##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099095_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 244881 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.42003258725667 37.0 35.0 37.0 33.0 37.0 2 35.84073897117376 37.0 37.0 37.0 35.0 37.0 3 35.70060560027115 37.0 37.0 37.0 35.0 37.0 4 36.31390757143266 37.0 35.0 39.0 32.0 39.0 5 37.433063406307554 39.0 37.0 39.0 35.0 39.0 6 36.6944230054598 39.0 37.0 39.0 33.0 39.0 7 36.99459737586828 39.0 37.0 39.0 34.0 39.0 8 36.83541801936451 39.0 37.0 39.0 33.0 39.0 9 31.75609785977679 33.0 31.0 34.0 30.0 34.0 10 32.01031521432859 34.0 31.0 34.0 30.0 34.0 11 32.024616037993965 34.0 31.0 34.0 30.0 34.0 12 35.49049946708809 37.0 35.0 37.0 33.0 37.0 13 35.21614988504621 37.0 35.0 37.0 33.0 37.0 14 31.647877948881295 31.0 31.0 34.0 30.0 34.0 15 31.46474001657948 31.0 31.0 34.0 28.0 34.0 16 31.51665911197684 31.0 31.0 34.0 28.0 34.0 17 35.00237666458402 37.0 35.0 37.0 32.0 37.0 18 34.78256377587481 37.0 35.0 37.0 30.0 37.0 19 34.69223010360134 36.0 35.0 37.0 30.0 37.0 20 34.40444134089619 36.0 35.0 37.0 30.0 37.0 21 34.50277481715609 36.0 35.0 37.0 30.0 37.0 22 36.23183505457753 39.0 35.0 39.0 30.0 39.0 23 36.49208799376024 39.0 35.0 39.0 32.0 39.0 24 36.8972643855587 39.0 37.0 39.0 33.0 39.0 25 37.061805530032956 39.0 37.0 39.0 33.0 39.0 26 37.1149782955803 39.0 37.0 39.0 33.0 39.0 27 38.53223402387282 40.0 38.0 41.0 34.0 41.0 28 38.64780852740719 40.0 38.0 41.0 35.0 41.0 29 38.66288115451995 40.0 38.0 41.0 35.0 41.0 30 38.67426627627297 40.0 38.0 41.0 35.0 41.0 31 38.75299431152274 40.0 38.0 41.0 35.0 41.0 32 38.86947537783658 40.0 38.0 41.0 35.0 41.0 33 38.934898991755176 40.0 38.0 41.0 36.0 41.0 34 38.983212254115266 40.0 38.0 41.0 36.0 41.0 35 39.02909168126559 40.0 38.0 41.0 36.0 41.0 36 38.99822771060229 40.0 38.0 41.0 36.0 41.0 37 38.92851629975376 40.0 38.0 41.0 35.0 41.0 38 38.78970602047525 40.0 38.0 41.0 35.0 41.0 39 38.758629701773515 40.0 38.0 41.0 35.0 41.0 40 38.695076384039595 40.0 38.0 41.0 35.0 41.0 41 38.65175738419886 40.0 38.0 41.0 35.0 41.0 42 38.64312461971325 40.0 38.0 41.0 35.0 41.0 43 38.60893250190909 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4300087192895674 1101 2 0.24863766178297197 1101 3 0.4600400646496752 1101 4 0.8646903470029343 1101 5 0.5011987815414969 1101 6 0.48076037841328656 1101 7 0.7228911121799584 1101 8 0.796051886184344 1101 9 0.5846223205559866 1101 10 0.5092778632789035 1101 11 0.45954514323394946 1101 12 0.5118716737488853 1101 13 0.3966662198603359 1101 14 0.006791618257409482 1101 15 0.2566142672403764 1101 16 -0.19454901842529182 1101 17 -0.2666581052631045 1101 18 -0.13010871874490704 1101 19 -0.46163271393785266 1101 20 -0.14536273033353098 1101 21 -0.44819868797722506 1101 22 -0.47884253132425414 1101 23 -0.24027729720312863 1101 24 -0.09292437964310096 1101 25 -0.02097991090064255 1101 26 -0.3464911699606503 1101 27 -0.2503560599232202 1101 28 -0.22725543707596074 1101 29 -0.023357445131189536 1101 30 0.25983146951850244 1101 31 0.029586636601855787 1101 32 -0.11343793179754158 1101 33 -0.25376378145249134 1101 34 0.05573398068647606 1101 35 -0.047304683332690445 1101 36 0.14785424606630926 1101 37 0.16259416669888793 1101 38 0.19437484080913947 1101 39 -0.007637951528906228 1101 40 0.18957427224374612 1101 41 0.21902482511739407 1101 42 -0.023583020989661918 1101 43 -0.030869356790901747 1102 1 0.3293724007659762 1102 2 0.10047532204038845 1102 3 0.3238189047031952 1102 4 0.49637310484385466 1102 5 0.312250497340159 1102 6 0.6010453084056806 1102 7 0.3914150165248884 1102 8 0.7200203026746053 1102 9 0.32197918029580563 1102 10 0.4768212215080183 1102 11 0.5348983219998793 1102 12 0.2254152706759598 1102 13 0.21126071598777685 1102 14 0.050209521861120265 1102 15 0.16229716327485022 1102 16 -0.15282712668109966 1102 17 -0.41624006974736005 1102 18 -0.20636643716925107 1102 19 -0.2917488593426043 1102 20 -0.5043079365874163 1102 21 -0.2813174025258647 1102 22 -0.31136450095936397 1102 23 -0.3673776822606385 1102 24 -0.22053984978386865 1102 25 -0.35058411198482986 1102 26 -0.3664978283775824 1102 27 -0.534940568425263 1102 28 -0.4311788778443173 1102 29 -0.326598084087955 1102 30 -0.12370590869842601 1102 31 -0.13269029072717586 1102 32 -0.040999380819819464 1102 33 0.1256843237253662 1102 34 0.1851082779408202 1102 35 0.02511502306881397 1102 36 -0.13285456126045858 1102 37 0.01700097084720653 1102 38 0.07756987589145581 1102 39 -0.22205782125006834 1102 40 -0.320567250523645 1102 41 -0.13385870912006226 1102 42 -0.28662189735040045 1102 43 -0.16871743181744137 1103 1 0.30477277451191753 1103 2 0.2578950718984814 1103 3 0.2910810562297499 1103 4 0.660196283470178 1103 5 0.5054248504599173 1103 6 0.6345931523508099 1103 7 0.45277057902528384 1103 8 0.5472871738598144 1103 9 0.31602858364166053 1103 10 0.2919445385351409 1103 11 0.3615274014751044 1103 12 0.49346637054776465 1103 13 0.3749818479405036 1103 14 -0.15718758639126662 1103 15 -0.34125852550830515 1103 16 -0.6960725935765204 1103 17 -0.5595646228148183 1103 18 -0.5370933283811183 1103 19 -1.0439476462627724 1103 20 -0.9218191457767233 1103 21 -0.41719892633781797 1103 22 -0.4628272762462089 1103 23 -0.5434719733870423 1103 24 -0.6306207855518622 1103 25 -0.6443637294373659 1103 26 -0.7722354026005931 1103 27 -0.6450425513196905 1103 28 -0.6296596579911409 1103 29 -0.6246381501631717 1103 30 -0.4544965790812938 1103 31 -0.36015870409273987 1103 32 -0.16860874615328925 1103 33 -0.3707408537401733 1103 34 -0.296743358951602 1103 35 -0.200911981032192 1103 36 -0.22849780608956394 1103 37 -0.14028484275178954 1103 38 0.05820651957635903 1103 39 -0.10361352278906111 1103 40 -0.09853282252714024 1103 41 -0.04885383001347776 1103 42 -0.08568561912014161 1103 43 -0.06561202398153654 1104 1 0.4580519581539093 1104 2 0.38405656992569703 1104 3 0.45462254885913467 1104 4 0.4722938557304559 1104 5 0.48626896874957737 1104 6 0.3270130523623749 1104 7 0.41452307552312107 1104 8 0.3849409157148713 1104 9 0.5888183637442168 1104 10 0.39243419911424837 1104 11 0.32777166627737486 1104 12 0.36622530588943647 1104 13 0.23187500939997108 1104 14 -0.07272434949064888 1104 15 -0.024052289725812415 1104 16 -0.3410750997133434 1104 17 -0.4544611000860854 1104 18 -0.6442917244535522 1104 19 -0.6380627229248574 1104 20 -0.5353985517580355 1104 21 -0.8025206757498395 1104 22 -0.42283832895219575 1104 23 -0.6253069796202553 1104 24 -0.5798425979386082 1104 25 -0.25978096908830395 1104 26 -0.376028022991548 1104 27 -0.42442539906223686 1104 28 -0.3816356504855847 1104 29 -0.3189601437628866 1104 30 -0.08928818416553952 1104 31 -0.04269542863617204 1104 32 -0.09770435206006312 1104 33 0.010783134487404311 1104 34 0.13103491862554506 1104 35 -0.014616051255430307 1104 36 -0.07109313567868725 1104 37 0.04928834286940997 1104 38 -0.11708168615819403 1104 39 -0.14267712725641246 1104 40 -0.023721425387989825 1104 41 0.03089272886788308 1104 42 0.012548518318972413 1104 43 0.01543901124575342 1105 1 0.06621778729964234 1105 2 -0.1921368974109825 1105 3 0.03886878001491567 1105 4 0.24838682055457184 1105 5 -0.07836670964238834 1105 6 0.06932461015131963 1105 7 -0.04508747724069906 1105 8 0.10138563933296041 1105 9 0.2771475094728615 1105 10 -0.02950047425258262 1105 11 0.26370131451858114 1105 12 -0.0678827343115671 1105 13 -0.22773554336384905 1105 14 -0.09306354547054951 1105 15 -0.10367540530370434 1105 16 -0.31442937107012625 1105 17 -0.5297500448097026 1105 18 -0.5249449907852082 1105 19 -0.7907762907640503 1105 20 -0.8496196572856718 1105 21 -0.5533498214784842 1105 22 -0.5566699872436516 1105 23 -0.5620908992182407 1105 24 -0.42137526125518576 1105 25 -0.5763010695908193 1105 26 -0.45215867625788064 1105 27 -0.3849530372531902 1105 28 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1107 14 -0.10403961599951472 1107 15 -0.04313384359085859 1107 16 -0.48170267081824036 1107 17 -0.5588495410313641 1107 18 -0.2787233283166941 1107 19 -0.28279140411417814 1107 20 -0.41992192428818953 1107 21 -0.20134730258679667 1107 22 -0.08337653636203868 1107 23 -0.5299498412837309 1107 24 -0.6244105257388171 1107 25 -0.320728021731874 1107 26 -0.25832996089263816 1107 27 -0.2906583269257368 1107 28 -0.39350228846135593 1107 29 -0.3734833897155738 1107 30 -0.0777000166275954 1107 31 -0.22230253216388718 1107 32 -0.17641022398898087 1107 33 -0.08751693006710326 1107 34 -0.047540578507479836 1107 35 0.19450891868242337 1107 36 -0.0057981468807923875 1107 37 -0.06914386856559673 1107 38 0.08354360655221171 1107 39 -0.06809135203268823 1107 40 0.05607301279790278 1107 41 0.057815757107320565 1107 42 -0.22509435600225913 1107 43 -0.06865273210576817 1108 1 0.09371778729963864 1108 2 -0.35713689741098165 1108 3 -0.13113121998508603 1108 4 -0.039113179445422475 1108 5 -0.09586670964238664 1108 6 -0.1656753898486798 1108 7 -0.1300874772406999 1108 8 -0.16361436066704016 1108 9 0.24964750947286163 1108 10 0.1279995257474198 1108 11 0.08620131451857915 1108 12 -0.09038273431156796 1108 13 -0.007735543363850184 1108 14 0.08943645452945148 1108 15 -0.0786754053037022 1108 16 -0.10442937107012895 1108 17 0.12274995519029375 1108 18 0.18255500921479495 1108 19 0.0942237092359477 1108 20 0.02288034271433048 1108 21 0.05915017852152005 1108 22 0.043330012756349845 1108 23 0.21540910078175557 1108 24 0.023624738744807416 1108 25 0.06619893040918612 1108 26 -0.1796586762578798 1108 27 -0.22245303725319587 1108 28 -0.17558037410367433 1108 29 -0.22073150054680468 1108 30 -0.03479069672835067 1108 31 -0.19367532813365784 1108 32 -0.4414731962811729 1108 33 -0.11159123737188992 1108 34 -0.22199271956541367 1108 35 -0.08722911658205845 1108 36 0.07929631155749206 1108 37 -0.07812371743209923 1108 38 0.02467710781165522 1108 39 0.08177010892449488 1108 40 0.05538661481301688 1108 41 0.04745051781260656 1108 42 -0.025730376153390466 1108 43 -0.020138878201485966 1109 1 0.44580962403433233 1109 2 0.3724039189155448 1109 3 0.2783585759332823 1109 4 0.26996845320763896 1109 5 0.39331696382700443 1109 6 0.2332021611717252 1109 7 0.44394313500419713 1109 8 0.3065387005574465 1109 9 0.29036179518714533 1109 10 0.4366219747270108 1109 11 0.35553804921245913 1109 12 0.27420910242312146 1109 13 0.2689481301055423 1109 14 0.07372216881516636 1109 15 -0.04816520122206924 1109 16 0.10286654729721789 1109 17 -0.060719432564809495 1109 18 -0.2851490724178589 1109 19 0.0717747296441118 1109 20 -0.1168645552448595 1109 21 -0.30763553576419866 1109 22 -0.5259046811212045 1109 23 -0.3137235522794697 1109 24 -0.12106913880621306 1109 25 -0.11992351857040973 1109 26 -0.015423982380326606 1109 27 -0.02740201684503063 1109 28 -0.2037436394097938 1109 29 -0.08394578626108995 1109 30 -0.08009681917732792 1109 31 -0.21571614446018827 1109 32 -0.3310650330158609 1109 33 -0.06613205369841779 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0.7559223888753621 2216 25 0.5132224291036991 2216 26 0.37042618013898476 2216 27 0.38388508285124345 2216 28 0.3633621846430657 2216 29 0.33982332974039764 2216 30 0.10655395079123053 2216 31 0.12816540293683687 2216 32 0.010145602674441534 2216 33 0.07246228743228755 2216 34 0.05944983918132607 2216 35 0.0698204917730294 2216 36 0.10340205568282101 2216 37 0.1478162303485817 2216 38 0.19029068483515488 2216 39 0.23371527863729114 2216 40 0.18300410828560132 2216 41 0.052633285436627375 2216 42 0.20272262645757877 2216 43 0.34764180064968997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 6.0 26 41.0 27 213.0 28 537.0 29 1101.0 30 2143.0 31 3932.0 32 7156.0 33 11424.0 34 18558.0 35 31863.0 36 49106.0 37 54950.0 38 63851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 59.15077119090497 2.0789689686010755 36.46791706992376 2.3023427705701955 2 1.2246764755125958 0.9331062842768528 1.5656584218457128 96.27655881836485 3 37.16213181096124 1.8833637562734553 59.316157643916846 1.638346788848461 4 0.7959000326690623 95.07228030055538 2.581264292714799 1.5505553740607645 5 0.8257071802222303 1.435391067498091 0.32464748183811726 97.41425427044156 6 1.455406729826854 96.69021561581182 0.9175922901012742 0.9367853642600458 7 91.06749809090945 3.836965709875409 1.2965481192905943 3.7989880799245346 8 2.1962046251791714 0.6840168737774475 94.4470080897757 2.6727704112676975 9 30.390163023947203 20.838233852788395 21.16861053938384 27.60299258388056 10 28.053740607644563 19.448709572035284 26.18016987912447 26.31737994119569 11 27.265450476388835 19.18460526906883 25.329265752686197 28.22067850185614 12 27.383806167328924 22.81813689657003 24.947421295294255 24.850635640806793 13 9.61858869650441 12.479581836001307 66.09523031688991 11.806599150604377 14 45.67506412455685 21.027544968877127 14.86382721495205 18.433563691613976 15 29.250722814812395 23.81205181397932 26.92954801613878 20.007677355069507 16 21.992395996259255 24.511681660956178 22.435078061509937 31.06084428127463 17 18.225587121908195 29.884311155214167 22.335338388850097 29.55476333402755 18 32.664837184230514 27.25724646558694 17.148539272617384 22.929377077565157 19 21.628830271294717 31.506866100822624 13.398086804506057 33.466216823376605 20 22.867387611082073 25.127009278619965 25.44759539989545 26.55800771040251 21 27.677933363552093 25.626732984592515 22.23937340994197 24.45596024191342 22 22.35779827753072 27.62117109943197 24.59766172140754 25.42336890162977 23 23.56981554305969 25.295143355344024 26.1723040987255 24.96273700287078 24 23.22597506543995 26.889795451668363 23.611468427521938 26.272761055369752 25 22.512975690233215 26.13841008489838 26.418137789375244 24.93047643549316 26 21.943400849395623 27.408526625285855 26.03969291081346 24.608379614505065 27 22.747783617348833 29.083105671734433 23.75071973734181 24.41839097357492 28 22.887851650393458 27.13603750393048 26.3585169939685 23.617593851707564 29 22.014774523135728 27.298565425655724 28.101404355584958 22.585255695623587 30 24.484953916392044 24.1117114026813 29.71034910834242 21.692985572584234 31 22.586889142073087 24.76386489764416 27.176465303555602 25.472780656727146 32 24.0034792977699 25.51485031260592 27.927490127696764 22.554180261927417 33 22.446821109028466 29.61152559814767 25.508308117003768 22.433345175820094 34 21.34663524953344 28.590271850765898 26.372423706595555 23.69066919310511 35 23.066368284356166 28.27145167350006 25.637057122788676 23.025122919355102 36 25.27676708278715 25.92075334550251 27.122969932334478 21.67950963937586 37 23.16488004083716 26.81082184788157 29.158550280755485 20.865747830525777 38 22.626184253511923 24.89260045736687 28.58624632473048 23.894968964390724 39 22.754725764759208 25.941988149346006 29.00633368860794 22.296952397286844 40 22.135415603138107 26.70494688823455 30.49934452607806 20.660292982549283 41 20.699315549602236 27.81008543378473 29.819331231520657 21.671267785092375 42 22.327987879827344 27.340218310117976 29.3857016265043 20.946092183550377 43 23.132429750446143 24.992955647120798 30.42670401875227 21.447910583680788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.5 7 5.0 8 16.5 9 28.0 10 86.0 11 144.0 12 144.0 13 298.5 14 453.0 15 764.0 16 1075.0 17 1213.5 18 1352.0 19 1352.0 20 1327.0 21 1302.0 22 1854.0 23 2406.0 24 2614.0 25 2822.0 26 2822.0 27 3311.5 28 3801.0 29 4396.0 30 4991.0 31 5543.0 32 6095.0 33 6095.0 34 6547.0 35 6999.0 36 8633.5 37 10268.0 38 11244.0 39 12220.0 40 12220.0 41 12513.5 42 12807.0 43 14535.5 44 16264.0 45 18107.0 46 19950.0 47 19950.0 48 22714.5 49 25479.0 50 24696.0 51 23913.0 52 25212.0 53 26511.0 54 26511.0 55 23605.5 56 20700.0 57 17528.0 58 14356.0 59 13208.0 60 12060.0 61 12060.0 62 8608.5 63 5157.0 64 4233.5 65 3310.0 66 3341.0 67 3372.0 68 3372.0 69 2334.5 70 1297.0 71 2385.0 72 3473.0 73 2448.5 74 1424.0 75 1424.0 76 937.5 77 451.0 78 325.0 79 199.0 80 183.5 81 168.0 82 168.0 83 97.0 84 26.0 85 14.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 4.0836161237499033E-4 5 0.0 6 4.0836161237499033E-4 7 0.0 8 0.0016334464494999613 9 0.0036752545113749127 10 4.0836161237499033E-4 11 0.007350509022749825 12 0.0040836161237499035 13 4.0836161237499033E-4 14 0.018376272556874563 15 0.0020418080618749518 16 0.0040836161237499035 17 0.0 18 0.0012250848371249709 19 0.022459888680624464 20 0.006942147410374835 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 4.0836161237499033E-4 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0016334464494999613 33 0.0 34 0.0016334464494999613 35 0.0020418080618749518 36 0.0 37 0.0024501696742499418 38 4.0836161237499033E-4 39 0.0 40 0.008167232247499807 41 0.005308700960874874 42 0.0 43 0.0016334464494999613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 244881.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.547376072459684 #Duplication Level Percentage of deduplicated Percentage of total 1 53.39175235033796 9.902769100093515 2 12.783196459631432 4.7418950428983875 3 6.724058213522975 3.741409092579661 4 4.469495145203549 3.315896292484921 5 3.188093088795438 2.95653807359493 6 2.4174904775534465 2.6902863023264363 7 2.0806270503533764 2.7013120658605607 8 1.7195446839428432 2.5514433541189394 9 1.4971707875558686 2.4991730677349406 >10 10.62330742640745 34.953712211237296 >50 0.6671216891609238 8.575185498262421 >100 0.3963099143530241 14.35146050530666 >500 0.03082410444967965 3.667495640739788 >1k 0.011008608732028446 3.351423752761545 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTACAGTTGAATGCGAAATGACACAAAATAGAACCTGCAAAT 2280 0.9310644762149779 No Hit GCTACAGTCTGCTGTCCGACGATCGGCTTGCTACAGTTTTTTT 2041 0.8334660508573553 No Hit CCGACAGTCTCCCCACGGATGGCCGCGACGCTGGCGGCGATCT 1642 0.670529767519734 No Hit TCGACAAGGCGACGGATGCGCCCGGCATCGGGATCGAGGTAGC 1148 0.46879913100648885 No Hit GCTACAGTGGTGTGCAACATATACCAGCTTCCCCACTGGGTGT 1096 0.44756432716298933 No Hit GCTACAGTCTGCTGGTCCGACGATCGGCTTGCTACAGTTTTTT 985 0.40223618818936546 No Hit TCGACAGTATTGCGGCAAGTTACTTAACCTCTCTGAGTCTCTG 864 0.35282443309199163 No Hit GCTACAGTATAATGTCCGACGATCATCCTGCTACAGTTTTTTT 758 0.30953810218024264 No Hit TCGACAGTTCTGAGTAGAAGATATGCAGCTTGGTCTTCATGTG 695 0.28381132060061826 No Hit TCGACGATGCTCTGGTGGGGCCGTCCTCGCCCACGCCCACCGA 690 0.28176951253874327 No Hit TCGACAACCGCTAGAAACCTATCGGAACAGATTGTGAAATCTG 655 0.26747685610561867 No Hit TCGAAAGTTTTTCTATCCATCAGGACCATCTAATGTGAAGCGT 621 0.25359256128486896 No Hit GCTACAGTCTACTGTCTACTTGCTACAGTTTTTTTTTTTTTTT 617 0.25195911483536904 No Hit TCGACAGTACCAGGAAAGAAGCAAATGAAAAACAATCTAAATT 537 0.2192901858453698 No Hit TCGACAGTCGCTAGTCCGACGATCATACGTCGGACTGTAGAAC 521 0.21275640004736995 No Hit GCTACAGTTTGTTCAGTCCGACGATCAAAAACTGCTAGTTTTT 518 0.21153131521024499 No Hit GCTACAGTAGCTTGTCCACTGTAGGAGGTGGAATATGGCAAGA 512 0.20908114553599505 No Hit GCTACAGTGATGTCCTAGGTTTTGCATATGACCTGCAGCCTAA 505 0.2062226142493701 No Hit TCGACAGCGACATCCCAATCCGGCGGCCAGGACCGCAAACGCA 503 0.20540589102462015 No Hit TTGCCAGTGGGGCGGCGCCCTTTTCCACCGTCAACATCTTCAC 479 0.19560521232762038 No Hit GCTACAGTGTGATGATCTGCGTGCTACAGTTTTTTTTTTTTTT 474 0.1935634042657454 No Hit GCTACAGTACCCTGGCAGCTTTGGATCGCCGAAATCCTGGTCT 471 0.19233831942862045 No Hit GCTACAGTATCCTGCGTGGTGGACGTCCAAACCGTGGCAGCAG 469 0.19152159620387046 No Hit TCGACAGTCGCATATTATCAGCTTAGCCCTGACCAGAATCGGA 467 0.19070487297912048 No Hit TCGACAGTCCGCAATTGCTGAAAAAATTCCAAATACGTCCATG 463 0.1890714265296205 No Hit GCTACAGTCTTTTGTTAGAAGAATATTGTGAAAATGACCACAT 462 0.18866306491724552 No Hit TTGCCAGTGGGGCGCGCCCTTTTCCACCGTCAACATCTTCACG 461 0.18825470330487054 No Hit TCGACAAGGCGACGGGATGCGCCCGGCATCGGGATCGAGGTAG 458 0.18702961846774557 No Hit GCTACAGTGATGTGGTTGCGGTGAGCCGAGATCGTGCCATTGC 455 0.18580453363062058 No Hit TCGACAGTTCATGGGAGAGAGGTAAGAGATGAACTACTGGAGG 420 0.17151187719749592 No Hit TCGACAGTCCAGCGGTCGCCAAGCGAAAGATGAGCGACCCTCA 400 0.16334464494999612 No Hit GCTACAGTTCAGTAGGAGCAGTGGAGCCAGCTCCCTCACCCCT 383 0.1564024975396213 No Hit GCTACAGTACTATCCTGAGTAGCTGGGACTACAGGCGCCTGCC 371 0.1515021581911214 No Hit GCTACAGTGTCATTCCGACGATCATCGGACTGTAGAACTCTGA 368 0.15027707335399643 Illumina DpnII expression Sequencing Primer (95% over 24bp) TCGTCACTCATGACATTCCGACGATCCATGATCAGCAAGTCAG 366 0.14946035012924644 No Hit GCTACAGTATAGTTGATCGACTGGTGATAAAGACGATAATAGC 351 0.1433349259436216 No Hit TCGACACTAGCACCCGAGCCCTCCATGGAGCCAGGCATCTGTT 340 0.1388429482074967 No Hit ACTACAGTGTGGTGCCGGGCCTCGCCCGGCGCATGGGCCCGCA 339 0.1384345865951217 No Hit TCGACAGTGATTCGTCCGACGATCTAGGATCGACAGACTGTAG 330 0.1347593320837468 No Hit GCTACAGTGCTTTGTGCACGCAGGTGCGAGTGGAATTCATGGA 326 0.13312588563424685 No Hit GCTACAGTATAGTGGCCAATGGGTTATCTGGACCAAAGGTGGG 322 0.13149243918474687 No Hit TCGACAGTCCAGCGTCGCCAAGCGAAAGATGAGCGACCCTCAT 319 0.1302673543476219 No Hit GCTACAGTTCTTTGCCGAAGTGGGAGGATCACTTGAGCTCAGG 299 0.1221001221001221 No Hit TCAACATTTCGAAACAGTCCGACGATCTCGAAAAAGAAAGCAG 297 0.12128339887537212 No Hit GCTACAGTCCGCTGTGAGCTGTAGCCTGAGGGACGTGAGGGAC 291 0.11883322920112217 No Hit TCGACAGTATTGCGGGCAAGTTACTTAACCTCTCTGAGTCTCT 289 0.11801650597637219 No Hit TCGACAGTGGGTGGTCCGACGATCATCGTTCGACTGTAGAACT 286 0.11679142113924723 No Hit GCTACAGTGAGTTGCGTGGCGAGCGTGCCCTGGCTACCGCACT 276 0.11270780501549732 No Hit TCGACCGTGGAATGGATCTCACTCTGTCTTCCAGGATGAGTGC 276 0.11270780501549732 No Hit GCTACAGTAAACTTGCAGCCATTACACCCATATCGGTACTTGA 275 0.11229944340312233 No Hit CCGACAATACGGTATCCGCTACGACCTTCACGGACAGGTCACT 274 0.11189108179074733 No Hit TCGTCAGTAACTAGCTCAGATCCAATAGATCCGCCTGCTCCGG 267 0.10903255050412242 No Hit TCGACAGTGAAAAAATGCCTAAAAATTTTCCAAAATTAATAAT 264 0.10780746566699743 No Hit GCGACAGTTCCCTTCAAGCGCAGCAAAACACGGCTGGCAACGC 262 0.10699074244224745 No Hit ACGACAATTCAGCGATCTGACTTTTTTTTTGCTGAGATCGTCG 262 0.10699074244224745 No Hit GCTACAGTCTACTTCTACTTGCTACAGTTTTTTTTTTTTTTTT 260 0.10617401921749747 No Hit GCTACAGTCAGTTAGGTCTGACATGCACACATATGTTTATTGC 250 0.10209040309374756 No Hit TCGACAGTGAAGGGTCCGACGATCTAGGATCGACGGACTGTAG 249 0.10168204148137258 No Hit TCGACAGTTTAAACAGTCCGACGATCATGCGTCGACAGTAGAA 248 0.10127367986899759 No Hit GCTACAGTGGCGTGTGACCGACAGATAACCACAATGAAACTAG 247 0.1008653182566226 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0016334464494999611 0.0 0.0 13 0.0 0.0 0.0016334464494999611 0.0 0.0 14 0.0 0.0 0.0024501696742499418 0.0 0.0 15 0.0 0.0 0.0036752545113749127 0.0 0.0 16 0.0 0.0 0.011434125146499729 0.0 0.0 17 0.0 0.0 0.01633446449499961 0.0 0.0 18 0.0 0.0 0.02491005835487441 0.0 0.0 19 0.0 0.0 0.02776858964149934 0.0 0.0 20 0.0 0.0 0.06084588024387356 0.0 0.0 21 0.0 0.0 0.23929990485174432 0.0 0.0 22 0.0 0.0 0.25971798547049385 0.0 0.0 23 0.0 0.0 0.4549148361857392 0.0 0.0 24 0.0 0.0 0.6819638926662338 0.0 0.0 25 0.0 0.0 0.7885462734961063 0.0 0.0 26 0.0 0.0 1.0670488931358497 0.0 0.0 27 0.0 0.0 1.1981329707082216 0.0 0.0 28 0.0 0.0 1.351268575348843 0.0 0.0 29 0.0 0.0 1.4590760410158403 0.0 0.0 30 0.0 0.0 1.6514143604444609 0.0 0.0 31 0.0 0.0 1.8829553946610802 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATAGC 20 0.0018351722 37.01614 13 GGTCCAC 25 1.2276326E-4 37.01614 14 TCCAAGC 30 8.260506E-6 37.01614 13 TATACGA 40 3.797686E-8 37.01614 13 TGGTCCA 30 8.260506E-6 37.01614 13 TGTCCGG 30 8.260506E-6 37.01614 13 CCCGAGC 30 8.260506E-6 37.01614 13 GTGCAAC 115 0.0 37.008583 12 GCATAAT 20 0.0018370149 37.00858 10 GTTGATA 20 0.0018370149 37.00858 12 TGATATG 45 2.5956979E-9 37.00858 8 TCTTGTC 20 0.0018370149 37.00858 10 TCGCATA 50 1.7826096E-10 37.00858 8 TACCCTT 20 0.0018370149 37.00858 8 TGCCCTA 25 1.2291102E-4 37.00858 8 TCGCAGG 20 0.0018370149 37.00858 8 CGTATGG 25 1.2291102E-4 37.00858 9 TCCGCAA 145 0.0 37.00858 8 ATAGGTC 20 0.0018370149 37.00858 11 GTTTCTA 20 0.0018370149 37.00858 12 >>END_MODULE