FastQCFastQC Report
Thu 26 May 2016
SRR2099079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169723
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGATCGGCGCGAGGGAGTCGTATTACCGGCCGACGATCTAG63493.740801187817797No Hit
CTAGATCGTACGTGAGTCGTATTACCGGCCGACGATCTAGACG59083.4809660446727904No Hit
CTAGATCGTTGGTCCGGCCGACGATCTAGACCAAGATCGTCGG37112.1865038916351938No Hit
CTAGATCGTACGTGTGAGTCGTATTACCGGCCGACGATCTAGA35952.1181572326673463No Hit
CTAGATCGTGGTCACTCACTATAGTGAGTCGTATTACCGGCCG28021.6509253312750776No Hit
CTAGATCGAGTCGCCGACGATCTAGCGACTGATCGTCGGACTG25241.4871290278866154No Hit
CTAGATCGTTAGAAGTGAGTCGTATTACCGGCCGACGATCTAG22671.3357058265526771No Hit
CTAGATCGTACGTACCGGCCGACGATCTAGACGTAGATCGTCG22511.3262787011778014No Hit
CTAGATTGCTTGACTTTACTGCTTGTGCAGAACAGCAGTGGAA11790.6946613010611409No Hit
CTAGATCGTACGTCCGGCCGACGATCTAGACGTAGATCGTCGG10210.601568437984245No Hit
CTAGATCGTTAGATAAGATCGTCGGACTGTAGAACTCTGAACC9400.5538436157739375Illumina DpnII expression Sequencing Primer (100% over 27bp)
CTAGATCGTACGTCGGCCGACGATCTAGACGTAGATCGTCGGA9180.5408813183834836No Hit
CTAGATCGAGTCGGCCGACGATCTAGCGACTGATCGTCGGACT9120.5373461463679053No Hit
CTAGATCGAGTCGTCCGACGATCTAGCGACTGATCGTCGGACT8860.5220270676337326No Hit
CTAGATCGTGGCACTCACTATAGTGAGTCGTATTACCGGCCGA8160.4807833941186521No Hit
CTAGATCGTTAGACGACGATCGGCCCCTACAGTGAGTCGTATT8140.47960500344679274No Hit
CTAGATCGTACGTTACCGGCCGACGATCTAGACGTAGATCGTC7920.46664270605633884No Hit
CTAGATCGGCGCGATTACCGGCCGACGATCTAGCGCGCGATCG7800.45957236202518226No Hit
CTACATGGTGTGGGGAGATCGTCGCCATGCCGAAGATGATCGC7110.4189178838460315No Hit
CTAGATCGTACGTGGTGAGTCGTATTACCGGCCGACGATCTAG6840.403009609775929No Hit
CTAGATCGAGTCGGGCCGACGATCTAGCGACTGATCGTCGGAC6390.3764958196590916No Hit
CTAGATCGTACGTAGTGAGTCGTATTACCGGCCGACGATCTAG5960.3511604202141136No Hit
CTCAATCGACCGCCCGACGATCGGCCACTAGATCGTTTTTTTT5730.33760892748773No Hit
CTAGTTCAATAGAACGCCTGCGACTTCGCAGACCTTCTTCGAA5690.3352521461440111No Hit
CTAGATCGTACGTGGAGTCGTATTACCGGCCGACGATCTAGAC5590.3293601927847139No Hit
CTAGATCGTACGTATTACCGGCCGACGATCTAGACGTAGATCG5520.3252358254332059No Hit
CTAGATCGTTTCGGGGGGGGGCACTTAGGGAGGACAGATTGAC5330.31404111405054114No Hit
CTAGATCGACCTCGTACAGTGCTCTACGCCGGAAGTTCTACCA5280.31109513737089256No Hit
CTAGATCGAGTCGCCCGACGATCTAGCGACTGATCGTCGGACT5220.30755996535531427No Hit
CTAGATCGTACGTTTACCGGCCGACGATCTAGACGTAGATCGT5140.3028464026678765No Hit
CTAGATCGAGTCGCGGCCGACGATCTAGCGACTGATCGTCGGA5070.29872203531636843No Hit
CTAGATCGTACGTTGAGTCGTATTACCGGCCGACGATCTAGAC4850.2857597379259146No Hit
CTAGGTAGGTTTTGTTCACAGAGAGTGTAAGATTCAAGACTTC4800.28281376124626595No Hit
CTAGATCGTTAGACCGACGATCGGCCCCTACAGTGAGTCGTAT4560.26867307318395267No Hit
CTAGATCGTTAGAACCGGCCGACGATCTAGTCTAAGATCGTCG4560.26867307318395267No Hit
CTAGATCGTCGCCGGTCGGTCTCCATTCGTGAGAGCGGTTGGG4450.26219192448872575No Hit
CTAGATCGTTAGACGGCCGACGATCTAGTCTAAGATCGTCGGA4360.25688916646535825No Hit
CTAGATCGTTGCGCAGGATGCCAGGAGTTAAAGGCTCTGTCTC4130.2433376737389747No Hit
CTAGATCGGTAATCGCAAGACCAACAACGTAATCGCCATGGCT4030.23744572037967746No Hit
CTAGATCGTTAGAAGTCGTATTACCGGCCGACGATCTAGTCTA3780.22271583698143443No Hit
CTAGATCGAGTCGAGTGAGTCGTATTACCGGCCGACGATCTAG3510.206807562911332No Hit
CTAGATCGTTTCGGGGGGGGGGCACTTAGGGAGGACAGATTGA3470.2044507815676131No Hit
CTAGATCGTTAGACCGGCCGACGATCTAGTCTAAGATCGTCGG3410.2009156095520348No Hit
CTAGATCGTACGTTCGTATTACCGGCCGACGATCTAGACGTAG3410.2009156095520348No Hit
CTAGATCGTCGCCGTCGGTCTCCATTCGTGAGAGCGGTTGGGT3390.19973721888017537No Hit
CTAGATCGTTAGAGAGTCGTATTACCGGCCGACGATCTAGTCT3310.1950236561927376No Hit
CTAGATCGTTAGATGAGTCGTATTACCGGCCGACGATCTAGTC3260.19207767951308896No Hit
CTAGATCGTACGTAGTCGTATTACCGGCCGACGATCTAGACGT3240.19089928884122953No Hit
CTAGATCGTGACTCAGTCCGACGATCCCTAGCTAGATCGTCGG3150.18559653081786204No Hit
CTAGATCGAGTCGCCGGCCGACGATCTAGCGACTGATCGTCGG3040.17911538212263511No Hit
CTAGATCGTGGCAGCTCACTATAGTGAGTCGTATTACCGGCCG2960.17440181943519734No Hit
CTAGATCGATCCTGCCAGAGTACCCCACCCGTAGTGGCCTGGT2930.1726342334274082No Hit
CTAGATCGTGGCACCTCACTATAGTGAGTCGTATTACCGGCCG2930.1726342334274082No Hit
CTACATCGAGCTTGATGAGGTCCCTAGTGGAGCAGCAGTATCA2840.16733147540404072No Hit
CTAGATCGACTCTCTTTAAAAGGGAGAAAAGGCCTTGAAATCT2790.1643854987243921No Hit
CTAGATCGGTTGGGTCAGAGCCACATGGCCAAAGCTGTCAAGT2750.16202871738067323No Hit
CTAGATCGCGTAGCTACAGTCCGACGATCAGTTTCTAGATCGT2720.16026113137288406No Hit
CTACATGGTGTGGCGGAGATCGTCGCCATGCCGAAGATGATCG2680.15790435002916517No Hit
CGATATCGGCCAAGTTGAAGATTCCCTTTCATAGAGCAGGTTT2680.15790435002916517No Hit
CTAGATCGTACGTGAACTCTGAACCCCTATAGTGAGTCGTATT2540.14965561532614907No Hit
CTAGATCGAGTCGGAGTCGTATTACCGGCCGACGATCTAGCGA2490.14670963864650047No Hit
CTAGATCGGCGCGCGGCCGACGATCTAGCGCGCGATCGTCGGA2460.1449420526387113No Hit
CTAGATCGTACGTGGCCGACGATCTAGACGTAGATCGTCGGAC2410.1419960759590627No Hit
CTAGATCGAGTCGCGTATTACCGGCCGACGATCTAGCGACTGA2390.14081768528720326No Hit
CTAGATCGTTAGAATTACCGGCCGACGATCTAGTCTAAGATCG2380.14022848995127354No Hit
CTAGATCGTCGCACCGACGATCCAGACCTAGATCGTCGGACTG2310.1361041225997655No Hit
CTAGATCGTTAGAACAGTGAGTCGTATTACCGGCCGACGATCT2300.13551492726383577No Hit
CTAGATCGTACGTACCCCTATAGTGAGTCGTATTACCGGCCGA2240.13197975524825745No Hit
CTCGATCAGCCCGTTTGGGTCGTTTTGCGTGATTGATGAATCA2220.13080136457639802No Hit
CTAGATCGTACGTAACTCTGAACCCCTATAGTGAGTCGTATTA2220.13080136457639802No Hit
CTAGAGAGGTATAGTGTAGGGTCTCTCTCCAGTGTGAATTCTC2190.12903377856860887No Hit
CTAGATCGTACGTACGATCTAGACGTAGATCGTCGGACTGTAG2150.12667699722488998No Hit
CTAGATCGGCGCGTTACCGGCCGACGATCTAGCGCGCGATCGT2120.12490941121710081No Hit
CTAGATCGTTAGATTACCGGCCGACGATCTAGTCTAAGATCGT2070.1219634345374522No Hit
CTAGATCGAGTCGTCGTATTACCGGCCGACGATCTAGCGACTG2070.1219634345374522No Hit
CTAGATCGTGGCATCTCACTATAGTGAGTCGTATTACCGGCCG2050.12078504386559277No Hit
CTAGATCGTACGTCTCTGAACCCCTATAGTGAGTCGTATTACC2040.12019584852966303No Hit
CTAGATCGATACACTATATGCACGATTCAAAAAGCTCACAAAG2040.12019584852966303No Hit
CTAGATCGAGTCGCTCCGACGATCTAGCGACTGATCGTCGGAC2020.1190174578578036No Hit
CTAGATCGAGTCGCTCTGAACCCCTATAGTGAGTCGTATTACC2010.11842826252187388No Hit
CTAGATCGTTAGACGTATTACCGGCCGACGATCTAGTCTAAGA2010.11842826252187388No Hit
CTAGGTAGGTTTTGGTTCACAGAGAGTGTAAGATTCAAGACTT2010.11842826252187388No Hit
CTAGATCGCTACTACAGTCCGACGATCACTGTCTAGATCGTCG2000.11783906718594417No Hit
CTAGATCGTTGGTTCCGGCCGACGATCTAGACCAAGATCGTCG1990.11724987185001443No Hit
CTAGATCGCAAGTAGTCCGACGATCTCATTCTAGATCGTCGGA1990.11724987185001443No Hit
CTAGATCGGTAATCAGTCCGACGATCACGCGCTAGATCGTCGG1970.116071481178155No Hit
CTAGATCGCTGTGCGACGATCTTACTCTAGATCGTCGGACTGT1960.11548228584222528No Hit
CTAGATTGCTTGATCTTTACTGCTTGTGCAGAACAGCAGTGGA1930.1137146998344361No Hit
CTAGATCGCAGGCGTCTCTAGCTGCATATATAGCAGAAGATGG1910.11253630916257668No Hit
CTAGATCGGTAAGGGACAAGAGAAGATAACCAGGTGAATATTA1880.1107687231547875No Hit
CTAGATCGAGTCGATTACCGGCCGACGATCTAGCGACTGATCG1880.1107687231547875No Hit
CTAGATCGTTAGAACTCTGAACCCCTATAGTGAGTCGTATTAC1870.11017952781885779No Hit
CTAGATCAATTCGGATGACAATAATTTCTCCAGACTTTATTTG1860.10959033248292807No Hit
CTAGATCGAGTCGAGTCGTATTACCGGCCGACGATCTAGCGAC1850.10900113714699833No Hit
CTAGAACAAGTGTGTAGCAACTGCTGTGCAGATCAGCTCTGTC1850.10900113714699833No Hit
CTGGATCCCAATTCTGAGAGTTGTACATCTTGTTCCAAAGGCA1820.10723355113920918No Hit
CTAGATCGTTAGATACCGGCCGACGATCTAGTCTAAGATCGTC1800.10605516046734974No Hit
CTAGATACGTGTGGCCCAAGTTGATATTAGAATCATCAGCATG1770.10428757445956058No Hit
CTAGATCGGTTGGGGTCAGAGCCACATGGCCAAAGCTGTCAAG1770.10428757445956058No Hit
CTAGATCGTTGGTGCCGGCCGACGATCTAGACCAAGATCGTCG1770.10428757445956058No Hit
CTAGATCGCTGCGGCCTTGCTCTGCTTTCTCACACATCGAATG1750.10310918378770113No Hit
CTAGTTCGTTAGATAAGATCGTCGGACTGTAGAACTCTGAACC1730.1019307931158417Illumina DpnII expression Sequencing Primer (100% over 27bp)
CTAGATCGGCCTTATACACAAAAACTGCTATTATTTAGTGTAT1730.1019307931158417No Hit
CTAGATCGTACGTGTAGAACTCTGAACCCCTATAGTGAGTCGT1720.10134159777991199No Hit
CTAGATCGTACGTCCCTATAGTGAGTCGTATTACCGGCCGACG1700.10016320710805253No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGT251.2274677E-437.00884210
AGCCCGT501.7644197E-1037.0088428
GTAGACC200.001835362837.0088428
TGGCATC251.2274677E-437.0088429
AACGCCT950.037.00884213
GAGAACC251.2274677E-437.00884212
GGTGAGA200.001835362837.00884214
GTGTCGG251.2274677E-437.00884212
GCCTTAT251.2274677E-437.0088429
GGCACCT403.792411E-837.00884210
GGCATCT251.2274677E-437.00884210
CACACGG251.2274677E-437.0088429
AGCTCAT251.2274677E-437.0088429
CACACCC200.001835362837.0088429
GCCCACC200.001835362837.0088428
AGAAGTC501.7644197E-1037.00884211
TGCGTTA200.001835362837.00884210
TCCTATA200.001835362837.00884213
ACGTCTC251.2274677E-437.00884210
GTCGCTG200.001835362837.00884210
Produced by FastQC (version 0.11.5)