FastQCFastQC Report
Thu 26 May 2016
SRR2099083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162816
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTAGCGGCCACGTCCGACGATCTGCCGGATCTAGCTTTTT39442.4223663522012577No Hit
GCTCGAGCTTGGAGATCTAGCTTTTTTTTTTTTTTTTTTTTTT12870.7904628537735849No Hit
GATCTAGCTTACTACAGTCCGACGATCAACTTGATCTAGCTTT10470.6430571933962264No Hit
GATCTAGCGTCGACTACAGTCCGACGATCATACAGATCTAGCT9560.5871658805031447No Hit
GATCTAGCAGGAAGGCCGATCTAGCTTTTTTTTTTTTTTTTTT8280.5085495283018868No Hit
GATCTGGACAAATCGCTCGAACTGCGGATCGGCACGACCGTCG7810.4796825864779874No Hit
GATCTAGCAGGAACCGATCTAGCTTTTTTTTTTTTTTTTTTTT7450.45757173742138363No Hit
GATCTAGCAGCCGCAGCCAGCTATAACCAGCGCTCTTCTGTCT6480.39799528301886794No Hit
GATCTAGCTGATAAACCCAGCAGGGTCACCATTTGCAGCGCAA5580.3427181603773585No Hit
GATCTAGCCAAGGCCAAGCCTCTGTGTTCAGAGCCCTTGCCCC5460.3353478773584906No Hit
GATCTAGCCGCCTAGATGCTGGCAACTTTGACCAGAATAAGCT5100.3132370283018868No Hit
GATCTAGCTGCACTTACGGGTGGGGGGAGGGAGTGTTCTGCTG4910.30156741352201255No Hit
GATCTCCCCCATTAGCGCGTGCGGAGAATGAGCGCAGCCGCCG4540.27884237421383645No Hit
GATCTAGCCTGAACCTCGCGGGCCGCCGGTGAAATACCACTAC4250.261030856918239No Hit
GATCTAGCATTGGCATGTATGTTTGTGCAACATTTGTGTTCCT4180.2567315251572327No Hit
GATCTAGCTCACGACGATCTTCTGGATCTAGCTTTTTTTTTTT4000.2456761006289308No Hit
GATCTAGCTCCGGACGATCCGACCGATCTAGCTTTTTTTTTTT3850.23646324685534592No Hit
GATCTAGCCTAGAGTGGCGCACACCTGTTATCCCAGCTACTCG3540.21742334905660377No Hit
GATCTAGCGTGTGGCGCGCTGCAACGCGACGCCGACCGGATTA3100.1903989779874214No Hit
GATCTAGCCTTAAGATCTAGCTTTTTTTTTTTTTTTTTTTTTT2880.1768867924528302No Hit
GATCTAGCGTCTACAGATACAGATCTTTGGTTCGCAGAGCTGT2840.17443003144654087No Hit
GATCTAGCAGGAATCCGATCTAGCTTTTTTTTTTTTTTTTTTT2750.16890231918238993No Hit
GATCTAGCTCCTTGTTCTAGCTTTTTTTTTTTTTTTTTTTTTT2610.16030365566037735No Hit
GATCTAGCAGGAAGCCGATCTAGCTTTTTTTTTTTTTTTTTTT2600.15968946540880505No Hit
GATCTAGCAGACAGAGAAAATAGGAAACATGGATCTGAGGTAG2410.14801985062893083No Hit
GATCTAGCGGATTCAGTCCGACGATCCTCTAGATCCTTTCGAT2350.14433470911949683No Hit
GATCTAGCTTTACCTACAGTCCGACGATCGGGGCGATCTAGCT2330.1431063286163522No Hit
GATCTAGCAGCTCTCTACAGTCCGACGATCTCCACGATCGTCG2310.14187794811320753No Hit
GATCTAGCATAACCAGTCCGACGATCCAGGGGATCGTCGGACT2220.1363502358490566No Hit
GATCTAGCGACGAGTCCGACGATCGTAGTGATCGTCGGACTGT2200.13512185534591195No Hit
GATCTAGCAGGCGCAGCTGTGTGTGAAAACCTAGCATCAGAAG2120.13020833333333331No Hit
GATCTAGCTCCTTCAGTCCGACGATCCCTCTGATCTAGCTTTT2090.12836576257861634No Hit
GATCTAGCTCCTTGGTGCGGTAGGCCTGGACATGGCAAAGAGA2080.127751572327044No Hit
GATCTAGCTCCTTGTGCGGTAGGCCTGGACATGGCAAAGAGAA2070.1271373820754717No Hit
GATCTGGGGTGCACGCACGAGGAAGGTGGCAGCGAAGCGCTGG2070.1271373820754717No Hit
GATCTAGCAGGAACCCGATCTAGCTTTTTTTTTTTTTTTTTTT2010.12345224056603774No Hit
GATCTAGCGCTTGCAGTATTCCCACATGCCAGCAAACTCTCTT1910.11731033805031446No Hit
GATCTAGCAACCTCAGTCCGACGATCCTGAAGATCGTCGGACT1910.11731033805031446No Hit
GATCTAGTATCGGCGTCGGGTCTCAGACCTGGGCGACACCACC1840.11301100628930817No Hit
GATCTAGCCGTGTCAAACGCCGACGTATTGCTCTGAAGAAGCA1830.11239681603773585No Hit
GATCTAGCTGCACGTTACGGGTGGGGGGAGGGAGTGTTCTGCT1810.11116843553459119No Hit
GATCTAGCATGCGGACAAGGATTATTTCTTATTTAGAGATAGT1810.11116843553459119No Hit
GATCTAGCTGCACTTTACGGGTGGGGGGAGGGAGTGTTCTGCT1810.11116843553459119No Hit
GATCTAGCATCGCCTACAGTCCGACGATCTGGTAGATCTCTGA1780.10932586477987422No Hit
GATCTAGCTCTTCCAGTCCGACGATCCTTGTGATCGTCGGACT1770.10871167452830188No Hit
GATCTAGCAGCCGCCAGCCAGCTATAACCAGCGCTCTTCTGTC1740.1068691037735849No Hit
GATCTAGCACAACGATCAGTCGGACTGTAGAACTCTGAACCCC1660.10195558176100629Illumina NlaIII expression Adapter 1 (95% over 24bp)
GATCTAGCATTGGTGTTGCACATGTATGTTTGTGCAACATTTG1650.10134139150943396No Hit
GATCTAGCAACTTGGAGAAGATTGCTTTCAACGGGTCTGCCTT1640.10072720125786164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTG200.001835276637.00829718
TTGTCAC200.001835276637.00829735
ACTTAAG200.001835276637.00829718
GACTTAA200.001835276637.00829717
CCACTAC551.2732926E-1137.00829737
CGCACAC403.7913196E-837.00829718
GGTCTTA200.001835276637.00829736
GTCTTAC200.001835276637.00829737
CGGAGCC200.001835276637.00829713
TTTCGAT452.584784E-937.00829337
AGAAGTA251.2273545E-437.00829316
AAGTTGG251.2273545E-437.00829313
GAGCGTC251.2273545E-437.00829317
GTATCCT251.2273545E-437.00829337
GGACAGG251.2273545E-437.00829314
TGTCCGA452.584784E-937.00829313
GCATAAC403.801506E-836.996937
AGCGTTA501.7826096E-1036.996936
CATGGCG251.2295769E-436.996938
AGCGTGG308.272909E-636.996936
Produced by FastQC (version 0.11.5)