FastQCFastQC Report
Thu 26 May 2016
SRR2099093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861521
Sequences flagged as poor quality0
Sequence length42
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGAATCCTAGGCCATCTTCTGCTACATATGCAGCAG73600.8543030291774664No Hit
ACTGCTAGAGACAGATGGCCTAATCGGCCATCAGTGGAAAGA40650.4718399203269566No Hit
ACTGCTTGTCTATGTACTTCGGAGTGTTACCCTGGACATTAT31640.36725744352139994No Hit
ACTGCTAGATTCCCTTGGTCCTGTGATGGTTCTATGCCCCAG25710.298425691306422No Hit
ACTGCTAGCGTGGGGAGGAGAGGCCCTTCATCCTGTGAAGGC23180.2690590246784466No Hit
ACTGCTAGTAATGTTGCCACCTCATGTTTATAAAAACAGAGC22030.2557105398475487No Hit
ACTGCTAGCGCGGGAGGGAGCACCCTCTCAAAGGCAACAGGG21910.2543176544738898No Hit
ACTGCTAGCACGATGAAGGCTCAATAGATGCCCCAGTGTAGG21160.24561212088852158No Hit
ACTGCTAGGGACGGTAGTAGGGGAGGAGGGTAAATTACAGTC18400.2135757572943666No Hit
ACTGCTAGATTTAAAACACATCAGGAAGTTTCAATGTTGCTG17850.20719169933176326No Hit
ACTGCTAGGTCAGAGCATTTAAGCCTCTGACCCACACTGTGG16240.18850382056850615No Hit
ACTGCTAGCGGAGGGATGGGGGAGGGTATAGGGAACTTTCAG16070.18653056628915604No Hit
ACTGCTAGAGCTGGCTCGATGCCCCAGTGTAGGGGAATGCCA15870.18420909066639118No Hit
ACTGCTAGAAGAAAAGAGAGGCCCATTGGACTTGCAAACTTT15280.17736073757923487No Hit
ACTGCTAGGCACAAAATCACGGAAATGAGAAATACACACTTT14710.17074453205435505No Hit
ACTACTAGAGATCACAGGGCCCCCAATGGAGGAGCTAGAGAA14590.1693516466806961No Hit
ACTGCTAGCAGGCTTTTCTGGCCCTGGCTGGGGCAGCATGAA14440.16761053996362246No Hit
ACTGCTAGACCAGGCCAGCCACAAGCTGTGCTAAGCCCCACT14300.16598550702768708No Hit
ACTGCTAGAGGTAGAGAGACAGACAAATACACACAGACAGAG13690.15890500637825428No Hit
ACTGCTAGAGGCATGCCCCAGTAAAGAGGAATGCCAGGACCA13630.1582085636914248No Hit
ACTGCTAGCGAGGGCAGCAGTTGCTGAAAGAGCAAGTCCTCA13550.15727997344231887No Hit
ACTGCTAGATCCTGTGAAGGCTCTATGCCCCAGTGTAGGGGA12370.14358326726800624No Hit
ACTGCTAGAATGGGGTGAGGAGGCAGGAGTGGGTGAGTGGCC12150.1410296440829649No Hit
ACTGCTAGCGGTATTTAAGGTGGTACCTATGGGTGTGGAACA12080.14021712761499722No Hit
ACTGCTAGTTTGGGCTGCAGCAGGGTCCCTCAGAAGCCCTTC11920.13835994711678531No Hit
ACTGCTAGCGAAACCAGCCAGAGGACAGACTAGAAGGGAGCT11250.13058300378052307No Hit
ACTGCTAGGTAGAGGAGGGGAGGATGCTGCAAATGGCATGTA11210.13011870865597008No Hit
ACTGCTAGACCACCAGCCACAAGCTGTGCTAAGCCCCACTTT11180.12977048731255536No Hit
ACTGCATGTCTGGGAGGAACTAAGTTCTGTGAAGGCTTGATG11160.12953833975027887No Hit
ACTGCTAGGGATAGTACCCAAGGAGCTGGGGGGATCTGCAAC10930.1268686427840993No Hit
ACTGCAAGTGATGGCCACGTTTTAGCCCGGGCACTTCTGGCT10790.12524360984816388No Hit
ACTGCTAGCGACAAGGCAGTACAAAAGCACTAATAAGCCGAT10710.12431501959905794No Hit
ACTGCTATGGTAGCCACACAAGTGTGAGGGAGGATCTGAGAT10690.12408287203678146No Hit
ACTGCTAGAATCGCTAGGCCATCTTCTGCTACATATGCAGCA10630.12338642934995202No Hit
ACTGCTAGAACATCACTATAGTGAGTCGTATTACCGGCCGAC10520.12210961775743134No Hit
ACTGCTAGGCGGGTGGCAGGTAGGGACATCCACTTGGAGACA10500.12187747019515485No Hit
ACTGCTAGGGTAGTGAGTTCAAGGACAGCCTGGTCTACAAAG10370.1203685110403577No Hit
ACTGCTAGCCGTAGGTTTGCCCGGGCACAGTGGCTCATGCCT10320.11978814213466649No Hit
ACTGCTCTTACTATCCGAAGATGGCCTAGTCAGCCATCAGTG10190.11827918297986932No Hit
ACTGCTGTGCGAGGGAGACAGAGGGTGGGGGAGGAGGTATGG9820.11398445307775436No Hit
ACTGCTGTGCGAGGGGAGACAGAGGGTGGGGGAGGAGGTATG9630.11177905123612773No Hit
ACTGCTGTTCACGGTTGGCAGGCCTTGGTGGGCACAACTGGC9420.10934150183222464No Hit
ACTGCTAGGATTAGAAGACAGCCACAAGAACAGAATGCCAAC9330.10829683780198046No Hit
ACTGCTAGAGCCCCTGGCCCTGAGGGTGTTAAATGTTTGCTG9270.10760039511515099No Hit
ACTGCTAGACCCTTAAGATGCCTTCTCTTTCTCTAAGAATAG9200.1067878786471833No Hit
ACTGCTAGCCGGAATTTGAGAAACATCCACTTGAGGACTTAA9140.10609143596035384No Hit
ACTGCAAATGGTGGACCCTTGGTCTAGCAAACTCTATATGCC8810.10226100118279184No Hit
ACTGCTAGTCCAATTGGTGGGAAGAAATTCAAACATTTGCAT8770.10179670605823886No Hit
ACTGCTAGCTCGGAAACAAAAAGAGGGTGTCCAGGTGAATAG8700.10098418959027117No Hit
ACTGCTAGTACGGTCGGCCATCAATGGGAGGAGAGGCCCTTG8650.10040382068457994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGC551.6370905E-1136.03347412
ACTCTGC950.036.03347412
CTGGCGA251.4411884E-436.03347412
CGGTTAC200.00209722836.0334712
TAACGTG200.00209722836.0334712
CGACGCA251.4446401E-436.018829
ACGCAGT309.991016E-636.0188210
CATGACG251.4446401E-436.018829
CACGCAT309.991016E-636.018829
TTCGTCC950.036.0188210
GTGGACC950.036.0188211
CGGGGTA251.4446401E-436.0188211
TACGCAG309.991016E-636.018829
AACGCAC251.4446401E-436.0188211
GACGGTA1650.036.0188210
ATAAGCG309.991016E-636.018829
CGCAGGT251.4446401E-436.018829
ACGCTAA251.4446401E-436.0188211
TCCCGCG502.401066E-1036.018829
TAAGGCG309.991016E-636.0188210
Produced by FastQC (version 0.11.5)