SRR2099086_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501188
Sequences flagged as poor quality	0
Sequence length	43
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACGTGCATTTCTTGACAGGGCTGCAGATGATCTGCAGGGCCAG	4859	0.9694964763721398	No Hit
ACGAGGATTCAGCACGCTCGGCGCCTGCCAGCACGCCGCCGAC	2317	0.4623015714661964	No Hit
ACGTCCAGATCCGGGCTCCCGGCGGTGTGCTGCGGGGCGATCC	2279	0.45471958626303904	No Hit
ACGGGCATTATTGCAGTCCGACGATCGGCTTACGTGCATTTTT	2161	0.43117552694797157	No Hit
ACGTGCATCGGTGGGGTTCAGTGAGGTCGGGCAGGTTCGCTGT	2113	0.42159828248082554	No Hit
ACGTGCATGGTTACGTCCGACGATCCTTCCACGTGCATTTTTT	1715	0.3421869637740728	No Hit
AGGAGCATGACCGACTGCTGCTCCAGCGCTTCGGCGAACCCCT	1679	0.3350040304237133	No Hit
ACGAGCTTCAACTAAATAACAATACAGGACTCTTTCGAGGCCC	1429	0.2851225488239942	No Hit
ACGTGCCTGAGATAACAGAGCCTGTGGACGTTCTGGCTACTCT	1345	0.2683623710064886	No Hit
ACGTGCATTATCTGGCTTCGCGAACCTCATCCGACGCTTTCAA	1118	0.22306998571394368	No Hit
ACGTGCATATCGTGAAGGCGGCAGCTCTGGCAAAGCAGCTGGA	1110	0.22147377830275267	No Hit
ACGTGCATTTCTTGGACAGGGCTGCAGATGATCTGCAGGGCCA	915	0.18256622265497177	No Hit
ACGGGCATTATTGGCAGTCCGACGATCGGCTTACGTGCATTTT	913	0.18216717080217404	No Hit
ACGTGCATCGGATGCTGGATGGCAAGAGCTCTGGCTGAAGAAG	846	0.1687989337334493	No Hit
AGGAGCATGACCGGACTGCTGCTCCAGCGCTTCGGCGAACCCC	807	0.16101742260389315	No Hit
ACGTGCATAGTATGCCTTAGGTCACTCTAAGGGGGACCTGGCT	807	0.16101742260389315	No Hit
ACGTGCATATAACGTCTGCAACCCTATCATTACCAAGCTGTAC	773	0.15423354110633136	No Hit
ACGTGCATGCGGAGCATGATCTATGATTCTTTAGATTATGCAA	768	0.153235911474337	No Hit
ACATTCATCGTCCAGGTTAAACTCTTTTTCGAGCCTATATTTT	722	0.14405771885998866	No Hit
ACGTGCATTCTACCGACGATCTCTTGACGTGCATTTTTTTTTT	719	0.14345914108079205	No Hit
ACGTGCATGCGGTACAGTCCGACGATCCTAAAACGTGCACGTT	716	0.1428605633015954	No Hit
ACGAGCTTCAACTTCCAAGGAAGGCAGCAGGCGCGCAAATTAC	710	0.14166340774320216	No Hit
ACGTGCATGGAGGAGAAGAATGAGCCTAAACACAGACTCGCAA	635	0.12669896326328645	No Hit
ACGTGCATACAAGCAGTCCGACGATCGGCGCACGTGCACTGAT	621	0.12390560029370216	No Hit
ACGTGCATCTGTGGCTGGGGGGCTGGAACCCCCTCACCCGCTT	601	0.11991508176572463	No Hit
ACGTGCATTCTCCCAATCTTCTCCCTCCACCTCTCGATGGGGT	600	0.11971555583932578	No Hit
ACGTGCTAAGTCGCGACGATCAGTCGCTCCTGGGAGCTTGCAC	596	0.11891745213373026	No Hit
ACGTGCATGTCTCTCGAGATCACGCCACTGCACTCCAGCCTGG	578	0.11532598545855048	No Hit
ACGTGCAGAGTAAAGACAAACAGAGAGCCAAATCATGAGTGAA	545	0.10874162988738756	No Hit
ACGAGCTTCAACTAATAACAATACAGGACTCTTTCGAGGCCCT	529	0.10554921506500554	No Hit
ACGTGCATCGACGGAGACTTTGAAAACACAGATGACTTTGGTG	526	0.10495063728580892	No Hit
GCGTCCATCATTAGCTCAGAAGTACTGCAGCTTTTCTCAGTCT	525	0.10475111135941004	No Hit
ACGTGCATTTCTTTGACAGGGCTGCAGATGATCTGCAGGGCCA	509	0.10155869653702802	No Hit
ACGTGCATTTGACGCAACCTGTCATCGTGAAGGCCAAATTCTT	507	0.10115964468423028	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTC	25	1.2226202E-4	37.051296	8
ATACTAG	125	0.0	37.051296	7
ATGAGCG	45	2.579327E-9	37.051296	7
ATCCCGT	20	0.0018284611	37.051296	7
TTGAACG	20	0.0018284611	37.051296	8
ATTGGTA	25	1.2226202E-4	37.051296	7
CATAACC	235	0.0	37.051296	6
CAGTCGA	25	1.2226202E-4	37.051296	6
ATTATCC	40	3.7794962E-8	37.051296	7
CGTTTTC	25	1.2226202E-4	37.051296	7
TATGTCG	45	2.579327E-9	37.051296	8
CATCACG	65	0.0	37.051296	6
TGTAACG	40	3.7794962E-8	37.051296	8
TACCGTA	20	0.0018284611	37.051296	8
TACTTCG	30	8.224075E-6	37.051292	8
TAAAGGG	30	8.224075E-6	37.051292	8
TTTCGTG	35	5.562142E-7	37.051292	8
TATGCCG	55	1.2732926E-11	37.051292	8
ATATCCG	30	8.224075E-6	37.051292	7
TACTAGA	35	5.562142E-7	37.051292	8

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content