FastQCFastQC Report
Thu 26 May 2016
SRR2099075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250696
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGCAGATAGCATGAAGCCAGCTTGAAATTTCAAATGTCA13960.5568497303507036No Hit
AGCCTGGAGTACTCGACGATCGTACTAGCAGTTGACTCCAGGC11660.4651051472700003No Hit
AGTCTGCAATACAGCCAGATTGGCGGTAACGTAGAGGCCGCAG9880.39410281775536904No Hit
AGTCTGCAATACAGGCCAGATTGGCGGTAACGTAGAGGCCGCA9790.39051281233047197No Hit
AGCCTGGAGTACTCGGCCGACGATCGTACTAGCAGTTGACTCC8710.3474327472317069No Hit
AGTCTGCATGACCAAAGGGGACAAGGTGTGTTGGGTTGGAGGG8200.32708938315729014No Hit
AGTCTGCACATTGTGCCTCTGTCTCCCAAGTGCTAGGATTAAA7600.3031560136579762No Hit
AGTCTGCAATGGACCGGCCTCACTATAGGGGTGCAGACTTCCA7430.29637489229983727No Hit
AGTCTGCAGGGCTGCGACATCGTCACGATCCACACGCCGCTCA6520.2600759485592111No Hit
AGCCTGGAGTACTCCGGCCGACGATCGTACTAGCAGTTGACTC6440.25688483262596934No Hit
AGTCTGCAAGTATGTCCGACGATCACGCTAGTCTGCATTTTTT5080.2026358617608578No Hit
AGTCTGCATAGGTAGAACCTTCCTCCTGAGGGAAAGTGGTTTT5050.20143919328589208No Hit
AGTCTGCACGACCGTCAAGGCTGGAGACCTGGGCGTGGACCTG4980.19864696684430547No Hit
AGTCTGCATGTGGAGTTGAACATGGGTCAGTCGGTCCTGAGAG4720.18827584006126943No Hit
AGTCTGCAGTCTCAAATGCTGCAAATGGCCTTTGTTAAACTGC4560.1818936081947857No Hit
AGTCTGCACTAATGCAGACTGGCGCGCCCCAGGGGTCTCTGGG4360.17391581836168107No Hit
AGTCTGCAGGTTTAAAGAACAAAGTGATGAACTGTACTTGATA4190.16713469700354214No Hit
AGTCTGCAGTGGGCCAAGATCACGGGCCTGAAGAACCCGGTCA4170.16633691802023168No Hit
AGTCTGCAATTACTCTGCATTTTTTTTTTTTTTTTTTTTTTTT4110.16394358107030027No Hit
AGTCTGCATCCTGCTACAGTCCGACGATCCGACTAGTCTGCAT4080.16274691259533458No Hit
AGTCTGCACTGTGGAAAAGAAAAGCTTTGGGTGCCTACAAATT3950.15756134920381656No Hit
AGTCTGCAAGTCTTGAAGGCCTTGGTCTGCTTGCCTGACACAT3880.15476912276222995No Hit
AGCCTGGACGAGGTCCGACGATCTGGTGAGTCTGCATTTTTTT3840.15317356479560904No Hit
AGTCTGCAATACACGTTACCGCCAGATTGGCGGTAACGTAGAG3840.15317356479560904No Hit
AGTCTGACAAGATAGTGAGTCGTATTACCGGTTCAGAGTTCTA3790.15117911733733286No Hit
AGTCTGCACATAAACAGGTCCGACGATCCGAGCAGTCTGCAGG3780.15078022784567763No Hit
AGTCTGCAGAGTCGAGTCACATCAGCTACTGCTTCTTGCGTCT3740.1491846698790567No Hit
CGTCTGAATTGAGGCTGACGATGCAGCTGAACTGACCAGTGAC3720.14838689089574625No Hit
AGTCTACACTCCGGTCTGAGACCAGCCTGGTCAACATGGCAAA3700.14758911191243576No Hit
AGTCTGCATCTCCCTAAGAGGGAAACTCATAGCTCTGAGTGCC3670.14639244343747007No Hit
AGTCTGCAGTACTCGATCATCTCAGTCTGCATTTTTTTTTTTT3480.13881354309602068No Hit
AGTCTGCAATACATGCCAGATTGGCGGTAACGTAGAGGCCGCA3330.1328302007211922No Hit
AGTCTGCACGCCACAGGAAGACAGACGTACCTTCCTCACCACA3290.13123464275457125No Hit
AGTCTGCATAGGTGGAGAGGAGAGGAGGAGAGGAGAGGAGAGG3240.1292401952962951No Hit
AGTCTGCAAGTATGGTCCGACGATCACGCTAGTCTGCATTTTT3030.12086351597153525No Hit
AGTCTGCATGATCAAAAACGCACTAACTCTGGGATGTGTTGCG2950.1176724000382934No Hit
AGTCTGCACTGTAAGGAAGAATGGGGCTCTTCTCATCCCCTTT2950.1176724000382934No Hit
AGTCTGCAGGTCGGAGCGTGGCGATGTGTCGCCCGGCACGAGC2880.11488017359670677No Hit
AGTCTGCAAAAGCCAACCAGGACTCATACCTTCCAGTTTGGGT2870.11448128410505153No Hit
AGTCTGCATGGCTGCAAACAGTGACTGCTTGTGGGCAGGTGGC2830.11288572613843062No Hit
AGTCTGCACCATAGGACACCACAGGCTACCCGGAGTTGTTCTG2780.11089127868015446No Hit
AGTCTGCATCCACCAGTCCGACGATCGTTACAGTCTGCCCCGA2680.10690238376360214No Hit
AGTCTGCAAGGGTCGGCATCCGACGAAAACAAGGTGCCGTAAG2650.10570571528863643No Hit
AGTCTGCACACCCCTACAGTCCGACGATCTGTAGAGCTCTGAA2640.10530682579698121No Hit
AGTCTGCACGTCAACAGTCCGACGATCAATGCAGTCTGCATTT2640.10530682579698121No Hit
AGTCTGCACAGCAATGCCACATGGAAGCATTCAGTCACTCCCG2580.1029134888470498No Hit
AGTCTCCAAAGTGTCCGACGATCATTGTAGTCTGCTACGATTG2560.10211570986373936No Hit
AGTCTGCATCAGGGCCTGTAGTCCCAGGTACTCGGGAGGCTGA2550.10171682037208411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTCG1250.037.0041927
TGATATC1050.037.0041928
ACTGCGC308.279618E-637.0041923
CATATGG501.7826096E-1037.0041927
ATGATAG251.2300526E-437.0041928
CAGCCGA308.279618E-637.0041927
GCAACGA251.2300526E-437.0041926
ACATTGC251.2300526E-437.0041928
ATTAACG251.2300526E-437.0041928
CACGATT308.279618E-637.0041927
TTCCGCA308.279618E-637.00419230
CAACACT308.279618E-637.0041927
CATTCGA308.279618E-637.0041927
GCACCGT308.279618E-637.0041926
TGCGCGA600.037.0041925
AGTTGTA308.279618E-637.0041928
CAGGCGA452.5993359E-937.0041927
AATCTCG452.5993359E-937.0041928
AGCGATA251.2300526E-437.0041928
CATACTA251.2300526E-437.0041927
Produced by FastQC (version 0.11.5)