FastQCFastQC Report
Thu 26 May 2016
SRR2099082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381597
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTCATAAAACGACTCACTATAGGGAGTCGTATTACCGG82412.159608173020228No Hit
TGCACGTCATCCACGACTCACTATAGTGAGTCGTATTACCGGC80252.1030039544336043No Hit
TGCACGTCATAAAACGACTCACTATAGTGAGTCGTATTACCGG62901.6483358097678966No Hit
TGCACGTAAAAGAGTCGGACTGTAGAACTCTGAACCCCTATAG48831.2796222192522477Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGCACGTATAACGCAGTCCGACGATCTACATTGCACGTATTTT29630.7764736095933669No Hit
TGCACGTAAAAGAGGTCGGACTGTAGAACTCTGAACCCCTATA18550.4861149327693876Illumina DpnII expression Adapter 1 (95% over 23bp)
TGCACGTCAAAAAGGGCAGATGTTGCGGTAAAAGACGATCGCA17930.46986742558248623No Hit
TGCACTTTAGACCTACAGTCCGACGATCCATAATGCACGTATT17740.4648883507994035No Hit
TGCACTCAGCCAGGACGCCGGCTCCCAGCGCGACTTCCGCACT14790.38758166337785677No Hit
TGCACGTACGCGTTGAAAACATGTTTCTGCAAATTGCAGGAAG14170.3713341561909554No Hit
TGCTGGTATAACTAACGCCAGCCGGTCCATAGCGGTTTCCCGT13470.3529901964638087No Hit
TGCACGTAAAAGACCGGCCGACGATCAAAAATACGTGCATCTT12650.331501557926294No Hit
TGCACGGAGTTATTGTGGATGTTGAGCGTCACTGGCGAATACA12250.3210192952250673No Hit
TGCACGTATACTTGTTTTAGGAAAGTATGGCGGAATGGGAAGG12070.3163022770095153No Hit
TGCACATATCTCTGTTTCGGACGTCTGGTTCACAGATCTTGGT11940.3128955416316166No Hit
TGCACGTAAAAGAAGTCGGACTGTAGAACTCTGAACCCCTATA11470.30057888295767526Illumina DpnII expression Adapter 1 (95% over 23bp)
TGCACGTATGTACGTGCCACTGTATCATAAGCACTTAGTATAG11370.29795831728236855No Hit
TGAACTTATTCATCAAGACTCCAGCCCTGAGATGCTCCCACCC11330.29691009101224586No Hit
GGCACGCATTGCCCCTTCATCGCTTCGGTTTCGCGGTTCGGCG11110.29114484652657124No Hit
TGCACGTATGGTCCAGAAACAGGGTGTGTGTGTGTGTGTGTGT10920.28616577174348856No Hit
TGCACGTAAGCGAGAAGAGGAAGAATGCCAAGAAAGGCCAGGG10860.2845934323383046No Hit
GGCACGCATTGCCTCCTTCATCGCTTCGGTTTCGCGGTTCGGC9380.2458090603437658No Hit
TGCACATGCGTCCTCGCTGCTGCTGCGCGAGGGGCGCGGTGTG8460.22169985613094442No Hit
TGCACATGCGTCCCGCTGCTGCTGCGCGAGGGGCGCGGTGTGC7580.19863887818824572No Hit
TGCACGTACCTTTCCACACTCCCTTTGCTGCTCAGGTCTCTAA7550.1978527084856537No Hit
TGCACGTATCGGGTGAGTGTTGGGTTGACTGCTGCTCATTATG7300.19130129429738704No Hit
TGCACGTAGGTTATACAGTCCGACGATCTGGACTGCAGAACTC7100.1860601629467737No Hit
TGCACGTAGTGATAGTGTCTGTTCCTGAGTAGGGGCAGGGGAA6870.18003286189356835No Hit
TGCTCCTAAGTCCTTAAACTAGTAGTTAATCAAAGAATACATG6830.1789846356234457No Hit
GGCACGCATTGCCGCCTTCATCGCTTCGGTTTCGCGGTTCGGC6800.17819846592085367No Hit
TGCACGTAACGTCCTTGCTGCCCGAGGGCTGAGGGATCCATGG6510.17059882546246433No Hit
TGCTCGTACCACTGCCGTGCATCATCCTCGACTACATTCCGGT6330.16588180724691232No Hit
GGCACGCATTGCCCCCTTCATCGCTTCGGTTTCGCGGTTCGGC5920.15513748797815496No Hit
TGCACGTCAAAAAGGCAGATGTTGCGGTAAAAGACGATCGCAT5880.15408926170803228No Hit
TGCACGTTCGTCTGGCGCAGATGGGGCAGGCAGGCCTCGAACA5530.14491728184445896No Hit
TGCACGTAGCTGTCAGTCCGACGATCTGGTATGCACGTAAGAT5470.14334494243927495No Hit
TGCACGTGGCAACCAAGGGGACCGCACAGACACCATGGCCCCA5210.1365314716834776No Hit
TGCACGTAAAAAGCTACAGTCCGACGATCTGGACTGCAGAACT5190.13600735854841625No Hit
TGCACCTGGTTGCGGCCCCCGCCCGCATCGATCACGCCGGTCA5050.1323385666029869No Hit
TGCACGTAGCCCACAGTCCGACGATCTCACGTGCACCCGAGAT5000.1310282837653336No Hit
TGCACGTAGAACTATAGCCCAGGCCTTGGAATCTCCTGTTGCC4940.1294559443601496No Hit
TGCACGTAAAAGAAGTCGTATTACCGGCCGACGATCAAAAATA4940.1294559443601496No Hit
TGCACGTAGCTGGACAGTCCGACGATCCAAAATGCACGTATTT4860.12735949181990425No Hit
TGCACGTAAAAGAGAGTCGTATTACCGGCCGACGATCAAAAAT4850.12709743525237358No Hit
CGCACGAAGTACGATCGTACACTCCGACGGTCAACTGCTAGTT4820.1263112655497816No Hit
TGCACATGCGTCCCCGCTGCTGCTGCGCGAGGGGCGCGGTGTG4670.12238041703682156No Hit
TGCACGTAAGGGGTCTTTGTCATTTCATGGTGACAAAGATAAT4630.12133219076669889No Hit
TGCACGTATTGATGTCCGACGATCACTAGTGCACGTCGGACTG4560.11949779479398423No Hit
TGCACGTATGACCATCTCCGTTGCACGTATTTTTTTTTTTTTT4550.11923573822645356No Hit
TGCACGGCATTAACGCCACCCCAGCGCTTCATCGACGAGCAGC4360.11425666344337089No Hit
TGCACGTAATTTTTACAGTCCGACGATCGTTGCCTAGATCGTC4360.11425666344337089No Hit
TGCACGTACGCGTATGAAAACATGTTTCTGCAAATTGCAGGAA4320.11320843717324823No Hit
TGCACGTACTCCGATTACATTAAATGTAAATGGTCTAAATATA4240.11111198463300288No Hit
TGCAGGAATCGATCGAAAAGAAAGCCAGTGACTACGTTTTTTT4190.10980170179534954No Hit
TGCACGTAAAAGACGGCCGACGATCAAAAATACGTGCATCTTT4140.10849141895769622No Hit
TGCACGTCATCCAGCGACTCACTATAGTGAGTCGTATTACCGG3960.10377440074214421No Hit
TGCACCTGGTTGCGCCCCCGCCCGCATCGATCACGCCGGTCAC3920.10272617447202152No Hit
TGCACGTAACGTCGCTTGCTGCCCGAGGGCTGAGGGATCCATG3920.10272617447202152No Hit
TGCACGTAATTGTGACTGGTGACATATAAAGAATCAACACCAA3860.10115383506683752No Hit
TGCACGGCATTAAGCGCCACCCCAGCGCTTCATCGACGAGCAG3860.10115383506683752No Hit
TGCACGTAGTAGGACAGTCCGACGATCGGACGTGCACACGAGA3850.10089177849930686No Hit
TGCACGTAGTTAGACAGTCCGACGATCCAGAGTGCAATAAGGA3840.10062972193177619No Hit
TGCACGTCATAAATACGACTCACTATAGGGAGTCGTATTACCG3830.10036766536424553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAC251.2304097E-437.00852613
GCGACTC308.284316E-637.00852214
TCCCTTA200.001838313937.00852213
AGCGACT308.284316E-637.00852213
TGGGGCG452.6047928E-937.00852213
CTATCTA251.2313598E-437.00367416
CCCCGCT551.2732926E-1137.00367412
CGCTACA501.7826096E-1037.00367412
TCGGTGT452.6084308E-937.00367412
GATACCG308.291772E-637.0036712
GATATCT200.001839498237.0036715
GATGAGT355.6137833E-737.0036712
CGCCATT200.001839498237.0036712
AACGTCT308.299234E-636.998828
AGCCGTT251.2323103E-436.998828
TTGGCGG308.299234E-636.9988217
GTACCGT600.036.998826
TACCCGT600.036.998827
GTATTGG551.2732926E-1136.998826
CGTATGG4800.036.998825
Produced by FastQC (version 0.11.5)