##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099084_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 305704 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.050009159186665 37.0 35.0 37.0 28.0 37.0 2 32.71792321984665 35.0 32.0 35.0 28.0 37.0 3 33.38088804856986 35.0 33.0 35.0 28.0 37.0 4 36.45871169497291 39.0 35.0 39.0 32.0 39.0 5 35.82136969094287 39.0 35.0 39.0 29.0 39.0 6 35.29183458508884 37.0 34.0 39.0 30.0 39.0 7 35.193177060162775 37.0 35.0 39.0 27.0 39.0 8 36.05935152958417 39.0 35.0 39.0 30.0 39.0 9 30.27434380969827 31.0 30.0 33.0 26.0 34.0 10 30.3684969774684 31.0 30.0 34.0 26.0 34.0 11 30.611316829351267 31.0 30.0 34.0 26.0 34.0 12 34.17966071755685 37.0 35.0 37.0 29.0 37.0 13 34.31869062884359 35.0 35.0 37.0 30.0 37.0 14 31.61401551827912 31.0 31.0 34.0 30.0 34.0 15 31.62030918797268 31.0 31.0 34.0 30.0 34.0 16 31.722519168869233 31.0 31.0 34.0 30.0 34.0 17 35.221792976212285 35.0 35.0 37.0 33.0 37.0 18 35.35984154607071 35.0 35.0 37.0 33.0 37.0 19 34.88322037003114 35.0 35.0 37.0 32.0 37.0 20 34.52125585533719 35.0 35.0 37.0 30.0 37.0 21 34.518226781461806 35.0 35.0 37.0 30.0 37.0 22 36.017523486771516 38.0 35.0 39.0 30.0 39.0 23 36.24777235495774 38.0 35.0 39.0 32.0 39.0 24 36.522577395127314 38.0 35.0 39.0 32.0 39.0 25 36.73243398843326 38.0 35.0 39.0 32.0 39.0 26 36.79024154083689 39.0 36.0 39.0 32.0 39.0 27 38.14349501478554 40.0 37.0 41.0 33.0 41.0 28 38.254193599036974 40.0 37.0 41.0 33.0 41.0 29 38.42990605291393 40.0 38.0 41.0 34.0 41.0 30 38.49402690183969 40.0 38.0 41.0 34.0 41.0 31 38.54535760081647 40.0 38.0 41.0 35.0 41.0 32 38.644610472875726 40.0 38.0 41.0 35.0 41.0 33 38.70652330358779 40.0 38.0 41.0 35.0 41.0 34 38.75358189621333 40.0 38.0 41.0 35.0 41.0 35 38.74892052442886 40.0 38.0 41.0 35.0 41.0 36 38.73593083505613 40.0 38.0 41.0 35.0 41.0 37 38.58219061575903 40.0 38.0 41.0 35.0 41.0 38 38.48899262031246 40.0 38.0 41.0 35.0 41.0 39 38.346917279459866 40.0 38.0 41.0 35.0 41.0 40 38.268347813571296 40.0 38.0 41.0 35.0 41.0 41 38.11150001308455 40.0 37.0 41.0 34.0 41.0 42 37.954567163007354 40.0 37.0 41.0 34.0 41.0 43 37.91252649621856 40.0 37.0 41.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3616963677870828 1101 2 0.8280003228327502 1101 3 0.593093962231336 1101 4 0.5188479029960078 1101 5 0.4906404080527835 1101 6 0.5190762444661985 1101 7 0.5374638150958049 1101 8 0.7057169010095734 1101 9 0.5027927373000836 1101 10 0.6253117818995477 1101 11 0.6301456994603178 1101 12 0.46384812060723846 1101 13 0.5775822081829318 1101 14 0.14320113548423663 1101 15 0.15047390668120997 1101 16 -0.1590789149952272 1101 17 0.11475454248509465 1101 18 -0.13856064647548294 1101 19 -0.09210218894124722 1101 20 -0.3589842208509566 1101 21 -0.3257757643752228 1101 22 -0.4624787626681126 1101 23 -0.0387797408880175 1101 24 -0.39319971082257865 1101 25 -0.08687766767582872 1101 26 -0.12635115426567012 1101 27 -0.09292419284170705 1101 28 -0.03748761095285147 1101 29 0.04523383964973249 1101 30 0.11252191073803886 1101 31 0.09591471129392204 1101 32 0.23733339271582565 1101 33 0.07227823821682478 1101 34 0.0579487828864913 1101 35 0.07599087738982746 1101 36 0.11208930893518954 1101 37 0.12391610897626038 1101 38 0.2504321533831373 1101 39 0.37582797038505333 1101 40 0.25627406732544955 1101 41 -0.023270775192337112 1101 42 0.39148481189855033 1101 43 0.3313318367936162 1102 1 0.3321006418885233 1102 2 0.6487608417829662 1102 3 0.21575473262389266 1102 4 0.4478708758728729 1102 5 0.2583972160320016 1102 6 0.3042457484010015 1102 7 0.19195860844032353 1102 8 0.2904856033411676 1102 9 0.39267270600576154 1102 10 0.2247612026617425 1102 11 0.3564961038180918 1102 12 0.4936865030087816 1102 13 0.5372593913593064 1102 14 0.08101413099509003 1102 15 0.17262258817781628 1102 16 0.11475476489064107 1102 17 0.02037371402231969 1102 18 -0.1855063123374734 1102 19 -0.012757696197930102 1102 20 0.0263584896221829 1102 21 -0.2931426484565378 1102 22 -0.24725917945157505 1102 23 -0.2437910160479433 1102 24 -0.039335406012540375 1102 25 -0.09569697643949837 1102 26 0.0030224154206734966 1102 27 0.13160837961008554 1102 28 0.08181989759241759 1102 29 0.1306196425708137 1102 30 0.1370218500739142 1102 31 0.3160543642951197 1102 32 0.2928724457697385 1102 33 0.40387671701157757 1102 34 0.2076803964267242 1102 35 0.14572951960118274 1102 36 0.14249667899890994 1102 37 0.3924782855214133 1102 38 0.3392988220022133 1102 39 0.23490311441005218 1102 40 0.06910641260054717 1102 41 0.0977980848659854 1102 42 0.38436409865578014 1102 43 0.16865575393897103 1103 1 0.4855320026019143 1103 2 0.7459351099981149 1103 3 0.5061910268991952 1103 4 0.5060032047877243 1103 5 0.7184619237607066 1103 6 0.9560433194467066 1103 7 0.6144984672744584 1103 8 0.6386679117330516 1103 9 0.5446553862256422 1103 10 0.5934222194744834 1103 11 0.48491760545590523 1103 12 0.43682981775071994 1103 13 0.5590781292366671 1103 14 0.2490650923555613 1103 15 0.3609148353227063 1103 16 0.22889469132986662 1103 17 0.30392877595340906 1103 18 0.17026547219544597 1103 19 0.15360716203258562 1103 20 0.011340501839391948 1103 21 0.06271766655098787 1103 22 0.17994060163670866 1103 23 -0.028911457734523083 1103 24 -0.24320096846273742 1103 25 -0.10852584703208379 1103 26 -0.14004685565159036 1103 27 -0.14304534551084203 1103 28 0.02819055054491315 1103 29 -0.04908917264999246 1103 30 0.05592391122432616 1103 31 0.1169131098987819 1103 32 0.031667304563633536 1103 33 0.046664300210103704 1103 34 -0.027331796634101124 1103 35 0.1334398800811769 1103 36 0.2566969671253645 1103 37 0.13940185152893037 1103 38 0.20440252338087106 1103 39 0.16778494232290342 1103 40 0.02961377270227672 1103 41 -0.03834568862477283 1103 42 0.3889234180978747 1103 43 0.24103746515255864 1104 1 -0.1200240799961918 1104 2 -0.0747333907766432 1104 3 -0.08293713930894597 1104 4 0.017656115643752912 1104 5 -0.23374849270831533 1104 6 0.16181127315128663 1104 7 -0.1344658941080823 1104 8 0.2854598037104097 1104 9 -0.02698550806016442 1104 10 0.05086616849892778 1104 11 0.19195836692698975 1104 12 0.042567730029752227 1104 13 -0.01351144164528506 1104 14 0.12391883804698978 1104 15 0.16883154151756585 1104 16 0.08409502020441906 1104 17 0.04617334477990198 1104 18 -0.037020364062975375 1104 19 0.21234605461076939 1104 20 0.1568912086034544 1104 21 0.13802677700856947 1104 22 0.09386401181284754 1104 23 0.12020469190339611 1104 24 0.13335248946345502 1104 25 -0.11243123235289687 1104 26 -0.07131523626063796 1104 27 0.041053253610051854 1104 28 -0.03359991841755061 1104 29 0.06261749654647986 1104 30 0.03757492472715285 1104 31 0.12225732139041412 1104 32 -0.058069595793703854 1104 33 0.15195949674426856 1104 34 0.11579978480978781 1104 35 0.14982826827964146 1104 36 0.13492642037142133 1104 37 0.1666683980298984 1104 38 0.03734057479914554 1104 39 0.2443984831001913 1104 40 0.15376391658489297 1104 41 0.08637366121553214 1104 42 0.21815372515286668 1104 43 0.41390215224739535 1105 1 0.2872830066179759 1105 2 0.3279752706406853 1105 3 0.1524801835256966 1105 4 0.24797107627367154 1105 5 0.28417276713420137 1105 6 0.33505536763947674 1105 7 0.6011089090415282 1105 8 0.3256960241828537 1105 9 0.2912979565067637 1105 10 0.2555748299162488 1105 11 0.252588288186832 1105 12 0.5083157615258216 1105 13 0.33152792843345225 1105 14 0.06963537348006099 1105 15 -0.05291648997849663 1105 16 -0.0869286018428248 1105 17 -0.11004712766104774 1105 18 -0.11185500973226681 1105 19 -0.05045709499552942 1105 20 -0.05268359454615279 1105 21 -0.21957952220402888 1105 22 0.01124983858449724 1105 23 -0.3158110561280978 1105 24 -0.47028530581214056 1105 25 -0.47091942132927755 1105 26 -0.2604805905913494 1105 27 -0.3068365101694752 1105 28 -0.21676527274826185 1105 29 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0.34519205057085856 1107 14 -0.045387527283299534 1107 15 -0.05493175715406906 1107 16 -0.20211944153748362 1107 17 0.025601727300788468 1107 18 -0.12512218530478236 1107 19 -0.09337312552987953 1107 20 -0.04048512126370696 1107 21 -0.18333524739487217 1107 22 -0.34007840569030634 1107 23 -0.09350571261664697 1107 24 -0.2871860691709145 1107 25 -0.25081255110026746 1107 26 -0.03855692646921227 1107 27 -0.06602734986412884 1107 28 -0.21076527274826162 1107 29 -0.15492472861430429 1107 30 -0.22238780893876964 1107 31 0.0761853132158663 1107 32 0.10585719389193571 1107 33 -0.16617177692285878 1107 34 -0.13601845165111115 1107 35 0.023603588349367044 1107 36 -0.04031551026511693 1107 37 0.14973325347258282 1107 38 0.004296756803107371 1107 39 0.15211922602259165 1107 40 0.0780441353743413 1107 41 0.09777896265209307 1107 42 -0.03121515144747633 1107 43 -0.02056620142213461 1108 1 0.29471157804655235 1108 2 0.2540705087359214 1108 3 0.1841944692399835 1108 4 0.17416155246414888 1108 5 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2216 26 0.472107644702767 2216 27 0.4385752545364099 2216 28 0.45467590372232536 2216 29 0.32413903429543467 2216 30 0.25173657363868784 2216 31 0.0508990536738807 2216 32 0.031121001705137985 2216 33 0.18904263708702018 2216 34 0.13345842306824096 2216 35 0.056411177033695026 2216 36 0.09060613320143318 2216 37 0.32160123730734114 2216 38 0.23824377072318015 2216 39 0.35460575498532165 2216 40 0.30997049370392915 2216 41 0.4562208126745446 2216 42 0.3123524282561547 2216 43 0.5049165107467033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 20.0 26 162.0 27 733.0 28 1813.0 29 3288.0 30 5426.0 31 8546.0 32 13427.0 33 19060.0 34 28848.0 35 45868.0 36 63225.0 37 72073.0 38 43214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 97.85665917544186 1.0909709477871445 0.6097660709670877 0.4426038058039 2 1.0003336626343646 96.35260943807286 0.6035368239242651 2.043520075368501 3 1.2521957586284727 0.6539026440695708 97.31536818415259 0.778533413149364 4 0.4409522999522411 1.08569783645511 0.501141634663823 97.97220822892882 5 2.0690083807106263 1.1789258820681578 95.69678968407142 1.0552760531497996 6 2.814533250026987 94.22700106967964 1.0412202853133312 1.9172453949800292 7 96.05991416533641 1.1311595530316907 0.7451652578965273 2.063761023735378 8 1.6035591030275274 2.22967336724513 1.276435662997432 94.89033186672991 9 26.824476323639683 24.675086528984345 25.71048728015744 22.78994986721853 10 28.863122560550693 19.19104343814718 29.333917874190668 22.611916127111463 11 22.93775216704134 20.16747327397489 26.151746885645004 30.743027673338762 12 22.961511024431108 21.691525357173244 28.272151940155894 27.074811678239758 13 23.914003828596673 30.09735106922561 25.42679035978992 20.5618547423878 14 33.14039094555949 25.023395218868 16.06548043636173 25.770733399210776 15 28.29107277800859 28.47492336832601 21.966874180114694 21.267129673550706 16 21.839599341831946 35.15114639659532 25.6878543132579 17.321399948314834 17 25.933098246004278 27.394325192507736 22.38160038207143 24.290976179416557 18 18.66288521865994 33.90545725753427 22.328861672930916 25.10279585087487 19 31.49755451229287 28.074853188948683 15.91873456234767 24.50885773641078 20 26.71316226790364 25.960116718788846 23.71668215066145 23.610038862646064 21 22.93951011435899 25.946340250700022 22.77399052678408 28.34015910815691 22 24.720644806741163 24.53615261821893 24.675503101038913 26.067699474000992 23 26.309763692984063 27.947295423023576 24.185486614502917 21.55745426948944 24 25.535812419857116 27.03661057755214 26.449441289613485 20.97813571297726 25 21.056970141051476 25.730118022662445 28.786342344228405 24.426569492057677 26 21.677472962451667 23.46329239808169 33.8069129760604 21.05232166340624 27 23.082458849082773 26.431122916285034 29.646651663046608 20.839766571585585 28 21.59768926805014 25.17108052233533 30.463454845209746 22.767775364404784 29 20.964069819171485 26.031062727344096 29.427158296914662 23.577709156569753 30 24.201188077355873 25.183183733284487 28.588438489519273 22.02718969984037 31 27.367739276356463 24.583010307389852 29.269585185621338 18.779665230632343 32 23.7693817468106 28.128884527314362 29.57049394831534 18.531239777559698 33 20.661816659252086 25.69413550362442 32.53964619370372 21.104401643419777 34 19.4633353287341 26.352374690467546 32.71835735992123 21.465932620877126 35 21.827394562696472 22.3726950667805 34.45730005920779 21.34261031131523 36 20.988603354879228 28.5975976761835 29.644361866380553 20.769437102556722 37 21.3544085993366 26.426734882138582 34.478472217678885 17.740384300845932 38 20.024271844660195 24.254834742103473 30.678695731819015 25.042197681417317 39 22.278413105487658 25.225708528511237 32.87232093790071 19.623557428100387 40 23.612983002499245 23.716355024011097 31.904007955720136 20.766654017769522 41 20.30553329298811 23.88753496131765 35.40391566757716 20.40301607811708 42 18.10752575152407 24.693543725036786 34.40160552869456 22.797324994744585 43 18.436616769601173 23.364061027949333 38.358369098712444 19.840953103737046 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 7.0 7 13.0 8 27.5 9 42.0 10 135.0 11 228.0 12 228.0 13 392.0 14 556.0 15 707.0 16 858.0 17 1317.5 18 1777.0 19 1777.0 20 2901.5 21 4026.0 22 4334.0 23 4642.0 24 4589.0 25 4536.0 26 4536.0 27 6886.0 28 9236.0 29 8128.5 30 7021.0 31 8318.5 32 9616.0 33 9616.0 34 9737.0 35 9858.0 36 11534.0 37 13210.0 38 14771.0 39 16332.0 40 16332.0 41 16730.0 42 17128.0 43 18446.5 44 19765.0 45 21421.0 46 23077.0 47 23077.0 48 25968.0 49 28859.0 50 29004.5 51 29150.0 52 27921.5 53 26693.0 54 26693.0 55 28716.5 56 30740.0 57 23703.0 58 16666.0 59 16331.5 60 15997.0 61 15997.0 62 11165.5 63 6334.0 64 5185.5 65 4037.0 66 3904.0 67 3771.0 68 3771.0 69 2358.5 70 946.0 71 601.0 72 256.0 73 256.5 74 257.0 75 257.0 76 135.5 77 14.0 78 30.0 79 46.0 80 24.5 81 3.0 82 3.0 83 8.0 84 13.0 85 6.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004252479522675529 2 0.0019626828566194753 3 3.271138094365792E-4 4 6.542276188731584E-4 5 6.542276188731584E-4 6 0.001635569047182896 7 0.0 8 0.0029440242849292125 9 0.10304084997252243 10 0.016682804281265538 11 0.03369272237196766 12 0.035982519038023714 13 0.03565540522858713 14 0.028131787611545805 15 0.006869389998168163 16 0.0022897966660560543 17 6.542276188731584E-4 18 3.271138094365792E-4 19 0.012757438568026589 20 0.003925365713238951 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.005888048569858425 27 0.0 28 0.0 29 0.0 30 0.0 31 3.271138094365792E-4 32 0.0013084552377463168 33 0.0 34 3.271138094365792E-4 35 3.271138094365792E-4 36 0.0 37 0.0019626828566194753 38 0.0 39 0.0 40 0.003925365713238951 41 0.0029440242849292125 42 0.4108549446523434 43 0.0026169104754926336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 305704.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.152526627064088 #Duplication Level Percentage of deduplicated Percentage of total 1 63.894545257960466 11.598474341192787 2 11.170778296361703 4.055557009394708 3 5.548447551943489 3.021550257765682 4 3.3067233705152 2.401015361264491 5 2.1462166399365685 1.947962735194829 6 1.7011154560034598 1.8527726166487846 7 1.2902528246805904 1.6394944128961346 8 0.9766997639341899 1.4183654777170074 9 0.812715117221992 1.327754952503075 >10 7.658623610185068 30.949218852223066 >50 0.9821058511884383 11.633802632611937 >100 0.4667255329501018 15.93600345432183 >500 0.018020290847494277 2.294703373197603 >1k 0.02522840718649199 7.011357391463638 >5k 0.0018020290847494279 2.9119671316044275 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCTAGCGGCCACGTCCGACGATCTGCCGGATCTAGCTTTTT 8902 2.9119671316044275 No Hit GCTCGAGCTTGGAGATCTAGCTTTTTTTTTTTTTTTTTTTTTT 2388 0.7811477769345511 No Hit GATCTAGCGTCGACTACAGTCCGACGATCATACAGATCTAGCT 2278 0.7451652578965273 No Hit GATCTAGCAGGAAGGCCGATCTAGCTTTTTTTTTTTTTTTTTT 1913 0.625768717452176 No Hit GATCTAGCTTACTACAGTCCGACGATCAACTTGATCTAGCTTT 1876 0.6136655065030225 No Hit GATCTAGCAGGAACCGATCTAGCTTTTTTTTTTTTTTTTTTTT 1699 0.555766362232748 No Hit GATCTGGACAAATCGCTCGAACTGCGGATCGGCACGACCGTCG 1682 0.5502054274723261 No Hit GATCTAGCAGCCGCAGCCAGCTATAACCAGCGCTCTTCTGTCT 1487 0.4864182346321932 No Hit GATCTAGCTCCGGACGATCCGACCGATCTAGCTTTTTTTTTTT 1348 0.4409494151205087 No Hit GATCTAGCCAAGGCCAAGCCTCTGTGTTCAGAGCCCTTGCCCC 1264 0.4134718551278361 No Hit GATCTAGCCGCCTAGATGCTGGCAACTTTGACCAGAATAAGCT 1158 0.3787977913275587 No Hit GATCTAGCTGATAAACCCAGCAGGGTCACCATTTGCAGCGCAA 1154 0.37748933608981233 No Hit GATCTCCCCCATTAGCGCGTGCGGAGAATGAGCGCAGCCGCCG 1083 0.3542642556198153 No Hit GATCTAGCTCACGACGATCTTCTGGATCTAGCTTTTTTTTTTT 1063 0.3477219794310836 No Hit GATCTAGCTGCACTTACGGGTGGGGGGAGGGAGTGTTCTGCTG 1041 0.3405254756234789 No Hit GATCTAGCCTGAACCTCGCGGGCCGCCGGTGAAATACCACTAC 974 0.31860885039122816 No Hit GATCTAGCATTGGCATGTATGTTTGTGCAACATTTGTGTTCCT 917 0.2999633632533431 No Hit GATCTAGCCTAGAGTGGCGCACACCTGTTATCCCAGCTACTCG 762 0.24926072279067335 No Hit GATCTAGCCTTAAGATCTAGCTTTTTTTTTTTTTTTTTTTTTT 715 0.2338863737471541 No Hit GATCTAGCTCCTTGTTCTAGCTTTTTTTTTTTTTTTTTTTTTT 683 0.2234187318451836 No Hit GATCTAGCAGGAATCCGATCTAGCTTTTTTTTTTTTTTTTTTT 643 0.21033417946772043 No Hit GATCTAGCGTGTGGCGCGCTGCAACGCGACGCCGACCGGATTA 634 0.2073901551827912 No Hit GATCTAGCGTCTACAGATACAGATCTTTGGTTCGCAGAGCTGT 596 0.19495983042420117 No Hit GATCTAGCAGGAAGCCGATCTAGCTTTTTTTTTTTTTTTTTTT 581 0.1900531232826525 No Hit GATCTAGCAGGCGCAGCTGTGTGTGAAAACCTAGCATCAGAAG 510 0.16682804281265537 No Hit GATCTAGCAGACAGAGAAAATAGGAAACATGGATCTGAGGTAG 491 0.16061288043336036 No Hit GATCTAGCATAACCAGTCCGACGATCCAGGGGATCGTCGGACT 491 0.16061288043336036 No Hit GATCTAGCAGCTCTCTACAGTCCGACGATCTCCACGATCGTCG 477 0.15603328710124825 No Hit GATCTAGCTCCTTGGTGCGGTAGGCCTGGACATGGCAAAGAGA 461 0.150799466150263 No Hit GATCTAGCAGGAACCCGATCTAGCTTTTTTTTTTTTTTTTTTT 453 0.14818255567477037 No Hit GATCTAGCGACGAGTCCGACGATCGTAGTGATCGTCGGACTGT 451 0.14752832805589722 No Hit GATCTAGCGGATTCAGTCCGACGATCCTCTAGATCCTTTCGAT 449 0.14687410043702404 No Hit GATCTAGCTCCTTCAGTCCGACGATCCCTCTGATCTAGCTTTT 449 0.14687410043702404 No Hit GATCTAGCTCCTTGTGCGGTAGGCCTGGACATGGCAAAGAGAA 444 0.14523853138984114 No Hit GATCTGGGGTGCACGCACGAGGAAGGTGGCAGCGAAGCGCTGG 430 0.14065893805772903 No Hit GATCTAGCTGCACGTTACGGGTGGGGGGAGGGAGTGTTCTGCT 411 0.13444377567843405 No Hit GATCTAGCTGCACTTTACGGGTGGGGGGAGGGAGTGTTCTGCT 407 0.1331353204406877 No Hit GATCTAGCCGTGTCAAACGCCGACGTATTGCTCTGAAGAAGCA 403 0.1318268652029414 No Hit GATCTAGCGCTTGCAGTATTCCCACATGCCAGCAAACTCTCTT 400 0.13084552377463166 No Hit GATCTAGCTCTTCCAGTCCGACGATCCTTGTGATCGTCGGACT 391 0.12790149948970245 No Hit GATCTAGCCCAGCAAAGCTGATGCTTTCAGATGATGTAATCCA 391 0.12790149948970245 No Hit GATCTAGCAACCTCAGTCCGACGATCCTGAAGATCGTCGGACT 388 0.1269201580613927 No Hit GATCTAGCATCGCCTACAGTCCGACGATCTGGTAGATCTCTGA 370 0.1210321094915343 No Hit GATCTAGCAGCCGCCAGCCAGCTATAACCAGCGCTCTTCTGTC 365 0.1193965404443514 No Hit GATCTAGCTTCAACGATCAGCTGGATCTAGCAGATCGTCGGAC 364 0.11906942663491482 No Hit GATCTAGTATCGGCGTCGGGTCTCAGACCTGGGCGACACCACC 358 0.11710674377829533 No Hit GATCTAGCTCAGGCAGTCCGACGATCATGCGGATCGTCGGACT 357 0.11677962996885875 No Hit GATCTAGCACAACGATCAGTCGGACTGTAGAACTCTGAACCCC 356 0.1164525161594222 Illumina NlaIII expression Adapter 1 (95% over 24bp) GATCTAGCTCATGCAGTCCGACGATCACAGGGATCGTCGGACT 352 0.11514406092167587 No Hit GATCTAGCGCGCGCAGTCCGACGATCCCAGTGATCGTCGGACT 338 0.11056446758956377 No Hit GATCTAGCATTGGTGTTGCACATGTATGTTTGTGCAACATTTG 336 0.1099102399706906 No Hit GATCTAGCATTGGATGTTGCACATGTATGTTTGTGCAACATTT 329 0.10762044330463454 No Hit GATCTAGCGTATTGGCCCCTGAGCAAGACTGTGCGATTCAATG 322 0.1053306466385785 No Hit GAGATAGCGTACTGAACTTGGAAGGAGCCAATCAACACGTTTC 320 0.10467641901970534 No Hit GATCTAGCCACTCCAGTCCGACGATCAGCCTGATCTAAAGGAT 317 0.1036950775913956 No Hit GATCTGGCTTTGTAGTGGTGGGTCACGTCTGTAATCCCAGCAC 316 0.10336796378195902 No Hit GATCTAGCCTCACAGACCTGGAACGTCACCTCGCCGCGATCGC 312 0.10205950854421271 No Hit GGTATAGCCATGCGAAACAGAATCAGTGACGATCTTTTTCCAT 310 0.10140528092533956 No Hit GATCTAGCTCGGGGTCCGACGATCAGTTGGATCGTCGGACTGT 307 0.10042393949702981 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 3.2711380943657915E-4 0.0 0.0 12 0.0 0.0 0.0029440242849292125 0.0 0.0 13 0.0 0.0 0.02453353570774344 0.0 0.0 14 0.0 0.0 0.044814591892811347 0.0 0.0 15 0.0 0.0 0.08864784235731296 0.0 0.0 16 0.0 0.0 0.14654698662758747 0.0 0.0 17 0.0 0.0 0.16519247376547247 0.0 0.0 18 0.0 0.0 0.19724962709025723 0.0 0.0 19 0.0 0.0 0.216876455656452 0.0 0.0 20 0.0 0.0 0.23650328422264674 0.0 0.0 21 0.0 0.0 0.26169104754926337 0.0 0.0 22 0.0 0.0 0.332674744197001 0.0 0.0 23 0.0 0.0 0.524690550336273 0.0 0.0 24 0.0 0.0 0.9021798864260854 0.0 0.0 25 0.0 0.0 1.101392196372962 0.0 0.0 26 0.0 0.0 1.2881741815612489 0.0 0.0 27 0.0 0.0 1.5158453929291078 0.0 0.0 28 0.0 0.0 1.959738832334546 0.0 0.0 29 0.0 0.0 2.179559312275927 0.0 0.0 30 0.0 0.0 2.7091565697537487 0.0 0.0 31 0.0 0.0 3.0552429801376495 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTTC 20 0.001806249 37.138985 37 AGATAGT 30 8.081392E-6 37.138985 37 TGCATTC 20 0.001806249 37.138985 37 GGAGTCG 20 0.001806249 37.138985 37 TGATCCA 20 0.0018355885 37.017357 13 CGTGGTC 25 1.2281093E-4 37.017357 13 TTTCGCT 20 0.0018355885 37.017357 13 ACCCTCG 25 1.2281093E-4 37.017357 13 ATTCCGA 25 1.2281093E-4 37.017357 13 TACTGCG 20 0.0018355885 37.017357 13 CTTTACG 45 2.593879E-9 37.017357 13 GCATAAC 50 1.7826096E-10 37.0113 7 CTCACGG 25 1.2292937E-4 37.0113 9 GCGACTT 35 5.59985E-7 37.0113 7 ACGCCGG 25 1.2292937E-4 37.0113 9 GCGGTGT 25 1.2292937E-4 37.0113 7 GCGCGTA 25 1.2292937E-4 37.0113 7 GCGCGGG 45 2.5993359E-9 37.0113 7 GCGTTTG 25 1.2292937E-4 37.0113 7 CTGACGT 45 2.5993359E-9 37.0113 9 >>END_MODULE