FastQCFastQC Report
Thu 26 May 2016
SRR2099090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439751
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGGACAATTGAGTGAGTCGTATTACCGGCCTCACTATAGGT128732.9273384256090376No Hit
CTATAGTGGACAATTGAGATCGTCATTCGGACTGTAGAACTCTGAAC61901.4076147638095196Illumina DpnII expression Adapter 1 (95% over 22bp)
CTAGACTCCGCGAGGGAGCCCACTCATCGAACTCCTGCCAGGTCTCT27160.6176222453160993No Hit
CTAGACTGAGGGTGTATAGTTCAAGTACCTAGACTGTTTTTTTTTTT22610.5141546011265467No Hit
CTATAGTGGACAATTGAGGTGAGTCGTATTACCGGCCTCACTATAGG18260.41523498525301816No Hit
CTAGACTGAGTTAGACGGTCCGACGATCGTCGGACTGTAGAACTCTG16670.379078160140625Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGACTGAGGACGCAAAGTCCGACGATCGTCGGACTGTAGAACTCT16410.3731657233297935Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTATAGTGGACAATTGAGGATCGTCATTCGGACTGTAGAACTCTGAA16210.3686176950137691Illumina DpnII expression Adapter 1 (95% over 21bp)
CTAGATTGGCCAGGCATACCTAGACTGTTTTTTTTTTTTTTTTTTTT15070.34269393361243067No Hit
CTCGATTGACTAGCATCGTCTGACGATCGTCAAGCCCGTACGTACCT14870.33814590529640637No Hit
CTATAGTGAAATTCTGATCAGAATTTTGACGATCGTCGGACTGTAGA14730.33496228547518936No Hit
CTAGACTGAAAGAGATGCTTGACGGGATGTCTGGCTCGACGCGATGA13580.30881112265804966No Hit
CTATAGTGGACAATTGATCTGAACCCCTATAGTGAGTCGTATTACCG13090.2976684532837901No Hit
CTATAGTGGACAATTGACTCTGAACCCCTATAGTGAGTCGTATTACC12490.28402436833571726No Hit
CTAGACTGCCACGAGGGCGACATGCTAGCGAATGCCATGACGAGTCC10920.24832234605492656No Hit
CTATAGTGGACAATTGACGATCGTCATTCGGACTGTAGAACTCTGAA10760.2446839234021071Illumina DpnII expression Adapter 1 (95% over 21bp)
CTAGACTGGACTTAAGAGTCCGACGATCGTCAAAGAGACAGGTACCT10710.24354691632310102No Hit
CTAGACTGTCAGTCTCTATACGACTCACTATAGTGAGTCGTATTACC10680.24286471207569738No Hit
CTAGACTGGACCTGTACACCTGGGCCATGGGAGGCTGGCGCTATGTT10000.22740141580121476No Hit
CTAGACGTGCACAACGGCGACGATCGTCACAGCTGCAGGTACCTAGA9810.2230807889009917No Hit
CTAGACTGTGAGGCATGTGTGTACCTAGACTGTAGAACTCTGAACCC9350.2126203237741358No Hit
CTATAGTGGACAATTGATGATCGTCATTCGGACTGTAGAACTCTGAA9030.20534347846849696Illumina DpnII expression Adapter 1 (95% over 21bp)
CTAGACTGGCGCGACGGAGTCCGACGATCGTCACATTAGTTTGTACC8560.19465561192583983No Hit
CTAGACTGGGACCAAAGTGAGGATGTGGGGGGCAAGCAGGCGCGCCG7800.17737310432494754No Hit
CTAGACTGAATAAGGATGTCCGACGATCGTCACAGGGCCAGGTACCG7160.16281941371366979No Hit
CTAGACTGAAAGAGATGGCTTGACGGGATGTCTGGCTCGACGCGATG6610.15031233584460296No Hit
CTAGACTGTCGCCAATACGATCGTCGGACTGTAGAACTCTGAACCCC5830.13257502541210822Illumina DpnII expression Sequencing Primer (96% over 27bp)
CTAGACTGAAAGAGATGTCTTGACGGGATGTCTGGCTCGACGCGATG5650.12848179992768635No Hit
CTAGACTGCTCCGTGGTCTGGGCTGGCCTTCAGGGCCGAAGAGATCT5630.12802699709608392No Hit
CTATAGTGGACAATTGATAGAACTCTGAACCCCTATAGTGAGTCGTA5450.12393377161166205No Hit
CTAGACTGCGGTAATTGCCGACGATCGTCACAGCTATGGGTACCTAG5140.11688432772182439No Hit
CTATAGTGGACAATTGAAGTGAGTCGTATTACCGGCCTCACTATAGG4960.11279110223740253No Hit
CTATAGTGGACAATTGAACAGTCCGATCGTCATTCGGACTGTAGAAC4810.10938008100038431No Hit
CTATAGTGGACAATTGATAGTGAGTCGTATTACCGGCCTCACTATAG4780.10869787675298066No Hit
CTATAGTGGACAATTGAGTCTGACGATCGTCGGACTGTAGAACTCTG4660.1059690597633661Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGGACAATTGAACTCTGAACCCCTATAGTGAGTCGTATTAC4650.10574165834756487No Hit
CTATAGTGGACAATTGACGTGAGTCGTATTACCGGCCTCACTATAGG4620.10505945410016122No Hit
CTATAGTGGACAATTGAGAACTCTGAACCCCTATAGTGAGTCGTATT4580.10414984843695636No Hit
CTATAGTGGACAATTGATGAACCCCTATAGTGAGTCGTATTACCGGC4580.10414984843695636No Hit
CTAGACTGGGGTCGTGTAGTGTACATTTTTAAATGCTAGCCTATTAA4490.10210323569474543No Hit
CTAGACTGGACTTAAGAGGTCCGACGATCGTCAAAGAGACAGGTACC4470.101648432863143No Hit
CTAGACTGAGTTAGACGGGTCCGACGATCGTCGGACTGTAGAACTCT4460.1014210314473418Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTAGACTGAGGCGGAAACGTCAAAGAAAGAAGTACCTAGACTGTTTT4400.1000566229525345No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACC256.7114655E-541.02128618
CTGGTAA1300.041.0072866
GTCGCGT200.001110448241.0072828
GCGAACC553.6379788E-1241.0072828
GTTAGCA200.001110448241.0072828
CTGCGGA900.041.0072826
TGACATA401.5446858E-841.0072827
TGCGACA950.041.0072827
GCGATAG200.001110448241.0072828
TGCTACC352.509787E-741.0072827
CGACTGC553.6379788E-1241.0072823
GTAACGG2100.041.0072828
GCGTAAG256.724994E-541.0072828
GTTCCGG304.097161E-641.007288
GACTCGC304.097161E-641.007284
AACGGCG1250.041.0026213
CGACGTT256.729508E-541.00261716
TCTCTAG200.0011110741.00261713
CGACGGG256.729508E-541.00261713
TTACTCG200.0011110741.00261713
Produced by FastQC (version 0.11.5)