##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099083_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 162816 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.991972533411946 37.0 35.0 37.0 31.0 37.0 2 33.91026680424528 35.0 33.0 35.0 31.0 37.0 3 34.213136301100626 35.0 35.0 37.0 31.0 37.0 4 37.400869693396224 39.0 37.0 39.0 34.0 39.0 5 36.76642958922956 39.0 37.0 39.0 32.0 39.0 6 36.186277761399374 37.0 35.0 39.0 32.0 39.0 7 36.26278129913522 39.0 37.0 39.0 30.0 39.0 8 36.96185264347484 39.0 37.0 39.0 33.0 39.0 9 31.003709709119498 33.0 31.0 34.0 27.0 34.0 10 31.114165683962263 33.0 31.0 34.0 27.0 34.0 11 31.36363748034591 33.0 31.0 34.0 28.0 34.0 12 34.89037318199686 37.0 35.0 37.0 32.0 37.0 13 35.039388020833336 37.0 35.0 37.0 32.0 37.0 14 31.859983048349058 31.0 31.0 34.0 30.0 34.0 15 31.845205630896228 31.0 31.0 34.0 30.0 34.0 16 31.924712558962263 31.0 31.0 34.0 30.0 34.0 17 35.1936726120283 35.0 35.0 37.0 32.0 37.0 18 35.43189244300314 37.0 35.0 37.0 33.0 37.0 19 34.655746363993714 36.0 35.0 37.0 30.0 37.0 20 34.026060092374216 35.0 33.0 37.0 28.0 37.0 21 34.01231451454402 35.0 33.0 37.0 28.0 37.0 22 35.516018081761004 38.0 34.0 39.0 28.0 39.0 23 35.89174896816038 38.0 35.0 39.0 30.0 39.0 24 36.272411802279876 39.0 35.0 39.0 31.0 39.0 25 36.52303213443396 39.0 35.0 39.0 32.0 39.0 26 36.56724154874214 39.0 35.0 39.0 32.0 39.0 27 38.034449931210695 40.0 37.0 41.0 33.0 41.0 28 38.19563187893082 40.0 37.0 41.0 33.0 41.0 29 38.43698408018868 40.0 37.0 41.0 34.0 41.0 30 38.520520096305034 40.0 37.0 41.0 35.0 41.0 31 38.596698113207545 40.0 38.0 41.0 35.0 41.0 32 38.77567929441824 40.0 38.0 41.0 35.0 41.0 33 38.855481033805034 40.0 38.0 41.0 35.0 41.0 34 38.929644506682386 40.0 38.0 41.0 35.0 41.0 35 38.932138119103776 40.0 38.0 41.0 35.0 41.0 36 38.96317315251572 40.0 38.0 41.0 36.0 41.0 37 38.833192069575475 40.0 38.0 41.0 35.0 41.0 38 38.727354805424525 40.0 38.0 41.0 35.0 41.0 39 38.6195705581761 40.0 38.0 41.0 35.0 41.0 40 38.61250737028302 40.0 38.0 41.0 35.0 41.0 41 38.46975727201258 40.0 38.0 41.0 35.0 41.0 42 38.47556136988994 40.0 38.0 41.0 35.0 41.0 43 38.40401434748428 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6015848511039366 1101 2 0.6097370846622496 1101 3 0.7629073552995749 1101 4 0.3493635845066123 1101 5 0.569768513832976 1101 6 0.7287759672896854 1101 7 0.5376321078071129 1101 8 0.6360140678436963 1101 9 1.0566423180478886 1101 10 1.0118420469188045 1101 11 0.9705588615922913 1101 12 0.8225004364791602 1101 13 0.4996150944026141 1101 14 0.040702793878136134 1101 15 0.0608305600688297 1101 16 -0.09914488118067766 1101 17 -0.09034911332086892 1101 18 -0.5153591584385708 1101 19 -0.44678251843883743 1101 20 -0.5435542411339398 1101 21 -0.1292848541485654 1101 22 -0.07831569503675695 1101 23 -0.13341706103989281 1101 24 -0.4523475434852884 1101 25 -0.06393917533526405 1101 26 -0.24569865744967245 1101 27 -0.41826414328949824 1101 28 -0.3632377053171325 1101 29 -0.23168144210811903 1101 30 -0.17089412176298424 1101 31 -0.09454287008741602 1101 32 0.03035925962716135 1101 33 0.1923797046048037 1101 34 0.18025323916439362 1101 35 0.12795903397404373 1101 36 0.07992462589404425 1101 37 0.06719658392223948 1101 38 0.4096000050243944 1101 39 0.041249008473087656 1101 40 0.15370264250622512 1101 41 0.09487139502634534 1101 42 0.19935228966481588 1101 43 0.11203506035502642 1102 1 0.2514776207117819 1102 2 0.8692499523093105 1102 3 0.5713938749074146 1102 4 0.4282391972517132 1102 5 0.4392691265780755 1102 6 0.5726485163092931 1102 7 0.5186829656502496 1102 8 0.3835784060789891 1102 9 0.6148837396165128 1102 10 0.804136777310962 1102 11 0.5939963615922927 1102 12 0.5383398972634765 1102 13 0.47789328067712233 1102 14 0.15035352917225353 1102 15 0.22120127085314323 1102 16 -0.0517030919649919 1102 17 0.2020834847183437 1102 18 -0.09311651137974764 1102 19 -0.19762504294864414 1102 20 -0.15899541760452962 1102 21 0.18753499879261426 1102 22 -0.12724339111518645 1102 23 -0.49979267378498804 1102 24 -0.12353320524999134 1102 25 0.031909476625521904 1102 26 -0.25099890254771395 1102 27 -0.18241855505420546 1102 28 -0.4642487347288977 1102 29 -0.12722372151988282 1102 30 -0.3715375041159277 1102 31 0.03617097795179802 1102 32 -0.08624613252970192 1102 33 -0.11647446206186629 1102 34 -0.3085182069140373 1102 35 0.2095766810328641 1102 36 0.03671077785482879 1102 37 0.24711386333400753 1102 38 0.0964259854165519 1102 39 0.44069753788485144 1102 40 0.11928168662387151 1102 41 0.29176171365379844 1102 42 0.2707217749589361 1102 43 0.2973598152569892 1103 1 0.345781380290326 1103 2 0.6427323517346011 1103 3 0.44043721781929435 1103 4 0.18903002579577333 1103 5 0.1312740355819102 1103 6 0.2773898956196348 1103 7 0.4759237463015893 1103 8 0.5182317825157696 1103 9 0.10437727409927078 1103 10 0.08067233765579118 1103 11 0.1950799343892271 1103 12 0.2574790256159645 1103 13 0.3066468726311484 1103 14 -0.1032683578009248 1103 15 -0.2597464063499224 1103 16 -0.32186293391901444 1103 17 -0.56476876623951 1103 18 0.07684277980798981 1103 19 -0.559020517086573 1103 20 -0.8129705133899705 1103 21 -0.9084599724717535 1103 22 -1.113384435176492 1103 23 -0.8451141536700462 1103 24 -1.0115091391580364 1103 25 -0.9059946182020653 1103 26 -0.29221657496150755 1103 27 -0.5283835933683818 1103 28 -0.3063524224683647 1103 29 -0.33341684891451706 1103 30 -0.27889502327301585 1103 31 -0.2639816800941759 1103 32 -0.03518051950288026 1103 33 0.010770509202501444 1103 34 0.050931625461444696 1103 35 -0.06987734195563888 1103 36 0.005685274981267696 1103 37 0.256288312567726 1103 38 0.1872425562977753 1103 39 0.31539820838293764 1103 40 0.25764674888440453 1103 41 0.3263791705503536 1103 42 0.2975267318554913 1103 43 0.30229875203859535 1104 1 -0.014238222311476534 1104 2 0.20782189998372758 1104 3 -0.21415602819335788 1104 4 -0.02437102561650306 1104 5 -0.09388857691029528 1104 6 -0.13520032090001166 1104 7 -0.02652657892339505 1104 8 -0.08053859973496458 1104 9 0.4470663946552733 1104 10 0.339293027310962 1104 11 0.24885343523570214 1104 12 0.3177124747828586 1104 13 0.16363698222750855 1104 14 -0.1328950173393757 1104 15 -0.0664380217825169 1104 16 -0.17110110553088376 1104 17 -0.1849683563669302 1104 18 -0.10341205401540776 1104 19 -0.47642228132073683 1104 20 -0.42207681295336386 1104 21 -1.0660381601221118 1104 22 -0.31310821669658395 1104 23 -0.39844214083925067 1104 24 -0.6324661025368101 1104 25 -0.36776954469230816 1104 26 -0.06380171262523504 1104 27 -0.2007082837363754 1104 28 -0.35772014945757746 1104 29 -0.21918714206251622 1104 30 -0.3185457405500358 1104 31 -0.027146609257506782 1104 32 -0.11519477594055161 1104 33 0.08307737902340762 1104 34 -0.05764005768923397 1104 35 -0.1445899856338002 1104 36 0.1179547216532768 1104 37 0.15702786527199208 1104 38 0.33395021022275273 1104 39 0.27294075493911407 1104 40 0.11713173507348529 1104 41 0.17708753729721138 1104 42 0.19474672650932234 1104 43 0.12881572610969982 1105 1 -0.07625372618744564 1105 2 0.5411552333170633 1105 3 -0.11338083439490987 1105 4 0.1500475790346556 1105 5 0.00688661688815273 1105 6 0.34541983413875244 1105 7 -0.02265060993114787 1105 8 0.18690326073015484 1105 9 0.03233771248472905 1105 10 0.12611473273731733 1105 11 0.35738056701864807 1105 12 0.1432938701316928 1105 13 0.09386954036704509 1105 14 0.04152358731179007 1105 15 -0.054810114805771804 1105 16 -0.016062345840961 1105 17 0.1677448219276485 1105 18 -0.0026368602169597466 1105 19 -0.20510445186337023 1105 20 -0.16626285946499308 1105 21 -0.3179761446182354 1105 22 -0.2782244957663522 1105 23 -0.5612328385136678 1105 24 -0.6324661025368101 1105 25 0.19424595918366094 1105 26 0.29278743466159085 1105 27 -0.09218115195343302 1105 28 -0.0166348781397474 1105 29 0.0676345633638391 1105 30 0.23571782534143892 1105 31 0.06200067756419969 1105 32 -0.10744283795605725 1105 33 -0.13397688454248424 1105 34 0.18267001983014808 1105 35 0.05696040196309582 1105 36 0.141210535606767 1105 37 0.21129143116346683 1105 38 0.4037176520832162 1105 39 0.11015005726469695 1105 40 0.132635611042474 1105 41 -0.03221478828418611 1105 42 -0.10370288589377452 1105 43 0.02416456331900463 1106 1 0.19932471040643662 1106 2 0.01068005727114496 1106 3 0.25072355047993256 1106 4 -0.2714566519849271 1106 5 0.001620033066622284 1106 6 0.46625538653830034 1106 7 0.28207156297849423 1106 8 0.2529426713461618 1106 9 0.25270578160124657 1106 10 0.06830066089874975 1106 11 0.06674206769916324 1106 12 0.15755506901920313 1106 13 -0.04971883764348917 1106 14 -0.06046469601858462 1106 15 -0.04457282265830642 1106 16 -0.4540468419649919 1106 17 -0.1818024885641023 1106 18 0.059674519154604866 1106 19 0.17340906964677316 1106 20 -0.12702976874956562 1106 21 0.08346772780024736 1106 22 -0.05037077661136635 1106 23 -0.06297850393765714 1106 24 -0.04686932166220714 1106 25 0.087819543419414 1106 26 0.14553020050571774 1106 27 0.038359116701521145 1106 28 0.15961815458408068 1106 29 -0.024856116558048313 1106 30 0.06745343099857593 1106 31 0.18881291497469732 1106 32 0.23663077586724768 1106 33 0.1982321219076013 1106 34 0.031862279727185694 1106 35 -0.012274464005301411 1106 36 -0.009538029397077707 1106 37 -0.06481958132248167 1106 38 -0.0759261138200884 1106 39 -0.22664501936705506 1106 40 -0.4824895442921573 1106 41 -0.32563451726223036 1106 42 -0.17891443687312147 1106 43 -0.21582005115522662 1107 1 -0.483230470373492 1107 2 -0.2727982550550294 1107 3 -0.6482645553251416 1107 4 -0.28018497910487383 1107 5 -0.3613304373754147 1107 6 -0.09256466198527846 1107 7 -0.25520874946602845 1107 8 -0.15418201058767522 1107 9 -0.018049884414494954 1107 10 -0.09481549982082171 1107 11 0.04342707864655537 1107 12 0.1859295290464189 1107 13 -0.057293250330630485 1107 14 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0.07488700976789886 1108 8 0.23601406784369772 1108 9 0.14291682785180626 1108 10 0.26674400770311735 1108 11 0.1078137635530787 1108 12 0.4303435737340635 1108 13 0.3623601924418267 1108 14 -0.292630539455196 1108 15 -0.33132630267626695 1108 16 -0.02855664588656026 1108 17 -0.18446676037969212 1108 18 -0.06437876628170613 1108 19 0.04341355999252983 1108 20 0.16624968043468868 1108 21 -0.4469319129720901 1108 22 -0.6469431460171506 1108 23 -0.2942013747653789 1108 24 -0.32685734740685746 1108 25 -0.39727250866859265 1108 26 -0.3319731672535937 1108 27 -0.3163033589757731 1108 28 0.013232882918160271 1108 29 -0.21599516759831516 1108 30 -0.19050196490024263 1108 31 -0.22395463479330147 1108 32 -0.13826819135323376 1108 33 -0.12918892284618266 1108 34 -0.1570016627963895 1108 35 -0.013217436614198164 1108 36 -0.1710557662628176 1108 37 -0.15241125921501464 1108 38 -0.22177254399521473 1108 39 -0.17051569740926453 1108 40 -0.41100323984671405 1108 41 -0.2502266441893397 1108 42 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0.8148327929102592 2216 22 0.6094294030024585 2216 23 0.846484899744425 2216 24 0.6432406918088347 2216 25 0.5137569030961089 2216 26 0.298173891569931 2216 27 0.5207064449457945 2216 28 0.4249975888005153 2216 29 0.23375973436247222 2216 30 0.1776352900017173 2216 31 0.011584580892971985 2216 32 0.08011416158794304 2216 33 0.15144833205577868 2216 34 0.047410101909491686 2216 35 -0.0036586130847808818 2216 36 -0.2254675309687002 2216 37 -0.0982445925483475 2216 38 -0.3585372498775641 2216 39 -0.2881627562327935 2216 40 -0.20217971043495453 2216 41 -0.04900115399325955 2216 42 -0.1146182985704769 2216 43 -0.14703356709595283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 6.0 26 34.0 27 141.0 28 369.0 29 793.0 30 1577.0 31 2964.0 32 5804.0 33 9220.0 34 15600.0 35 26517.0 36 34868.0 37 31955.0 38 32968.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 98.5615575960446 0.7173786199060282 0.40966741393606243 0.3113963701133188 2 0.3820380686808631 97.6438938400968 0.39002278715811584 1.5840453040642215 3 0.8592521619496856 0.5509286556603774 97.95228970125787 0.6375294811320754 4 0.30095692016657044 0.4551205670274055 0.23155256918938175 99.01236994361665 5 1.6896373820754718 0.9446246069182389 96.49911556603774 0.8666224449685533 6 2.6017738250251825 94.92420706090459 0.9968552686534161 1.4771638454167997 7 96.68828616352201 0.7689661949685535 0.6694673742138365 1.8732802672955975 8 1.3881467013089117 1.871541149950555 1.0011854527139497 95.73912669602659 9 26.706758234332963 23.946214233939823 26.49729105495289 22.84973647677433 10 27.685985946636528 19.35531423517272 30.42172374821876 22.536976069971992 11 23.712872807691127 20.284424240562707 26.635746536843076 29.366956414903093 12 22.75069873153352 21.56822998249332 28.796953223379095 26.88411806259406 13 22.993814838246042 28.78649214724002 26.445098243954572 21.774594770559368 14 38.58927024652754 26.114841412695583 13.49051793513985 21.805370405637024 15 28.732702335837256 29.8444208315163 22.5856975265799 18.837179306066542 16 19.855664404385344 42.063077726253724 22.68157110831312 15.399686761047814 17 30.09612136473912 29.060590240456964 19.686761047815004 21.156527346988913 18 17.16917974388109 43.76869453060222 18.30113932991432 20.76098639560237 19 34.97490647517369 29.929172988678737 13.328746675184746 21.767173860962828 20 27.19404928625832 27.056460529225323 22.403626446524655 23.345863737991696 21 22.819010416666664 26.513364779874216 22.50270243710692 28.1649223663522 22 24.50373427672956 24.78871855345912 24.439244300314467 26.268302869496857 23 26.399739583333332 28.15878046383648 24.193568199685533 21.247911753144656 24 25.62217472484277 27.94319968553459 25.595764544025158 20.838861045597483 25 21.061197916666664 26.13809453616352 28.183962264150942 24.61674528301887 26 22.02806866689187 23.640942173632652 33.21192764794398 21.11906151153149 27 22.902540290880502 27.13553950471698 29.003292059748425 20.95862814465409 28 21.900181800314467 25.304638364779876 30.670818592767297 22.124361242138367 29 22.00705090408805 25.54110161163522 28.82394850628931 23.62789897798742 30 23.62544221698113 25.339033018867923 29.009433962264154 22.02609080188679 31 26.74139360624021 24.880385713847 29.13675029941959 19.241470380493197 32 23.857578065498856 27.70925975972287 29.475714320811736 18.95744785396654 33 21.098049331761008 25.586551690251575 31.735824488993707 21.57957448899371 34 20.155391088044713 26.22178546202745 32.01363510733041 21.609188342597427 35 22.4481773792341 23.15757147682953 33.300371587384454 21.093879556551915 36 21.54272307389937 28.007689661949687 29.447965801886795 21.00162146226415 37 21.507278422701308 26.274798845279772 33.64166820219888 18.576254529820034 38 21.19631977201258 24.483465998427672 29.92764838836478 24.39256584119497 39 22.470150353773587 25.355001965408807 32.00176886792453 20.17307881289308 40 23.289354069924574 23.923859364634776 31.511510773691064 21.275275791749586 41 21.035950542667084 24.256942269067057 34.24668472485827 20.460422463407593 42 19.40165585691824 24.673864976415093 33.402122641509436 22.52235652515723 43 19.360227998624147 23.750061422043142 36.44599773966881 20.4437128396639 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 7.0 7 13.0 8 24.5 9 36.0 10 102.0 11 168.0 12 168.0 13 274.5 14 381.0 15 426.0 16 471.0 17 723.5 18 976.0 19 976.0 20 1434.5 21 1893.0 22 2001.0 23 2109.0 24 2208.0 25 2307.0 26 2307.0 27 3430.0 28 4553.0 29 4139.0 30 3725.0 31 4304.5 32 4884.0 33 4884.0 34 5148.5 35 5413.0 36 6220.5 37 7028.0 38 7941.0 39 8854.0 40 8854.0 41 9298.5 42 9743.0 43 10309.5 44 10876.0 45 12134.5 46 13393.0 47 13393.0 48 14340.5 49 15288.0 50 15468.0 51 15648.0 52 14905.0 53 14162.0 54 14162.0 55 14972.0 56 15782.0 57 12415.5 58 9049.0 59 8650.5 60 8252.0 61 8252.0 62 5810.0 63 3368.0 64 2720.5 65 2073.0 66 1878.0 67 1683.0 68 1683.0 69 1065.0 70 447.0 71 271.5 72 96.0 73 103.5 74 111.0 75 111.0 76 58.0 77 5.0 78 12.5 79 20.0 80 10.0 81 0.0 82 0.0 83 4.0 84 8.0 85 4.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.141902515723271E-4 2 0.0030709512578616354 3 0.0 4 0.0012283805031446542 5 0.0 6 0.0024567610062893085 7 0.0 8 0.005527712264150944 9 0.013512185534591195 10 0.004913522012578617 11 0.01903989779874214 12 0.012897995283018868 13 0.004299331761006289 14 0.021496658805031446 15 0.0030709512578616354 16 6.141902515723271E-4 17 6.141902515723271E-4 18 6.141902515723271E-4 19 0.015354756289308175 20 0.007370283018867925 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 6.141902515723271E-4 27 0.0 28 0.0 29 0.0 30 0.0 31 6.141902515723271E-4 32 0.0024567610062893085 33 0.0 34 6.141902515723271E-4 35 6.141902515723271E-4 36 0.0 37 0.0036851415094339623 38 0.0 39 0.0 40 0.007370283018867925 41 0.005527712264150944 42 0.0 43 0.004913522012578617 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 162816.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.545990566037734 #Duplication Level Percentage of deduplicated Percentage of total 1 54.97423328305682 10.745258451257863 2 12.851935646053292 5.024076257861635 3 6.840749120160885 4.011276533018868 4 4.11639014580191 3.2183569182389937 5 2.9568878833584713 2.8897651336477987 6 2.381850175967823 2.7933372641509435 7 1.9073655103066869 2.6096943789308176 8 1.4234539969834088 2.2258254716981134 9 1.3417546505781799 2.3603331367924527 >10 10.221845148315737 37.58721501572327 >50 0.628456510809452 8.47214033018868 >100 0.3205128205128205 10.78456662735849 >500 0.025138260432378077 3.4222680817610063 >1k 0.009426847662141781 3.855886399371069 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCTAGCGGCCACGTCCGACGATCTGCCGGATCTAGCTTTTT 3944 2.4223663522012577 No Hit GCTCGAGCTTGGAGATCTAGCTTTTTTTTTTTTTTTTTTTTTT 1287 0.7904628537735849 No Hit GATCTAGCTTACTACAGTCCGACGATCAACTTGATCTAGCTTT 1047 0.6430571933962264 No Hit GATCTAGCGTCGACTACAGTCCGACGATCATACAGATCTAGCT 956 0.5871658805031447 No Hit GATCTAGCAGGAAGGCCGATCTAGCTTTTTTTTTTTTTTTTTT 828 0.5085495283018868 No Hit GATCTGGACAAATCGCTCGAACTGCGGATCGGCACGACCGTCG 781 0.4796825864779874 No Hit GATCTAGCAGGAACCGATCTAGCTTTTTTTTTTTTTTTTTTTT 745 0.45757173742138363 No Hit GATCTAGCAGCCGCAGCCAGCTATAACCAGCGCTCTTCTGTCT 648 0.39799528301886794 No Hit GATCTAGCTGATAAACCCAGCAGGGTCACCATTTGCAGCGCAA 558 0.3427181603773585 No Hit GATCTAGCCAAGGCCAAGCCTCTGTGTTCAGAGCCCTTGCCCC 546 0.3353478773584906 No Hit GATCTAGCCGCCTAGATGCTGGCAACTTTGACCAGAATAAGCT 510 0.3132370283018868 No Hit GATCTAGCTGCACTTACGGGTGGGGGGAGGGAGTGTTCTGCTG 491 0.30156741352201255 No Hit GATCTCCCCCATTAGCGCGTGCGGAGAATGAGCGCAGCCGCCG 454 0.27884237421383645 No Hit GATCTAGCCTGAACCTCGCGGGCCGCCGGTGAAATACCACTAC 425 0.261030856918239 No Hit GATCTAGCATTGGCATGTATGTTTGTGCAACATTTGTGTTCCT 418 0.2567315251572327 No Hit GATCTAGCTCACGACGATCTTCTGGATCTAGCTTTTTTTTTTT 400 0.2456761006289308 No Hit GATCTAGCTCCGGACGATCCGACCGATCTAGCTTTTTTTTTTT 385 0.23646324685534592 No Hit GATCTAGCCTAGAGTGGCGCACACCTGTTATCCCAGCTACTCG 354 0.21742334905660377 No Hit GATCTAGCGTGTGGCGCGCTGCAACGCGACGCCGACCGGATTA 310 0.1903989779874214 No Hit GATCTAGCCTTAAGATCTAGCTTTTTTTTTTTTTTTTTTTTTT 288 0.1768867924528302 No Hit GATCTAGCGTCTACAGATACAGATCTTTGGTTCGCAGAGCTGT 284 0.17443003144654087 No Hit GATCTAGCAGGAATCCGATCTAGCTTTTTTTTTTTTTTTTTTT 275 0.16890231918238993 No Hit GATCTAGCTCCTTGTTCTAGCTTTTTTTTTTTTTTTTTTTTTT 261 0.16030365566037735 No Hit GATCTAGCAGGAAGCCGATCTAGCTTTTTTTTTTTTTTTTTTT 260 0.15968946540880505 No Hit GATCTAGCAGACAGAGAAAATAGGAAACATGGATCTGAGGTAG 241 0.14801985062893083 No Hit GATCTAGCGGATTCAGTCCGACGATCCTCTAGATCCTTTCGAT 235 0.14433470911949683 No Hit GATCTAGCTTTACCTACAGTCCGACGATCGGGGCGATCTAGCT 233 0.1431063286163522 No Hit GATCTAGCAGCTCTCTACAGTCCGACGATCTCCACGATCGTCG 231 0.14187794811320753 No Hit GATCTAGCATAACCAGTCCGACGATCCAGGGGATCGTCGGACT 222 0.1363502358490566 No Hit GATCTAGCGACGAGTCCGACGATCGTAGTGATCGTCGGACTGT 220 0.13512185534591195 No Hit GATCTAGCAGGCGCAGCTGTGTGTGAAAACCTAGCATCAGAAG 212 0.13020833333333331 No Hit GATCTAGCTCCTTCAGTCCGACGATCCCTCTGATCTAGCTTTT 209 0.12836576257861634 No Hit GATCTAGCTCCTTGGTGCGGTAGGCCTGGACATGGCAAAGAGA 208 0.127751572327044 No Hit GATCTAGCTCCTTGTGCGGTAGGCCTGGACATGGCAAAGAGAA 207 0.1271373820754717 No Hit GATCTGGGGTGCACGCACGAGGAAGGTGGCAGCGAAGCGCTGG 207 0.1271373820754717 No Hit GATCTAGCAGGAACCCGATCTAGCTTTTTTTTTTTTTTTTTTT 201 0.12345224056603774 No Hit GATCTAGCGCTTGCAGTATTCCCACATGCCAGCAAACTCTCTT 191 0.11731033805031446 No Hit GATCTAGCAACCTCAGTCCGACGATCCTGAAGATCGTCGGACT 191 0.11731033805031446 No Hit GATCTAGTATCGGCGTCGGGTCTCAGACCTGGGCGACACCACC 184 0.11301100628930817 No Hit GATCTAGCCGTGTCAAACGCCGACGTATTGCTCTGAAGAAGCA 183 0.11239681603773585 No Hit GATCTAGCTGCACGTTACGGGTGGGGGGAGGGAGTGTTCTGCT 181 0.11116843553459119 No Hit GATCTAGCATGCGGACAAGGATTATTTCTTATTTAGAGATAGT 181 0.11116843553459119 No Hit GATCTAGCTGCACTTTACGGGTGGGGGGAGGGAGTGTTCTGCT 181 0.11116843553459119 No Hit GATCTAGCATCGCCTACAGTCCGACGATCTGGTAGATCTCTGA 178 0.10932586477987422 No Hit GATCTAGCTCTTCCAGTCCGACGATCCTTGTGATCGTCGGACT 177 0.10871167452830188 No Hit GATCTAGCAGCCGCCAGCCAGCTATAACCAGCGCTCTTCTGTC 174 0.1068691037735849 No Hit GATCTAGCACAACGATCAGTCGGACTGTAGAACTCTGAACCCC 166 0.10195558176100629 Illumina NlaIII expression Adapter 1 (95% over 24bp) GATCTAGCATTGGTGTTGCACATGTATGTTTGTGCAACATTTG 165 0.10134139150943396 No Hit GATCTAGCAACTTGGAGAAGATTGCTTTCAACGGGTCTGCCTT 164 0.10072720125786164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.002456761006289308 0.0 0.0 13 0.0 0.0 0.017811517295597483 0.0 0.0 14 0.0 0.0 0.027638561320754717 0.0 0.0 15 0.0 0.0 0.06756092767295598 0.0 0.0 16 0.0 0.0 0.10809748427672956 0.0 0.0 17 0.0 0.0 0.12222386006289308 0.0 0.0 18 0.0 0.0 0.1498624213836478 0.0 0.0 19 0.0 0.0 0.1547759433962264 0.0 0.0 20 0.0 0.0 0.15846108490566038 0.0 0.0 21 0.0 0.0 0.1793435534591195 0.0 0.0 22 0.0 0.0 0.2155807783018868 0.0 0.0 23 0.0 0.0 0.38816823899371067 0.0 0.0 24 0.0 0.0 0.7290438286163522 0.0 0.0 25 0.0 0.0 0.8653940644654088 0.0 0.0 26 0.0 0.0 1.0201700078616351 0.0 0.0 27 0.0 0.0 1.2197818396226414 0.0 0.0 28 0.0 0.0 1.5864534198113207 0.0 0.0 29 0.0 0.0 1.7793091588050314 0.0 0.0 30 0.0 0.0 2.2626768867924527 0.0 0.0 31 0.0 0.0 2.5458185927672954 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACTTG 20 0.0018352766 37.008297 18 TTGTCAC 20 0.0018352766 37.008297 35 ACTTAAG 20 0.0018352766 37.008297 18 GACTTAA 20 0.0018352766 37.008297 17 CCACTAC 55 1.2732926E-11 37.008297 37 CGCACAC 40 3.7913196E-8 37.008297 18 GGTCTTA 20 0.0018352766 37.008297 36 GTCTTAC 20 0.0018352766 37.008297 37 CGGAGCC 20 0.0018352766 37.008297 13 TTTCGAT 45 2.584784E-9 37.008293 37 AGAAGTA 25 1.2273545E-4 37.008293 16 AAGTTGG 25 1.2273545E-4 37.008293 13 GAGCGTC 25 1.2273545E-4 37.008293 17 GTATCCT 25 1.2273545E-4 37.008293 37 GGACAGG 25 1.2273545E-4 37.008293 14 TGTCCGA 45 2.584784E-9 37.008293 13 GCATAAC 40 3.801506E-8 36.99693 7 AGCGTTA 50 1.7826096E-10 36.99693 6 CATGGCG 25 1.2295769E-4 36.99693 8 AGCGTGG 30 8.272909E-6 36.99693 6 >>END_MODULE