FastQCFastQC Report
Thu 26 May 2016
SRR2099089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175776
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGGACAATTGAGTGAGTCGTATTACCGGCCTCACTATAGGT59633.3923857636992536No Hit
CTATAGTGGACAATTGAGATCGTCATTCGGACTGTAGAACTCTGAAC27341.5553886764973603Illumina DpnII expression Adapter 1 (95% over 22bp)
CTAGACTCCGCGAGGGAGCCCACTCATCGAACTCCTGCCAGGTCTCT13000.7395776442745312No Hit
CTAGACTGAGGGTGTATAGTTCAAGTACCTAGACTGTTTTTTTTTTT11190.636605679956308No Hit
CTAGACTGTCAGTCTCTATACGACTCACTATAGTGAGTCGTATTACC10380.5905243036592026No Hit
CTATAGTGGACAATTGAGGTGAGTCGTATTACCGGCCTCACTATAGG8270.4704851629346441No Hit
CTCGATTGACTAGCATCGTCTGACGATCGTCAAGCCCGTACGTACCT7260.41302566903331517No Hit
CTAGATTGGCCAGGCATACCTAGACTGTTTTTTTTTTTTTTTTTTTT7160.4073366102312033No Hit
CTATAGTGGACAATTGAGGATCGTCATTCGGACTGTAGAACTCTGAA7090.40335426906972516Illumina DpnII expression Adapter 1 (95% over 21bp)
CTAGACTGAGTTAGACGGTCCGACGATCGTCGGACTGTAGAACTCTG6710.3817358456217003Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGACTGAGGACGCAAAGTCCGACGATCGTCGGACTGTAGAACTCT6560.3732022574185327Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTAGACTGAAAGAGATGCTTGACGGGATGTCTGGCTCGACGCGATGA6330.3601174221736756No Hit
CTATAGTGGACAATTGATCTGAACCCCTATAGTGAGTCGTATTACCG5970.3396368104860732No Hit
CTATAGTGGACAATTGACTCTGAACCCCTATAGTGAGTCGTATTACC5710.3248452576005826No Hit
CTATAGTGAAATTCTGATCAGAATTTTGACGATCGTCGGACTGTAGA5510.313467139996359No Hit
CTAGACTGGACTTAAGAGTCCGACGATCGTCAAAGAGACAGGTACCT5160.2935554341889678No Hit
CTAGACTGCCACGAGGGCGACATGCTAGCGAATGCCATGACGAGTCC5070.2884352812670672No Hit
CTATAGTGGACAATTGACGATCGTCATTCGGACTGTAGAACTCTGAA4700.2673857636992536Illumina DpnII expression Adapter 1 (95% over 21bp)
CTAGACGTGCACAACGGCGACGATCGTCACAGCTGCAGGTACCTAGA4630.2634034225377753No Hit
CTAGACTGGACCTGTACACCTGGGCCATGGGAGGCTGGCGCTATGTT4570.2599899872565083No Hit
CTAGACTGTGAGGCATGTGTGTACCTAGACTGTAGAACTCTGAACCC4500.25600764609503No Hit
CTAGACTGGCGCGACGGAGTCCGACGATCGTCACATTAGTTTGTACC4140.23552703440742764No Hit
CTATAGTGGACAATTGATGATCGTCATTCGGACTGTAGAACTCTGAA4100.2332514108865829Illumina DpnII expression Adapter 1 (95% over 21bp)
CTAGACTGAATAAGGATGTCCGACGATCGTCACAGGGCCAGGTACCG3660.2082195521572911No Hit
CTAGACTGGGACCAAAGTGAGGATGTGGGGGGCAAGCAGGCGCGCCG3560.2025304933551793No Hit
CTAGACTGAAAGAGATGGCTTGACGGGATGTCTGGCTCGACGCGATG3270.18603222282905515No Hit
CTAGACTGAAAGAGATGTCTTGACGGGATGTCTGGCTCGACGCGATG2770.15758692881849626No Hit
CTAGACTGCTCCGTGGTCTGGGCTGGCCTTCAGGGCCGAAGAGATCT2680.15246677589659566No Hit
CTATAGTGGACAATTGATAGAACTCTGAACCCCTATAGTGAGTCGTA2470.14051975241216094No Hit
CTATAGTGGACAATTGAAGTGAGTCGTATTACCGGCCTCACTATAGG2430.1382441288913162No Hit
CTAGACTGACGGCGGTGGTCCGACGATCGTCATCAATCGGACTGTAG2400.1365374112506827No Hit
CTAGACTGCGGTAATTGCCGACGATCGTCACAGCTATGGGTACCTAG2390.1359685053704715No Hit
CTATAGTGGACAATTGATAGTGAGTCGTATTACCGGCCTCACTATAG2300.1308483524485709No Hit
CTATAGTGGACAATTGAACAGTCCGATCGTCATTCGGACTGTAGAAC2210.1257281995266703No Hit
CCAGACTTACATACTAAATCGTCAGACCTCGAGGTACCTAGACTGTT2180.12402148188603676No Hit
CTATAGTGGACAATTGACGTGAGTCGTATTACCGGCCTCACTATAGG2160.12288367012561442No Hit
CTATAGTGGACAATTGACTGAACCCCTATAGTGAGTCGTATTACCGG2160.12288367012561442No Hit
CTAAGCTGATCATGAACCACTGCCGTGCACCGTGCGCAACTGGCGAT2120.1206080466047697No Hit
CTATAGTGGACAATTGAGAACTCTGAACCCCTATAGTGAGTCGTATT2100.11947023484434735No Hit
CTAGACTGTGTACTAATAAAGCTCACTTGCCCACTTCCTTCCACAAG2080.118332423083925No Hit
CTAGACTGGCTCTGTCGGATCGTCACAGGACTATGTACCTAGACTGT2070.11776351720371382No Hit
CTATAGTGGACAATTGAACTCTGAACCCCTATAGTGAGTCGTATTAC2060.11719461132350265No Hit
CTATAGTGGACAATTGATGAACCCCTATAGTGAGTCGTATTACCGGC2020.11491898780265793No Hit
CTAGACTGGGGTCGTGTAGTGTACATTTTTAAATGCTAGCCTATTAA2010.11435008192244675No Hit
CTAGACTGACCTGCAGCGTCCGACGATCGTCATCGGGCTGTAGAACT1970.11207445840160203No Hit
CTATAGTGGACAATTGATGTGAGTCGTATTACCGGCCTCACTATAGG1960.11150555252139085No Hit
CTAGACTGGACTTAAGAGGTCCGACGATCGTCAAAGAGACAGGTACC1930.10979883488075734No Hit
CTTGACCGAAACTAAAAGCTGGTACCGTGTTGGCTTATTTTTCTTTG1890.10752321135991262No Hit
CTATAGTGGACAATTGAAACTCTGAACCCCTATAGTGAGTCGTATTA1810.1029719643182232No Hit
CTAGACTGACGTGATCTGTACAAGCTAAGTTTTCCTTAAACTAAGCT1810.1029719643182232No Hit
CTAGACTGCCGCATTGCAGGACTCAGAAACAAACCGAAGAGATACTT1800.10240305843801202No Hit
CTATAGTGGACAATTGAATGATCGTCATTCGGACTGTAGAACTCTGA1790.10183415255780083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGA1100.041.00000411
TTGATCT750.041.00000414
CCAGGCA1100.041.00000410
CACGAGG1100.041.00000410
CTGCGCA553.6379788E-1241.0000046
AACGGCG553.6379788E-1241.00000413
CTCGGCT553.6379788E-1241.0000041
CCACGAG1100.041.0000049
GCCACGA1100.041.0000048
CGGACAC200.001109598241.012
CTTCGTA200.001109598241.010
CTAGAAT200.001109598241.01
CAGCGGA200.001109598241.011
CGGAACC200.001109598241.012
TGATAGC459.495125E-1041.07
AGCCGGA256.715524E-541.024
TCTGAGG256.715524E-541.013
GTACCGG200.001109598241.011
GAAGCGG200.001109598241.08
ACTGCGG1400.041.05
Produced by FastQC (version 0.11.5)