FastQCFastQC Report
Thu 26 May 2016
SRR2099087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356774
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGCAGAAAAACTCTCGTTTGCCAGTCCTGATGCATTTTTTTTT19310.541238991630556No Hit
CTGATGCACGGATGTTGGAGCGAGGCAAAGGATTTCTTGAAGGAAAG15520.43500927758188657No Hit
CTGATGCAGCATAGGGTCGACGATCAGCTCATGTGTGCAGTCCTGAT12220.3425137481991401No Hit
CTGATGCAGAGGAGTAAGGCAAGGGAAAGCTCCCTAAGCAACCTGTC6780.19003626945909735No Hit
CTGATGCAAGCCACAGGGTTTGAGACCATCTTGGCCATGGTGAAACT6210.1740597689293502No Hit
CTGATGCATTCAACTGAGACTGTTGCCTTTCTATATTGGTAATGACT5530.1550000840868449No Hit
CTGATGCACTCTTGAATACGAATGGGTGAGTCAGATGATTCCATTCT5490.15387892615493282No Hit
CTGATGCACTAAGTAGCGACCAAGGACCAGGTAGCTAACTCAGCCTT5130.14378850476772412No Hit
CTGATGCACCGGCACACGTACACTTTCTTTGCACAATGTCTTTGGTT5040.14126589942092194No Hit
CTGATGCAGTAAAACGAGTTCTGGGCAAGGGAAAGCTCCCTAAGCAA4960.13902358355709776No Hit
CTGATGCAGCAGCTCTAGAAGTAATGTAGGGGTGGGGGGCGTTTGGT4440.12444853044224075No Hit
CTGATGCAGAGAAGCAACATAATCCTGCACACAAGCTGGGCAGTGAT4060.113797530089076No Hit
CTGATGCAGACAGGCTGGGTATTGTTGGGGACTTGGGGAAGGGAAAC3670.10286624025293321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC304.0985396E-641.00210617
TGAGTTA304.0985396E-641.00210613
AGCGACC600.041.00210615
GATGCGA2300.041.0021063
GATCGCG200.00111085741.002138
CGACGTG200.00111085741.002113
CGCATTA200.00111085741.00215
GGGTACG401.5450496E-841.002114
TCGATCT200.00111085741.002114
ATTCCTT200.00111085741.002117
TTATACG200.00111085741.002112
CCACTCG256.72745E-541.002117
GAGGTAG200.00111085741.002115
CGTACTC256.72745E-541.002112
TCATCCG256.72745E-541.002115
CGTGTAT200.00111085741.002124
CATCGGG256.72745E-541.002113
GCGGATG200.00111085741.002115
GCGTGTA200.00111085741.002123
TATACGT200.00111085741.002113
Produced by FastQC (version 0.11.5)