FastQCFastQC Report
Thu 26 May 2016
SRR2099072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209003
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAGTCCTCCAGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT31681.5157677162528769No Hit
AGCTAGTCTCCTACCAAGCTGCCCGTTCTCATCTCTCTCAAGG23631.1306057807782663No Hit
AGCTAGTCCGGCGCAAGAATCTTCTATCCTGTCCTGTGCCTTA22531.0779749572972637No Hit
AGCTAATTAAGATAGACTCCCAAAGTGCTGGGATTATAGGTAT20550.9832394750314589No Hit
AGCTAGTGGACGTGCAGCCAGGCCCGGCCCCGGGGCGATGGCA18120.8669732013416075No Hit
AGCTAGTGACAGTAGCGGCTCTACGAATCGCCGAAGGAGCGTC17300.8277393147466783No Hit
AGCTAGTCCAGCGGTTGAGGACACACATCACAAATAAGTTTCT14550.6961622560441716No Hit
AGCTGATCTACGAGATCAGTGCATCCACAGTCACGTGGTCAGT11110.5315713171581269No Hit
AGCTAGTCACCGTTGTCTGGAGCCCTCGGAACTCCAGTGTAAT9940.47559125945560593No Hit
AGCTAGTAAGGCTCCTCAGCTTCCCAAAGTGCTGGAGTTCGCT9670.46267278460117794No Hit
AGCTAGTCGTTAAGCTAGCAGAGAGGGAAAAGAACTTGCATGT9450.4521466199049774No Hit
AGCTAGTCTCAACGGAGCCGGCAGAATGTCCGTGGAGCTGAGC8480.40573580283536603No Hit
AGCTAGTCTTGAGGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT8470.4052573408037205No Hit
AGCTAGTCCACGATACAGTCCGACGATCCTTGTAGCTAGTTAG7320.3502342071644905No Hit
AGCTAGTCCACCGACAGGGAGCCGGCCTGACCAGGCTTGTTCT6970.3334880360568987No Hit
AGCTAGTCTAGTCCGATCAAACGAGCTAGTCTTTTTTGATCGT6700.3205695612024708No Hit
AGCTAGTCATTACGAGGCAGCAAGATTGCTTGAGCCCAGGAGT6570.3143495547910795No Hit
AGCTAGTCAGTAGACAGTCCGACGATCACGCAAGCTAGTCTGA6460.3090864724429793No Hit
AGCTAGTGGACGTGGCAGCCAGGCCCGGCCCCGGGGCGATGGC6210.2971249216518423No Hit
AGCTAGTCCATAAACTGGAGTCACCCACGGAAGTTCGGCCAGG5750.2751156681961503No Hit
AGCCAGTTCAGTCGAAGGCCAGGACTTCTTCCCGCTGACCGTC5710.2732018200695684No Hit
AGCTTGTCACTCCGTTACGCCATCGAGGAGCCGGCGAGCCTGA5650.27033104787969553No Hit
AACTAGTCTGGCAACGGTTTTGGCCAGAGCCATAGACATCACT5440.26028334521514046No Hit
AGCTAGCCGGGGCAGCTATTAACAAATATGCATGGAAGACTAT5360.2564556489619766No Hit
AACTCGTCCCAGCCCCCATTCCGGTGGCCGTGGCCTATCCCTG5320.25454180083539474No Hit
AGCTAGTCTCCGCCGATCAAACGAGCTAGTCTTTTTTGATCGT5000.23923101582273937No Hit
AGCCAGTTCAGTCGGAAGGCCAGGACTTCTTCCCGCTGACCGT4440.21243714205059255No Hit
AGTTATTCTCGATATACCAAACGACCATAAGATGGGAATGTCC4390.21004483189236517No Hit
AGATAATCAGCTTCCGACGATCAGCTGATTTGGAAAGTCCTGA4360.20860944579742877No Hit
AGCTTGTCACTCCTTTACGCCATCGAGGAGCCGGCGAGCCTGA4270.20430328751261945No Hit
TGCTGGTCAGCTAGAAGGCGGCAGCTCTGGCAAAGCAGCTGGA4200.20095405329110105No Hit
AGCTAGTCGCTCGCCTGACAGAGCTGCTGGCCAAGATCACCCA3980.19042788859490054No Hit
AGCTAGTCCAAAAACGACTCACTATAGTGAGTCGTATTACCGG3830.18325095812021838No Hit
AGCTAGTCACGCGTAGTCTTTTTTTTTTTTTTTTTTTTTTTTT3640.17416017951895427No Hit
AGCGAGTTAGCTACCTGCCTCTTGTAGCCACTGGGGACCTATG3600.17224633139237236No Hit
AGCTAGTCAGACGCAGTCCGACGATCCCGTTAGCTAGCACGAT3550.16985402123414498No Hit
AGCTAGTCTCTATTAGTTAACACATTTCAAATGCATTTACTCT3340.1598063185695899No Hit
AGCTTGTCACTCCTTACGCCATCGAGGAGCCGGCGAGCCTGAA3280.15693554637971702No Hit
AGCTAGTCTAAACGCAACTGGAGAAATCAAGTAGGAAGTAAAA3110.14880169184174388No Hit
AGCTTGTCACTCCATTACGCCATCGAGGAGCCGGCGAGCCTGA3030.14497399558858007No Hit
AGCTAGTCTGGACGATCCTGACACCATGCAAGCCCTAGAAAAG2990.14306014746199813No Hit
AGCGAGTTAGCTATCTGCCTCTTGTAGCCACTGGGGACCTATG2940.14066783730377078No Hit
AGCTAGTCCCTCGGTTGAGCATCCCGGGCCCTACTGTAGTGGT2860.13684014105060693No Hit
AGCTAGTCACCGTGTGTCTGGAGCCCTCGGAACTCCAGTGTAA2840.13588321698731598No Hit
AGCTAGTCGCTCGCCCTGACAGAGCTGCTGGCCAAGATCACCC2790.13349090682908857No Hit
AGCTAGTCTCTGCGGAAAAGGGTCTGTACCAGATGCCCTCTCC2770.13253398276579761No Hit
AGCTAGTCACACTAGTCCGACGATCTAAACAGCTAGTCTTTTT2720.13014167260757023No Hit
AGCTTCTCTCGAGAGCTCCTCATTAAAATGCACATAATTCAGC2700.12918474854427928No Hit
AGCTAGTCCGGGCAATAAAGCTAGTCTTTTTTGATCGTCGGAC2660.12727090041769737No Hit
AGCTAGTCATCGTCGTGGGACGAGCACTCGTTATTCATCCGCG2630.1258355143227609No Hit
AGCTAGTCCGCTGCTACAGTCCGACGATCCACCTAGCTTGTCC2590.123921666196179No Hit
AGATAGTAAGCTTCTTATTCTTCTAACTACCAGTCCAAAACTA2560.12248628010124256No Hit
AGCTGATCTACGAGGATCAGTGCATCCACAGTCACGTGGTCAG2560.12248628010124256No Hit
ATCTAGTGAGCTAGCTCCCAGGACAGAGGCCCCACTCTTCACA2460.11770165978478779No Hit
AGCTAGTCAGAATCTTATTCTTCTAACTACCAGTCCAAAACTA2460.11770165978478779No Hit
AGCTAGTGACAGTCGAATCGCCGAAGGAGCGTCCGATGGACGC2410.11530934962656039No Hit
AGCTAGTCAACGTGTGGAGGAAGAGCCACCTGCAAGATGGACG2280.10908934321516915No Hit
AGCTAGTAAGGCTTCCTCAGCTTCCCAAAGTGCTGGAGTTCGC2280.10908934321516915No Hit
AGCTAATTAAGATAGTGCTGGGATTATAGGTATGAGCCACTGT2250.10765395712023272No Hit
AGCTAGTAAGGCTGGCCTCAGCTTCCCAAAGTGCTGGAGTTCG2230.10669703305694178No Hit
AGCTAGTCCAGCGGGTTGAGGACACACATCACAAATAAGTTTC2190.10478318493035985No Hit
AGTTTGTCCATGTCGACGATCCATGTAAAGGGCATCAGTGACG2180.10430472289871438No Hit
AGCTAGCTTAAAAGCAGTGGCTCATATCTGTAATCCCAGCACT2170.1038262608670689No Hit
AGCTAGTCGTTAAGGCTAGCAGAGAGGGAAAAGAACTTGCATG2120.10143395070884151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAG200.001803463637.14515737
AGTATGC251.2021318E-437.14515736
TCAGGGT251.2209661E-437.0472229
CAACTCG251.2209661E-437.0472229
ACGCGTT251.2209661E-437.0472229
TGGACGG200.001827007337.047229
ATTACGA650.037.047229
CTCCCGT200.001827007337.047229
AGTGGTC200.001827007337.047229
CCTCGGT308.207277E-637.047229
AATGCCG200.001827007337.047229
ACCGTTG1250.037.047229
ACTCCGC200.001827007337.047229
ACTCCAT452.566594E-937.047229
GGGGCGC308.207277E-637.047229
GACGTGG950.037.0472159
CGTCGCT251.2226903E-437.0383458
CGTCGCG850.037.0383458
CAATGCA251.2226903E-437.0383458
CGATGCA251.2226903E-437.0383458
Produced by FastQC (version 0.11.5)