FastQCFastQC Report
Thu 26 May 2016
SRR2099091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389510
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACTGAAGTAACAAGAGAGAGGAAAAGAGCTCGTCTCAGACTTAT15740.40409745577777206No Hit
TGCACTGACTTTGGGGCGAGGAAGAATGCCAAGAAAGGCCAGGGCGG8640.22181715488690917No Hit
TGGTCTGACGTCATCTCCGGCCACGCCTGCGCGTTTGATGTCCGAAG8570.22002002515981617No Hit
TGCACTGATTAGCGATAATGGGCTGGGCACGATGGCTCATGCCTGTA7460.19152268234448408No Hit
TGCACTGAGACTTGGAAGGAGCTTGCACTGATTGTCGGACTGTAGAA7230.18561782752689276No Hit
TGGTCTGACGTCATCTCGCGGCCACGCCTGCGCGTTTGATGTCCGAA7030.18048317116376986No Hit
TGCACTGAGCTAAATTTGCACTGATTTTTTTTTTTTTTTTTTTTTTT6640.17047059125568023No Hit
TGCACTGAGTACTGACGGCAACACCAATAATGAACCCTAGTGTAAAC6480.1663628661651819No Hit
TGCACTGATGTTTTGAAGACGCGGCGCGTGCGCGGGCGGGGCCCGCG6270.16097147698390285No Hit
TGCACTGACAACACGCATGCCGATCAACGGCGCGCGCCGCCTGCGGG6100.15660701907524838No Hit
TATACTGAACGATGTCTTCAGAGTCATGTGTCGTGGTGCCTCTATTT6000.15403969089368694No Hit
TGCACTGAGGAAAGAACCGAAGGTGCCTGAGGAACGCCTCGGGGAGA5970.1532694924392185No Hit
TGCACTGACGAATCAACGCGAGAGCGACGGCTGCAGCCGTGTAAAGC5780.14839156889425176No Hit
TGCACTGAACGTCTTCCCAGGAGCTTGCACTGATTTTTTTTTTTTTT5570.14300017971297269No Hit
TGCACTGACGAAGAAGGTCTTCCCTGCGGTGGGTGGGGAAGGACAGG5110.13119047007779003No Hit
TGCACTGATGTAAAGAACCGACGATCAGTCACTGGCCCTAGCTTGCA5110.13119047007779003No Hit
TGCACTGATTAAAGACTCTTCCATCATAATGATAGAAGTATACTACT4830.12400195116941798No Hit
TGCACTGACTTGATAGGGAACACGTGGACCAAAGGGATCCAGGCAGC4780.12271828707863726No Hit
TGCACTGACTTTCCGCGTTCGAACCTCGGGGTTGCAATAACGCGCGA4710.12092115735154425No Hit
TGCACTGATTGTAGATTGGGGCGTATTCATACTTCTGCTTGTACCGT4600.11809709635182666No Hit
TGCACTGAGTACTGACGCAACACCAATAATGAACCCTAGTGTAAACT4100.10526045544401941No Hit
TGCACTGACGAAGAAGGCTTCCCTGCGGTGGGTGGGGAAGGACAGGA4090.10500372262586327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAG200.001109517941.013117
GCGCGGT200.001109517941.013119
CGTGATA200.00111021941.00783514
CGTAGTC200.00111021941.00783514
CCTAGCG352.508532E-741.0078314
CGTTGCA256.7230474E-541.0078316
TGATCGC506.002665E-1140.9973076
CTATGAC200.001111622640.99730712
CCCTCCG200.001111622640.99730724
ACACCCG256.733236E-540.99730711
TAGCGAT1000.040.99730710
GACCGCG200.001111622640.9973077
TTACTAG200.001111622640.99730710
CCGCTTG200.001111622640.99730731
CGACACG200.001111622640.99730710
TGCGGAC256.733236E-540.99730730
ATCGTGA352.513607E-740.9973078
CTAGTTG506.002665E-1140.9973079
GAGCGAA900.040.9973077
TTGCGTC200.001111622640.99730730
Produced by FastQC (version 0.11.5)