FastQCFastQC Report
Thu 26 May 2016
SRR2099074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196965
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGCACCGCGTGCGCGATCTTCGCCCGGCGCAGGCGCTGGA34771.7652882491813267No Hit
ATCGGTGACTGTTTGCGTCGGTCCAGGCTCCGTGCGAGTGTAA21811.107303328002437No Hit
ATCGGTACACTGAGCCGGCCAGTTGAGTTACAAAACATTTCTC16250.8250196735460615No Hit
ATCGGTCGCGCGGTCCGACGATCCAATCATCGGACTGTAGAAC15030.7630797349782956No Hit
ACCTGTACGTCAGACCTGGGCCATGGGAGGCTGGCGCTATGTT10310.5234432513390704No Hit
ATCGGTACATTCCGCACCAAATCAACCTGATAAAGGTTGATGA8420.4274871170004823No Hit
ATCGGCACCGCGTGGCGCGATCTTCGCCCGGCGCAGGCGCTGG6960.3533622724849592No Hit
ATCGGTACATCACGGTGCCCAATCCGCCTGAGTTAATCCATTG6920.35133145482699973No Hit
ATCGGTACGTATGCATGAAACACTGTCTCTTCTATAGGATCAT6790.34473129743863123No Hit
ATCGGTACGCGCATCGTATCATCGGTACTTTTTTTTTTTTTTT6300.3198537811286269No Hit
ATTGGCACAGTCTGGTCCTTTGTAGGGACATGGATGAAGCTGG6190.3142690325692382No Hit
ATCGGTACCCCGCTCCGCCCCCCGGGCCGCGGTTTTCCGCGCG5690.288883811844744No Hit
ATCGGTACCATCAGTGGGCTCCTCTGACCCCCTGAGCATGGTA5460.27720661031147664No Hit
ATCGGTACAAGGTATCTATTCCCACTCTTTATATAAAGCGTTA5410.2746680882390272No Hit
ATCGGTACGCTTAGCGGTACTTTTTTTTTTTTTTTTTTTTTTT4940.25080598075800264No Hit
ATCGGTACGGAGAGACTGCAGCATTTCATAATGTTTGTCTAGC4650.23608255273779608No Hit
ATCGGTACGGACCGTCCGACGATCGAGGTATCGGTATACTGAT4600.23354403066534662No Hit
ATCGGATCTCGAGCAGTCCGACGATCTCGACGTAAAAGACAGT4390.22288223796105908No Hit
ATCGGTACCATCAGGTGGGCTCCTCTGACCCCCTGAGCATGGT4270.21678978498718046No Hit
ATCGGTACGATTCAATGGACTCGGGTCATGATAAGGGATTGGG4200.2132358540857513No Hit
ATCGGTACAGCAACAGTCCGACGATCCGTCGATCGGACTGTAG4140.21018962759881196No Hit
ACCTGTACGTCAGGACCTGGGCCATGGGAGGCTGGCGCTATGT4030.20460487903942326No Hit
ATCGGAATCTGCACAACACGGGAAACCTCACCCGGCCCGGACA4000.2030817657959536No Hit
ATCGGTACAGGGCGCCTCAGCTTCCTCAACTGTACCTCACAGG3760.19089685984819638No Hit
ATCGGTACAGGGCGGCCTCAGCTTCCTCAACTGTACCTCACAG3610.1832812936308481No Hit
ATCGGTACGCTTACGGTACTTTTTTTTTTTTTTTTTTTTTTTT3610.1832812936308481No Hit
ATCGGTACGCATCAAACACAGTGGCCTCCTACCGTGGCTATGT3530.17921965831492903No Hit
ATCGGTACCGATCAGTCCGACGATCTTGGCATCGGTCTGTAGA3530.17921965831492903No Hit
ATCGGTACACTGAGGCCGGCCAGTTGAGTTACAAAACATTTCT3410.17312720534105044No Hit
ATCGGCACCGCGTCGCGCGATCTTCGCCCGGCGCAGGCGCTGG3330.16906557002513137No Hit
ATCGGTACATCACGGGTGCCCAATCCGCCTGAGTTAATCCATT3320.16855786561064148No Hit
ATCGGTACGTTGCGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3160.16043459497880336No Hit
ATCGGTACTCGACGTCCGACGATCCCATCATCGGACTGTAGAA3100.15738836849186402No Hit
AACGGTAGGTCATGGACCATCCTGGCAATAGTCCTCAGTTGCA3020.15332673317594497No Hit
ATCGCTATGTCAGGTCCGACGATCGTCATACAGTGTGGTACCT3000.1523113243469652No Hit
ATCGGTACAGCTCCAGTCCGACGATCTGATCATCGGACTGTAG3000.1523113243469652No Hit
ATCAGTACGAGGCCGAACCATGCCGCATCTGCATGGTTCGTGT2930.148757393445536No Hit
ACCTGTACGTCAGCACCTGGGCCATGGGAGGCTGGCGCTATGT2830.14368034930063717No Hit
ATCGGAATCTGCACCAACACGGGAAACCTCACCCGGCCCGGAC2790.14164953164267763No Hit
AACGGTAGGTCATGACCATCCTGGCAATAGTCCTCAGTTGCAG2780.14114182722818774No Hit
ATCGGTACTCATAGGAGAGGAGAGATAACTACGTTCCTGAGGT2770.14063412281369786No Hit
ATCGGTACAGTTAGTCCGACGATCATTCGATCGGACTGTAGAA2760.14012641839920797No Hit
ATCGGTACGCTTATCGGTACTTTTTTTTTTTTTTTTTTTTTTT2680.1360647830832889No Hit
ATCGGTACTCACACTACAGTCCGACGATCACTTTATCGTCGGA2660.13504937425430913No Hit
ATCGGTACTTCCCACTGAAAATCACGGAAAATGAGAAATACAA2650.13454166983981927No Hit
ATCGGTACAGTGCTCCGACGATCCAATCATCGGACTGTAGAAC2620.13301855659634962No Hit
ATCGGTACCGTGACAGTCCGACGATCGTCGGACTGTAGAACTC2590.13149544335287996Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATCGGTACTCTACACTTGCTGCCAAGTCAGAGGACATCAGTCC2540.12895692128043054No Hit
ATCGGTACTCCCGGCCAACCTGGGTAGGTGGATGAGGGGCCTC2520.12794151245145077No Hit
ATCGGTACAGCTGGCGGGGAAGGATCAACGCTTATACAGACCG2470.12540299037900132No Hit
ATCGGTACGCTTACCGGTACTTTTTTTTTTTTTTTTTTTTTTT2430.12337217272104181No Hit
ATCGGAATCTCGACCAACACGGGAAACCTCACCCGGCCCGGAC2400.12184905947757216No Hit
ATCGGTACACTGACGCCGGCCAGTTGAGTTACAAAACATTTCT2360.11981824181961263No Hit
ATCTGTATATGGCTGTGGAAAATCTGGAACTTTCCACATTTTT2340.11880283299063284No Hit
ATCAGTACGAGGCGCCGCATCTGCATGGTTCGTGTCCAGATAG2330.11829512857614297No Hit
ATCGGTACGGCTTTGAGTAACAAGAATTCCAATTCTCCAGGCA2250.1142334932602239No Hit
ATCGGTACGAAAAAAGAAGTGAAGCAACAAACAGCTTCACTTC2240.113725788845734No Hit
ATCGGTACAAGGTGATCTATTCCCACTCTTTATATAAAGCGTT2200.11169497118777448No Hit
ATCGGTACCCACAGGTGTGCTGGATACCCCAGCACACCCAAAA2190.11118726677328458No Hit
ATCGGTACGTTGGCAGTCCGACGATCTTCTGATCGTCGGACTG2190.11118726677328458No Hit
ATCAGTACGAGGCCGCCGCATCTGCATGGTTCGTGTCCAGATA2160.10966415352981493No Hit
ATCGGTACATGTATCCGCCCCCCGGGCCGCGGTTTTCCGCGCG2090.10611022262838575No Hit
ATAGGTTCAGCTAGGGAAACTTTGCCAGGGCACCAAGGACTTG2080.10560251821389588No Hit
ATCGGTGACTGTTCTGCGTCGGTCCAGGCTCCGTGCGAGTGTA2020.10255629172695657No Hit
ATCGGTACGGAAGGCAGAGTTTTGGATTTCAATTTACACATAT1980.10052547406899702No Hit
ATTGGCACAGTCTCCATGTCCTTTGTAGGGACATGGATGAAGC1970.10001776965450715No Hit
GTAGGTACAGTCTCCGACGATCAGTCCAAAAGAAGAGCTTGCA1970.10001776965450715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGTT850.037.1614837
GAAATAT200.001799322537.1614837
ATAATAT308.033259E-637.1614836
GCTGCTA355.4118027E-737.1614837
AGTGTCT251.1987741E-437.1614836
CCCTGCC501.7644197E-1037.0104188
CAGCGAT251.2278755E-437.0104188
TCTGACG251.2278755E-437.01041813
AGTCATG200.00183569337.0104189
TGCTTCG403.796231E-837.0104189
CACGGCA200.00183569337.0104188
CAACGCC251.2278755E-437.0104188
CGCGGCC251.2278755E-437.0104188
CATTGCG251.2278755E-437.01041813
CGTGACA200.00183569337.0104189
ATTACGC200.00183569337.0104189
TGGCGGA200.00183569337.0104189
CTCCCGG452.590241E-937.0104188
CCGGACG200.00183569337.0104189
CCGCTCT200.00183569337.0104188
Produced by FastQC (version 0.11.5)