FastQCFastQC Report
Thu 26 May 2016
SRR2099078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609073
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCCAAGTGGGGGCTGGTGCGCTGTCTACCTTTCACTAC43850.7199465417117489No Hit
CTGACCCAAGTGGGGGGCTGGTGCGCTGTCTACCTTTCACTA23820.3910861259651963No Hit
CTGATGCCGGGAGTTATTTTCTGCTAGAAAGCAGACGTGTAA19250.31605406905247807No Hit
CTGATGCATTGAAAAGCTGCAAAGCTAGAGCAAGAGAGAAGA18340.3011133312427246No Hit
CTGATGCGCTGCGTGCAGATTCCGCAGGGCCTGACCTTCGGC17140.281411259405687No Hit
CTGATGCTCCAGGGTTCAAGCTGGAAAAATGCTTTGCCCCTC16750.2750080860586498No Hit
CTGATGCACTCAAGAAAAAGTTGGGCTGGGCATGGTGGCTCA16540.27156022348716824No Hit
CTGATGCATCCCCTGTAATCCCATCACTTTGGGAGGCCGAGG15990.262530107228526No Hit
CTGATGCACTCGCAGTCCGACGATCGGGGCTGATGCATTTTT15100.2479177372827231No Hit
CTGACGCACGTATGTCGTATACCCGAGAGAAATTGTGCGCGA14340.2354397584525993No Hit
CTGATGCGGGACTGCGCATGCAGTTGCACAGCGCCCTGAACC13480.22131994030272234No Hit
CTGATGCACGTTTGAGCCCAGGAGTTCAAGACCAGCCTGGGC13160.2160660544795123No Hit
CTGATCAAACAGCACGTACCGCGTCGATCACGGCTTCGTTGT13030.21393166336383324No Hit
CTGATGCAGTCGGCGCCCCATCCGCCTCGTGGTGCCGTTCCC12930.21228982404408012No Hit
CTGATGCACTTAATTCTTGACACCACCACCACCCTGCTTTAA12690.20834940967667256No Hit
CTGATGCCGGGAGGTTATTTTCTGCTAGAAAGCAGACGTGTA11740.1927519361390178No Hit
CTGATGCACTCTGGAGATCCGAGACCACCCTGGCTAACACGG11410.18733386638383248No Hit
CTGATGCAATGGAACCCAGAGTCGGAACATCACACTCTGGGG11080.18191579662864715No Hit
CTGATGCACAATTGCAGGCTTAGGATAGGCTAGTTTTGATCT10720.17600517507753585No Hit
CTGATGCTGGGTGGAGAGGAATAGATGAGGTTATGAAAACCA10330.16960200173049864No Hit
CTGACCCAAGTGTGGGGCTGGTGCGCTGTCTACCTTTCACTA10100.16582577129506645No Hit
CTGATGCCAAAAATAGTGTTTGCTATTGCACTTTTAGGTCTT9960.16352719624741205No Hit
CTGATGCATCCTTGATGCTGGGAAGACAGTGTATTTTTGAAT9520.15630310324049826No Hit
CTGATGCACACGGAACTGAGCGGCGCAGGCTGCCCCTGCTAC9370.15384034426086857No Hit
CTGATGCATTTGGCAACGACAGCACCCAAGGCCGTGCGCTGA9250.15187013707716482No Hit
CTGATGCAACTACGTTCGGATAGTTAGCCCGCAGGCTTTGGT8850.1453027797981523No Hit
CTGATGCAGATTGTGGCAGTAGCTGCCCAACTCAGGGTGTTT8770.14398930834234977No Hit
CTGATGCAAGTGTAGAGCTTGGGTTTCACCGTGTTAGCCAGG8660.1421832850906213No Hit
CTGATGCAGCGTGGGGAATCTATATATTCATTCATCCCTAAC8290.13610847960753475No Hit
CTGATGCAAGCCTCCGACGATCCTTTCTGATGCATCAGAGTC8240.13528755994765815No Hit
CTGATGCATACGTCCGACGATCCAGGCTGATGCATCAGTATA8120.1333173527639544No Hit
CTGATGCACTCTGAAGACTAGCCACCTGACTTGGTCTCTGAT8090.13282480096802846No Hit
CTGATGCATCACAAGGCACCTTACACAGACCCAAAACAAAAC7750.1272425472808678No Hit
CTGATGCATGCCCAATAATTAAACTGGTTTCTCACCAGCAAT7740.1270783633488925No Hit
CTGATGCATCGAGGGAGGCCAAGGAGGGCAGATCACTTGAGG7700.12642162762099124No Hit
CTGATGCACTACATAAGGAGTGGCACTTGGTGGTGAGGGCGG7620.12510815616518872No Hit
CTGATGCAATGGAATAAAACCCAGAGTCGGAACATCACACTC7560.12412305257333686No Hit
CTGATGCGGATTGGTCCAGGGTGACTTTGCTCAGGGGTAATT7550.12395886864136155No Hit
CTACTGCAACATTCAAGCCAAGTGGCTATGAGTGGGTAGAAT7410.12166029359370716No Hit
CTCATTCAACCGGCTCAACGGAGCCCGTGGAGCTGTCGTTGA7300.11985427034197872No Hit
CTGATGCAGTGGTTCTACAGTCCCAGTAGCAGGGAAGCATTT7290.11969008641000341No Hit
CTGATGCATTTGGCCCAGAGCTATCTGCAGGACCCCATCCTC6830.11213762553913897No Hit
CTGATGCACTGAGCCGAGGCTGGCAGATCACTTGAGCTCTGG6600.10836139510370679No Hit
CTGATGCATGCAACGAGGAATTCCCAGTAAGTGCGGGTCATA6580.10803302723975615No Hit
CTGATGCAGATTGGTGGCAGTAGCTGCCCAACTCAGGGTGTT6390.10491353253222521No Hit
CTGATGCATACCCTAGCTGGGACCACAGGTGCACACCACCAT6170.10130148602876832No Hit
CTGATGCATCGTGCATGTTGGCACATTGAGGCGGTTGCACAG6110.10031638243691643No Hit
CTGATGTTAGCATGTACCAGGAAGTTTGGTATATATTGTGGC6100.10015219850494111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCC200.002069510536.12957436
ACAACGA200.002103220236.0108458
GTCGCGC453.4087861E-936.0108459
GTATTCC200.002103220236.0108459
CTTCCGC200.002103220236.0108459
CATGCGG850.036.0108457
ATAGCGT200.002103220236.0108458
ACCGACA356.9551606E-736.0108458
CACGAGT200.002103220236.0108459
CACGAAT200.002103220236.0108457
AGTATCC404.859612E-836.0108458
GCCGATA404.859612E-836.0108459
CACTCGC2800.036.0108457
CGGATTA404.859612E-836.0108457
TGACACG404.859612E-836.0108459
ACTCGCT356.9551606E-736.0108458
ACTCGAA453.4087861E-936.0108458
GTATACG404.859612E-836.0108459
ACGTAGC200.002103220236.0108458
ATACGTC1400.036.0108458
Produced by FastQC (version 0.11.5)