FastQCFastQC Report
Thu 26 May 2016
SRR2099092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126504
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACTGAAGTAACAAGAGAGAGGAAAAGAGCTCGTCTCAGACTTAT34270.3042155198738753No Hit
TGCACTGACTTTGGGGCGAGGAAGAATGCCAAGAAAGGCCAGGGCGG18670.16573398762898311No Hit
TGGTCTGACGTCATCTCCGGCCACGCCTGCGCGTTTGATGTCCGAAG16840.14948903865410154No Hit
TGCACTGATGTTTTGAAGACGCGGCGCGTGCGCGGGCGGGGCCCGCG16220.14398528544949685No Hit
TGCACTGATTAGCGATAATGGGCTGGGCACGATGGCTCATGCCTGTA15820.14043447693039704No Hit
TGCACTGAGACTTGGAAGGAGCTTGCACTGATTGTCGGACTGTAGAA15750.1398130854395546No Hit
TGCACTGAACGTCTTCCCAGGAGCTTGCACTGATTTTTTTTTTTTTT15330.1360847364944998No Hit
TGCACTGAGCTAAATTTGCACTGATTTTTTTTTTTTTTTTTTTTTTT14530.1289831194563002No Hit
TGGTCTGACGTCATCTCGCGGCCACGCCTGCGCGTTTGATGTCCGAA14130.1254323109372004No Hit
TGCACTGATTTCGTTGGGTCAAACGAAAAAGCTTGCACTGATTTTTT13700.12161519177916812No Hit
TGCACTGAGTACTGACGGCAACACCAATAATGAACCCTAGTGTAAAC13490.11975101730664073No Hit
TATACTGAACGATGTCTTCAGAGTCATGTGTCGTGGTGCCTCTATTT13090.11620020878754093No Hit
TGCACTGAGGAAAGAACCGAAGGTGCCTGAGGAACGCCTCGGGGAGA12850.11406972367608104No Hit
TGCACTGACAACACGCATGCCGATCAACGGCGCGCGCCGCCTGCGGG12840.11398095346310355No Hit
TGCACTGACGAATCAACGCGAGAGCGACGGCTGCAGCCGTGTAAAGC12540.11131784707377869No Hit
TGCACTGACGAAGAAGGTCTTCCCTGCGGTGGGTGGGGAAGGACAGG11380.10102050236838929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGG304.1043495E-641.005158
CGACACG200.00111365940.9887710
CGTCCGG304.1156363E-640.9887710
CGTCATA650.040.9887710
CGAGCGT256.7514244E-540.98694611
CGCCCGC200.001114388840.98331527
CACTGAC229500.040.3923233
CACTGAG285750.040.3845143
CTGAACG23150.040.300225
CACTGAA299200.039.932863
ACTGAAC74600.039.7719354
ACTGACT52850.039.767284
ACTGATA66100.039.5818634
ACTGACA65100.039.5284464
ACTGACC56950.039.4966164
ACTGAAT67150.039.4209634
ACTGAGG87200.039.2922554
CTGATAC13750.039.219325
ACTGAGA81550.039.022474
TGAACGT10250.039.008366
Produced by FastQC (version 0.11.5)