FastQCFastQC Report
Thu 26 May 2016
SRR2099085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234662
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCATTTCTTGACAGGGCTGCAGATGATCTGCAGGGCCAG22850.9737409550758112No Hit
ACGAGGATTCAGCACGCTCGGCGCCTGCCAGCACGCCGCCGAC10900.4644978735372578No Hit
ACGTCCAGATCCGGGCTCCCGGCGGTGTGCTGCGGGGCGATCC10840.4619410045086124No Hit
ACGGGCATTATTGCAGTCCGACGATCGGCTTACGTGCATTTTT9610.40952518942138055No Hit
ACGTGCATCGGTGGGGTTCAGTGAGGTCGGGCAGGTTCGCTGT8660.36904142980116084No Hit
ACGTGCATGGTTACGTCCGACGATCCTTCCACGTGCATTTTTT7580.32301778728554265No Hit
AGGAGCATGACCGACTGCTGCTCCAGCGCTTCGGCGAACCCCT7520.32046091825689715No Hit
ACGAGCTTCAACTAAATAACAATACAGGACTCTTTCGAGGCCC6560.27955101379856984No Hit
ACGTGCCTGAGATAACAGAGCCTGTGGACGTTCTGGCTACTCT6310.26889739284588043No Hit
ACGTGCATTATCTGGCTTCGCGAACCTCATCCGACGCTTTCAA5610.23906725417835012No Hit
ACGTGCATATCGTGAAGGCGGCAGCTCTGGCAAAGCAGCTGGA5030.21435085356811073No Hit
ACGGGCATTATTGGCAGTCCGACGATCGGCTTACGTGCATTTT4560.19432204617705465No Hit
ACGTGCATTTCTTGGACAGGGCTGCAGATGATCTGCAGGGCCA4240.18068541135761224No Hit
AGGAGCATGACCGGACTGCTGCTCCAGCGCTTCGGCGAACCCC3830.16321347299520161No Hit
ACGTGCATATAACGTCTGCAACCCTATCATTACCAAGCTGTAC3590.15298599688061978No Hit
ACGTGCATGCGGAGCATGATCTATGATTCTTTAGATTATGCAA3580.1525598520425122No Hit
ACGTGCATAGTATGCCTTAGGTCACTCTAAGGGGGACCTGGCT3530.15042912785197432No Hit
ACGTGCATCGGATGCTGGATGGCAAGAGCTCTGGCTGAAGAAG3510.14957683817575917No Hit
ACGTGCATGCGGTACAGTCCGACGATCCTAAAACGTGCACGTT3280.13977550689928492No Hit
ACGTGCATTCTACCGACGATCTCTTGACGTGCATTTTTTTTTT3280.13977550689928492No Hit
ACGAGCTTCAACTTCCAAGGAAGGCAGCAGGCGCGCAAATTAC3210.13679249303253188No Hit
ACGTGCATCGTGTGTCGAGCACCACCAGTTGGCTGAAGTTGTG3160.13466176884199402No Hit
ACATTCATCGTCCAGGTTAAACTCTTTTTCGAGCCTATATTTT3030.12912188594659552No Hit
ACGTGCATCTGTGGCTGGGGGGCTGGAACCCCCTCACCCGCTT2900.12358200305119704No Hit
ACGTGCATGGAGGAGAAGAATGAGCCTAAACACAGACTCGCAA2890.12315585821308947No Hit
ACGTGCATGTCTCTCGAGATCACGCCACTGCACTCCAGCCTGG2790.11889440983201371No Hit
ACGTGCATACAAGCAGTCCGACGATCGGCGCACGTGCACTGAT2750.1171898304795834No Hit
ACGTGCTAAGTCGCGACGATCAGTCGCTCCTGGGAGCTTGCAC2710.1154852511271531No Hit
ACGTGCATTCTCCCAATCTTCTCCCTCCACCTCTCGATGGGGT2610.11122380274607735No Hit
ACGAGCTTCAACTAATAACAATACAGGACTCTTTCGAGGCCCT2540.10824078887932431No Hit
ACGTGCAGAGTAAAGACAAACAGAGAGCCAAATCATGAGTGAA2530.10781464404121674No Hit
GCGTCCATCATTAGCTCAGAAGTACTGCAGCTTTTCTCAGTCT2460.1048316301744637No Hit
ACGTGCATTTCTTTGACAGGGCTGCAGATGATCTGCAGGGCCA2400.10227476114581824No Hit
ACGTGCATCGACGGAGACTTTGAAAACACAGATGACTTTGGTG2350.10014403695528036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTC251.225758E-437.02495213
CTACGAC200.00183287237.02495213
CGTAAGA251.225758E-437.02495213
CGATGAG251.225758E-437.02495213
TCGGTTA251.225758E-437.02495213
TGTTTCG200.00183479337.017068
TGTCAAT200.00183479337.017068
TCGTTCA200.00183479337.017068
CGAGTGT403.7956852E-837.017069
GTCTGCG200.00183479337.017069
TTAGCTC355.5856435E-737.0170611
CCGATAC200.00183479337.017069
TAGCGCG200.00183479337.017068
GACCGGA700.037.017069
GTCTCTC355.5856435E-737.017069
GTCTCGT200.00183479337.017069
TATGCCG200.00183479337.017068
AGTATGC403.7956852E-837.017069
TAGTTCG200.00183479337.017068
TCGTGTG355.5856435E-737.017068
Produced by FastQC (version 0.11.5)