##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099074_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 196965 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.24818622597923 35.0 35.0 37.0 31.0 37.0 2 34.120498565735026 35.0 35.0 37.0 30.0 37.0 3 34.9953189652984 37.0 35.0 37.0 32.0 37.0 4 35.8703119843627 39.0 35.0 39.0 30.0 39.0 5 35.38037722437996 39.0 35.0 39.0 27.0 39.0 6 34.48244611986901 37.0 34.0 39.0 26.0 39.0 7 34.95283425989389 37.0 34.0 39.0 27.0 39.0 8 35.74655395628665 37.0 35.0 39.0 31.0 39.0 9 30.62616708552281 31.0 31.0 33.0 27.0 34.0 10 30.984895793668926 31.0 31.0 34.0 27.0 34.0 11 30.950722209529612 31.0 31.0 34.0 27.0 34.0 12 34.41014901124565 37.0 35.0 37.0 30.0 37.0 13 34.351763003579315 37.0 35.0 37.0 30.0 37.0 14 31.60598075800269 31.0 31.0 34.0 30.0 34.0 15 31.624425659381107 31.0 31.0 34.0 30.0 34.0 16 31.688437031959992 31.0 31.0 34.0 30.0 34.0 17 35.244530754194905 35.0 35.0 37.0 33.0 37.0 18 35.292534206584925 35.0 35.0 37.0 33.0 37.0 19 34.792374279694364 35.0 35.0 37.0 31.0 37.0 20 34.41589622521768 35.0 35.0 37.0 30.0 37.0 21 34.35393090142919 35.0 35.0 37.0 30.0 37.0 22 35.79155179854289 38.0 35.0 39.0 30.0 39.0 23 35.892417434569595 38.0 35.0 39.0 30.0 39.0 24 36.031223821491125 38.0 35.0 39.0 30.0 39.0 25 36.022349148325844 38.0 35.0 39.0 30.0 39.0 26 35.8940014723428 38.0 35.0 39.0 30.0 39.0 27 37.170984692711905 39.0 36.0 41.0 31.0 41.0 28 37.26163531591907 39.0 36.0 41.0 31.0 41.0 29 37.32178813494783 39.0 36.0 41.0 31.0 41.0 30 37.40298022491306 39.0 36.0 41.0 31.0 41.0 31 37.543710811565504 40.0 36.0 41.0 32.0 41.0 32 37.758028076054124 40.0 36.0 41.0 32.0 41.0 33 37.828751300992565 40.0 36.0 41.0 32.0 41.0 34 37.923844337826516 40.0 37.0 41.0 33.0 41.0 35 37.901708425354755 40.0 37.0 41.0 33.0 41.0 36 37.929611859975125 40.0 37.0 41.0 33.0 41.0 37 37.9377503617394 40.0 37.0 41.0 33.0 41.0 38 37.9441626684944 40.0 37.0 41.0 34.0 41.0 39 37.91382732972863 40.0 37.0 41.0 34.0 41.0 40 37.875119945167924 40.0 37.0 41.0 33.0 41.0 41 37.76280557459447 40.0 37.0 41.0 33.0 41.0 42 37.53743050795827 40.0 37.0 41.0 33.0 41.0 43 37.48182164343919 40.0 36.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6301609578897782 1101 2 0.7137196395971017 1101 3 0.6691662119041979 1101 4 0.8870355486127295 1101 5 0.9691040600126684 1101 6 1.101336321501762 1101 7 1.2385852476438899 1101 8 1.1005854980210472 1101 9 0.9872452583608791 1101 10 0.960010663418899 1101 11 0.8357556542566336 1101 12 0.9091805630532903 1101 13 0.9066243876268487 1101 14 0.13177682291324544 1101 15 0.13366328086270585 1101 16 0.04793525515989927 1101 17 0.16010501818106349 1101 18 0.14857128333576242 1101 19 -0.10675052306179822 1101 20 -0.3775829046304082 1101 21 -0.21695547246731905 1101 22 -0.29600334832307595 1101 23 -0.7160232640030202 1101 24 -0.3745246904786512 1101 25 -0.531956150204195 1101 26 -0.8085827858567995 1101 27 -0.7059071176028553 1101 28 -0.37644967688599706 1101 29 -0.2530019265751804 1101 30 -0.1453971499186082 1101 31 -0.2695119438507163 1101 32 -0.1537649538648722 1101 33 -0.13534775872419402 1101 34 0.07299755015519338 1101 35 0.08110718485599477 1101 36 0.06312066081342493 1101 37 0.22049156231656752 1101 38 0.20372889679051553 1101 39 0.15378580924183183 1101 40 0.4408039228831484 1101 41 0.6626067557421891 1101 42 0.8495258788968201 1101 43 0.7309191771762968 1102 1 0.5348174833481778 1102 2 0.7612147115649677 1102 3 0.47219369730606786 1102 4 0.7554663887957176 1102 5 0.9097073255313219 1102 6 0.9202450878200636 1102 7 0.8018500224421174 1102 8 0.7811560339677897 1102 9 0.6839388277438516 1102 10 0.6951056907555326 1102 11 0.796777709524978 1102 12 0.7667325398450444 1102 13 0.9933949458892073 1102 14 0.00686515556471079 1102 15 0.21111706893873006 1102 16 0.060524982537444316 1102 17 0.12142461501225199 1102 18 0.15712560326732472 1102 19 0.16334283060354693 1102 20 0.17829912013992555 1102 21 -0.7015762929381779 1102 22 -0.3561067440161665 1102 23 -0.07746187745933497 1102 24 -0.07904732223377664 1102 25 -0.19052720684867097 1102 26 -0.2765598751564653 1102 27 -0.6120885435707919 1102 28 -0.5648306788572057 1102 29 -0.3435103507206776 1102 30 -0.4986347740492292 1102 31 -0.3109260101280995 1102 32 -0.3526156995784149 1102 33 -0.006326298538972708 1102 34 -0.12326830323119964 1102 35 -0.05228441703339115 1102 36 -0.12237790726780418 1102 37 0.0035651666606781873 1102 38 0.07418673887033123 1102 39 -0.08396849581265542 1102 40 -0.01923252595829439 1102 41 -0.20474549627551397 1102 42 -0.03599500480162021 1102 43 -0.28053617299293165 1103 1 0.4531423243494004 1103 2 0.3224152917710157 1103 3 0.2405947833327744 1103 4 0.04852623184254412 1103 5 0.13680592336670117 1103 6 0.5454357003837558 1103 7 0.44044860168115463 1103 8 0.1937531998844051 1103 9 0.43755581736709104 1103 10 0.46001066341889896 1103 11 0.577991679101352 1103 12 0.3688078922458331 1103 13 0.2855063752044913 1103 14 0.1845718539691461 1103 15 0.16471918148382514 1103 16 0.1845812178928199 1103 17 0.18805532874007014 1103 18 0.12062097277675576 1103 19 0.1013240110996918 1103 20 -0.1539804201583621 1103 21 -0.12378777060396118 1103 22 -1.1345126650932613 1103 23 -0.5017375497173049 1103 24 -0.49564270290101575 1103 25 -0.8083536657321417 1103 26 -0.28373806535990553 1103 27 -0.5941058753668287 1103 28 -0.6186857017307119 1103 29 -0.523188261978909 1103 30 -0.39694994494966807 1103 31 -0.12044362086935223 1103 32 -0.11960346318164738 1103 33 -0.10739744816518737 1103 34 -0.010853381521819472 1103 35 0.012784203489538015 1103 36 0.19355544342212028 1103 37 -0.059011543273491895 1103 38 0.30931895890231687 1103 39 0.05751251731636131 1103 40 -0.09025197773796378 1103 41 0.013538432760825003 1103 42 0.2718861273440254 1103 43 0.13775147531294607 1104 1 0.12702772162533194 1104 2 0.19540863825164934 1104 3 0.06460827467950736 1104 4 0.2900618862281519 1104 5 0.414806918629985 1104 6 0.14850074268244384 1104 7 0.19530051019065553 1104 8 0.11509053884527276 1104 9 0.3370065794807324 1104 10 0.2557144435762986 1104 11 0.3571998918665251 1104 12 0.48938219602230504 1104 13 0.3524009694503931 1104 14 0.031181508022999793 1104 15 -0.001093524710768179 1104 16 0.131039739373918 1104 17 -0.0717913269909829 1104 18 -0.3423146852950154 1104 19 -0.355225374233072 1104 20 -0.23342639026359535 1104 21 -0.31585515361445005 1104 22 -0.10191779254225963 1104 23 0.10474640626994614 1104 24 0.01601477908288018 1104 25 -0.17128379456345044 1104 26 -0.05971167245208164 1104 27 -0.2135651156491889 1104 28 -0.06680299535181433 1104 29 -0.10326087933703576 1104 30 -0.39681171393799985 1104 31 -0.03372669955044216 1104 32 -0.22875066999367277 1104 33 0.08384054877529934 1104 34 -0.1241567340846288 1104 35 0.029353494087985155 1104 36 -0.28612459786287303 1104 37 -0.13504781509095665 1104 38 -0.32619350953493864 1104 39 -0.3314142571090528 1104 40 0.03259853003061863 1104 41 0.07248935153628366 1104 42 -0.05761052746549211 1104 43 0.047587994103146514 1105 1 0.11463300757921502 1105 2 -0.009882844874951502 1105 3 0.08531528022718504 1105 4 0.24417840575558358 1105 5 0.4007810786462045 1105 6 0.08270278112909324 1105 7 0.21374052714699587 1105 8 0.026051336530365177 1105 9 -0.059338592570799875 1105 10 0.2581473093816342 1105 11 0.22084882195849787 1105 12 0.0427209357240983 1105 13 0.2140778037759219 1105 14 0.27152837570827515 1105 15 0.3137875044651892 1105 16 0.2404818390108332 1105 17 0.29364539085187147 1105 18 0.34111786718668924 1105 19 0.39014388687608914 1105 20 0.3398083997174197 1105 21 0.41658490020348893 1105 22 0.5673506889440034 1105 23 0.4423618291646818 1105 24 0.09752499896234212 1105 25 0.3748761479324543 1105 26 0.25352901538543904 1105 27 0.12017983891888662 1105 28 0.5769664721823275 1105 29 0.23457571317637615 1105 30 0.08752210473977584 1105 31 0.08763091329213779 1105 32 0.32760150576245195 1105 33 0.050987645002514626 1105 34 0.18790438245333263 1105 35 0.10905749541500143 1105 36 0.21529457385690876 1105 37 0.36645429523582607 1105 38 0.11677237505138294 1105 39 0.11341313843437462 1105 40 0.3755865315788043 1105 41 0.4203707308974671 1105 42 0.4054265000148334 1105 43 0.2029688666172902 1106 1 0.38028040366139493 1106 2 0.13527133205180064 1106 3 0.14731682383407474 1106 4 0.3813389041200992 1106 5 0.4657963309524291 1106 6 0.2858157217002315 1106 7 0.45803469451808354 1106 8 0.5505285315761199 1106 9 0.6448217955360782 1106 10 0.6247906994363959 1106 11 0.4185223858527962 1106 12 0.5518723194574164 1106 13 0.7882076872703507 1106 14 0.18317525725482398 1106 15 0.14498306475774214 1106 16 -0.04888932494227305 1106 17 -0.010187899603486983 1106 18 0.04544364174658 1106 19 0.07554210070239975 1106 20 -0.16588824477521058 1106 21 -0.17401012350190115 1106 22 -0.23416719116919182 1106 23 -0.25042364622948554 1106 24 -0.487189617524848 1106 25 -0.5229334003787685 1106 26 -0.6450890889182901 1106 27 -0.621743789292367 1106 28 -0.6946715887533941 1106 29 -0.34365207909824136 1106 30 -0.1423650649467305 1106 31 -0.020329687979497635 1106 32 -0.07651110403577377 1106 33 0.07803751820623006 1106 34 0.25227646522006353 1106 35 0.2485517803942372 1106 36 0.3660535139135064 1106 37 0.4958232540362246 1106 38 0.3259494855662908 1106 39 0.1107485789136291 1106 40 0.05657443999145784 1106 41 0.2831367274427308 1106 42 0.26958909327441916 1106 43 -0.03368077137013614 1107 1 0.10068574682122744 1107 2 -0.014234390231443683 1107 3 -0.1775069014963293 1107 4 -0.11579109623422568 1107 5 -0.2938854888399405 1107 6 -0.13664113972485126 1107 7 -0.281982312887223 1107 8 -0.3475864810022671 1107 9 -0.34899530399267675 1107 10 -0.026451195472013467 1107 11 -0.20322229047502205 1107 12 -0.4608123703345939 1107 13 -0.3748685271646863 1107 14 0.1745298262233952 1107 15 0.11830269768124424 1107 16 0.15932737774702588 1107 17 -0.040252031694336665 1107 18 0.20203578290804103 1107 19 -0.0851601634084318 1107 20 0.02261049738543619 1107 21 0.0648907729300845 1107 22 0.15287529191198246 1107 23 0.46146027822928914 1107 24 0.03472513285604606 1107 25 -0.24741343439357877 1107 26 -0.03404490509657876 1107 27 -0.24083105854085574 1107 28 -0.6247109183781632 1107 29 -0.5980013686847485 1107 30 -0.6722874686600093 1107 31 -0.20613559096642575 1107 32 -0.18495102891973403 1107 33 -0.2727933644072422 1107 34 0.221043074014311 1107 35 0.2111886368588216 1107 36 0.1351071226723164 1107 37 0.04548133432534485 1107 38 0.4065220682116504 1107 39 0.35016324071428784 1107 40 0.03465968961056376 1107 41 0.01681138995203213 1107 42 0.30232834849179113 1107 43 0.17455364736635204 1108 1 -0.00273241685142267 1108 2 -0.19945323824968852 1108 3 0.09747884130378281 1108 4 0.034551076563040795 1108 5 0.16410923599610072 1108 6 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1109 34 0.24017399645944693 1109 35 0.3781929967191644 1109 36 0.2950922882927358 1109 37 0.07218321756792534 1109 38 0.07699079842493717 1109 39 0.12566661463110762 1109 40 -0.03272819323401421 1109 41 0.41631751365628133 1109 42 0.5026286542247362 1109 43 0.28871505265245645 1110 1 -0.26731575018475695 1110 2 -0.37787585729731177 1110 3 -0.19240211107716476 1110 4 -0.048931066294102266 1110 5 -0.3945217163848511 1110 6 -0.44528634930568955 1110 7 -0.6130451871387166 1110 8 -0.11936791812802028 1110 9 -0.4339503938130349 1110 10 -0.49338607571153403 1110 11 -0.7501721407744242 1110 12 -0.5170810829094421 1110 13 -0.023053407404205473 1110 14 -0.26055625162091545 1110 15 -0.25845628435468626 1110 16 -0.23185899949848476 1110 17 -0.23420787001769128 1110 18 -0.4518751452356682 1110 19 -0.17144384604316087 1110 20 -0.046158215189414875 1110 21 -0.48785997557290983 1110 22 -0.4147145284472984 1110 23 -0.12710633853718178 1110 24 -0.13582127432958657 1110 25 0.3992744398579191 1110 26 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-0.016241393195848275 2216 35 -0.17680645729022615 2216 36 -0.0728143837125046 2216 37 -0.15363849829856235 2216 38 -0.15487321351405825 2216 39 -0.09724315132091732 2216 40 -0.050290891155611916 2216 41 0.4219048175546547 2216 42 0.5117648970813633 2216 43 0.265492252084627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 23.0 26 151.0 27 625.0 28 1476.0 29 2746.0 30 5000.0 31 7823.0 32 12158.0 33 17824.0 34 23061.0 35 25367.0 36 27797.0 37 39742.0 38 33170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.865649211523035 98.02956915547162 0.34524426031417227 0.759537372691179 2 0.7874655388630237 1.8628053269428975 95.56003472768721 1.7896944065068718 3 0.8768099754269817 1.3073455047622915 1.9638106456002113 95.85203387421052 4 95.53725553908329 1.8079445990130176 2.2851891716252717 0.3696106902784265 5 95.85761865112407 1.6926951117970797 1.0123677423285473 1.4373184947503097 6 1.4637192583416259 6.0173432708515255 87.0103166060803 5.508620864726549 7 3.0771964562231866 91.91023785951819 1.186505216662859 3.8260604675957657 8 2.7472164822834744 3.309250976071648 4.7780547615542 89.16547778009067 9 23.274289054359702 28.48306183130448 18.442654197057102 29.79999491727871 10 34.51215982205882 13.059176615765875 26.514455182079942 25.91420838009537 11 23.319840935292355 18.874967623323396 26.13648483247927 31.668706608904984 12 33.93786725308658 17.334091751692473 25.37925150202386 23.34878949319709 13 19.969324062834882 27.8470469331681 23.166427116715845 29.017201887281168 14 36.55892136200899 22.16336998197192 18.65068684457761 22.627021811441487 15 27.656571298012235 23.77852809017288 22.63816607854586 25.92673453326902 16 23.303632625085672 29.58698245881248 23.094458406315844 24.014926509786 17 28.324829284390628 23.834183738227605 23.260477749854036 24.580509227527735 18 23.520930114487346 31.90109918005737 21.31749295560125 23.260477749854036 19 26.840426936396227 28.392692116300562 17.208461546273448 27.558419401029767 20 24.29045786411316 24.50573218655754 23.647681231531596 27.5561287177977 21 21.80031985378113 26.534155814484805 24.962810651638616 26.702713680095446 22 19.991369024953674 23.265554793998934 28.92188967583073 27.821186505216662 23 21.121011347193665 23.28332444850608 29.70223136090168 25.89343284339857 24 21.390602391287793 26.882948747239354 26.307719645622317 25.418729215850533 25 22.833498337268043 26.46155408321275 26.920011169497123 23.784936410022084 26 23.81143379366369 24.774573517465477 28.13667749796913 23.277315190901707 27 22.990886705759905 25.2146320412256 27.590180996623765 24.204300256390727 28 21.367755692635747 25.08313659787272 28.83761074302541 24.71149696646612 29 22.539537481278398 24.02406518924682 30.76079506511309 22.67560226436169 30 22.709110755718022 26.672759119640542 29.429594090320617 21.18853603432082 31 21.4002487751631 25.092782981748023 28.722869545350697 24.784098697738177 32 23.79965983804229 24.918640367577996 28.2258269235651 23.05587287081461 33 22.780697078161094 24.67849617952428 32.2960932145305 20.244713527784125 34 21.78711953900439 28.62945193308456 31.10552636255172 18.47790216535933 35 23.805752291016173 26.45139999492296 29.366638742923868 20.376208971137004 36 24.959256720737187 25.55225547686137 29.712893153605968 19.775594648795472 37 22.260526088656245 26.175474581520387 28.4987535730061 23.06524575681727 38 22.52684487091615 25.60048739623791 30.71865559871043 21.154012134135506 39 21.690655700251313 26.009697154316758 32.69311806666159 19.60652907877034 40 22.8893029584537 26.25037443960987 32.094170927239404 18.76615167469702 41 24.10491257285595 25.54883125850409 31.440263195304723 18.905992973335227 42 23.048313861537675 28.07662874343146 30.41426457833784 18.460792816693026 43 22.13258327706219 29.81473677797353 29.367952356533966 18.684727588430313 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 3.0 7 6.0 8 59.0 9 112.0 10 296.0 11 480.0 12 480.0 13 677.0 14 874.0 15 1050.5 16 1227.0 17 1315.5 18 1404.0 19 1404.0 20 1942.0 21 2480.0 22 3083.0 23 3686.0 24 4017.0 25 4348.0 26 4348.0 27 4125.5 28 3903.0 29 3704.5 30 3506.0 31 3650.5 32 3795.0 33 3795.0 34 5334.0 35 6873.0 36 6716.5 37 6560.0 38 7800.5 39 9041.0 40 9041.0 41 9459.5 42 9878.0 43 10738.5 44 11599.0 45 14273.0 46 16947.0 47 16947.0 48 16445.0 49 15943.0 50 17485.0 51 19027.0 52 18262.5 53 17498.0 54 17498.0 55 16283.0 56 15068.0 57 14674.0 58 14280.0 59 11029.0 60 7778.0 61 7778.0 62 6456.0 63 5134.0 64 4124.0 65 3114.0 66 2440.0 67 1766.0 68 1766.0 69 1626.0 70 1486.0 71 1292.5 72 1099.0 73 2734.0 74 4369.0 75 4369.0 76 3235.5 77 2102.0 78 1572.0 79 1042.0 80 695.5 81 349.0 82 349.0 83 267.5 84 186.0 85 95.0 86 4.0 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001523113243469652 2 0.002030817657959536 3 5.07704414489884E-4 4 5.07704414489884E-4 5 5.07704414489884E-4 6 5.07704414489884E-4 7 0.0 8 0.001015408828979768 9 0.11169497118777448 10 0.023354403066534666 11 0.03249308252735258 12 0.03249308252735258 13 0.03452390018531211 14 0.0253852207244942 15 0.00507704414489884 16 0.0 17 0.0 18 0.0 19 0.013708019191226866 20 0.0035539309014291877 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.00253852207244942 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.001015408828979768 38 0.0 39 0.0 40 0.002030817657959536 41 5.07704414489884E-4 42 0.48485771583783915 43 0.001015408828979768 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 196965.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.232020917421877 #Duplication Level Percentage of deduplicated Percentage of total 1 62.90338770388959 12.726626558017923 2 12.065244667503137 4.882085649734725 3 6.0326223337515685 3.661564237301043 4 3.6938519447929736 2.9893635925164372 5 2.481806775407779 2.5105983296524763 6 1.5508155583437893 1.8825679689284898 7 1.3726474278544543 1.9440002030817658 8 1.0489335006273526 1.697763562054172 9 0.7703889585947302 1.4027872972355495 >10 6.7151819322459225 30.88822887315005 >50 0.9209535759096612 12.08641129134618 >100 0.4090338770388959 15.392074734089814 >500 0.022584692597239647 2.951793465844186 >1k 0.012547051442910915 4.984134237047192 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCGGCACCGCGTGCGCGATCTTCGCCCGGCGCAGGCGCTGGA 3477 1.7652882491813267 No Hit ATCGGTGACTGTTTGCGTCGGTCCAGGCTCCGTGCGAGTGTAA 2181 1.107303328002437 No Hit ATCGGTACACTGAGCCGGCCAGTTGAGTTACAAAACATTTCTC 1625 0.8250196735460615 No Hit ATCGGTCGCGCGGTCCGACGATCCAATCATCGGACTGTAGAAC 1503 0.7630797349782956 No Hit ACCTGTACGTCAGACCTGGGCCATGGGAGGCTGGCGCTATGTT 1031 0.5234432513390704 No Hit ATCGGTACATTCCGCACCAAATCAACCTGATAAAGGTTGATGA 842 0.4274871170004823 No Hit ATCGGCACCGCGTGGCGCGATCTTCGCCCGGCGCAGGCGCTGG 696 0.3533622724849592 No Hit ATCGGTACATCACGGTGCCCAATCCGCCTGAGTTAATCCATTG 692 0.35133145482699973 No Hit ATCGGTACGTATGCATGAAACACTGTCTCTTCTATAGGATCAT 679 0.34473129743863123 No Hit ATCGGTACGCGCATCGTATCATCGGTACTTTTTTTTTTTTTTT 630 0.3198537811286269 No Hit ATTGGCACAGTCTGGTCCTTTGTAGGGACATGGATGAAGCTGG 619 0.3142690325692382 No Hit ATCGGTACCCCGCTCCGCCCCCCGGGCCGCGGTTTTCCGCGCG 569 0.288883811844744 No Hit ATCGGTACCATCAGTGGGCTCCTCTGACCCCCTGAGCATGGTA 546 0.27720661031147664 No Hit ATCGGTACAAGGTATCTATTCCCACTCTTTATATAAAGCGTTA 541 0.2746680882390272 No Hit ATCGGTACGCTTAGCGGTACTTTTTTTTTTTTTTTTTTTTTTT 494 0.25080598075800264 No Hit ATCGGTACGGAGAGACTGCAGCATTTCATAATGTTTGTCTAGC 465 0.23608255273779608 No Hit ATCGGTACGGACCGTCCGACGATCGAGGTATCGGTATACTGAT 460 0.23354403066534662 No Hit ATCGGATCTCGAGCAGTCCGACGATCTCGACGTAAAAGACAGT 439 0.22288223796105908 No Hit ATCGGTACCATCAGGTGGGCTCCTCTGACCCCCTGAGCATGGT 427 0.21678978498718046 No Hit ATCGGTACGATTCAATGGACTCGGGTCATGATAAGGGATTGGG 420 0.2132358540857513 No Hit ATCGGTACAGCAACAGTCCGACGATCCGTCGATCGGACTGTAG 414 0.21018962759881196 No Hit ACCTGTACGTCAGGACCTGGGCCATGGGAGGCTGGCGCTATGT 403 0.20460487903942326 No Hit ATCGGAATCTGCACAACACGGGAAACCTCACCCGGCCCGGACA 400 0.2030817657959536 No Hit ATCGGTACAGGGCGCCTCAGCTTCCTCAACTGTACCTCACAGG 376 0.19089685984819638 No Hit ATCGGTACAGGGCGGCCTCAGCTTCCTCAACTGTACCTCACAG 361 0.1832812936308481 No Hit ATCGGTACGCTTACGGTACTTTTTTTTTTTTTTTTTTTTTTTT 361 0.1832812936308481 No Hit ATCGGTACGCATCAAACACAGTGGCCTCCTACCGTGGCTATGT 353 0.17921965831492903 No Hit ATCGGTACCGATCAGTCCGACGATCTTGGCATCGGTCTGTAGA 353 0.17921965831492903 No Hit ATCGGTACACTGAGGCCGGCCAGTTGAGTTACAAAACATTTCT 341 0.17312720534105044 No Hit ATCGGCACCGCGTCGCGCGATCTTCGCCCGGCGCAGGCGCTGG 333 0.16906557002513137 No Hit ATCGGTACATCACGGGTGCCCAATCCGCCTGAGTTAATCCATT 332 0.16855786561064148 No Hit ATCGGTACGTTGCGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 316 0.16043459497880336 No Hit ATCGGTACTCGACGTCCGACGATCCCATCATCGGACTGTAGAA 310 0.15738836849186402 No Hit AACGGTAGGTCATGGACCATCCTGGCAATAGTCCTCAGTTGCA 302 0.15332673317594497 No Hit ATCGCTATGTCAGGTCCGACGATCGTCATACAGTGTGGTACCT 300 0.1523113243469652 No Hit ATCGGTACAGCTCCAGTCCGACGATCTGATCATCGGACTGTAG 300 0.1523113243469652 No Hit ATCAGTACGAGGCCGAACCATGCCGCATCTGCATGGTTCGTGT 293 0.148757393445536 No Hit ACCTGTACGTCAGCACCTGGGCCATGGGAGGCTGGCGCTATGT 283 0.14368034930063717 No Hit ATCGGAATCTGCACCAACACGGGAAACCTCACCCGGCCCGGAC 279 0.14164953164267763 No Hit AACGGTAGGTCATGACCATCCTGGCAATAGTCCTCAGTTGCAG 278 0.14114182722818774 No Hit ATCGGTACTCATAGGAGAGGAGAGATAACTACGTTCCTGAGGT 277 0.14063412281369786 No Hit ATCGGTACAGTTAGTCCGACGATCATTCGATCGGACTGTAGAA 276 0.14012641839920797 No Hit ATCGGTACGCTTATCGGTACTTTTTTTTTTTTTTTTTTTTTTT 268 0.1360647830832889 No Hit ATCGGTACTCACACTACAGTCCGACGATCACTTTATCGTCGGA 266 0.13504937425430913 No Hit ATCGGTACTTCCCACTGAAAATCACGGAAAATGAGAAATACAA 265 0.13454166983981927 No Hit ATCGGTACAGTGCTCCGACGATCCAATCATCGGACTGTAGAAC 262 0.13301855659634962 No Hit ATCGGTACCGTGACAGTCCGACGATCGTCGGACTGTAGAACTC 259 0.13149544335287996 Illumina DpnII expression Sequencing Primer (100% over 21bp) ATCGGTACTCTACACTTGCTGCCAAGTCAGAGGACATCAGTCC 254 0.12895692128043054 No Hit ATCGGTACTCCCGGCCAACCTGGGTAGGTGGATGAGGGGCCTC 252 0.12794151245145077 No Hit ATCGGTACAGCTGGCGGGGAAGGATCAACGCTTATACAGACCG 247 0.12540299037900132 No Hit ATCGGTACGCTTACCGGTACTTTTTTTTTTTTTTTTTTTTTTT 243 0.12337217272104181 No Hit ATCGGAATCTCGACCAACACGGGAAACCTCACCCGGCCCGGAC 240 0.12184905947757216 No Hit ATCGGTACACTGACGCCGGCCAGTTGAGTTACAAAACATTTCT 236 0.11981824181961263 No Hit ATCTGTATATGGCTGTGGAAAATCTGGAACTTTCCACATTTTT 234 0.11880283299063284 No Hit ATCAGTACGAGGCGCCGCATCTGCATGGTTCGTGTCCAGATAG 233 0.11829512857614297 No Hit ATCGGTACGGCTTTGAGTAACAAGAATTCCAATTCTCCAGGCA 225 0.1142334932602239 No Hit ATCGGTACGAAAAAAGAAGTGAAGCAACAAACAGCTTCACTTC 224 0.113725788845734 No Hit ATCGGTACAAGGTGATCTATTCCCACTCTTTATATAAAGCGTT 220 0.11169497118777448 No Hit ATCGGTACCCACAGGTGTGCTGGATACCCCAGCACACCCAAAA 219 0.11118726677328458 No Hit ATCGGTACGTTGGCAGTCCGACGATCTTCTGATCGTCGGACTG 219 0.11118726677328458 No Hit ATCAGTACGAGGCCGCCGCATCTGCATGGTTCGTGTCCAGATA 216 0.10966415352981493 No Hit ATCGGTACATGTATCCGCCCCCCGGGCCGCGGTTTTCCGCGCG 209 0.10611022262838575 No Hit ATAGGTTCAGCTAGGGAAACTTTGCCAGGGCACCAAGGACTTG 208 0.10560251821389588 No Hit ATCGGTGACTGTTCTGCGTCGGTCCAGGCTCCGTGCGAGTGTA 202 0.10255629172695657 No Hit ATCGGTACGGAAGGCAGAGTTTTGGATTTCAATTTACACATAT 198 0.10052547406899702 No Hit ATTGGCACAGTCTCCATGTCCTTTGTAGGGACATGGATGAAGC 197 0.10001776965450715 No Hit GTAGGTACAGTCTCCGACGATCAGTCCAAAAGAAGAGCTTGCA 197 0.10001776965450715 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 5.07704414489884E-4 0.0 0.0 15 0.0 0.0 0.003046226486939304 0.0 0.0 16 0.0 0.0 0.00507704414489884 0.0 0.0 17 0.0 0.0 0.01878506333612571 0.0 0.0 18 0.0 0.0 0.02944685604041327 0.0 0.0 19 0.0 0.0 0.058386007666336656 0.0 0.0 20 0.0 0.0 0.14977280227451578 0.0 0.0 21 0.0 0.0 0.24623664102759374 0.0 0.0 22 0.0 0.0 0.3391465488792425 0.0 0.0 23 0.0 0.0 0.5884294163937756 0.0 0.0 24 0.0 0.0 0.756987282004417 0.0 0.0 25 0.0 0.0 0.801157566065037 0.0 0.0 26 0.0 0.0 0.9595613433858807 0.0 0.0 27 0.0 0.0 1.2895692128043053 0.0 0.0 28 0.0 0.0 1.5820069555504785 0.0 0.0 29 0.0 0.0 1.8267204833346027 0.0 0.0 30 0.0 0.0 1.9856319650699363 0.0 0.0 31 0.0 0.0 2.4481506866702207 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGTT 85 0.0 37.16148 37 GAAATAT 20 0.0017993225 37.16148 37 ATAATAT 30 8.033259E-6 37.16148 36 GCTGCTA 35 5.4118027E-7 37.16148 37 AGTGTCT 25 1.1987741E-4 37.16148 36 CCCTGCC 50 1.7644197E-10 37.010418 8 CAGCGAT 25 1.2278755E-4 37.010418 8 TCTGACG 25 1.2278755E-4 37.010418 13 AGTCATG 20 0.001835693 37.010418 9 TGCTTCG 40 3.796231E-8 37.010418 9 CACGGCA 20 0.001835693 37.010418 8 CAACGCC 25 1.2278755E-4 37.010418 8 CGCGGCC 25 1.2278755E-4 37.010418 8 CATTGCG 25 1.2278755E-4 37.010418 13 CGTGACA 20 0.001835693 37.010418 9 ATTACGC 20 0.001835693 37.010418 9 TGGCGGA 20 0.001835693 37.010418 9 CTCCCGG 45 2.590241E-9 37.010418 8 CCGGACG 20 0.001835693 37.010418 9 CCGCTCT 20 0.001835693 37.010418 8 >>END_MODULE