##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099080_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 370399 Sequences flagged as poor quality 0 Sequence length 43 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.649604885542345 37.0 35.0 37.0 30.0 37.0 2 34.711197924400444 37.0 35.0 37.0 31.0 37.0 3 32.95025634518452 35.0 32.0 37.0 26.0 37.0 4 35.395190051808996 37.0 35.0 39.0 29.0 39.0 5 33.19280289633611 35.0 32.0 39.0 24.0 39.0 6 35.03496229741441 37.0 34.0 39.0 28.0 39.0 7 35.85926797858526 39.0 35.0 39.0 30.0 39.0 8 35.1485506170373 39.0 35.0 39.0 27.0 39.0 9 30.705771343875117 31.0 30.0 34.0 27.0 34.0 10 30.95763217503287 31.0 31.0 34.0 27.0 34.0 11 30.85151687774535 31.0 30.0 34.0 27.0 34.0 12 34.24011403918477 37.0 35.0 37.0 30.0 37.0 13 34.11689016439029 37.0 35.0 37.0 28.0 37.0 14 31.87326639650755 31.0 31.0 34.0 30.0 34.0 15 31.997872564450766 31.0 31.0 34.0 30.0 34.0 16 32.101055888379825 33.0 31.0 34.0 30.0 34.0 17 35.68149212065907 37.0 35.0 37.0 33.0 37.0 18 35.61943741748763 37.0 35.0 37.0 33.0 37.0 19 35.60396761330349 37.0 35.0 37.0 33.0 37.0 20 35.528770326053795 37.0 35.0 37.0 33.0 37.0 21 35.61553621905027 37.0 35.0 37.0 33.0 37.0 22 37.37016838598376 39.0 37.0 39.0 35.0 39.0 23 37.4564780142495 39.0 37.0 39.0 35.0 39.0 24 37.56542269282584 39.0 37.0 39.0 35.0 39.0 25 37.65560112203327 39.0 37.0 39.0 35.0 39.0 26 37.74896260519062 39.0 37.0 39.0 35.0 39.0 27 39.13593449226375 40.0 38.0 41.0 36.0 41.0 28 39.19914470611422 40.0 39.0 41.0 36.0 41.0 29 39.24234946638625 40.0 39.0 41.0 36.0 41.0 30 39.27312438748485 40.0 39.0 41.0 36.0 41.0 31 39.33044095691403 40.0 39.0 41.0 36.0 41.0 32 39.390848787388734 40.0 39.0 41.0 37.0 41.0 33 39.40770088472161 40.0 39.0 41.0 37.0 41.0 34 39.41445036298694 40.0 39.0 41.0 37.0 41.0 35 39.40205562110049 40.0 39.0 41.0 37.0 41.0 36 39.447881878730776 41.0 39.0 41.0 37.0 41.0 37 39.471534750363794 41.0 39.0 41.0 37.0 41.0 38 39.42830569197001 41.0 39.0 41.0 37.0 41.0 39 39.40644818155557 41.0 39.0 41.0 36.0 41.0 40 39.38172889235662 41.0 39.0 41.0 36.0 41.0 41 39.36712302139045 41.0 39.0 41.0 36.0 41.0 42 39.223650711799976 41.0 39.0 41.0 36.0 41.0 43 39.20093466775018 41.0 39.0 41.0 36.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.25623907750188124 1101 2 -0.07068763771048481 1101 3 -0.11031989870926395 1101 4 0.014343457014412309 1101 5 -0.5538682661357441 1101 6 -0.15452035948926834 1101 7 0.03322834366508687 1101 8 0.0588374170393422 1101 9 -0.04187618214899658 1101 10 0.02615376689755422 1101 11 -0.017695108749382626 1101 12 0.025016944474792524 1101 13 -0.20954437089957878 1101 14 0.1278838380814129 1101 15 0.20982250625736043 1101 16 0.13214380554590832 1101 17 0.24708848828345253 1101 18 0.13818013703991028 1101 19 0.2865595834670458 1101 20 0.1908579554328611 1101 21 0.24414702303639757 1101 22 0.23605026452807465 1101 23 0.1888217584349121 1101 24 0.23341225175081348 1101 25 0.24647517012823528 1101 26 0.11399509074611558 1101 27 0.16983214036913807 1101 28 0.32420729713538066 1101 29 0.2894959162950954 1101 30 0.1180111653420397 1101 31 0.12988551669573667 1101 32 0.15877489478980777 1101 33 0.11941488258766952 1101 34 0.13634866394489364 1101 35 0.19930233532875263 1101 36 0.10400341745415176 1101 37 0.2168447591253866 1101 38 0.2637131259634202 1101 39 0.16113434536963211 1101 40 0.18426783583649353 1101 41 0.2450316712803442 1101 42 0.25936594087799136 1101 43 0.39264387048702787 1102 1 0.25530813916363115 1102 2 0.07539775926819914 1102 3 -0.26919069956461783 1102 4 -0.026092495251575087 1102 5 -0.26220447362694443 1102 6 -0.04577027326453731 1102 7 0.11987040754037537 1102 8 0.12102959473127584 1102 9 -0.0037786447274612556 1102 10 -0.06286120171664322 1102 11 0.15165372204319638 1102 12 0.1050197036663505 1102 13 -0.07725493170526221 1102 14 0.11669531631829244 1102 15 0.17499461083071566 1102 16 0.1608187038007216 1102 17 0.17714298231670256 1102 18 0.06484082545819803 1102 19 0.05228352635177913 1102 20 0.16804633923640466 1102 21 0.13535899792775297 1102 22 0.012348809054529397 1102 23 0.06106774036220486 1102 24 -0.0024710344463301226 1102 25 0.0024867587327790375 1102 26 0.07824286614792442 1102 27 0.1768887727758468 1102 28 0.29294565679600026 1102 29 0.04198953566461938 1102 30 0.2645587269065075 1102 31 0.1882217241869384 1102 32 0.195178978393308 1102 33 0.15402894066865258 1102 34 0.17624312486784532 1102 35 0.27289342314002596 1102 36 0.24852987120320336 1102 37 0.2538696608291886 1102 38 0.3020348476989483 1102 39 0.277599820985273 1102 40 0.026935284274102855 1102 41 0.16588081248197284 1102 42 0.34003435503264257 1102 43 0.3162246113299574 1103 1 0.2919114633841531 1103 2 0.24955538300400804 1103 3 0.0039018989292500805 1103 4 0.4053483916544849 1103 5 0.24030294800564178 1103 6 0.1312385991504712 1103 7 0.46148018900054666 1103 8 0.5419510512161878 1103 9 0.38470901942898905 1103 10 0.25907496385704576 1103 11 0.15505509399697104 1103 12 0.32104036683324466 1103 13 0.5712833840453655 1103 14 0.19349118368491602 1103 15 0.358926985920867 1103 16 0.26397017219379393 1103 17 0.32024212191048207 1103 18 0.12263395898050078 1103 19 0.28385858966627353 1103 20 0.3190417604664688 1103 21 0.2934590599843432 1103 22 0.3052726807147934 1103 23 0.3570977958776993 1103 24 0.32266188405827023 1103 25 0.21573332465532502 1103 26 0.28538105107656264 1103 27 0.43529240895651355 1103 28 0.3020495261081777 1103 29 0.4577753901167583 1103 30 0.511208509708446 1103 31 0.42209455849585 1103 32 0.44531734781450893 1103 33 0.3383671993943125 1103 34 0.29837049182354747 1103 35 0.3709528968330673 1103 36 0.22220110545844562 1103 37 0.3335201312852192 1103 38 0.3139564215882231 1103 39 0.48712299157380556 1103 40 0.3160082930123451 1103 41 0.30647756205847543 1103 42 0.41482428509318936 1103 43 0.44049544641038096 1104 1 0.2751983081469618 1104 2 0.074292930940274 1104 3 -0.270194496393529 1104 4 0.011178350642794044 1104 5 -0.23406231253058962 1104 6 -0.10542448481145783 1104 7 0.002188265672593559 1104 8 -0.1450515237851917 1104 9 -0.2657239899455739 1104 10 -0.10923465976412672 1104 11 -0.1276625228441226 1104 12 -0.1303804299559559 1104 13 0.217157374583131 1104 14 0.054645680520550854 1104 15 0.09950452658348752 1104 16 0.10187497182751315 1104 17 0.16022835393336976 1104 18 0.13680458448178 1104 19 -9.242237419186949E-4 1104 20 0.01591571528542346 1104 21 0.011605188877283013 1104 22 0.1350206033413599 1104 23 -0.04131953618708195 1104 24 0.005751625941172733 1104 25 0.06609749414431576 1104 26 0.18080287396971784 1104 27 -0.12129754886282029 1104 28 0.13631750159857603 1104 29 0.10222712264133094 1104 30 0.14245727172701095 1104 31 0.08761064657193884 1104 32 0.2510070293001476 1104 33 0.2650364854402696 1104 34 0.16067123769721547 1104 35 0.21738150305268533 1104 36 0.23677762651143297 1104 37 0.21500512451488163 1104 38 0.2943792673381722 1104 39 0.17188502312974663 1104 40 0.11060566086086965 1104 41 0.2798378252086806 1104 42 0.42563084826190334 1104 43 0.19150937349855468 1105 1 0.22117842458667525 1105 2 0.20229951767798582 1105 3 0.06920443295322087 1105 4 0.10493451800020637 1105 5 -0.17538299044652206 1105 6 0.05886466557338821 1105 7 0.005006748009286355 1105 8 0.1597146100217941 1105 9 -0.06490249793846914 1105 10 -0.12616713953519465 1105 11 -0.013557159710117617 1105 12 0.09639330203535934 1105 13 0.2198494260928996 1105 14 0.03703170775560238 1105 15 0.18883253132001698 1105 16 0.11059253152753001 1105 17 0.2157993278824506 1105 18 -0.06102882285983924 1105 19 0.0963966761988786 1105 20 0.11459960121398183 1105 21 0.018087081515929526 1105 22 0.1877915970259849 1105 23 0.002718479404002494 1105 24 0.03422939878506526 1105 25 -0.06520246145070985 1105 26 0.0031058885740193887 1105 27 0.03083882374424718 1105 28 0.010284469232288984 1105 29 0.22469850610294628 1105 30 0.016677101014558104 1105 31 0.1316085743398432 1105 32 0.05420357064611636 1105 33 0.12436214658098521 1105 34 0.17530020947538816 1105 35 0.09215947818589854 1105 36 -0.10342922665611809 1105 37 0.03132888610951312 1105 38 0.17890475085899027 1105 39 0.043992532504134374 1105 40 0.20819484084902484 1105 41 -0.04583873389927362 1105 42 0.22910800437005463 1105 43 0.21575633915870895 1106 1 0.4673057813503547 1106 2 0.3112969778646999 1106 3 -0.01905693900357619 1106 4 0.4631053948078261 1106 5 -0.43845101625651495 1106 6 -0.07124070235632018 1106 7 0.00401713607445231 1106 8 0.2125701838823133 1106 9 0.3134953603811361 1106 10 0.08387942370844215 1106 11 0.06380704036310192 1106 12 0.13148162347047077 1106 13 0.29056091703194653 1106 14 0.09565334421908389 1106 15 0.2292636131431891 1106 16 0.09844547404995296 1106 17 0.24391746352220878 1106 18 0.23452478779364583 1106 19 0.18048508915878614 1106 20 0.11274050364853849 1106 21 0.13684623399625195 1106 22 -0.02700853568497763 1106 23 -0.008484728540352648 1106 24 0.017757794355262035 1106 25 0.26940935897762586 1106 26 0.25195324808344566 1106 27 0.27038211497617226 1106 28 0.36934485272121265 1106 29 0.4068671863152815 1106 30 0.26198931499954625 1106 31 0.14667584507021303 1106 32 0.2389950085007726 1106 33 0.2608227680344015 1106 34 0.14150467588578408 1106 35 0.20830445347419868 1106 36 0.04019893094959315 1106 37 0.06380875065280378 1106 38 0.2808719620982387 1106 39 0.11266473642139374 1106 40 0.1518298628149779 1106 41 0.27642295839434183 1106 42 0.402987969590626 1106 43 0.0678894391037943 1107 1 0.43190172905997315 1107 2 0.5403000588035312 1107 3 0.6278038196776095 1107 4 0.37558747615316435 1107 5 0.35052339483486605 1107 6 0.6226498387335582 1107 7 0.5274183641708987 1107 8 0.6667492420564258 1107 9 0.2784416579056881 1107 10 0.21719956320650624 1107 11 0.38712375659579834 1107 12 0.5032295213715798 1107 13 0.3823900105084874 1107 14 0.13822218394608043 1107 15 0.2110186785061643 1107 16 0.13086219242219244 1107 17 0.21410831056643786 1107 18 0.06444466198864518 1107 19 0.23708931689152024 1107 20 0.13087846124283686 1107 21 0.2409857106645532 1107 22 0.20819653930593063 1107 23 0.2135996121601309 1107 24 0.1405380207186866 1107 25 0.14685110997785955 1107 26 0.17209488568801845 1107 27 0.2658676837731022 1107 28 0.15785391367673185 1107 29 0.2821480010524411 1107 30 0.1940535440160005 1107 31 0.240104245335516 1107 32 0.2703606774282221 1107 33 0.28645179304563584 1107 34 0.24041564959082962 1107 35 0.2593672703936889 1107 36 0.31059945299755753 1107 37 0.27853307081369394 1107 38 0.2638840077132514 1107 39 0.22504411980572314 1107 40 0.3286629144420985 1107 41 0.3171437697082311 1107 42 0.3583919149039616 1107 43 0.3676096941370588 1108 1 0.3098631921691819 1108 2 0.3039308681186341 1108 3 -0.017711823310818886 1108 4 0.37345663654176775 1108 5 0.23811655595057601 1108 6 0.20985773192901291 1108 7 0.19322495260579586 1108 8 0.4304507427330435 1108 9 0.3331340494954347 1108 10 0.19507821643508194 1108 11 0.1845728083216791 1108 12 0.27298523222371074 1108 13 0.2676478320026945 1108 14 0.14369319038909012 1108 15 0.056704485700521445 1108 16 0.04537361412646135 1108 17 -0.05071667781998457 1108 18 -0.09530014219628669 1108 19 -0.05983712369744154 1108 20 -0.07436380815183696 1108 21 -0.07684012601820456 1108 22 -0.11791785951453448 1108 23 0.035396788545178026 1108 24 -0.07472979855379691 1108 25 -0.03694138057979046 1108 26 -0.0478461375189454 1108 27 0.10533770053705638 1108 28 0.028577086305034527 1108 29 0.04091372988037989 1108 30 0.0032121148386465848 1108 31 -0.03185047179805167 1108 32 -0.1104839833542286 1108 33 -0.14485387640156233 1108 34 -0.14509464105165648 1108 35 -0.029924503151583792 1108 36 0.025061895687059632 1108 37 0.08415202417759815 1108 38 0.1582307185451839 1108 39 0.011424924071441467 1108 40 0.079849519438973 1108 41 0.1245350564929879 1108 42 0.33007245054250944 1108 43 0.2521984723883577 1109 1 0.10766346732171428 1109 2 0.12257195357542372 1109 3 0.2822119115856978 1109 4 -0.0670740290083387 1109 5 0.06460098391245594 1109 6 0.2267332553169723 1109 7 0.3129661497186831 1109 8 0.2783043536115386 1109 9 0.09695647642050531 1109 10 0.06680828781523118 1109 11 0.2534033103753508 1109 12 0.1145556951977511 1109 13 -0.006512753394282811 1109 14 -0.19053239480849982 1109 15 -0.12206490457742092 1109 16 -0.09360084454084472 1109 17 -0.2028705439124181 1109 18 -0.20085254080856174 1109 19 0.040306932609134094 1109 20 -0.0699623645979841 1109 21 0.08566186784071306 1109 22 -0.03322870211930962 1109 23 -0.08048130692078814 1109 24 -0.10425884907818528 1109 25 -0.014321051194301049 1109 26 -0.048309709716576776 1109 27 -0.011896218990798957 1109 28 0.04567442649724285 1109 29 -0.08513055372611689 1109 30 0.008530733493188336 1109 31 0.02583934357061679 1109 32 0.018279425346968026 1109 33 -0.13939319529935545 1109 34 -0.030896371721190974 1109 35 -0.11457129104486796 1109 36 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0.4351800029112738 2216 32 0.3014753960429388 2216 33 0.24613794023178315 2216 34 0.23283989201507183 2216 35 0.25882614485256283 2216 36 0.1964219638200717 2216 37 0.1305352353158611 2216 38 0.2530614968907372 2216 39 0.23694888171048234 2216 40 0.10030793608711974 2216 41 0.3214727740372325 2216 42 0.43793736944942196 2216 43 0.5632265772539427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 3.0 25 23.0 26 146.0 27 713.0 28 1797.0 29 3243.0 30 5275.0 31 8089.0 32 12715.0 33 18047.0 34 24677.0 35 34112.0 36 55745.0 37 103158.0 38 102656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.070234836089917 2.8138212567429655 0.8780029482755828 95.23794095889153 2 1.5380835646173283 2.8258944481041914 94.42380587263361 1.2122161146448664 3 1.8050902140136125 95.04477628058542 1.0094574200115012 2.1406760853894604 4 96.02428732258997 0.9489766441054106 0.9549161849789011 2.0718198483257244 5 1.5375394509134794 95.70784860568526 1.3266846113764421 1.4279273320248274 6 1.6987270346521957 0.8866210397008599 96.77020478138203 0.6444471442649063 7 2.057780933533838 2.5081061233966615 2.6657739356747725 92.76833900739473 8 91.92450160910603 4.770891379943412 1.323462709777749 1.9811443011728116 9 20.087390525397026 21.517147080361994 43.50475459583908 14.890707798401895 10 23.659993411352996 28.08248770029217 30.760880718486554 17.49663816986828 11 26.51704387416995 18.50940700874135 27.35391254330843 27.619636573780276 12 40.12773944394183 11.930810051716922 16.78220829901292 31.15924220532833 13 29.236773165527858 21.084884003564966 35.54919383152834 14.129148999378833 14 29.27251467041855 26.543398244140608 18.158373008050162 26.025714077390678 15 28.83702595726528 20.828720928348876 23.071294731328535 27.262958383057306 16 33.148213644500004 20.176786153011278 27.561414829194014 19.113585373294708 17 29.973244923689986 23.062281821937002 24.390316336255424 22.574156918117588 18 22.150227593021558 30.796062613729013 18.357280546871205 28.69642924637822 19 32.285363252190585 20.051574925405376 20.09099869037493 27.572063132029108 20 24.60561094866665 21.455358372279505 32.01334834482144 21.925682334232402 21 22.811616662032026 27.77842272792313 22.24331059209123 27.166650017953614 22 22.859132989019948 20.759505290241066 28.55839243626468 27.822969284474308 23 20.744656438057337 24.521394496205442 31.329188253747986 23.404760811989235 24 22.338073266936465 35.341078134660194 23.690398732178004 18.63044986622534 25 18.723862645417512 23.94309919843196 33.69474539618088 23.638292759969655 26 18.995793532083095 22.90392081688635 30.310598247215037 27.78968740381552 27 23.935539782774793 29.77248858663225 22.55702634186377 23.734945288729183 28 25.86670050405104 24.331059209123136 22.698765385435706 27.103474901390122 29 23.645852175626825 24.270583883865775 22.895850150783346 29.187713789724057 30 28.587820161501515 23.801360154860028 19.93228923404221 27.678530449596245 31 34.075237987246155 24.825188040972144 20.470412907197122 20.62916106458458 32 22.480115768613963 31.724056005226863 19.659605717155245 26.136222509003925 33 21.81674356572237 29.899918736281684 22.83888455422396 25.444453143771987 34 29.113548129309553 29.290924897002686 23.082198068024127 18.513328905663638 35 25.814733920631106 34.20303080208533 21.46858640863722 18.513648868646342 36 23.285969994519423 26.270319304317773 27.13452250141064 23.30918819975216 37 25.09024247349423 25.2981308941092 24.00733279156351 25.604293840833066 38 22.9358070621141 27.260332776276393 26.48306285924098 23.320797302368526 39 29.154506356658633 24.53813320230346 28.008714926336193 18.298645514701715 40 28.08444232051687 23.983012179284685 25.040836107488733 22.891709392709707 41 21.23336861203508 27.591634627845696 27.615933733638602 23.559063026480626 42 26.32088205161563 28.150358577399054 20.411266322996113 25.117493047989203 43 36.86624747227945 25.49076251441037 21.258231122078442 16.384758891231748 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.5 7 5.0 8 20.5 9 36.0 10 63.5 11 91.0 12 91.0 13 167.0 14 243.0 15 243.5 16 244.0 17 322.5 18 401.0 19 401.0 20 593.0 21 785.0 22 1010.5 23 1236.0 24 1362.0 25 1488.0 26 1488.0 27 1983.5 28 2479.0 29 3397.5 30 4316.0 31 5753.0 32 7190.0 33 7190.0 34 8302.0 35 9414.0 36 10495.5 37 11577.0 38 14277.0 39 16977.0 40 16977.0 41 16507.0 42 16037.0 43 22241.5 44 28446.0 45 28506.5 46 28567.0 47 28567.0 48 32595.0 49 36623.0 50 46143.5 51 55664.0 52 50999.5 53 46335.0 54 46335.0 55 38242.5 56 30150.0 57 31734.5 58 33319.0 59 29062.0 60 24805.0 61 24805.0 62 15757.0 63 6709.0 64 5393.5 65 4078.0 66 2629.5 67 1181.0 68 1181.0 69 1215.0 70 1249.0 71 961.5 72 674.0 73 360.0 74 46.0 75 46.0 76 28.0 77 10.0 78 16.0 79 22.0 80 11.0 81 0.0 82 0.0 83 1.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0035097286979716468 2 8.099373918396108E-4 3 5.399582612264071E-4 4 0.0 5 5.399582612264071E-4 6 0.0010799165224528143 7 0.0 8 0.0018898539142924251 9 0.0907129878860364 10 0.017548643489858236 11 0.024298121755188324 12 0.030777620889905207 13 0.03482730784910326 14 0.025917996538867547 15 0.005669561742877275 16 0.0021598330449056286 17 5.399582612264071E-4 18 2.699791306132036E-4 19 0.01727866435924503 20 0.004319666089811257 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0045896452204244615 27 0.0 28 0.0 29 0.0 30 0.0 31 2.699791306132036E-4 32 0.001349895653066018 33 0.0 34 2.699791306132036E-4 35 5.399582612264071E-4 36 0.0 37 0.0021598330449056286 38 0.0 39 0.0 40 0.004319666089811257 41 0.004049686959198055 42 0.38903992721362635 43 0.0016198747836792217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 370399.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.576699721111558 #Duplication Level Percentage of deduplicated Percentage of total 1 67.48139377537213 6.4624904494882545 2 9.359494812810103 1.7926614272716717 3 4.166666666666666 1.1970874651389447 4 2.263757329724853 0.86717296752961 5 1.5279657194406855 0.7316434439617818 6 1.0599909788001805 0.6090729186633873 7 0.8260036084799279 0.5537271968876806 8 0.6822282363554353 0.5226795968671621 9 0.5271763644564726 0.4543748768220217 >10 8.852052322958953 22.656648641060045 >50 2.0354082092918357 12.860725865890565 >100 1.0374379792512405 20.259773919476025 >500 0.09021199819576003 5.745965836840812 >1k 0.07329724853405503 11.576165162432945 >5k 0.011276499774470004 6.737599183583109 >10k+ 0.005638249887235002 6.972211048085983 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAGATCGGCGCGAGGGAGTCGTATTACCGGCCGACGATCTAG 13977 3.7734983085807463 No Hit CTAGATCGTACGTGAGTCGTATTACCGGCCGACGATCTAGACG 11848 3.198712739505236 No Hit CTAGATCGTACGTGTGAGTCGTATTACCGGCCGACGATCTAGA 7116 1.9211714934435569 No Hit CTAGATCGTTGGTCCGGCCGACGATCTAGACCAAGATCGTCGG 6307 1.7027583767774752 No Hit CTAGATCGAGTCGCCGACGATCTAGCGACTGATCGTCGGACTG 5869 1.5845075175688919 No Hit CTAGATCGTGGTCACTCACTATAGTGAGTCGTATTACCGGCCG 5664 1.5291617957931853 No Hit CTAGATCGTACGTACCGGCCGACGATCTAGACGTAGATCGTCG 4571 1.2340746060329537 No Hit CTAGATCGTTAGAAGTGAGTCGTATTACCGGCCGACGATCTAG 3651 0.9856938058688064 No Hit CTAGATTGCTTGACTTTACTGCTTGTGCAGAACAGCAGTGGAA 2701 0.7292136317862629 No Hit CTAGATCGTACGTCCGGCCGACGATCTAGACGTAGATCGTCGG 2094 0.5653362995040484 No Hit CTAGATCGAGTCGGCCGACGATCTAGCGACTGATCGTCGGACT 2081 0.5618265708060767 No Hit CTAGATCGAGTCGTCCGACGATCTAGCGACTGATCGTCGGACT 1915 0.5170100351242849 No Hit CTAGATCGTACGTCGGCCGACGATCTAGACGTAGATCGTCGGA 1841 0.4970315794589078 No Hit CTAGATCGGCGCGATTACCGGCCGACGATCTAGCGCGCGATCG 1716 0.46328418813225736 No Hit CTAGATCGTGGCACTCACTATAGTGAGTCGTATTACCGGCCGA 1646 0.44438564898933314 No Hit CTAGATCGTACGTTACCGGCCGACGATCTAGACGTAGATCGTC 1511 0.4079384663565506 No Hit CTACATGGTGTGGGGAGATCGTCGCCATGCCGAAGATGATCGC 1467 0.3960593846095697 No Hit CTAGATCGAGTCGGGCCGACGATCTAGCGACTGATCGTCGGAC 1421 0.3836403446013623 No Hit CTAGATCGTACGTGGTGAGTCGTATTACCGGCCGACGATCTAG 1365 0.36852151328702293 No Hit CTAGATCGTTAGACGACGATCGGCCCCTACAGTGAGTCGTATT 1357 0.36636168024211724 No Hit CTAGATCGTTAGATAAGATCGTCGGACTGTAGAACTCTGAACC 1261 0.34044368370324973 Illumina DpnII expression Sequencing Primer (100% over 27bp) CTAGATCGAGTCGCCCGACGATCTAGCGACTGATCGTCGGACT 1209 0.32640476891136316 No Hit CTAGTTCAATAGAACGCCTGCGACTTCGCAGACCTTCTTCGAA 1184 0.31965529064603304 No Hit CTAGATCGTACGTAGTGAGTCGTATTACCGGCCGACGATCTAG 1177 0.3177654367317406 No Hit CTAGATCGAGTCGCGGCCGACGATCTAGCGACTGATCGTCGGA 1160 0.3131757915113162 No Hit CTCAATCGACCGCCCGACGATCGGCCACTAGATCGTTTTTTTT 1146 0.30939608368273136 No Hit CTAGATCGTACGTATTACCGGCCGACGATCTAGACGTAGATCG 1146 0.30939608368273136 No Hit CTAGGTAGGTTTTGTTCACAGAGAGTGTAAGATTCAAGACTTC 1130 0.30507641759292004 No Hit CTAGATCGTACGTGGAGTCGTATTACCGGCCGACGATCTAGAC 1060 0.2861778784499958 No Hit CTAGATCGTTTCGGGGGGGGGCACTTAGGGAGGACAGATTGAC 1051 0.283748066274477 No Hit CTAGATCGTCGCCGGTCGGTCTCCATTCGTGAGAGCGGTTGGG 1009 0.2724089427887224 No Hit CTAGATCGTACGTTGAGTCGTATTACCGGCCGACGATCTAGAC 1008 0.27213896365810925 No Hit CTAGATCGTACGTTTACCGGCCGACGATCTAGACGTAGATCGT 976 0.2634996314784867 No Hit CTAGATCGGTAATCGCAAGACCAACAACGTAATCGCCATGGCT 914 0.24676092538046807 No Hit CTAGATCGTTGCGCAGGATGCCAGGAGTTAAAGGCTCTGTCTC 901 0.24325119668249642 No Hit CTAGATCGAGTCGAGTGAGTCGTATTACCGGCCGACGATCTAG 815 0.2200329914497609 No Hit CTAGATCGATCCTGCCAGAGTACCCCACCCGTAGTGGCCTGGT 774 0.20896384709461957 No Hit CTAGATCGAGTCGCCGGCCGACGATCTAGCGACTGATCGTCGG 744 0.2008644731762235 No Hit CTAGATCGTTAGAACCGGCCGACGATCTAGTCTAAGATCGTCG 744 0.2008644731762235 No Hit CTAGATCGTTAGACCGACGATCGGCCCCTACAGTGAGTCGTAT 726 0.19600484882518582 No Hit CTAGATCGTTAGACGGCCGACGATCTAGTCTAAGATCGTCGGA 726 0.19600484882518582 No Hit CTAGATCGTCGCCGTCGGTCTCCATTCGTGAGAGCGGTTGGGT 724 0.19546489056395938 No Hit CTAGATCGTACGTTCGTATTACCGGCCGACGATCTAGACGTAG 698 0.1884454331680161 No Hit CTAGATCGTGACTCAGTCCGACGATCCCTAGCTAGATCGTCGG 692 0.1868255583843369 No Hit CTAGATCGACCTCGTACAGTGCTCTACGCCGGAAGTTCTACCA 675 0.18223591316391244 No Hit CTACATCGAGCTTGATGAGGTCCCTAGTGGAGCAGCAGTATCA 661 0.17845620533532758 No Hit CTAGATCGTTTCGGGGGGGGGGCACTTAGGGAGGACAGATTGA 660 0.17818622620471436 No Hit CTAGATCGTACGTAGTCGTATTACCGGCCGACGATCTAGACGT 649 0.17521645576796913 No Hit CTAGATCGTTAGAAGTCGTATTACCGGCCGACGATCTAGTCTA 645 0.1741365392455163 No Hit CTAGATCGCGTAGCTACAGTCCGACGATCAGTTTCTAGATCGT 637 0.1719767062006107 No Hit CTAGATCGGTTGGGTCAGAGCCACATGGCCAAAGCTGTCAAGT 630 0.17008685228631829 No Hit CTAGATCGACTCTCTTTAAAAGGGAGAAAAGGCCTTGAAATCT 622 0.16792701924141262 No Hit CTAGATCGTGGCACCTCACTATAGTGAGTCGTATTACCGGCCG 620 0.16738706098018621 No Hit CTAGATCGTGGCAGCTCACTATAGTGAGTCGTATTACCGGCCG 584 0.1576678122781109 No Hit CTAGATCGTTAGAGAGTCGTATTACCGGCCGACGATCTAGTCT 579 0.15631791662504488 No Hit CTAGATCGAGTCGCGTATTACCGGCCGACGATCTAGCGACTGA 573 0.15469804184136568 No Hit CGATATCGGCCAAGTTGAAGATTCCCTTTCATAGAGCAGGTTT 566 0.15280818792707324 No Hit CTAGATCGTTAGACCGGCCGACGATCTAGTCTAAGATCGTCGG 559 0.15091833401278082 No Hit CTAGATCGTTAGATGAGTCGTATTACCGGCCGACGATCTAGTC 559 0.15091833401278082 No Hit CTAGATCGAGTCGCTCTGAACCCCTATAGTGAGTCGTATTACC 533 0.14389887661683753 No Hit CTAGAGAGGTATAGTGTAGGGTCTCTCTCCAGTGTGAATTCTC 527 0.14227900183315828 No Hit CTACATGGTGTGGCGGAGATCGTCGCCATGCCGAAGATGATCG 526 0.1420090227025451 No Hit CTAGATCGGCGCGCGGCCGACGATCTAGCGCGCGATCGTCGGA 525 0.14173904357193187 No Hit CTAGATCGTCGCACCGACGATCCAGACCTAGATCGTCGGACTG 519 0.14011916878825267 No Hit CTAGATCGAGTCGGAGTCGTATTACCGGCCGACGATCTAGCGA 499 0.1347195861759886 No Hit CTAGATCGTACGTGAACTCTGAACCCCTATAGTGAGTCGTATT 496 0.133909648784149 No Hit CTAGATCGAGTCGAGTCGTATTACCGGCCGACGATCTAGCGAC 485 0.13093987834740375 No Hit CTAGATCGAGTCGCTCCGACGATCTAGCGACTGATCGTCGGAC 478 0.12905002443311134 No Hit CTCGATCAGCCCGTTTGGGTCGTTTTGCGTGATTGATGAATCA 477 0.12878004530249812 No Hit CTAGATCGAGTCGTCGTATTACCGGCCGACGATCTAGCGACTG 471 0.1271601705188189 No Hit CTAGATCGGCGCGTTACCGGCCGACGATCTAGCGCGCGATCGT 466 0.12581027486575289 No Hit CTAGATCGCTACTACAGTCCGACGATCACTGTCTAGATCGTCG 460 0.12419040008207366 No Hit CTAGATCGTACGTGGCCGACGATCTAGACGTAGATCGTCGGAC 450 0.12149060877594163 No Hit CTAGATCGTACGTAACTCTGAACCCCTATAGTGAGTCGTATTA 450 0.12149060877594163 No Hit CTAGGTAGGTTTTGGTTCACAGAGAGTGTAAGATTCAAGACTT 449 0.12122062964532843 No Hit CTAGATCGTACGTACCCCTATAGTGAGTCGTATTACCGGCCGA 449 0.12122062964532843 No Hit CTAGATCGCAGGCGTCTCTAGCTGCATATATAGCAGAAGATGG 449 0.12122062964532843 No Hit CTAGATCGCAAGTAGTCCGACGATCTCATTCTAGATCGTCGGA 446 0.1204106922534888 No Hit CTAGATCGAGTCGATTACCGGCCGACGATCTAGCGACTGATCG 439 0.11852083833919638 No Hit CTAGATCGAGTCGGCGACTGATCGTCGGACTGTAGAACTCTGA 436 0.11771090094735677 Illumina DpnII expression Sequencing Primer (100% over 24bp) CTAGATCGTGGCATCTCACTATAGTGAGTCGTATTACCGGCCG 428 0.11555106790245114 No Hit CTAGATCGTACGTACGATCTAGACGTAGATCGTCGGACTGTAG 427 0.11528108877183793 No Hit CTAGATCAATTCGGATGACAATAATTTCTCCAGACTTTATTTG 426 0.11501110964122474 No Hit CTAGATCGGTAAGGGACAAGAGAAGATAACCAGGTGAATATTA 424 0.11447115137999833 No Hit CTAGATCGGTAATCAGTCCGACGATCACGCGCTAGATCGTCGG 409 0.11042146442080028 No Hit CTAGATCGATACACTATATGCACGATTCAAAAAGCTCACAAAG 404 0.10907156876773424 No Hit CTGGATCCCAATTCTGAGAGTTGTACATCTTGTTCCAAAGGCA 401 0.10826163137589463 No Hit CTAGATCGATGAAGTGAAATATGGCGAGGAAAACTGAAAAAGG 400 0.10799165224528143 No Hit CTAGATTGCTTGATCTTTACTGCTTGTGCAGAACAGCAGTGGA 398 0.10745169398405505 No Hit CTAGATCGTACGTCTCTGAACCCCTATAGTGAGTCGTATTACC 390 0.1052918609391494 No Hit CTAGATCGGTTGGGGTCAGAGCCACATGGCCAAAGCTGTCAAG 388 0.10475190267792299 No Hit CTAGATCGCTTCTAGACTAGATCGTCGGACTGTAGAACTCTGA 386 0.1042119444166966 Illumina DpnII expression Sequencing Primer (100% over 24bp) CTAGATCGTTAGAATTACCGGCCGACGATCTAGTCTAAGATCG 384 0.10367198615547019 No Hit CTAGATCGCTATTTTGGCTTCCCACCGTGATGGCAATGGACTG 382 0.10313202789424378 No Hit CTAGAACAAGTGTGTAGCAACTGCTGTGCAGATCAGCTCTGTC 371 0.10016225745749854 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.03536726611032967 0.0 0.0 14 0.0 0.0 0.1571278540168845 0.0 0.0 15 0.0 0.0 0.2913074819316467 0.0 0.0 16 0.0 0.0 0.3744610541605134 0.0 0.0 17 0.0 0.0 0.9751646197748914 0.0 0.0 18 0.0 0.0 1.259992602571821 0.0 0.0 19 0.0 0.0 1.473276115756252 0.0 0.0 20 0.0 0.0 2.0812691178971865 0.0 0.0 21 0.0 0.0 2.355297935469588 0.0 0.0 22 0.0 0.0 2.584510217360198 0.0 0.0 23 0.0 0.0 2.8355908088304775 0.0 0.0 24 0.0 0.0 3.206542134293019 0.0 0.0 25 0.0 0.0 3.654437511980324 0.0 0.0 26 0.0 0.0 4.005140402646876 0.0 0.0 27 0.0 0.0 4.4970423786241325 0.0 0.0 28 0.0 0.0 4.953307109360447 0.0 0.0 29 0.0 0.0 5.415511380970251 0.0 0.0 30 0.0 0.0 6.507306985170047 0.0 0.0 31 0.0 0.0 9.452779300160097 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTATCA 70 0.0 37.125256 37 TCTACCA 45 2.5247573E-9 37.125256 37 AACGAGG 25 1.2077409E-4 37.125256 37 CATACAC 35 5.470738E-7 37.125256 36 CATCATA 45 2.5247573E-9 37.125256 37 ACCCAAG 20 0.0018100287 37.125256 37 CACCCAA 20 0.0018100287 37.125256 36 TGAGTTG 30 8.106577E-6 37.12525 37 GTGTTCC 20 0.0018318249 37.03489 9 AGGTATA 190 0.0 37.03489 7 CGGCGTT 50 1.7644197E-10 37.03489 7 GGGGACC 25 1.2251924E-4 37.03489 8 CAGCGCG 20 0.0018318249 37.03489 7 GCCTTCA 20 0.0018318249 37.03489 8 GGAGCTA 65 0.0 37.03489 7 GCAAGTA 60 0.0 37.03489 8 GGTAATT 20 0.0018318249 37.03489 8 GGTAATG 45 2.586603E-9 37.03489 8 GGGCTTT 50 1.7644197E-10 37.03489 8 GGGCTTA 40 3.7896825E-8 37.03489 8 >>END_MODULE