FastQCFastQC Report
Thu 26 May 2016
SRR2099081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187215
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTCATAAAACGACTCACTATAGGGAGTCGTATTACCGG38182.0393665037523703No Hit
TGCACGTCATCCACGACTCACTATAGTGAGTCGTATTACCGGC36521.9506983948935717No Hit
TGCACGTCATAAAACGACTCACTATAGTGAGTCGTATTACCGG29881.5960259594583768No Hit
TGCACGTAAAAGAGTCGGACTGTAGAACTCTGAACCCCTATAG21481.1473439628234918Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGCACGTATAACGCAGTCCGACGATCTACATTGCACGTATTTT14150.7558155062361456No Hit
TGCACGTCAAAAAGGGCAGATGTTGCGGTAAAAGACGATCGCA8980.4796624202120557No Hit
TGCACTTTAGACCTACAGTCCGACGATCCATAATGCACGTATT8430.4502844323371525No Hit
TGCACGTAAAAGAGGTCGGACTGTAGAACTCTGAACCCCTATA8280.4422722538258152Illumina DpnII expression Adapter 1 (95% over 23bp)
TGCACTCAGCCAGGACGCCGGCTCCCAGCGCGACTTCCGCACT7110.3797772614373848No Hit
TGCTGGTATAACTAACGCCAGCCGGTCCATAGCGGTTTCCCGT6270.33490906177389634No Hit
TGCACGTACGCGTTGAAAACATGTTTCTGCAAATTGCAGGAAG6150.32849931896482654No Hit
TGCACGTATGGTCCAGAAACAGGGTGTGTGTGTGTGTGTGTGT5700.30446278343081484No Hit
TGCACGTAAAAGACCGGCCGACGATCAAAAATACGTGCATCTT5590.2985871858558342No Hit
TGCACGTATACTTGTTTTAGGAAAGTATGGCGGAATGGGAAGG5570.2975188953876559No Hit
TGCACGGAGTTATTGTGGATGTTGAGCGTCACTGGCGAATACA5480.29271158828085353No Hit
TGCACATATCTCTGTTTCGGACGTCTGGTTCACAGATCTTGGT5400.2884384264081404No Hit
TGCACGTAAGCGAGAAGAGGAAGAATGCCAAGAAAGGCCAGGG5370.28683599070587296No Hit
GGCACGCATTGCCCCTTCATCGCTTCGGTTTCGCGGTTCGGCG5250.28042624789680315No Hit
TGCACGTAAAAGAAGTCGGACTGTAGAACTCTGAACCCCTATA4980.2660043265763961Illumina DpnII expression Adapter 1 (95% over 23bp)
TGAACTTATTCATCAAGACTCCAGCCCTGAGATGCTCCCACCC4970.26547018134230693No Hit
TGCACGTATGTACGTGCCACTGTATCATAAGCACTTAGTATAG4930.2633336004059504No Hit
GGCACGCATTGCCTCCTTCATCGCTTCGGTTTCGCGGTTCGGC4560.24357022674465187No Hit
TGCACATGCGTCCTCGCTGCTGCTGCGCGAGGGGCGCGGTGTG4210.22487514355153168No Hit
TGCACGTAGGTTATACAGTCCGACGATCTGGACTGCAGAACTC3490.18641668669711295No Hit
TGCACATGCGTCCCGCTGCTGCTGCGCGAGGGGCGCGGTGTGC3470.18534839622893465No Hit
TGCACGTATCGGGTGAGTGTTGGGTTGACTGCTGCTCATTATG3290.17573378201532996No Hit
TGCACGTACCTTTCCACACTCCCTTTGCTGCTCAGGTCTCTAA3110.16611916780172528No Hit
TGCTCGTACCACTGCCGTGCATCATCCTCGACTACATTCCGGT3080.16451673209945786No Hit
GGCACGCATTGCCGCCTTCATCGCTTCGGTTTCGCGGTTCGGC3020.16131186069492295No Hit
TGCACGTCAAAAAGGCAGATGTTGCGGTAAAAGACGATCGCAT2820.15062895601313997No Hit
TGCACGTAGCTGTCAGTCCGACGATCTGGTATGCACGTAAGAT2690.14368506796998104No Hit
TGCACGTTCGTCTGGCGCAGATGGGGCAGGCAGGCCTCGAACA2640.1410143417995353No Hit
TGCACGTGGCAACCAAGGGGACCGCACAGACACCATGGCCCCA2620.13994605133135699No Hit
TGCACGTAACGTCCTTGCTGCCCGAGGGCTGAGGGATCCATGG2600.1388777608631787No Hit
GGCACGCATTGCCCCCTTCATCGCTTCGGTTTCGCGGTTCGGC2550.13620703469273296No Hit
TGCACGTAAAAAGCTACAGTCCGACGATCTGGACTGCAGAACT2460.13139972758593063No Hit
TCCAAGTATCCCAGTCTACGGCCATACCACCCTGAACGCGCCC2380.12712656571321743No Hit
TGCACGTAGCTGGACAGTCCGACGATCCAAAATGCACGTATTT2380.12712656571321743No Hit
TGCACGTAGAACTATAGCCCAGGCCTTGGAATCTCCTGTTGCC2360.12605827524503913No Hit
TGCACGTAATTTTTACAGTCCGACGATCGTTGCCTAGATCGTC2320.12392169430868252No Hit
TGCACCTGGTTGCGGCCCCCGCCCGCATCGATCACGCCGGTCA2290.12231925860641508No Hit
TGCACGTAAAAGAGAGTCGTATTACCGGCCGACGATCAAAAAT2280.12178511337232593No Hit
TGCACGTAGCCCACAGTCCGACGATCTCACGTGCACCCGAGAT2270.1212509681382368No Hit
CGCACGAAGTACGATCGTACACTCCGACGGTCAACTGCTAGTT2250.1201826776700585No Hit
TGCACGTACTTACGGAAGGGTGGGTGGTCACCCTGCACCCTGC2240.11964853243596933No Hit
TGCACGTATTGATGTCCGACGATCACTAGTGCACGTCGGACTG2210.1180460967337019No Hit
TGCTCCTAAGTCCTTAAACTAGTAGTTAATCAAAGAATACATG2130.11377293486098872No Hit
TGCACGTACTCCGATTACATTAAATGTAAATGGTCTAAATATA2100.11217049915872125No Hit
TGCACGTAAGGGGTCTTTGTCATTTCATGGTGACAAAGATAAT2090.1116363539246321No Hit
TGCACGTAAAAGAAGTCGTATTACCGGCCGACGATCAAAAATA2060.11003391822236466No Hit
TGCACGTATTCGTGGAGCAGAAGTGAGGGATGCTGAGGGCCGG2020.10789733728600807No Hit
TGCACATGCGTCCCCGCTGCTGCTGCGCGAGGGGCGCGGTGTG1970.10522661111556232No Hit
TGCACGGCATTAACGCCACCCCAGCGCTTCATCGACGAGCAGC1960.10469246588147318No Hit
TGCACGTAGTAGGACAGTCCGACGATCGGACGTGCACACGAGA1940.10362417541329488No Hit
TGCACGGCATTAAGCGCCACCCCAGCGCTTCATCGACGAGCAG1910.10202173971102743No Hit
TGCACGTAGTGATAGTGTCTGTTCCTGAGTAGGGGCAGGGGAA1900.1014875944769383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCC308.216353E-637.03824613
TAGAATG200.001828696237.03824613
CGGTTCA251.2222218E-437.03824613
CGCCTTC600.037.03824613
CGGGAAA200.001828696237.03824613
GCGATCT200.001828696237.03824613
CGGAGAG308.216353E-637.03824613
TTGCCCC850.037.0184489
TCACGGA251.2260766E-437.01844410
TTGGCGA200.001833506937.01844412
TGCCCCT600.037.01844410
TGCCCCC355.5759483E-737.01844410
CCCTCGT501.7644197E-1037.01844410
CTCCGGT251.2260766E-437.0184449
GTCTGGC551.2732926E-1137.01844410
CCGATTA403.787318E-837.01844411
CTTCCCG200.001833506937.0184449
ACTTGTT251.2260766E-437.01844410
TCCGATT551.2732926E-1137.01844410
ACCGCGA200.001833506937.0184449
Produced by FastQC (version 0.11.5)