FastQCFastQC Report
Thu 26 May 2016
SRR2099084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305704
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTAGCGGCCACGTCCGACGATCTGCCGGATCTAGCTTTTT89022.9119671316044275No Hit
GCTCGAGCTTGGAGATCTAGCTTTTTTTTTTTTTTTTTTTTTT23880.7811477769345511No Hit
GATCTAGCGTCGACTACAGTCCGACGATCATACAGATCTAGCT22780.7451652578965273No Hit
GATCTAGCAGGAAGGCCGATCTAGCTTTTTTTTTTTTTTTTTT19130.625768717452176No Hit
GATCTAGCTTACTACAGTCCGACGATCAACTTGATCTAGCTTT18760.6136655065030225No Hit
GATCTAGCAGGAACCGATCTAGCTTTTTTTTTTTTTTTTTTTT16990.555766362232748No Hit
GATCTGGACAAATCGCTCGAACTGCGGATCGGCACGACCGTCG16820.5502054274723261No Hit
GATCTAGCAGCCGCAGCCAGCTATAACCAGCGCTCTTCTGTCT14870.4864182346321932No Hit
GATCTAGCTCCGGACGATCCGACCGATCTAGCTTTTTTTTTTT13480.4409494151205087No Hit
GATCTAGCCAAGGCCAAGCCTCTGTGTTCAGAGCCCTTGCCCC12640.4134718551278361No Hit
GATCTAGCCGCCTAGATGCTGGCAACTTTGACCAGAATAAGCT11580.3787977913275587No Hit
GATCTAGCTGATAAACCCAGCAGGGTCACCATTTGCAGCGCAA11540.37748933608981233No Hit
GATCTCCCCCATTAGCGCGTGCGGAGAATGAGCGCAGCCGCCG10830.3542642556198153No Hit
GATCTAGCTCACGACGATCTTCTGGATCTAGCTTTTTTTTTTT10630.3477219794310836No Hit
GATCTAGCTGCACTTACGGGTGGGGGGAGGGAGTGTTCTGCTG10410.3405254756234789No Hit
GATCTAGCCTGAACCTCGCGGGCCGCCGGTGAAATACCACTAC9740.31860885039122816No Hit
GATCTAGCATTGGCATGTATGTTTGTGCAACATTTGTGTTCCT9170.2999633632533431No Hit
GATCTAGCCTAGAGTGGCGCACACCTGTTATCCCAGCTACTCG7620.24926072279067335No Hit
GATCTAGCCTTAAGATCTAGCTTTTTTTTTTTTTTTTTTTTTT7150.2338863737471541No Hit
GATCTAGCTCCTTGTTCTAGCTTTTTTTTTTTTTTTTTTTTTT6830.2234187318451836No Hit
GATCTAGCAGGAATCCGATCTAGCTTTTTTTTTTTTTTTTTTT6430.21033417946772043No Hit
GATCTAGCGTGTGGCGCGCTGCAACGCGACGCCGACCGGATTA6340.2073901551827912No Hit
GATCTAGCGTCTACAGATACAGATCTTTGGTTCGCAGAGCTGT5960.19495983042420117No Hit
GATCTAGCAGGAAGCCGATCTAGCTTTTTTTTTTTTTTTTTTT5810.1900531232826525No Hit
GATCTAGCAGGCGCAGCTGTGTGTGAAAACCTAGCATCAGAAG5100.16682804281265537No Hit
GATCTAGCAGACAGAGAAAATAGGAAACATGGATCTGAGGTAG4910.16061288043336036No Hit
GATCTAGCATAACCAGTCCGACGATCCAGGGGATCGTCGGACT4910.16061288043336036No Hit
GATCTAGCAGCTCTCTACAGTCCGACGATCTCCACGATCGTCG4770.15603328710124825No Hit
GATCTAGCTCCTTGGTGCGGTAGGCCTGGACATGGCAAAGAGA4610.150799466150263No Hit
GATCTAGCAGGAACCCGATCTAGCTTTTTTTTTTTTTTTTTTT4530.14818255567477037No Hit
GATCTAGCGACGAGTCCGACGATCGTAGTGATCGTCGGACTGT4510.14752832805589722No Hit
GATCTAGCGGATTCAGTCCGACGATCCTCTAGATCCTTTCGAT4490.14687410043702404No Hit
GATCTAGCTCCTTCAGTCCGACGATCCCTCTGATCTAGCTTTT4490.14687410043702404No Hit
GATCTAGCTCCTTGTGCGGTAGGCCTGGACATGGCAAAGAGAA4440.14523853138984114No Hit
GATCTGGGGTGCACGCACGAGGAAGGTGGCAGCGAAGCGCTGG4300.14065893805772903No Hit
GATCTAGCTGCACGTTACGGGTGGGGGGAGGGAGTGTTCTGCT4110.13444377567843405No Hit
GATCTAGCTGCACTTTACGGGTGGGGGGAGGGAGTGTTCTGCT4070.1331353204406877No Hit
GATCTAGCCGTGTCAAACGCCGACGTATTGCTCTGAAGAAGCA4030.1318268652029414No Hit
GATCTAGCGCTTGCAGTATTCCCACATGCCAGCAAACTCTCTT4000.13084552377463166No Hit
GATCTAGCTCTTCCAGTCCGACGATCCTTGTGATCGTCGGACT3910.12790149948970245No Hit
GATCTAGCCCAGCAAAGCTGATGCTTTCAGATGATGTAATCCA3910.12790149948970245No Hit
GATCTAGCAACCTCAGTCCGACGATCCTGAAGATCGTCGGACT3880.1269201580613927No Hit
GATCTAGCATCGCCTACAGTCCGACGATCTGGTAGATCTCTGA3700.1210321094915343No Hit
GATCTAGCAGCCGCCAGCCAGCTATAACCAGCGCTCTTCTGTC3650.1193965404443514No Hit
GATCTAGCTTCAACGATCAGCTGGATCTAGCAGATCGTCGGAC3640.11906942663491482No Hit
GATCTAGTATCGGCGTCGGGTCTCAGACCTGGGCGACACCACC3580.11710674377829533No Hit
GATCTAGCTCAGGCAGTCCGACGATCATGCGGATCGTCGGACT3570.11677962996885875No Hit
GATCTAGCACAACGATCAGTCGGACTGTAGAACTCTGAACCCC3560.1164525161594222Illumina NlaIII expression Adapter 1 (95% over 24bp)
GATCTAGCTCATGCAGTCCGACGATCACAGGGATCGTCGGACT3520.11514406092167587No Hit
GATCTAGCGCGCGCAGTCCGACGATCCCAGTGATCGTCGGACT3380.11056446758956377No Hit
GATCTAGCATTGGTGTTGCACATGTATGTTTGTGCAACATTTG3360.1099102399706906No Hit
GATCTAGCATTGGATGTTGCACATGTATGTTTGTGCAACATTT3290.10762044330463454No Hit
GATCTAGCGTATTGGCCCCTGAGCAAGACTGTGCGATTCAATG3220.1053306466385785No Hit
GAGATAGCGTACTGAACTTGGAAGGAGCCAATCAACACGTTTC3200.10467641901970534No Hit
GATCTAGCCACTCCAGTCCGACGATCAGCCTGATCTAAAGGAT3170.1036950775913956No Hit
GATCTGGCTTTGTAGTGGTGGGTCACGTCTGTAATCCCAGCAC3160.10336796378195902No Hit
GATCTAGCCTCACAGACCTGGAACGTCACCTCGCCGCGATCGC3120.10205950854421271No Hit
GGTATAGCCATGCGAAACAGAATCAGTGACGATCTTTTTCCAT3100.10140528092533956No Hit
GATCTAGCTCGGGGTCCGACGATCAGTTGGATCGTCGGACTGT3070.10042393949702981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTC200.00180624937.13898537
AGATAGT308.081392E-637.13898537
TGCATTC200.00180624937.13898537
GGAGTCG200.00180624937.13898537
TGATCCA200.001835588537.01735713
CGTGGTC251.2281093E-437.01735713
TTTCGCT200.001835588537.01735713
ACCCTCG251.2281093E-437.01735713
ATTCCGA251.2281093E-437.01735713
TACTGCG200.001835588537.01735713
CTTTACG452.593879E-937.01735713
GCATAAC501.7826096E-1037.01137
CTCACGG251.2292937E-437.01139
GCGACTT355.59985E-737.01137
ACGCCGG251.2292937E-437.01139
GCGGTGT251.2292937E-437.01137
GCGCGTA251.2292937E-437.01137
GCGCGGG452.5993359E-937.01137
GCGTTTG251.2292937E-437.01137
CTGACGT452.5993359E-937.01139
Produced by FastQC (version 0.11.5)