FastQCFastQC Report
Thu 26 May 2016
SRR2099073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87343
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGCACCGCGTGCGCGATCTTCGCCCGGCGCAGGCGCTGGA14721.6853096412992454No Hit
ATCGGTGACTGTTTGCGTCGGTCCAGGCTCCGTGCGAGTGTAA9621.1014048063382298No Hit
ATCGGTACACTGAGCCGGCCAGTTGAGTTACAAAACATTTCTC7600.8701326952360234No Hit
ATCGGTCGCGCGGTCCGACGATCCAATCATCGGACTGTAGAAC6700.7670906655370207No Hit
ACCTGTACGTCAGACCTGGGCCATGGGAGGCTGGCGCTATGTT4630.5300939972293143No Hit
ATCGGTACATCACGGTGCCCAATCCGCCTGAGTTAATCCATTG3310.3789656870041102No Hit
ATCGGCACCGCGTGGCGCGATCTTCGCCCGGCGCAGGCGCTGG3110.3560674581821096No Hit
ATTGGCACAGTCTGGTCCTTTGTAGGGACATGGATGAAGCTGG2910.333169229360109No Hit
ATCGGTACGTATGCATGAAACACTGTCTCTTCTATAGGATCAT2860.3274446721546088No Hit
ATCGGTACATTCCGCACCAAATCAACCTGATAAAGGTTGATGA2710.3102710005381084No Hit
ATCGGTACCCCGCTCCGCCCCCCGGGCCGCGGTTTTCCGCGCG2630.3011117090093081No Hit
ATCGGTACGCTTAGCGGTACTTTTTTTTTTTTTTTTTTTTTTT2620.2999667975682081No Hit
ATCGGTACGCGCATCGTATCATCGGTACTTTTTTTTTTTTTTT2440.2793583916284076No Hit
ATCGGTACAAGGTATCTATTCCCACTCTTTATATAAAGCGTTA2430.2782134801873075No Hit
ATCGGATCTCGAGCAGTCCGACGATCTCGACGTAAAAGACAGT2050.23470684542550635No Hit
ATCGGTACCATCAGTGGGCTCCTCTGACCCCCTGAGCATGGTA1990.22783737677890617No Hit
ATCGGAATCTGCACAACACGGGAAACCTCACCCGGCCCGGACA1910.2186780852501059No Hit
ATCGGTACTCATAGGAGAGGAGAGATAACTACGTTCCTGAGGT1880.21524335092680583No Hit
ATCGGTACAGGGCGCCTCAGCTTCCTCAACTGTACCTCACAGG1860.21295352804460577No Hit
ATCGGTACGCTTACGGTACTTTTTTTTTTTTTTTTTTTTTTTT1770.20264932507470546No Hit
ATCGGTACGATTCAATGGACTCGGGTCATGATAAGGGATTGGG1710.1957798564281053No Hit
ATCGGTACGGACCGTCCGACGATCGAGGTATCGGTATACTGAT1680.1923451221048052No Hit
ATCGGTACAGGGCGGCCTCAGCTTCCTCAACTGTACCTCACAG1660.19005529922260514No Hit
ATCGGTACCATCAGGTGGGCTCCTCTGACCCCCTGAGCATGGT1620.185475653458205No Hit
ATCGCTATGTCAGGTCCGACGATCGTCATACAGTGTGGTACCT1590.18204091913490492No Hit
ATCGGTACCGATCAGTCCGACGATCTTGGCATCGGTCTGTAGA1590.18204091913490492No Hit
ATCGGTACTCCACGCAGAGGGGCCTATAGGTCCTTCACTGTTC1580.1808960076938049No Hit
ATCGGTACGTTGCGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1570.17975109625270486No Hit
ATCGGTACGGAGAGACTGCAGCATTTCATAATGTTTGTCTAGC1570.17975109625270486No Hit
AACGGTAGGTCATGGACCATCCTGGCAATAGTCCTCAGTTGCA1500.17173671616500463No Hit
ATCGGTACACTGAGGCCGGCCAGTTGAGTTACAAAACATTTCT1490.17059180472390462No Hit
ATCGGCACCGCGTCGCGCGATCTTCGCCCGGCGCAGGCGCTGG1470.16830198184170456No Hit
ATCGGTACGCATCAAACACAGTGGCCTCCTACCGTGGCTATGT1470.16830198184170456No Hit
ACCTGTACGTCAGGACCTGGGCCATGGGAGGCTGGCGCTATGT1470.16830198184170456No Hit
ATCGGTACATCACGGGTGCCCAATCCGCCTGAGTTAATCCATT1460.1671570704006045No Hit
AACGGTAGGTCATGACCATCCTGGCAATAGTCCTCAGTTGCAG1430.1637223360773044No Hit
ATCGGTCCCGCGCTCCGACGATCCAATCATCGGACTGTAGAAC1400.16028760175400433No Hit
ATCAGTACGAGGCCGAACCATGCCGCATCTGCATGGTTCGTGT1370.15685286743070423No Hit
ATCGGTACTCCTTGGAAAGACATAGAAAAGGCTTGCCAGTCCA1370.15685286743070423No Hit
ATCGGAATCTGCACCAACACGGGAAACCTCACCCGGCCCGGAC1360.1557079559896042No Hit
ATCGGTACAGTTAGTCCGACGATCATTCGATCGGACTGTAGAA1330.1522732216663041No Hit
ATCGGTACTCGACGTCCGACGATCCCATCATCGGACTGTAGAA1300.14883848734300403No Hit
ATCGGTACGCTTACCGGTACTTTTTTTTTTTTTTTTTTTTTTT1290.147693575901904No Hit
ATCGGTACGCTTATCGGTACTTTTTTTTTTTTTTTTTTTTTTT1290.147693575901904No Hit
ATCGGTACAGCAACAGTCCGACGATCCGTCGATCGGACTGTAG1240.14196901869640383No Hit
ATCGGTACTCACACTACAGTCCGACGATCACTTTATCGTCGGA1230.1408241072553038No Hit
ATCGGTACAGCTCCAGTCCGACGATCTGATCATCGGACTGTAG1160.1328097271676036No Hit
ATCGGTACACTGACGCCGGCCAGTTGAGTTACAAAACATTTCT1140.13051990428540353No Hit
AGCGGAACAGCTACTTCAGTCCTGATGCATTTAGCTGATCGTC1140.13051990428540353No Hit
ATCGGTACGAAAAAAGAAGTGAAGCAACAAACAGCTTCACTTC1140.13051990428540353No Hit
ATCGGTACAAGGCGCCAAGGTGGGTGGATCACGAGTTCAGGAG1130.1293749928443035No Hit
ATCGGTACTTCCCACTGAAAATCACGGAAAATGAGAAATACAA1100.1259402585210034No Hit
ATCGGTACCGTGACAGTCCGACGATCGTCGGACTGTAGAACTC1060.12136061275660327Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACCTGTACGTCAGCACCTGGGCCATGGGAGGCTGGCGCTATGT1060.12136061275660327No Hit
ATCTGTATATGGCTGTGGAAAATCTGGAACTTTCCACATTTTT1050.12021570131550326No Hit
ATCGGTACTCTACACTTGCTGCCAAGTCAGAGGACATCAGTCC1040.11907078987440321No Hit
ATCAGTACGAGGCCGCCGCATCTGCATGGTTCGTGTCCAGATA1040.11907078987440321No Hit
ATCGGAATCTCGACCAACACGGGAAACCTCACCCGGCCCGGAC1030.11792587843330318No Hit
ATCAGTACGAGGCGCCGCATCTGCATGGTTCGTGTCCAGATAG1020.11678096699220317No Hit
ATCGGTACCACAACTAGAGCCCAAGCCCTGGGCCCCACGCTTG1000.11449114411000308No Hit
ATCGGTACGGCTTTGAGTAACAAGAATTCCAATTCTCCAGGCA1000.11449114411000308No Hit
ATCGGTACGTTGGCAGTCCGACGATCTTCTGATCGTCGGACTG990.11334623266890306No Hit
CTGGGTACGTACATCGTACGATCGTCGGACTGTAGAACTCTGA970.11105640978670299Illumina DpnII expression Sequencing Primer (100% over 24bp)
ATCGGTACGGAAGGCAGAGTTTTGGATTTCAATTTACACATAT970.11105640978670299No Hit
ATCGGTACGCCTAGATGAAGAGCAGAAACTCCTTCTGCAGATG970.11105640978670299No Hit
ATCGGTACAGAGTAAAGAGCCCATCCTTTGCAAGTCATCCATG960.10991149834560297No Hit
ATCGGTACATGTATCCGCCCCCCGGGCCGCGGTTTTCCGCGCG950.10876658690450293No Hit
ATCGGAATTCATTCCAACACGGGAAACCTCACCCGGCCCGGAC950.10876658690450293No Hit
ATCGGAATTCATTCAACACGGGAAACCTCACCCGGCCCGGACA930.10647676402230288No Hit
ATCGGTACAGAGGCTTTTGTTTCTGTCTCTCCCATATACAGTC930.10647676402230288No Hit
ATAGGTCCTCGATTACCCCTTCTTCCATGACCCACCATCTGTC920.10533185258120284No Hit
ATCGGTACCCACAGGTGTGCTGGATACCCCAGCACACCCAAAA910.10418694114010282No Hit
AACGGAACGTCATGGACGATCGTCAATAGACTAGGTACCTAGA890.10189711825790275No Hit
ATCGGTACAAGGTGATCTATTCCCACTCTTTATATAAAGCGTT890.10189711825790275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG308.198049E-637.01719310
GTCATCT200.001828455237.0171939
GTTTGCG850.037.01719311
CTTCGAT200.001828455237.01719311
CGGGTGC308.198049E-637.01719313
TCTTGAC501.7462298E-1037.0171938
CGAACCA308.198049E-637.01719314
CGTATGT200.001828455237.0171938
CGTATGC355.532038E-737.0171938
TCCGCAC200.001828455237.01719311
CTTCCTA200.001828455237.01719310
GTCTGGT308.198049E-637.01719310
TGCTTCG308.198049E-637.0171939
CTCCGAC551.2732926E-1137.01719313
CGACGTC200.001828455237.01719310
ATAGGAG200.001828455237.01719311
GCGGTCC750.037.01719310
CGTGCGC1300.037.01719311
CAGTTAG308.198049E-637.0171938
GACCGTC403.748937E-837.01719310
Produced by FastQC (version 0.11.5)