FastQCFastQC Report
Thu 26 May 2016
SRR2099094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308646
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTCAGTCGTATGCAGGCTCTGGGCTCCAGCAGTGGTCTCT17600.5702325641673633No Hit
CATGTCAGGTTTAAATCGACTGTTACAAACTTACACTTAGATA9300.3013160708384363No Hit
CATGTCAGCTCGAGTCCACATGTCGTGATTGTGCAGAATAAAC8960.2903002144852031No Hit
CATGTCAGTGCGGAGGGGAATTCAGGCTGCTTCTTAATTTATT8810.28544027785877674No Hit
CATGTCAGTAATTGGATTACAGGCATGCACCACCACACCAGGC8580.2779883750315896No Hit
CATGTCAGTGAAAAAAAACCAGGAATCCAGACACAGTGGTGCC7760.25142072147379196No Hit
CATGTCAGGGTACAAAAGATTCCACAACAGTGGAAAATTATAG7420.24040486512055884No Hit
CATGTCCACTCTCGCATTTGAGTCAGTGGTTTTGAGGTAGATG7200.2332769580684668No Hit
CATGTCAGTATATGTCTGGACCGCGTCGATGTCACCCGCGCGC6880.2229090932654238No Hit
CATGGCAAACATCGCGCAAGCCCTGTCGATCGCTAACCAGTCG6800.22031712706466308No Hit
GATGTCGGGTACATACAGTCCGACGATCGTACTCACTACTTGT6300.20411733830990844No Hit
CATGTCAGTTAGTGAAAGTCCGAGCCTTAGGACCCAGTTTCAG6050.19601744393253112No Hit
CATGTCAGTATATTCTGGACCGCGTCGATGTCACCCGCGCGCT6010.19472146083215075No Hit
CATGTCAGAAAGGATTGCACTTTCCGTTCTAAGTGCAATTTCC5930.19212949463139No Hit
CATGGCAGTCTTGGGTTCTGGAGACTGGGAAGTCCAAGATCAA5530.1791696636275863No Hit
CATGTCAGTCTTTGTCTGACGATCTTTTTCATGTCAGACTGTA5390.174633722776255No Hit
CATGTCAGGAATTACGATCTATTTCATGTCAGTTTTTTTTTTT5140.16653382839887768No Hit
CATGTCAGATGGCGAAATTGCACTTTCCGTTCTAAGTGCAATT5130.16620983262378258No Hit
CATAACAGAGTCAGCTCCTTAGGTCTGTTCCAGGCTTTCTATT5080.16458985374830712No Hit
CATGTCAGTCGTAGTGCAGGCTCTGGGCTCCAGCAGTGGTCTC5030.16296987487283165No Hit
CATGTCAATAGACCTTTTTTGATGTGGTTGTCGTACTTGATGT4920.15940592134678563No Hit
CATGTCAGGTCGGACGGAAATTGCACTTTCCGTTCTAAGTGCA4860.15746194669621508No Hit
CATGTCAGGGCTAGTCCGACGATCTTTGGCATGAGTAAGATCG4670.1513060269694083No Hit
CATGCCTGGTACGCGATCGAGCGGCTGGAGCGCGACGCGATCG4640.15033403964412304No Hit
CATGTCAGTACTCGCGGGGCGCGGGACATGTGGCGTACGGAAG4550.1474180776682672No Hit
CATGTCACTTTGGTGGCCCTCAGGTCATGCTGGGTTGGGTCTT4520.14644609034298192No Hit
CATGTCACTTTGGCATGGCCCTCAGGTCATGCTGGGTTGGGTC4520.14644609034298192No Hit
CATGTCAGAGAGGCGGTAAACTTAATAAGCAACCTCTTATTAC4370.14158615371655553No Hit
CATGTCAGTTTGAAAGACACATCAGGACGAGAAAAGGCATGGG4360.14126215794146044No Hit
AATGTCGGGTACCGTTTTAAGCCAACTTTTTCACTCTCCTCTT4290.1389941875157948No Hit
CATGGCAGTCTTGGTTCTGGAGACTGGGAAGTCCAAGATCAAG4240.13737420864031932No Hit
CATGGCAAACATCGGCGCAAGCCCTGTCGATCGCTAACCAGTC4170.13510623821465367No Hit
CATGTCAGAATTCGAAAGGACCGGTGCGCATGCCTACCAAGAC3940.12765433538746654No Hit
CATAACAGAGTCAGGCTCCTTAGGTCTGTTCCAGGCTTTCTAT3860.1250623691867058No Hit
CATGTCAGTTAGACGGAAATTGCAATTTCCGTTCTAAGTGCAA3770.12214640721084997No Hit
CATGTCAGGTCTGCAGTCCGACGATCTGTGACATGTTATAGAT3730.12085042411046959No Hit
CATGTCAGTGTGCCCACCAGCAATGGCCGTGCAAAAACCCGCG3720.12052642833537451No Hit
CATGTCAGTATCAGGTGTCGCGCAGCACCTTCATGACGTCGGC3700.11987843678518433No Hit
CATGTCAGAGTTGGGTGGCTGGACTTCAGGCCTCGGGAGCCCT3530.11437050860856775No Hit
CATGTCAGCTCATGCCGTCGATGCCCTGCTTAACCCGGAGATC3440.1114545466327119No Hit
CATGTCAGGCCTCCAGAGCGCGCCGAAAGCAATCGCACGGCCA3370.10918657620704625No Hit
CATGTCAGTTCTTGGCGGAAACTGGATCCTGGGAGCGAGGAAA3260.10562262268100024No Hit
CATGTCCGCATCTGTCCGACGATCGGTTCCATGTCAGTTTTTT3250.10529862690590513No Hit
CATGTCGATTGGAGGCGCCTGATCAGGTCAGCAAAGCCAGCCT3140.10173467337985913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG308.280076E-637.00794213
CGAACAC200.00183791237.00794234
GGTGTCG308.280076E-637.00794214
TGGCGGA403.810601E-837.00794213
TGACGTC308.280076E-637.00794234
TGCGAGC200.00183791237.00794213
CGCTCAA251.2299788E-437.0079413
GTTTGCG200.001839375637.0019458
TTGGCGG501.7826096E-1037.00194512
TCGTTAG251.2311529E-437.00194512
GTCGACA200.001839375637.0019458
GCGAACA403.816058E-837.00194510
AACTTGG251.2311529E-437.0019459
TATGGCC200.001839375637.00194510
GGCGTAG251.2311529E-437.0019458
GCTATCC200.001839375637.00194510
CGCGATT251.2311529E-437.00194511
AATGGCG200.001839375637.00194511
CGCTGAT251.2311529E-437.00194537
CGCCTAG200.001839375637.00194512
Produced by FastQC (version 0.11.5)