FastQCFastQC Report
Thu 26 May 2016
SRR2099096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285675
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATACTGCTACATCAGCACTGTGGCTGGTACGTGTTCTTT24900.8716198477290626No Hit
ATCGGTGTAGTGGGTGGTTTCATGCGAGCCAAACTCTCCGATA11230.39310405180712343No Hit
ATCGGAACCTTTTGCACCTCCAAGGACAAAATAGGCAGGCAGT10470.36650039380414806No Hit
ATCGGTACTGGACGTGAAGAGAAGCAGTGTGGAGATGTGGAGA9130.319593944167323No Hit
ATCGGTACGATTAAAAGAATCAGGAAAACTACGAGAGCTCCTG8910.3118928852717249No Hit
ATCGGTCGTAGCCGTGCGGTCGCGCTGGTCGAGACGCGCTCGG7640.2674367725562265No Hit
ATCGGTACAGGCAATCACCGGAGGTCAGGAGTTCGAGTCCAGC7460.2611359061871007No Hit
ATCGGTACCGCTTGGTACTTTTTTTTTTTTTTTTTTTTTTTTT7370.2579854730025379No Hit
ATCGGAATAGCTAACCTGCGGATGATACGCGAGATGCCAATCT7080.247834077185613No Hit
ATCGGTACGCAGGGGACGATCGCAGTATCGGTACTTTTTTTTT6990.24468364400105017No Hit
ATCGGTACAGCCAAGCTGGCCAGTTGTTCGCGCAGCTTTGGAT6600.23103176686794435No Hit
ATCGGTACTGCGGGGAGAATTACTAGGTAAGAGTCCAGCATCT6320.22123041918263758No Hit
ATTGGCACAGCTTATTTTGAGTAACCATTAAAAGCCACCCTCC5940.2079285901811499No Hit
ATCAGTTCTGGCGCGCCGACCGCGCCGCATCGCCCGCGCTCGC5840.20442810886496893No Hit
ATCGGTACGCAGGGACGATCGCAGTATCGGTACTTTTTTTTTT5780.20232782007526037No Hit
ATCGGTTGGGTGAAGGGCCCTGCACGTCAAGCAATGCGCATTG5670.19847729062746128No Hit
ATCGGTAGGGGATGTCGACGAGCACGTCAAAATCGTCCTGCCA5540.19392666491642602No Hit
ATCGGTACGCTCCAAAAAAACAGAGGCCCCAAAAAGGCCTCTT5350.18727575041568217No Hit
ATCGGTATACAGGCAGGACCCAAGCCCAAACCGGAAAAGTTGT5180.1813249321781745No Hit
ATCTGTAGCGAGACGCGAACCGCGCCTACGTCATGGAAACAGG5160.1806248359149383No Hit
ATATGTACTCGATCCGCGGCGGCGGATCGCGCAGGCGCTCGAC4750.16627286251859633No Hit
ATCGGTACAGCCATTTTCTTGTAGCTGGCCAGTTGTTCGCGCA4540.15892185175461626No Hit
ATCGGTACCCCCAAGAAGGAACGGGAGAAGGAGATCAGTGATG4340.1519208891222543No Hit
ATCGGAACCTTTTGGCACCTCCAAGGACAAAATAGGCAGGCAG4300.15052069659578193No Hit
ATCGGTACGCAGCGTAGATTATAGTTGCTCTTGCCGGAAGGGG4230.14807035967445523No Hit
ATCGGTACGCAGTGGAAGATGACTGAAATCTCTTCCTACTTTT3980.1393191563840028No Hit
ATCGGTACGCATCGGAGGAAGTCTCAGGGCCCATGAAGGGCAG3760.13161809748840467No Hit
TTCGGCACGCTGCCGCGGGTCCGTTGCGGTGGCGTGACCGACA3750.13126804935678654No Hit
ATCGGTACCCTTCCTATACTTGGCAAAAATTAGCCAAGTATAG3640.12741751990898748No Hit
ATCGGTACGTTGTGGTTATAAAAAAATAAAACAGGCTGGGAGC3590.125667279250897No Hit
ATCGGTACGGAGAAGAAAACGGTATTTAAAGCGGATCACTCTT3510.12286689419795221No Hit
ATCGGTACTTGGCAGAAAAGATTGCAATATCAAGTCCAGCAAA3480.12181674980309794No Hit
ATCGGTACCCTTCTACTTGGCAAAAATTAGCCAAGTATAGTGG3430.12006650914500744No Hit
ATCGTTACCTTTGGTTAATTCTGGATTTGTCGGGGACATGCAA3360.11761617222368075No Hit
ATCGGTACCGAATCAGTCCGACGATCAACTGATCGGTACTGTA3350.11726612409206265No Hit
ATCGGTCGTAGCCGGTGCGGTCGCGCTGGTCGAGACGCGCTCG3350.11726612409206265No Hit
ATCGGTGCATAGAGCACTTTGGGAGGCTGAGGCGGATGGATCA3340.11691607596044455No Hit
ATCGGTACGTAGCATTCACGGGAAAGCCACTGGACTGAGTTTC3220.11271549838102739No Hit
ATCTATACCATGCCAGTCCGACGATCCATGGATCGTCGGACTG3200.11201540211779121No Hit
ATCGGTACTGGACGGTGAAGAGAAGCAGTGTGGAGATGTGGAG3190.11166535398617311No Hit
ATCGGTACGTGTCCTTATAACGGATTTAAAGAAATCCGTTATA3160.11061520959131879No Hit
ATCGGTACGACTGATACAGGACTCTTTCGAGGCCCTGTAATTG3130.10956506519646451No Hit
ATCGGTACTGTCTGGCTGCTTGACAGCAGGATGGGGGTCGTGG3010.10536448761704735No Hit
ATCGGTACGGGCCGAGAACTACCCCCACACTCGCCCAGGCAGG2940.10291415069572066No Hit
ATCGGTACATACAGGCAGCCTGAACATCAATGAATTTAAGGAA2900.10151395816924828No Hit
ATCGGTACTTTCCGGGGTATGCCATGCTGGTACTGAGCGCGAT2880.10081386190601206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG308.276131E-637.0091067
TACCCGC1100.037.0091066
TACTTCG1250.037.0091066
ACGCATA308.276131E-637.0091067
ACCATGG452.5993359E-937.0091067
ACGCAAC200.001837399137.0091067
ACCGCTT1200.037.0091067
ACCGCTG355.6007593E-737.0091067
ACCGCGA200.001837399137.0091067
CGTGATA355.6007593E-737.0091068
CGTTGTA403.8078724E-837.0091068
ACTCTCG403.8078724E-837.0091067
TACGGTT1500.037.0091066
ATATGCG452.5993359E-937.0091065
TACGGCA1400.037.0091066
TACGGAA551.2732926E-1137.0091066
TATGCGG452.5993359E-937.0091066
ACGGGGC200.001837399137.0091067
ACGGGCC551.2732926E-1137.0091067
ACGATTA850.037.0091067
Produced by FastQC (version 0.11.5)