SRR2099076_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099076_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	549397
Sequences flagged as poor quality	0
Sequence length	43
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGTCTGCAGATAGCATGAAGCCAGCTTGAAATTTCAAATGTCA	3072	0.5591584955869799	No Hit
AGTCTGCAATACAGCCAGATTGGCGGTAACGTAGAGGCCGCAG	2051	0.3733183836096666	No Hit
AGTCTGCAATACAGGCCAGATTGGCGGTAACGTAGAGGCCGCA	1973	0.35912099993265345	No Hit
AGTCTGCACATTGTGCCTCTGTCTCCCAAGTGCTAGGATTAAA	1733	0.31543674246492065	No Hit
AGTCTGCAATGGACCGGCCTCACTATAGGGGTGCAGACTTCCA	1618	0.2945047024282987	No Hit
AGTCTGCAGGGCTGCGACATCGTCACGATCCACACGCCGCTCA	1545	0.2812174074485299	No Hit
AGTCTGCATGACCAAAGGGGACAAGGTGTGTTGGGTTGGAGGG	1454	0.26465379315868126	No Hit
AGCCTGGAGTACTCGACGATCGTACTAGCAGTTGACTCCAGGC	1234	0.2246098904799262	No Hit
AGTCTGCATAGGTAGAACCTTCCTCCTGAGGGAAAGTGGTTTT	1162	0.21150461323960634	No Hit
AGTCTGCAAGTATGTCCGACGATCACGCTAGTCTGCATTTTTT	1117	0.20331381496440643	No Hit
AGTCTGCATGTGGAGTTGAACATGGGTCAGTCGGTCCTGAGAG	938	0.1707326396030557	No Hit
AGTCTGCAGTGGGCCAAGATCACGGGCCTGAAGAACCCGGTCA	921	0.16763833803242464	No Hit
AGTCTGCAGTCTCAAATGCTGCAAATGGCCTTTGTTAAACTGC	890	0.16199578810950915	No Hit
AGTCTGCAGGTTTAAAGAACAAAGTGATGAACTGTACTTGATA	883	0.16072166393336695	No Hit
AGCCTGGAGTACTCGGCCGACGATCGTACTAGCAGTTGACTCC	881	0.16035762845446916	No Hit
AGTCTGCAGAGTCGAGTCACATCAGCTACTGCTTCTTGCGTCT	843	0.15344095435541147	No Hit
AGTCTGCAATTACTCTGCATTTTTTTTTTTTTTTTTTTTTTTT	839	0.15271288339761593	No Hit
AGTCTGCACATAAACAGGTCCGACGATCCGAGCAGTCTGCAGG	834	0.1518027947003715	No Hit
AGTCTGCACTGTGGAAAAGAAAAGCTTTGGGTGCCTACAAATT	827	0.1505286705242293	No Hit
AGCCTGGACGAGGTCCGACGATCTGGTGAGTCTGCATTTTTTT	826	0.1503466527847804	No Hit
AGTCTGCATCTCCCTAAGAGGGAAACTCATAGCTCTGAGTGCC	821	0.14943656408753597	No Hit
AGTCTGCAGTACTCGATCATCTCAGTCTGCATTTTTTTTTTTT	820	0.1492545463480871	No Hit
AGTCTACACTCCGGTCTGAGACCAGCCTGGTCAACATGGCAAA	817	0.14870849312974044	No Hit
AGTCTGCAATACACGTTACCGCCAGATTGGCGGTAACGTAGAG	812	0.14779840443249598	No Hit
AGTCTGCAAGTCTTGAAGGCCTTGGTCTGCTTGCCTGACACAT	805	0.14652428025635378	No Hit
AGTCTGCACGCCACAGGAAGACAGACGTACCTTCCTCACCACA	799	0.14543217381966048	No Hit
CGTCTGAATTGAGGCTGACGATGCAGCTGAACTGACCAGTGAC	779	0.14179181903068275	No Hit
AGTCTGACAAGATAGTGAGTCGTATTACCGGTTCAGAGTTCTA	746	0.13578523362886946	No Hit
AGTCTGCAAGTATGGTCCGACGATCACGCTAGTCTGCATTTTT	678	0.12340802734634518	No Hit
AGTCTGCAATACATGCCAGATTGGCGGTAACGTAGAGGCCGCA	676	0.1230439918674474	No Hit
AGTCTGCACTAATGCAGACTGGCGCGCCCCAGGGGTCTCTGGG	672	0.12231592090965185	No Hit
AGTCTGCAAAAGCCAACCAGGACTCATACCTTCCAGTTTGGGT	650	0.11831153064177635	No Hit
AGCCTGGAGTACTCCGGCCGACGATCGTACTAGCAGTTGACTC	647	0.11776547742342969	No Hit
AGTCTGCATCCTGCTACAGTCCGACGATCCGACTAGTCTGCAT	647	0.11776547742342969	No Hit
AGTCTGCAGGTCGGAGCGTGGCGATGTGTCGCCCGGCACGAGC	631	0.11485319359224751	No Hit
AGTCTGCACGACCGTCAAGGCTGGAGACCTGGGCGTGGACCTG	603	0.10975669688767867	No Hit
AGTCTGCATCCACCAGTCCGACGATCGTTACAGTCTGCCCCGA	602	0.10957467914822977	No Hit
AGTCTGCACAGCAATGCCACATGGAAGCATTCAGTCACTCCCG	580	0.10557028888035429	No Hit
AGTCTGCATCAGGGCCTGTAGTCCCAGGTACTCGGGAGGCTGA	571	0.10393212922531431	No Hit
AGTCTGCATGGCTGCAAACAGTGACTGCTTGTGGGCAGGTGGC	567	0.10320405826751876	No Hit
AGTCTGCACGTCAACAGTCCGACGATCAATGCAGTCTGCATTT	561	0.10211195183082544	No Hit
AGTCTGCAAGGGTCGGCATCCGACGAAAACAAGGTGCCGTAAG	551	0.10029177443633658	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATT	50	1.72804E-10	37.120155	36
CCCGGTC	110	0.0	37.120155	36
CGACGCT	55	1.2732926E-11	37.038933	9
GTTACGT	20	0.0018316142	37.038933	9
ATGCGCG	20	0.0018316142	37.038933	8
ACCGTTG	20	0.0018316142	37.038933	8
AGTACGG	45	2.588422E-9	37.038933	8
ACGAATC	40	3.7918653E-8	37.038933	8
GTGCCGG	25	1.2251786E-4	37.038933	9
AGCGCTA	35	5.578004E-7	37.03893	8
ACAACCG	20	0.001833255	37.03218	6
CAGGTCG	235	0.0	37.03218	7
CACGGTA	60	0.0	37.03218	7
CAACGTA	30	8.254698E-6	37.03218	7
CACGCGG	170	0.0	37.03218	7
CATACGT	20	0.001833255	37.03218	7
CATAACA	25	1.226494E-4	37.03218	7
CAAGTCG	115	0.0	37.032177	7
CACGAAT	180	0.0	37.032177	7
TGCGCGA	35	5.585989E-7	37.032177	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content