##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099073_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87343 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.06030248560274 36.0 35.0 37.0 32.0 37.0 2 34.57687507871266 36.0 35.0 37.0 31.0 37.0 3 35.47511534982769 37.0 35.0 37.0 32.0 37.0 4 36.497109098611226 39.0 37.0 39.0 31.0 39.0 5 36.21012559678509 39.0 37.0 39.0 30.0 39.0 6 35.40480633822974 39.0 35.0 39.0 27.0 39.0 7 36.21372061871014 39.0 35.0 39.0 32.0 39.0 8 36.714035469356446 39.0 37.0 39.0 32.0 39.0 9 31.259768956871184 33.0 31.0 34.0 28.0 34.0 10 31.59719725679219 33.0 31.0 34.0 28.0 34.0 11 31.58635494544497 33.0 31.0 34.0 28.0 34.0 12 34.915139163985664 37.0 35.0 37.0 32.0 37.0 13 34.777005598616945 37.0 35.0 37.0 32.0 37.0 14 31.936045246900154 31.0 31.0 34.0 30.0 34.0 15 31.941426330673323 31.0 31.0 34.0 30.0 34.0 16 32.001877654763405 33.0 31.0 34.0 30.0 34.0 17 35.252876589995765 37.0 35.0 37.0 32.0 37.0 18 35.429147155467525 37.0 35.0 37.0 33.0 37.0 19 34.64291357063531 37.0 35.0 37.0 30.0 37.0 20 33.98455514465956 37.0 35.0 37.0 27.0 37.0 21 33.92548916341321 37.0 33.0 37.0 27.0 37.0 22 35.371775643154 39.0 34.0 39.0 27.0 39.0 23 35.54304294562816 39.0 35.0 39.0 27.0 39.0 24 35.76047307740746 39.0 35.0 39.0 27.0 39.0 25 35.80183872777441 39.0 35.0 39.0 27.0 39.0 26 35.70060565815234 39.0 35.0 39.0 27.0 39.0 27 37.12598605497865 40.0 36.0 41.0 30.0 41.0 28 37.25165153475378 40.0 36.0 41.0 30.0 41.0 29 37.339328852913226 40.0 36.0 41.0 30.0 41.0 30 37.428139633399354 40.0 36.0 41.0 31.0 41.0 31 37.563983375885876 40.0 36.0 41.0 32.0 41.0 32 37.82378668010029 40.0 36.0 41.0 32.0 41.0 33 37.94862782363784 40.0 36.0 41.0 33.0 41.0 34 38.04232737597747 40.0 36.0 41.0 33.0 41.0 35 38.06809933251663 40.0 37.0 41.0 34.0 41.0 36 38.143594792942764 40.0 37.0 41.0 34.0 41.0 37 38.185155078254695 40.0 37.0 41.0 34.0 41.0 38 38.147956905533356 40.0 37.0 41.0 34.0 41.0 39 38.15245640749688 40.0 37.0 41.0 35.0 41.0 40 38.118028920463004 40.0 37.0 41.0 34.0 41.0 41 38.03984291815028 40.0 37.0 41.0 34.0 41.0 42 37.982311118235 40.0 37.0 41.0 34.0 41.0 43 37.85848894588003 40.0 37.0 41.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7285974729989206 1101 2 0.9110875907870124 1101 3 0.7624821101785102 1101 4 1.6066272685676992 1101 5 1.0573107403537492 1101 6 1.361379758300039 1101 7 0.9169357316420559 1101 8 1.0628654373258541 1101 9 0.8670672982132821 1101 10 1.028142354314177 1101 11 1.0226928192939972 1101 12 0.9225146396188038 1101 13 1.1897657416351564 1101 14 -0.05319630277945109 1101 15 0.050905463514190785 1101 16 -0.1270660779960906 1101 17 -0.11937250926254706 1101 18 -0.40585300625331655 1101 19 -0.47994619430755137 1101 20 -0.10029477665274555 1101 21 -0.6224563545056228 1101 22 -1.5169487642100847 1101 23 -0.7612350301212487 1101 24 -1.1524839248269956 1101 25 -1.095545501043489 1101 26 -1.1552510359275985 1101 27 -1.338488819277579 1101 28 -0.7705602846759447 1101 29 -0.6684427674484041 1101 30 -0.46937443797730793 1101 31 -0.6038760950140869 1101 32 -0.49480862124759284 1101 33 -0.24423737307942162 1101 34 -0.12450584117149788 1101 35 -0.38557866333943736 1101 36 -0.49069820754403537 1101 37 -0.4875428381890785 1101 38 -0.07772723398135639 1101 39 0.10910839337716283 1101 40 0.2632486162278269 1101 41 0.35413335410503777 1101 42 0.31938085104985703 1101 43 0.4616695970493012 1102 1 0.4174115166813053 1102 2 0.28269404108299767 1102 3 0.1416411222217846 1102 4 0.4111792179451186 1102 5 0.6591682622970012 1102 6 0.5906102134949762 1102 7 0.8132411000845963 1102 8 0.15849719890984915 1102 9 -0.22213457972098638 1102 10 0.42567246249952007 1102 11 0.15220904709559235 1102 12 0.1652172319845917 1102 13 -0.08590276214112436 1102 14 -0.11994461876230389 1102 15 -0.30722884961093655 1102 16 -0.24933592898608836 1102 17 0.17374853584869498 1102 18 -0.43361377375688903 1102 19 -0.6890705053914417 1102 20 -0.8982127190899831 1102 21 0.008590797974470377 1102 22 -0.10400734759445385 1102 23 -0.49995721832292617 1102 24 -1.1035963967600395 1102 25 -1.0235920411537194 1102 26 -0.4075474229453562 1102 27 -0.5999707543663675 1102 28 -0.8039344426673694 1102 29 -0.9894266417084552 1102 30 -0.2647408392836965 1102 31 0.15791202501040402 1102 32 -0.018282799651352377 1102 33 0.0027518083861792775 1102 34 0.20770481406426455 1102 35 -0.18849762639312218 1102 36 0.5094038541012011 1102 37 0.646566163648032 1102 38 0.6890619066595889 1102 39 0.6639154968676735 1102 40 0.7443672118595899 1102 41 0.7569706678425305 1102 42 0.7120120002639894 1102 43 0.6221105033567156 1103 1 0.8398929702884459 1103 2 0.8187654312560966 1103 3 0.7245354346613126 1103 4 0.9220382653593759 1103 5 0.9251611662858252 1103 6 1.0944807650485728 1103 7 1.2319595174590745 1103 8 1.0551212178325429 1103 9 0.6698662804015783 1103 10 0.6423695918404562 1103 11 0.7140303566321968 1103 12 0.4354032334898079 1103 13 0.9245262239894032 1103 14 -0.1517691537585435 1103 15 -0.37087791960340866 1103 16 -0.6928366278356748 1103 17 -0.7326593772789209 1103 18 -0.47532971827898507 1103 19 -0.8446989324422987 1103 20 -0.7813435652355594 1103 21 -1.2069236628361892 1103 22 -1.5178338178664674 1103 23 -0.6232772914333395 1103 24 -0.7814804952440753 1103 25 -0.5087549268649312 1103 26 -1.1613910956687192 1103 27 -0.33483797294501727 1103 28 -0.9152665574937302 1103 29 -0.8324542731459346 1103 30 -0.18007252404877505 1103 31 -0.6825352387246753 1103 32 -0.6831037866420004 1103 33 -0.22039624021073934 1103 34 -0.7298825421339927 1103 35 -0.47507971434065155 1103 36 -0.27524295806953347 1103 37 -0.3488106775518389 1103 38 0.09939412900127564 1103 39 0.018501025305475594 1103 40 0.13303509703322902 1103 41 0.1338656253739785 1103 42 0.0917561138001659 1103 43 0.012283603023419687 1104 1 -0.123191476276439 1104 2 0.14999157629426207 1104 3 0.39450203771474435 1104 4 0.21509647146624644 1104 5 0.13419026933925693 1104 6 0.7178109177203282 1104 7 0.087338811352204 1104 8 0.20779297355773707 1104 9 0.0675881315466178 1104 10 0.39827369489388786 1104 11 0.15176890625052053 1104 12 0.5697066686043115 1104 13 -0.4089661424228126 1104 14 -0.18057402017075574 1104 15 -0.2722376524278367 1104 16 -0.15514578814101654 1104 17 -0.09638790781327344 1104 18 0.25707724736987103 1104 19 -0.060109237785809455 1104 20 0.37830576682551253 1104 21 -0.01715744146214604 1104 22 -0.09971597435501423 1104 23 0.16256478871932956 1104 24 0.27833582154981684 1104 25 0.6150523250434645 1104 26 0.41166472494196427 1104 27 0.13671498507024893 1104 28 0.1540321066284065 1104 29 -0.5633703036802871 1104 30 -0.5955066843541204 1104 31 -0.21664783414452415 1104 32 -0.34376695458092854 1104 33 0.05512856894956286 1104 34 0.07522241969806487 1104 35 -0.24076435174523425 1104 36 -0.08082501913823847 1104 37 0.3514517270283122 1104 38 0.11984975877226844 1104 39 0.0938230672902094 1104 40 0.24920876115536572 1104 41 0.3345454917861943 1104 42 0.4999741481513098 1104 43 0.5713842709623478 1105 1 0.19506191519642613 1105 2 0.7484654135035669 1105 3 0.3175882780248216 1105 4 0.43372534743523516 1105 5 0.913562846858639 1105 6 0.9099136308986218 1105 7 0.3016677842204203 1105 8 0.44035111309261765 1105 9 0.5066021819342161 1105 10 0.3206937530334244 1105 11 0.38602278221950925 1105 12 0.38729387790663594 1105 13 0.2543090513756354 1105 14 -0.387877799240524 1105 15 -0.16092466793171312 1105 16 -0.3707465633348157 1105 17 -0.7562110667279995 1105 18 -0.5463990123200517 1105 19 -0.7236266796462729 1105 20 -0.46901739208921356 1105 21 -0.5361739143303623 1105 22 -1.3032649084635395 1105 23 -1.0905238740713656 1105 24 -1.542945670698245 1105 25 -0.8299573648790144 1105 26 -1.0536332401743138 1105 27 -1.3614075924491331 1105 28 -1.3448777770925204 1105 29 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1106 22 -0.1806053974319397 1106 23 -0.8551034805114384 1106 24 -0.590053601527103 1106 25 -0.2373515211103836 1106 26 -0.19638815967341827 1106 27 -0.04417443800667087 1106 28 -0.713035201063903 1106 29 -0.36721645752643894 1106 30 -0.001035530507969895 1106 31 0.45498678124009473 1106 32 0.010319583880608718 1106 33 -0.3684291233581263 1106 34 -0.4964121956865526 1106 35 -0.1468941594375437 1106 36 -0.321690403753621 1106 37 -0.17282711912553594 1106 38 0.43174879723380855 1106 39 -0.09523943270978918 1106 40 -0.13396431576771306 1106 41 0.12024260717080892 1106 42 0.3924020327666966 1106 43 -0.21960130596072958 1107 1 0.259621023723561 1107 2 0.15780407629426207 1107 3 0.29293953771474435 1107 4 0.04322147146624644 1107 5 0.07950276933925693 1107 6 0.061560917720328234 1107 7 0.251401311352204 1107 8 0.47341797355773707 1107 9 0.5050881315466178 1107 10 0.5545236948938879 1107 11 0.47208140625052053 1107 12 0.8118941686043115 1107 13 0.7082213575771874 1107 14 0.27255097982924426 1107 15 0.1418248475721633 1107 16 0.16516671185898346 1107 17 0.35673709218672656 1107 18 0.11645224736987103 1107 19 -0.060109237785809455 1107 20 -1.3638817331744875 1107 21 -0.25934494146214604 1107 22 0.18153402564498577 1107 23 -0.36087271128067044 1107 24 0.29396082154981684 1107 25 -0.09588517495653548 1107 26 0.31791472494196427 1107 27 0.21483998507024893 1107 28 0.2868446066284065 1107 29 0.014754696319712934 1107 30 0.24043081564587965 1107 31 0.025539665855475846 1107 32 0.31248304541907146 1107 33 0.33637856894956286 1107 34 0.5283474196980649 1107 35 0.6811106482547657 1107 36 0.3488624808617615 1107 37 0.5467642270283122 1107 38 0.8776622587722684 1107 39 0.6641355672902094 1107 40 0.4445212611553657 1107 41 0.6470454917861943 1107 42 0.6874741481513098 1107 43 0.6729467709623478 1108 1 0.1376028939525682 1108 2 0.06304024041639877 1108 3 0.34285604534833425 1108 4 0.22738177680975724 1108 5 0.24577185330872453 1108 6 -0.37116808991326167 1108 7 -0.0733839939913068 1108 8 -0.05174901117509023 1108 9 0.1126143720046322 1108 10 -0.1438899501442812 1108 11 0.20257997495281188 1108 12 -0.22108531612851579 1108 13 -0.228145531735791 1108 14 -0.20520426826236005 1108 15 -0.2029389883057 1108 16 -0.26535571180513884 1108 17 -0.19830823224075544 1108 18 -0.29975719919501387 1108 19 -0.09660732938885985 1108 20 -0.7737099774492933 1108 21 -0.32906106741634744 1108 22 -0.5712689323702804 1108 23 -0.6614452303646416 1108 24 -1.0410224799769026 1108 25 -0.287380881063406 1108 26 -0.7339101796381868 1108 27 -0.7317845378305137 1108 28 -0.7320604506235 1108 29 -0.49657641055051727 1108 30 -0.29457634084266715 1108 31 -0.506962719640704 1108 32 0.04208705305266136 1108 33 -0.006655782195473137 1108 34 -0.05812681694315813 1108 35 -0.36057589754675945 1108 36 -0.046235324481749274 1108 37 -0.012932814956421623 1108 38 -0.37388831374681786 1108 39 0.0948369031680727 1108 40 -0.11887329991333928 1108 41 0.0727969230839065 1108 42 0.4276339763955832 1108 43 0.5237339847028082 1109 1 -0.18478304604388285 1109 2 0.17482200265084913 1109 3 0.15479758035040447 1109 4 -0.35697232698336734 1109 5 -0.2802356027537698 1109 6 0.514564793689317 1109 7 -0.4270143863222131 1109 8 -0.3580984993104792 1109 9 0.2120285385233629 1109 10 0.15790305535900373 1109 11 0.43253441012648963 1109 12 -0.11658209108561124 1109 13 -0.05576846800421009 1109 14 0.15475785967420208 1109 15 0.08713734757216329 1109 16 0.18739297154890622 1109 17 0.08875017358207771 1109 18 0.1357715303156084 1109 19 -0.018200323057129708 1109 20 0.4612151660503159 1109 21 0.7894074810184719 1109 22 0.5106885799085532 1109 23 0.47536759879685064 1109 24 0.7206202207746202 1109 25 0.022755813415557213 1109 26 0.543265984631887 1109 27 0.8478947331322644 1109 28 0.6783780368609627 1109 29 0.2602731071724236 1109 30 0.6691493233978179 1109 31 0.07526108058415559 1109 32 0.29588905317100966 1109 33 0.1401576387170067 1109 34 -0.11978727022441404 1109 35 -0.030674719962291874 1109 36 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0.6644074740974872 1110 30 0.5895627600903239 1110 31 0.2173799436332544 1110 32 0.3125987861598105 1110 33 -0.005751060680069031 1110 34 0.1708242715499182 1110 35 0.0311685186251367 1110 36 0.2295916475284301 1110 37 0.13628968999127267 1110 38 0.47106503655004417 1110 39 0.789945752475397 1110 40 0.18369950189610762 1110 41 -0.3395864526582528 1110 42 -0.21443557407091163 1110 43 -0.45749304385246603 1111 1 -0.3148807878706421 1111 2 -0.19036168457530067 1111 3 -0.012880208662068071 1111 4 -0.08177852853375356 1111 5 0.3036875519479523 1111 6 0.17297396119858632 1111 7 0.04012413743915744 1111 8 0.03387993007947898 1111 9 -0.36481675975772987 1111 10 -0.34142286307712766 1111 11 -0.18745210824223335 1111 12 -0.10647086762757141 1111 13 0.13904110395399982 1111 14 -0.016964418721482133 1111 15 -0.02880468141334447 1111 16 0.03960058867057725 1111 17 0.20671444725918775 1111 18 0.036175979253926016 1111 19 -0.037917208800301694 1111 20 0.5953573972602939 1111 21 -0.3470940356650445 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-0.5223753918301384 2216 39 -0.42415509536039053 2216 40 -0.7895713593265654 2216 41 -0.7879130925511575 2216 42 -0.8536591349812213 2216 43 -0.37882280735090745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 6.0 26 31.0 27 143.0 28 335.0 29 763.0 30 1749.0 31 3019.0 32 5437.0 33 8733.0 34 10972.0 35 10402.0 36 9012.0 37 13049.0 38 23692.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.6136725324296165 98.39826889390105 0.20035950219250542 0.7876990714768212 2 0.7510705044537564 1.9383572622564171 95.5450985780037 1.7654736552861168 3 0.8953207469402242 1.10941918642593 1.9726824130153535 96.02257765361848 4 96.14961874012504 1.5593872363811223 1.954386205949028 0.3366078175448238 5 96.50573028176271 1.401371603906438 0.8449446435318229 1.2479534707990336 6 1.1907215314510773 6.058940715806829 87.30622152000184 5.444116232740263 7 2.9115097947173787 92.26497830392819 0.9205087986444248 3.903003102710005 8 2.664269930502284 3.0455341706644075 4.893463550909654 89.39673234792366 9 23.494927751952186 27.709588037280454 18.88525040646683 29.910233804300535 10 33.70199903826338 12.910169219848411 27.181653729019256 26.206178012868953 11 23.8555955025304 19.223018617325792 25.04980649888937 31.87157938125444 12 34.39013499124103 17.411465668258167 25.429647694615237 22.768751645885573 13 19.462572416477755 26.51759749032539 23.390808545716837 30.629021547480022 14 39.03846814553534 22.646846619865094 17.78536171967155 20.529323514928024 15 26.276319254416595 24.67455147067242 24.84171236876152 24.207416906149458 16 21.388090631189677 33.89166847944311 22.014357189471394 22.705883699895814 17 30.918333466906333 23.71340576806384 22.200977754370697 23.167283010659126 18 21.165977811616273 38.61099343965744 19.403959103763324 20.819069644962962 19 29.420319728825984 29.025239338555263 15.981860656864091 25.572580275754657 20 24.65420903178529 25.016030044884126 23.313410277548776 27.016350645781806 21 21.356033110838876 26.793217544622923 25.002576050742476 26.848173293795725 22 19.845894920027938 24.165645787298352 28.871231810219477 27.117227482454233 23 20.37484400581615 23.927504207549546 30.614931935014827 25.08271985161948 24 21.11789153109007 27.00044651546203 27.073720847692428 24.80794110575547 25 22.697869319808113 26.027271790527003 27.342775036350936 23.932083853313944 26 23.174152479305725 24.79992672566777 29.391021604478894 22.63489919054761 27 22.016647012353594 25.56358265688149 29.041823614943386 23.377946715821533 28 20.603826294036157 24.505684485305064 30.550816894313225 24.339672326345557 29 22.079617141614097 23.235977697125126 32.083853313946165 22.60055184731461 30 22.1838040827542 26.010098118910502 30.808421968560733 20.99767582977457 31 20.698853943647457 24.254948879704155 30.685916444363027 24.360280732285357 32 23.237122608566228 23.73630399688584 29.790595697422805 23.235977697125126 33 22.613145873166708 23.822172354968345 33.765728220922114 19.798953550942837 34 21.613638185086383 27.42177392578684 33.1497658656103 17.81482202351648 35 23.266890306034828 25.47885921024009 30.991607799136737 20.262642684588346 36 23.98818451392785 24.44042453316236 31.47247060439875 20.098920348511044 37 21.806482637020412 25.11535246905806 30.021410334207303 23.056754559714225 38 22.253643680661302 24.73581168496617 31.90295730625236 21.107587328120168 39 21.073239984887167 25.273920062283185 34.07943395578352 19.573405997046127 40 22.50769988206872 25.110202773102507 33.38256678001809 18.99953056481068 41 23.760619175196354 24.513979528749054 33.03221817682215 18.693183119232444 42 22.69099985116151 26.866491876853328 32.19147498940957 18.251033282575595 43 22.443067974948764 28.459715368498184 30.68318430061483 18.41403235593822 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.5 7 5.0 8 54.0 9 103.0 10 255.5 11 408.0 12 408.0 13 530.5 14 653.0 15 766.5 16 880.0 17 839.5 18 799.0 19 799.0 20 959.5 21 1120.0 22 1407.5 23 1695.0 24 1866.5 25 2038.0 26 2038.0 27 1895.0 28 1752.0 29 1656.0 30 1560.0 31 1585.0 32 1610.0 33 1610.0 34 2290.0 35 2970.0 36 2924.5 37 2879.0 38 3432.5 39 3986.0 40 3986.0 41 4121.0 42 4256.0 43 4600.0 44 4944.0 45 6320.0 46 7696.0 47 7696.0 48 7272.5 49 6849.0 50 7581.5 51 8314.0 52 7925.0 53 7536.0 54 7536.0 55 7133.5 56 6731.0 57 6546.5 58 6362.0 59 4905.5 60 3449.0 61 3449.0 62 2878.0 63 2307.0 64 1766.0 65 1225.0 66 984.0 67 743.0 68 743.0 69 694.0 70 645.0 71 538.0 72 431.0 73 1134.5 74 1838.0 75 1838.0 76 1365.0 77 892.0 78 663.0 79 434.0 80 296.0 81 158.0 82 158.0 83 116.0 84 74.0 85 37.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0011449114411000308 3 0.0 4 0.0011449114411000308 5 0.0 6 0.0011449114411000308 7 0.0 8 0.0022898228822000617 9 0.005724557205500154 10 0.0011449114411000308 11 0.005724557205500154 12 0.004579645764400123 13 0.0011449114411000308 14 0.027477874586400743 15 0.0022898228822000617 16 0.0 17 0.0 18 0.0 19 0.021753317380900587 20 0.008014380087700217 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0022898228822000617 38 0.0 39 0.0 40 0.004579645764400123 41 0.0011449114411000308 42 0.0 43 0.0022898228822000617 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 87343.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.502089463380006 #Duplication Level Percentage of deduplicated Percentage of total 1 58.49699806655134 13.163046838327055 2 13.356059835148063 6.0107850657751625 3 6.283708151012517 4.241896889275615 4 4.039890098707642 3.636238736933698 5 2.5185712831993485 2.8336558167225765 6 1.9385366846443473 2.6172675543546706 7 1.5569349750686885 2.452400306836266 8 1.20077337946474 2.1615928007968583 9 1.0379566500457922 2.102057405859657 >10 8.746311183474102 37.64468818336902 >50 0.5240663478172383 8.034988493640018 >100 0.2798412536888165 10.677444099698889 >500 0.015264068383026354 2.738628167111274 >1k 0.005088022794342118 1.6853096412992454 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCGGCACCGCGTGCGCGATCTTCGCCCGGCGCAGGCGCTGGA 1472 1.6853096412992454 No Hit ATCGGTGACTGTTTGCGTCGGTCCAGGCTCCGTGCGAGTGTAA 962 1.1014048063382298 No Hit ATCGGTACACTGAGCCGGCCAGTTGAGTTACAAAACATTTCTC 760 0.8701326952360234 No Hit ATCGGTCGCGCGGTCCGACGATCCAATCATCGGACTGTAGAAC 670 0.7670906655370207 No Hit ACCTGTACGTCAGACCTGGGCCATGGGAGGCTGGCGCTATGTT 463 0.5300939972293143 No Hit ATCGGTACATCACGGTGCCCAATCCGCCTGAGTTAATCCATTG 331 0.3789656870041102 No Hit ATCGGCACCGCGTGGCGCGATCTTCGCCCGGCGCAGGCGCTGG 311 0.3560674581821096 No Hit ATTGGCACAGTCTGGTCCTTTGTAGGGACATGGATGAAGCTGG 291 0.333169229360109 No Hit ATCGGTACGTATGCATGAAACACTGTCTCTTCTATAGGATCAT 286 0.3274446721546088 No Hit ATCGGTACATTCCGCACCAAATCAACCTGATAAAGGTTGATGA 271 0.3102710005381084 No Hit ATCGGTACCCCGCTCCGCCCCCCGGGCCGCGGTTTTCCGCGCG 263 0.3011117090093081 No Hit ATCGGTACGCTTAGCGGTACTTTTTTTTTTTTTTTTTTTTTTT 262 0.2999667975682081 No Hit ATCGGTACGCGCATCGTATCATCGGTACTTTTTTTTTTTTTTT 244 0.2793583916284076 No Hit ATCGGTACAAGGTATCTATTCCCACTCTTTATATAAAGCGTTA 243 0.2782134801873075 No Hit ATCGGATCTCGAGCAGTCCGACGATCTCGACGTAAAAGACAGT 205 0.23470684542550635 No Hit ATCGGTACCATCAGTGGGCTCCTCTGACCCCCTGAGCATGGTA 199 0.22783737677890617 No Hit ATCGGAATCTGCACAACACGGGAAACCTCACCCGGCCCGGACA 191 0.2186780852501059 No Hit ATCGGTACTCATAGGAGAGGAGAGATAACTACGTTCCTGAGGT 188 0.21524335092680583 No Hit ATCGGTACAGGGCGCCTCAGCTTCCTCAACTGTACCTCACAGG 186 0.21295352804460577 No Hit ATCGGTACGCTTACGGTACTTTTTTTTTTTTTTTTTTTTTTTT 177 0.20264932507470546 No Hit ATCGGTACGATTCAATGGACTCGGGTCATGATAAGGGATTGGG 171 0.1957798564281053 No Hit ATCGGTACGGACCGTCCGACGATCGAGGTATCGGTATACTGAT 168 0.1923451221048052 No Hit ATCGGTACAGGGCGGCCTCAGCTTCCTCAACTGTACCTCACAG 166 0.19005529922260514 No Hit ATCGGTACCATCAGGTGGGCTCCTCTGACCCCCTGAGCATGGT 162 0.185475653458205 No Hit ATCGCTATGTCAGGTCCGACGATCGTCATACAGTGTGGTACCT 159 0.18204091913490492 No Hit ATCGGTACCGATCAGTCCGACGATCTTGGCATCGGTCTGTAGA 159 0.18204091913490492 No Hit ATCGGTACTCCACGCAGAGGGGCCTATAGGTCCTTCACTGTTC 158 0.1808960076938049 No Hit ATCGGTACGTTGCGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 157 0.17975109625270486 No Hit ATCGGTACGGAGAGACTGCAGCATTTCATAATGTTTGTCTAGC 157 0.17975109625270486 No Hit AACGGTAGGTCATGGACCATCCTGGCAATAGTCCTCAGTTGCA 150 0.17173671616500463 No Hit ATCGGTACACTGAGGCCGGCCAGTTGAGTTACAAAACATTTCT 149 0.17059180472390462 No Hit ATCGGCACCGCGTCGCGCGATCTTCGCCCGGCGCAGGCGCTGG 147 0.16830198184170456 No Hit ATCGGTACGCATCAAACACAGTGGCCTCCTACCGTGGCTATGT 147 0.16830198184170456 No Hit ACCTGTACGTCAGGACCTGGGCCATGGGAGGCTGGCGCTATGT 147 0.16830198184170456 No Hit ATCGGTACATCACGGGTGCCCAATCCGCCTGAGTTAATCCATT 146 0.1671570704006045 No Hit AACGGTAGGTCATGACCATCCTGGCAATAGTCCTCAGTTGCAG 143 0.1637223360773044 No Hit ATCGGTCCCGCGCTCCGACGATCCAATCATCGGACTGTAGAAC 140 0.16028760175400433 No Hit ATCAGTACGAGGCCGAACCATGCCGCATCTGCATGGTTCGTGT 137 0.15685286743070423 No Hit ATCGGTACTCCTTGGAAAGACATAGAAAAGGCTTGCCAGTCCA 137 0.15685286743070423 No Hit ATCGGAATCTGCACCAACACGGGAAACCTCACCCGGCCCGGAC 136 0.1557079559896042 No Hit ATCGGTACAGTTAGTCCGACGATCATTCGATCGGACTGTAGAA 133 0.1522732216663041 No Hit ATCGGTACTCGACGTCCGACGATCCCATCATCGGACTGTAGAA 130 0.14883848734300403 No Hit ATCGGTACGCTTACCGGTACTTTTTTTTTTTTTTTTTTTTTTT 129 0.147693575901904 No Hit ATCGGTACGCTTATCGGTACTTTTTTTTTTTTTTTTTTTTTTT 129 0.147693575901904 No Hit ATCGGTACAGCAACAGTCCGACGATCCGTCGATCGGACTGTAG 124 0.14196901869640383 No Hit ATCGGTACTCACACTACAGTCCGACGATCACTTTATCGTCGGA 123 0.1408241072553038 No Hit ATCGGTACAGCTCCAGTCCGACGATCTGATCATCGGACTGTAG 116 0.1328097271676036 No Hit ATCGGTACACTGACGCCGGCCAGTTGAGTTACAAAACATTTCT 114 0.13051990428540353 No Hit AGCGGAACAGCTACTTCAGTCCTGATGCATTTAGCTGATCGTC 114 0.13051990428540353 No Hit ATCGGTACGAAAAAAGAAGTGAAGCAACAAACAGCTTCACTTC 114 0.13051990428540353 No Hit ATCGGTACAAGGCGCCAAGGTGGGTGGATCACGAGTTCAGGAG 113 0.1293749928443035 No Hit ATCGGTACTTCCCACTGAAAATCACGGAAAATGAGAAATACAA 110 0.1259402585210034 No Hit ATCGGTACCGTGACAGTCCGACGATCGTCGGACTGTAGAACTC 106 0.12136061275660327 Illumina DpnII expression Sequencing Primer (100% over 21bp) ACCTGTACGTCAGCACCTGGGCCATGGGAGGCTGGCGCTATGT 106 0.12136061275660327 No Hit ATCTGTATATGGCTGTGGAAAATCTGGAACTTTCCACATTTTT 105 0.12021570131550326 No Hit ATCGGTACTCTACACTTGCTGCCAAGTCAGAGGACATCAGTCC 104 0.11907078987440321 No Hit ATCAGTACGAGGCCGCCGCATCTGCATGGTTCGTGTCCAGATA 104 0.11907078987440321 No Hit ATCGGAATCTCGACCAACACGGGAAACCTCACCCGGCCCGGAC 103 0.11792587843330318 No Hit ATCAGTACGAGGCGCCGCATCTGCATGGTTCGTGTCCAGATAG 102 0.11678096699220317 No Hit ATCGGTACCACAACTAGAGCCCAAGCCCTGGGCCCCACGCTTG 100 0.11449114411000308 No Hit ATCGGTACGGCTTTGAGTAACAAGAATTCCAATTCTCCAGGCA 100 0.11449114411000308 No Hit ATCGGTACGTTGGCAGTCCGACGATCTTCTGATCGTCGGACTG 99 0.11334623266890306 No Hit CTGGGTACGTACATCGTACGATCGTCGGACTGTAGAACTCTGA 97 0.11105640978670299 Illumina DpnII expression Sequencing Primer (100% over 24bp) ATCGGTACGGAAGGCAGAGTTTTGGATTTCAATTTACACATAT 97 0.11105640978670299 No Hit ATCGGTACGCCTAGATGAAGAGCAGAAACTCCTTCTGCAGATG 97 0.11105640978670299 No Hit ATCGGTACAGAGTAAAGAGCCCATCCTTTGCAAGTCATCCATG 96 0.10991149834560297 No Hit ATCGGTACATGTATCCGCCCCCCGGGCCGCGGTTTTCCGCGCG 95 0.10876658690450293 No Hit ATCGGAATTCATTCCAACACGGGAAACCTCACCCGGCCCGGAC 95 0.10876658690450293 No Hit ATCGGAATTCATTCAACACGGGAAACCTCACCCGGCCCGGACA 93 0.10647676402230288 No Hit ATCGGTACAGAGGCTTTTGTTTCTGTCTCTCCCATATACAGTC 93 0.10647676402230288 No Hit ATAGGTCCTCGATTACCCCTTCTTCCATGACCCACCATCTGTC 92 0.10533185258120284 No Hit ATCGGTACCCACAGGTGTGCTGGATACCCCAGCACACCCAAAA 91 0.10418694114010282 No Hit AACGGAACGTCATGGACGATCGTCAATAGACTAGGTACCTAGA 89 0.10189711825790275 No Hit ATCGGTACAAGGTGATCTATTCCCACTCTTTATATAAAGCGTT 89 0.10189711825790275 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0011449114411000308 0.0 0.0 16 0.0 0.0 0.0011449114411000308 0.0 0.0 17 0.0 0.0 0.004579645764400123 0.0 0.0 18 0.0 0.0 0.008014380087700217 0.0 0.0 19 0.0 0.0 0.035492254674100956 0.0 0.0 20 0.0 0.0 0.1786061848116048 0.0 0.0 21 0.0 0.0 0.36064710394650973 0.0 0.0 22 0.0 0.0 0.4385010819413118 0.0 0.0 23 0.0 0.0 0.666338458720218 0.0 0.0 24 0.0 0.0 0.8334955291208225 0.0 0.0 25 0.0 0.0 0.8815818096470238 0.0 0.0 26 0.0 0.0 1.0601879944586285 0.0 0.0 27 0.0 0.0 1.3269523602349358 0.0 0.0 28 0.0 0.0 1.624629334920944 0.0 0.0 29 0.0 0.0 1.9085673723137515 0.0 0.0 30 0.0 0.0 2.071144796949956 0.0 0.0 31 0.0 0.0 2.514225524655668 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGGG 30 8.198049E-6 37.017193 10 GTCATCT 20 0.0018284552 37.017193 9 GTTTGCG 85 0.0 37.017193 11 CTTCGAT 20 0.0018284552 37.017193 11 CGGGTGC 30 8.198049E-6 37.017193 13 TCTTGAC 50 1.7462298E-10 37.017193 8 CGAACCA 30 8.198049E-6 37.017193 14 CGTATGT 20 0.0018284552 37.017193 8 CGTATGC 35 5.532038E-7 37.017193 8 TCCGCAC 20 0.0018284552 37.017193 11 CTTCCTA 20 0.0018284552 37.017193 10 GTCTGGT 30 8.198049E-6 37.017193 10 TGCTTCG 30 8.198049E-6 37.017193 9 CTCCGAC 55 1.2732926E-11 37.017193 13 CGACGTC 20 0.0018284552 37.017193 10 ATAGGAG 20 0.0018284552 37.017193 11 GCGGTCC 75 0.0 37.017193 10 CGTGCGC 130 0.0 37.017193 11 CAGTTAG 30 8.198049E-6 37.017193 8 GACCGTC 40 3.748937E-8 37.017193 10 >>END_MODULE