGAACTC	406	0.2896462178339314	No Hit
CGTAGCCTAGGCTCAGACACGGCATCGTCGAAGGAAATCGTCG	387	0.27609134557076714	No Hit
CGTAAGCTCTACTGTGACCCTTCTCGTTGAAACCTTCCCTGGC	367	0.2618230589779626	No Hit
CGTAAGCTGATCAGGTTCAGACCGGAGCAATCCAGGTCGGTTT	363	0.25896940165940174	No Hit
CGTAAGCTGGATTCGCAGCGCTCTCCTCTGCCCATCCCCACTT	353	0.25183525836299947	No Hit
CGTAAGCTACCGTAAATACAGTTCTCATAGGGTGTACGGTCTA	353	0.25183525836299947	No Hit
CGTAAGCTGTGTGGCAGGATATGACTATTAAGTTGGGGCATAC	353	0.25183525836299947	No Hit
CGTAAGCAGCCAGTGAAGGAATGACCTGTCAAAGCCATAAGAA	349	0.2489816010444386	No Hit
CGTAAGCTTACACCAGTCCGACGATCACACGCGTAAGCTCGTC	344	0.24541452939623745	No Hit
CGCTAGCTCTTTCGACAGGGTCGTGGACTGCGTGGTCCCAGGC	333	0.23756697177019498	No Hit
CGTAAGCTCGCAAGGGAGTGGAAGGTACTCACATGGTCCAGGA	327	0.23328648579235362	No Hit
CGTAAGCTGTGCAGGGAGTGGGGGTGGGTGGGTAAGGGGGACT	314	0.2240120995070307	No Hit
CGTCAGATAATTGGGAACCGACCTGAGGAAGGTCCCCGACGGT	306	0.2183047848699089	No Hit
CGTAAGCTGATCAGTTCAGACCGGAGCAATCCAGGTCGGTTTC	304	0.21687795621062844	No Hit
CGTAAGCTTCCACGTCCGACGATCGTTTTCGTAAGCTTACGGT	285	0.20332308394746418	No Hit
CGTAAGCTCTTGAAGTTGAACATGGGTCAGTCGGTCCTGAGAG	284	0.20260966961782395	No Hit
CGTAAGCTCCTTTTCCGACGATCTTTCGCTTAAGCTTACGAAA	283	0.20189625528818372	No Hit
CGTAAGCTGCCTGGCAAACTGAATCAAAAAATACATCAAGAAG	283	0.20189625528818372	No Hit
CGTAAGCTCGATTGGTCGTAAGCTTTTTTTTTTTTTTTTTTTT	278	0.1983291836399826	No Hit
CGTAAGCTCGCAATCTGGCATGCTAACTAGTTACGCGACCCCC	251	0.17906699673969653	No Hit
CGTAAGCTTGGACGTTCTACAGTCCGACGATCATTGTCGTAAG	247	0.17621333942113562	No Hit
CGTAAGCTTTGATGTCCGACGATCCTCGTCGTACTGTAGAACT	237	0.16907919612473335	No Hit
CGTAAGCTCCAACGGCTGAGCAAGCCAGGTGAGGCAAGGCAGT	232	0.16551212447653224	No Hit
CGTAAGGTATACCGATCGTGGACTGTAGAACTCTGAACCCCTA	230	0.1640852958172518	Illumina DpnII expression Adapter 1 (95% over 21bp)
CGTAAGCTGGGTCGCACATCAAACAGCTTATCCACCACAATTA	228	0.16265846715797133	No Hit
CGTAAGCTTATGTCAGTCCGACGATCGTCGGACTGTAGAACTC	222	0.15837798118012997	Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGTAAGCTCGATAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	216	0.15409749520228863	No Hit
CGTAAGCTTACTGCTTTACATTGCAAGGAATGCAATGTAAAGT	209	0.14910359489480707	No Hit
CGTAAGCTCGGATTCAGCTACTTTGGGAGGCTGAGGCAGGTGA	209	0.14910359489480707	No Hit
CGTAAGCTACCTGGCTGCGCCGAGGAAGCGAACGCAGAAAAAG	197	0.14054262293912437	No Hit
CGTAAGCTTTCGCTGTGCACTCCCTATATAAAACCTATTTGAT	197	0.14054262293912437	No Hit
CGTAAGCTATCCTACAGTCCGACGATCCGCGTCGTACTGTAGA	196	0.13982920860948414	No Hit
CGTAAGCTGCTGTGGCTGAGGAGGCTGATGAACAAGCCAATGC	192	0.13697555129092323	No Hit
CGTAAGCTGGCGTGCTCAGTTTACTCATTTTAATTACAAATAA	191	0.136262136961283	No Hit
CGTAAGCTTACTGTACTTTACATTGCAAGGAATGCAATGTAAA	189	0.13483530830200255	No Hit
CGTAAGCTTACTGCAAGGAATGCAATGTAAAGTATTGGAAAGG	187	0.1334084796427221	No Hit
CTTTAGCTAGCTGGAGCCGGGGCTTGGGCACAGACATGAGGCA	185	0.13198165098344167	No Hit
CCTAAGCGTCGAGCAGGAATCCCAGTGCCAGAGGTCAGGCAGG	184	0.13126823665380144	No Hit
CGTAAGCTGGGAAGGTGGAGCCGAAAGGCTTAATTCTTATTAA	182	0.12984140799452099	No Hit
CATATGCTTCGATGTCCGACGATCTCGAACAGGTTGTCAGACT	182	0.12984140799452099	No Hit
CGTAAGCTTTGCGTCTGGCAACAGGTCACAGCAGACGGCTTAC	180	0.12841457933524053	No Hit
CGTAAGCTACGGTACAGTCCGACGATCTACTACGTAGCTGATC	179	0.1277011650056003	No Hit
CGTAAGCTCGGGAGAGTTCTACAGTCCGACGATCATCGTCGTA	178	0.12698775067596008	Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGTAAGCCTCGAGGTTTACAGTCCTGACTGAAAGTCACCCTGC	176	0.12556092201667962	No Hit
CGTAAGCTTGAACGAGAGAGAGAGAGAGAGAGGCTGGGCGCGG	175	0.1248475076870394	No Hit
CGTAAGCTGGCGTCTCAGTTTACTCATTTTAATTACAAATAAA	174	0.12413409335739918	No Hit
CGTAAGCTTTGATTTTAAATAGCCAAGGTTTCAAATATAAAGC	173	0.12342067902775895	No Hit
CGTAAGCTATCCCGTAAGCTTTTTTTTTTTTTTTTTTTTTTTT	172	0.12270726469811873	No Hit
CGTAAGCTTAATTCAGTCCGACGATCGTCGGACTGTAGAACTC	170	0.12128043603883829	Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGTAAGCTGTGTGGGCAGGATATGACTATTAAGTTGGGGCATA	169	0.12056702170919806	No Hit
CGTAAGCTTTAATGCGAGGTCAGGAGATCGAGACCATCCTGGC	168	0.11985360737955782	No Hit
CGTAAGCTGTGCCGTGTGGTGGCACACTCCTGTAATCCCAGCT	164	0.11699995006099692	No Hit
CGTAAGCTTTGGAGTCCGACGATCTTCGTCGTACTGTAGAACT	162	0.11557312140171648	No Hit
CGTAAGCTAACTTGACAGAGTACTTGGAGGAGAGGAGGGTCAA	159	0.11343287841279581	No Hit
CGTAAGCTTACTGTTTACATTGCAAGGAATGCAATGTAAAGTA	159	0.11343287841279581	No Hit
CGTAAGCTGTCCCATGCCCCAGAATTTGCCAAGTTATCACCAA	159	0.11343287841279581	No Hit
CGTAAGCTCCTGTAACATATAACTGAACTCCTCACACCCAATT	155	0.1105792210942349	No Hit
CGTAAGCTCAGATGAAACAAGGGTAACAAGGATGGGATAGGGG	155	0.1105792210942349	No Hit
CGTAAGCAAGCTACAGTCCGACGATCAGCTCGGAAAAACAGTC	154	0.10986580676459468	No Hit
CGAAAGCGTAGGGGGCCTGCTCCAGCTTGCCGGGCTGGCCCTG	152	0.10843897810531422	No Hit
CGTAAGCTGCAGTGTGCGGACGCCAGAGCCGAGCCCGCGTCGC	150	0.10701214944603378	No Hit
CGTTGGCTTCGATGTGACTACGTTCGAGATCGTCGGACTGTAG	147	0.1048719064571131	No Hit
CGTAAGCTCGGTGTTGCAGTGAGCCAAGCTTCCAAGCTTGCAC	147	0.1048719064571131	No Hit
CGTAAGCTGCTATGGCAGGTAGGTCACATGAGGTCAAGAGTTC	146	0.10415849212747287	No Hit
CGTAAGCTAACCTACAGTCCGACGATCGTAAACGTAAGCTTAC	144	0.10273166346819243	No Hit
CGTAAGCTATAGAAGCAGATTCCTCATTAGACCTGGAGGTGCA	143	0.1020182491385522	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCA	30	8.243582E-6	37.010708	7
CTTCGAT	100	0.0	37.010708	7
AGGTATA	55	1.2732926E-11	37.010708	5
AGGTAGT	20	0.001833818	37.010708	5
CGTGGAG	20	0.001833818	37.010708	7
TAATAAG	20	0.001833818	37.010708	8
CTGTCGA	30	8.243582E-6	37.010708	7
TCGCAAC	30	8.243582E-6	37.010708	8
GATAATT	75	0.0	37.010708	6
TGCTTCG	20	0.001833818	37.010708	5
CCTAGTC	25	1.2260111E-4	37.010708	6
CTATCGC	85	0.0	37.010708	7
GCGGTAC	25	1.2260111E-4	37.010708	6
CTATCCC	80	0.0	37.010708	7
ATAATTG	75	0.0	37.010708	7
CTTCACA	20	0.001833818	37.010708	7
TGGGTCG	30	8.243582E-6	37.010708	8
GCTGCGG	60	0.0	37.010708	6
GCTGCCG	80	0.0	37.010708	6
AAGGTAT	55	1.2732926E-11	37.010708	4

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content