FastQCFastQC Report
Thu 26 May 2016
SRR2099077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282455
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCCAAGTGGGGGCTGGTGCGCTGTCTACCTTTCACTAC20550.7275495211626631No Hit
CTGACCCAAGTGGGGGGCTGGTGCGCTGTCTACCTTTCACTA11100.3929829530367669No Hit
CTGATGCCGGGAGTTATTTTCTGCTAGAAAGCAGACGTGTAA9020.3193429041794268No Hit
CTGATGCATTGAAAAGCTGCAAAGCTAGAGCAAGAGAGAAGA8800.3115540528579774No Hit
CTGATGCACTCAAGAAAAAGTTGGGCTGGGCATGGTGGCTCA8160.2888955762864881No Hit
CTGATGCGCTGCGTGCAGATTCCGCAGGGCCTGACCTTCGGC8150.28854153759005857No Hit
CTGATGCTCCAGGGTTCAAGCTGGAAAAATGCTTTGCCCCTC7910.2800446088757501No Hit
CTGATGCATCCCCTGTAATCCCATCACTTTGGGAGGCCGAGG7810.2765042219114549No Hit
CTGATGCTGGGTGGAGAGGAATAGATGAGGTTATGAAAACCA7080.2506593970721No Hit
CTGATGCACTCGCAGTCCGACGATCGGGGCTGATGCATTTTT7010.24818112619709332No Hit
CTGACGCACGTATGTCGTATACCCGAGAGAAATTGTGCGCGA6780.24003823617921438No Hit
CTGATGCGGGACTGCGCATGCAGTTGCACAGCGCCCTGAACC6380.22587668832203361No Hit
CTGATGCACTTAATTCTTGACACCACCACCACCCTGCTTTAA6290.22269034005416793No Hit
CTGATGCACTCTGAAGACTAGCCACCTGACTTGGTCTCTGAT6250.22127418526844983No Hit
CTGATGCACTCTGGAGATCCGAGACCACCCTGGCTAACACGG6230.22056610787559078No Hit
CTGATCAAACAGCACGTACCGCGTCGATCACGGCTTCGTTGT6080.21525552742914802No Hit
CTGATGCACGTTTGAGCCCAGGAGTTCAAGACCAGCCTGGGC6030.21348533394700042No Hit
CTGATGCAGTCGGCGCCCCATCCGCCTCGTGGTGCCGTTCCC5820.20605052132198048No Hit
CTGATGCCGGGAGGTTATTTTCTGCTAGAAAGCAGACGTGTA5520.1954293604290949No Hit
CTGATGCAATGGAACCCAGAGTCGGAACATCACACTCTGGGG5060.17914358039333697No Hit
CTGATGCACAATTGCAGGCTTAGGATAGGCTAGTTTTGATCT4910.1738329999468942No Hit
CTGACCCAAGTGTGGGGCTGGTGCGCTGTCTACCTTTCACTA4880.17277088385760564No Hit
CTGATGCCAAAAATAGTGTTTGCTATTGCACTTTTAGGTCTT4600.16285780035757907No Hit
CTGATGCATTTGGCAACGACAGCACCCAAGGCCGTGCGCTGA4460.15790125860756582No Hit
CTGATGCAAGTGTAGAGCTTGGGTTTCACCGTGTTAGCCAGG4450.1575472199111363No Hit
CTGATGCAACTACGTTCGGATAGTTAGCCCGCAGGCTTTGGT4240.15011240728611636No Hit
CTGATGCATCCTTGATGCTGGGAAGACAGTGTATTTTTGAAT4130.14621798162539165No Hit
CTGATGCAGATTGTGGCAGTAGCTGCCCAACTCAGGGTGTTT4060.14373971075038502No Hit
CTGATGCACACGGAACTGAGCGGCGCAGGCTGCCCCTGCTAC4040.14303163335752597No Hit
CTGATGCATCGAGGGAGGCCAAGGAGGGCAGATCACTTGAGG3700.1309943176789223No Hit
CTGATGCATGCCCAATAATTAAACTGGTTTCTCACCAGCAAT3690.13064027898249278No Hit
CTGATGCATTTGGCCCAGAGCTATCTGCAGGACCCCATCCTC3680.13028624028606328No Hit
CTGATGCACTACATAAGGAGTGGCACTTGGTGGTGAGGGCGG3660.12957816289320423No Hit
CTACTGCAACATTCAAGCCAAGTGGCTATGAGTGGGTAGAAT3660.12957816289320423No Hit
CTGATGCATGACACGATCACGGCTGATGCATTTTTTTTTTTT3620.12816200810748615No Hit
CTGATGCATACGTCCGACGATCCAGGCTGATGCATCAGTATA3560.12603777592890905No Hit
CTGATGCAATGGAATAAAACCCAGAGTCGGAACATCACACTC3550.12568373723247953No Hit
CTCATTCAACCGGCTCAACGGAGCCCGTGGAGCTGTCGTTGA3480.12320546635747288No Hit
CTGATGCAGCGTGGGGAATCTATATATTCATTCATCCCTAAC3460.12249738896461383No Hit
CTGATGCATCACAAGGCACCTTACACAGACCCAAAACAAAAC3400.12037315678603672No Hit
CTTATGCAACGGAGAGAAGCACCAGGAGGGCGAGAAGGTGAA3360.11895700200031864No Hit
CGGGTGCAAGCTGTGCAGAGTCCTGATGCATTTTAGCTGATC3350.11860296330388911No Hit
CTGATGCGGATTGGTCCAGGGTGACTTTGCTCAGGGGTAATT3260.11541661503602343No Hit
CTGATGCAGTGGTTCTACAGTCCCAGTAGCAGGGAAGCATTT3220.11400046025030536No Hit
CTGATGCACTGAGCCGAGGCTGGCAGATCACTTGAGCTCTGG3160.11187622807172824No Hit
CTGATGCAAGCCTCCGACGATCCTTTCTGATGCATCAGAGTC3130.11081411198243966No Hit
CTGATGCAATGGGGAAACGAACAGAGTTCAATAGTGTCTTCA3020.10691968632171496No Hit
CTGATGCATATAGTTGGAGACGGCCACGCGCCACAAGATAGT3000.10621160892885592No Hit
CTGATGCATTTGCAACGACAGCACCCAAGGCCGTGCGCTGAA2920.10337929935741977No Hit
CTGATGCATCGTGCATGTTGGCACATTGAGGCGGTTGCACAG2890.10231718326813122No Hit
CTGATGCAACTAGGAAAAGGGGCTCCTGCAGATGGACCCCTT2880.10196314457170169No Hit
CTGATGCATGCAACGAGGAATTCCCAGTAAGTGCGGGTCATA2880.10196314457170169No Hit
CTGATGCGGATTGTCCAGGGTGACTTTGCTCAGGGGTAATTC2880.10196314457170169No Hit
CTGATGTTAGCATGTACCAGGAAGTTTGGTATATATTGTGGC2860.10125506717884264No Hit
CTGATGCATACCCTAGCTGGGACCACAGGTGCACACCACCAT2840.10054698978598361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG251.4417752E-436.0205516
GGCACGT356.9229645E-736.0205512
GCGTTCG251.4417752E-436.0205513
TAACATA251.4417752E-436.0205512
CTACGGT251.4417752E-436.0205518
CGCTAGC251.4417752E-436.0205512
GACTCCC309.963971E-636.0205516
CGAAGGC251.4417752E-436.0205512
CCTCGCG200.00209853736.02054614
CTCGCGG200.00209853736.02054615
GGTGAGC200.00209853736.02054613
GTAAAGG200.00209853736.02054613
GCTCAAC200.00209853736.02054613
CAGGTCG950.035.9950457
CAGCGTC251.4477968E-435.9950457
CAGCGTA251.4477968E-435.9950457
GTCGCAA502.401066E-1035.9950459
TCGCAAA301.0012494E-535.99504510
CACGGTA502.401066E-1035.9950457
CAAGTCG750.035.9950457
Produced by FastQC (version 0.11.5)