FastQCFastQC Report
Thu 26 May 2016
SRR2099095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244881
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACAGTTGAATGCGAAATGACACAAAATAGAACCTGCAAAT22800.9310644762149779No Hit
GCTACAGTCTGCTGTCCGACGATCGGCTTGCTACAGTTTTTTT20410.8334660508573553No Hit
CCGACAGTCTCCCCACGGATGGCCGCGACGCTGGCGGCGATCT16420.670529767519734No Hit
TCGACAAGGCGACGGATGCGCCCGGCATCGGGATCGAGGTAGC11480.46879913100648885No Hit
GCTACAGTGGTGTGCAACATATACCAGCTTCCCCACTGGGTGT10960.44756432716298933No Hit
GCTACAGTCTGCTGGTCCGACGATCGGCTTGCTACAGTTTTTT9850.40223618818936546No Hit
TCGACAGTATTGCGGCAAGTTACTTAACCTCTCTGAGTCTCTG8640.35282443309199163No Hit
GCTACAGTATAATGTCCGACGATCATCCTGCTACAGTTTTTTT7580.30953810218024264No Hit
TCGACAGTTCTGAGTAGAAGATATGCAGCTTGGTCTTCATGTG6950.28381132060061826No Hit
TCGACGATGCTCTGGTGGGGCCGTCCTCGCCCACGCCCACCGA6900.28176951253874327No Hit
TCGACAACCGCTAGAAACCTATCGGAACAGATTGTGAAATCTG6550.26747685610561867No Hit
TCGAAAGTTTTTCTATCCATCAGGACCATCTAATGTGAAGCGT6210.25359256128486896No Hit
GCTACAGTCTACTGTCTACTTGCTACAGTTTTTTTTTTTTTTT6170.25195911483536904No Hit
TCGACAGTACCAGGAAAGAAGCAAATGAAAAACAATCTAAATT5370.2192901858453698No Hit
TCGACAGTCGCTAGTCCGACGATCATACGTCGGACTGTAGAAC5210.21275640004736995No Hit
GCTACAGTTTGTTCAGTCCGACGATCAAAAACTGCTAGTTTTT5180.21153131521024499No Hit
GCTACAGTAGCTTGTCCACTGTAGGAGGTGGAATATGGCAAGA5120.20908114553599505No Hit
GCTACAGTGATGTCCTAGGTTTTGCATATGACCTGCAGCCTAA5050.2062226142493701No Hit
TCGACAGCGACATCCCAATCCGGCGGCCAGGACCGCAAACGCA5030.20540589102462015No Hit
TTGCCAGTGGGGCGGCGCCCTTTTCCACCGTCAACATCTTCAC4790.19560521232762038No Hit
GCTACAGTGTGATGATCTGCGTGCTACAGTTTTTTTTTTTTTT4740.1935634042657454No Hit
GCTACAGTACCCTGGCAGCTTTGGATCGCCGAAATCCTGGTCT4710.19233831942862045No Hit
GCTACAGTATCCTGCGTGGTGGACGTCCAAACCGTGGCAGCAG4690.19152159620387046No Hit
TCGACAGTCGCATATTATCAGCTTAGCCCTGACCAGAATCGGA4670.19070487297912048No Hit
TCGACAGTCCGCAATTGCTGAAAAAATTCCAAATACGTCCATG4630.1890714265296205No Hit
GCTACAGTCTTTTGTTAGAAGAATATTGTGAAAATGACCACAT4620.18866306491724552No Hit
TTGCCAGTGGGGCGCGCCCTTTTCCACCGTCAACATCTTCACG4610.18825470330487054No Hit
TCGACAAGGCGACGGGATGCGCCCGGCATCGGGATCGAGGTAG4580.18702961846774557No Hit
GCTACAGTGATGTGGTTGCGGTGAGCCGAGATCGTGCCATTGC4550.18580453363062058No Hit
TCGACAGTTCATGGGAGAGAGGTAAGAGATGAACTACTGGAGG4200.17151187719749592No Hit
TCGACAGTCCAGCGGTCGCCAAGCGAAAGATGAGCGACCCTCA4000.16334464494999612No Hit
GCTACAGTTCAGTAGGAGCAGTGGAGCCAGCTCCCTCACCCCT3830.1564024975396213No Hit
GCTACAGTACTATCCTGAGTAGCTGGGACTACAGGCGCCTGCC3710.1515021581911214No Hit
GCTACAGTGTCATTCCGACGATCATCGGACTGTAGAACTCTGA3680.15027707335399643Illumina DpnII expression Sequencing Primer (95% over 24bp)
TCGTCACTCATGACATTCCGACGATCCATGATCAGCAAGTCAG3660.14946035012924644No Hit
GCTACAGTATAGTTGATCGACTGGTGATAAAGACGATAATAGC3510.1433349259436216No Hit
TCGACACTAGCACCCGAGCCCTCCATGGAGCCAGGCATCTGTT3400.1388429482074967No Hit
ACTACAGTGTGGTGCCGGGCCTCGCCCGGCGCATGGGCCCGCA3390.1384345865951217No Hit
TCGACAGTGATTCGTCCGACGATCTAGGATCGACAGACTGTAG3300.1347593320837468No Hit
GCTACAGTGCTTTGTGCACGCAGGTGCGAGTGGAATTCATGGA3260.13312588563424685No Hit
GCTACAGTATAGTGGCCAATGGGTTATCTGGACCAAAGGTGGG3220.13149243918474687No Hit
TCGACAGTCCAGCGTCGCCAAGCGAAAGATGAGCGACCCTCAT3190.1302673543476219No Hit
GCTACAGTTCTTTGCCGAAGTGGGAGGATCACTTGAGCTCAGG2990.1221001221001221No Hit
TCAACATTTCGAAACAGTCCGACGATCTCGAAAAAGAAAGCAG2970.12128339887537212No Hit
GCTACAGTCCGCTGTGAGCTGTAGCCTGAGGGACGTGAGGGAC2910.11883322920112217No Hit
TCGACAGTATTGCGGGCAAGTTACTTAACCTCTCTGAGTCTCT2890.11801650597637219No Hit
TCGACAGTGGGTGGTCCGACGATCATCGTTCGACTGTAGAACT2860.11679142113924723No Hit
GCTACAGTGAGTTGCGTGGCGAGCGTGCCCTGGCTACCGCACT2760.11270780501549732No Hit
TCGACCGTGGAATGGATCTCACTCTGTCTTCCAGGATGAGTGC2760.11270780501549732No Hit
GCTACAGTAAACTTGCAGCCATTACACCCATATCGGTACTTGA2750.11229944340312233No Hit
CCGACAATACGGTATCCGCTACGACCTTCACGGACAGGTCACT2740.11189108179074733No Hit
TCGTCAGTAACTAGCTCAGATCCAATAGATCCGCCTGCTCCGG2670.10903255050412242No Hit
TCGACAGTGAAAAAATGCCTAAAAATTTTCCAAAATTAATAAT2640.10780746566699743No Hit
GCGACAGTTCCCTTCAAGCGCAGCAAAACACGGCTGGCAACGC2620.10699074244224745No Hit
ACGACAATTCAGCGATCTGACTTTTTTTTTGCTGAGATCGTCG2620.10699074244224745No Hit
GCTACAGTCTACTTCTACTTGCTACAGTTTTTTTTTTTTTTTT2600.10617401921749747No Hit
GCTACAGTCAGTTAGGTCTGACATGCACACATATGTTTATTGC2500.10209040309374756No Hit
TCGACAGTGAAGGGTCCGACGATCTAGGATCGACGGACTGTAG2490.10168204148137258No Hit
TCGACAGTTTAAACAGTCCGACGATCATGCGTCGACAGTAGAA2480.10127367986899759No Hit
GCTACAGTGGCGTGTGACCGACAGATAACCACAATGAAACTAG2470.1008653182566226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGC200.001835172237.0161413
GGTCCAC251.2276326E-437.0161414
TCCAAGC308.260506E-637.0161413
TATACGA403.797686E-837.0161413
TGGTCCA308.260506E-637.0161413
TGTCCGG308.260506E-637.0161413
CCCGAGC308.260506E-637.0161413
GTGCAAC1150.037.00858312
GCATAAT200.001837014937.0085810
GTTGATA200.001837014937.0085812
TGATATG452.5956979E-937.008588
TCTTGTC200.001837014937.0085810
TCGCATA501.7826096E-1037.008588
TACCCTT200.001837014937.008588
TGCCCTA251.2291102E-437.008588
TCGCAGG200.001837014937.008588
CGTATGG251.2291102E-437.008589
TCCGCAA1450.037.008588
ATAGGTC200.001837014937.0085811
GTTTCTA200.001837014937.0085812
Produced by FastQC (version 0.11.5)