##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099072_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 209003 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.25191504428166 35.0 35.0 37.0 31.0 37.0 2 34.270048755281024 35.0 35.0 37.0 30.0 37.0 3 34.868446864399075 37.0 35.0 37.0 31.0 37.0 4 36.319325559920195 39.0 37.0 39.0 31.0 39.0 5 34.92739817131812 37.0 34.0 39.0 29.0 39.0 6 35.601761697200516 37.0 35.0 39.0 30.0 39.0 7 35.371219551872464 39.0 35.0 39.0 27.0 39.0 8 36.13080195021124 39.0 35.0 39.0 30.0 39.0 9 30.739046807940557 31.0 31.0 34.0 27.0 34.0 10 31.117342813261054 31.0 31.0 34.0 27.0 34.0 11 31.087104012861058 31.0 31.0 34.0 27.0 34.0 12 34.67803811428544 37.0 35.0 37.0 31.0 37.0 13 34.596642153461914 37.0 35.0 37.0 30.0 37.0 14 31.59629287617881 31.0 31.0 34.0 30.0 34.0 15 31.628364186160006 31.0 31.0 34.0 30.0 34.0 16 31.708544853423156 31.0 31.0 34.0 30.0 34.0 17 35.22305421453281 35.0 35.0 37.0 33.0 37.0 18 35.30045023277178 35.0 35.0 37.0 33.0 37.0 19 34.77483098328732 35.0 35.0 37.0 31.0 37.0 20 34.431917245207 35.0 35.0 37.0 30.0 37.0 21 34.36280818935613 35.0 35.0 37.0 30.0 37.0 22 35.79096472299441 38.0 35.0 39.0 30.0 39.0 23 35.92639340105166 38.0 35.0 39.0 30.0 39.0 24 36.089199676559666 38.0 35.0 39.0 30.0 39.0 25 36.20481524188648 38.0 35.0 39.0 30.0 39.0 26 36.190427888594904 38.0 35.0 39.0 30.0 39.0 27 37.48677291713516 39.0 36.0 41.0 32.0 41.0 28 37.68833461720645 40.0 37.0 41.0 32.0 41.0 29 37.907814720362865 40.0 37.0 41.0 32.0 41.0 30 38.04130084257164 40.0 37.0 41.0 33.0 41.0 31 38.213111773515216 40.0 37.0 41.0 33.0 41.0 32 38.39756367133486 40.0 38.0 41.0 34.0 41.0 33 38.46789758998675 40.0 38.0 41.0 34.0 41.0 34 38.550834198552174 40.0 38.0 41.0 34.0 41.0 35 38.60673770232963 40.0 38.0 41.0 35.0 41.0 36 38.53709755362363 40.0 38.0 41.0 35.0 41.0 37 38.47595489059966 40.0 38.0 41.0 35.0 41.0 38 38.402721492036 40.0 38.0 41.0 34.0 41.0 39 38.35031076108955 40.0 38.0 41.0 34.0 41.0 40 38.27904862609628 40.0 37.0 41.0 34.0 41.0 41 38.183021296345025 40.0 37.0 41.0 34.0 41.0 42 37.95684750936589 40.0 37.0 41.0 34.0 41.0 43 37.89917369607135 40.0 37.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.616793052651353 1101 2 0.8839943670406498 1101 3 0.7423990052745566 1101 4 0.8590313771574785 1101 5 0.9532518015105822 1101 6 0.9439707255624441 1101 7 1.4598951474590578 1101 8 1.0700585007623076 1101 9 0.7615071546120724 1101 10 0.617423192485294 1101 11 0.5941915746055066 1101 12 0.734501369761027 1101 13 0.7185676325984929 1101 14 -0.03627588042808938 1101 15 0.11193199112343066 1101 16 0.06057477694125879 1101 17 -0.03193527436074106 1101 18 -0.11030311309611562 1101 19 -0.2598231267828979 1101 20 -0.5207564927105821 1101 21 -0.04944141796963919 1101 22 -0.19015237443236543 1101 23 -0.36987265688849646 1101 24 -0.24012454598701538 1101 25 -0.2158097590915986 1101 26 -0.46655214880402696 1101 27 -0.36171803978613326 1101 28 -0.32700752774100295 1101 29 -0.2364839554192102 1101 30 -0.47949929279574377 1101 31 -0.36017102959644376 1101 32 -0.03448729719647048 1101 33 -0.29775991465780294 1101 34 -0.062019525885965265 1101 35 0.034179690841980914 1101 36 0.19212932366781388 1101 37 0.2717148702281307 1101 38 0.36404181825076876 1101 39 0.2394733325157432 1101 40 0.17544525198405836 1101 41 0.4464073226484331 1101 42 0.3999013963101561 1101 43 0.5420185533313955 1102 1 0.6090129130121298 1102 2 0.7196956298430166 1102 3 0.7523384277294198 1102 4 0.7794151749746732 1102 5 0.7444399111719022 1102 6 0.7020722370604133 1102 7 0.9093032351074584 1102 8 0.687235301494951 1102 9 0.6331768599581906 1102 10 0.8416021188932383 1102 11 0.5727331765076897 1102 12 0.6776794643829831 1102 13 0.5581925727351091 1102 14 0.3053193002543111 1102 15 0.30997267301990306 1102 16 0.2359639939701843 1102 17 0.3778411516481128 1102 18 0.12193321494683573 1102 19 0.21836139527624 1102 20 -0.1582742416792513 1102 21 0.1375255878528634 1102 22 0.061198227562236696 1102 23 0.1901797708343409 1102 24 0.288504855547167 1102 25 0.08736363047830764 1102 26 -0.16358880620061456 1102 27 -0.06879494218151194 1102 28 -0.1279082091333663 1102 29 -0.2724187904483273 1102 30 -0.12007532559668022 1102 31 -0.16078604711435673 1102 32 -0.24050136194134808 1102 33 -0.2985244856158715 1102 34 -0.06833773863833414 1102 35 0.09378261805650112 1102 36 0.31098229919314235 1102 37 0.21007868837786958 1102 38 0.18846775989131714 1102 39 0.2927076357059377 1102 40 0.24472714341497692 1102 41 0.22358109870475573 1102 42 0.4039006905523479 1102 43 0.24073558646000492 1103 1 0.6177679198898289 1103 2 0.684035414082274 1103 3 0.4368294348147401 1103 4 0.4546667083985554 1103 5 0.3403254039870873 1103 6 0.5020480137212289 1103 7 0.34674213605995874 1103 8 0.4045662355642108 1103 9 0.29550351168713007 1103 10 0.5528339622028398 1103 11 0.46837384057323206 1103 12 0.30950201112104736 1103 13 0.5241414787918117 1103 14 0.11926675291066147 1103 15 0.023860432447538216 1103 16 -0.03765592453558142 1103 17 0.1235047413739565 1103 18 -0.12323293120640955 1103 19 -0.0757185936502367 1103 20 -0.08061248600623117 1103 21 0.11027433126714214 1103 22 0.04585101194856378 1103 23 -0.024932132341511704 1103 24 -0.33419068303282273 1103 25 -0.42657776634324307 1103 26 -0.341059370517911 1103 27 -0.2810606030028566 1103 28 -0.4331568876636993 1103 29 -0.39765345404670427 1103 30 -0.48630626053105885 1103 31 -0.05906532491280103 1103 32 -0.13139252128765833 1103 33 -0.3130584891282595 1103 34 0.09697790012246799 1103 35 0.14132957240416033 1103 36 0.27200002548671165 1103 37 0.3314981760542466 1103 38 0.2570544145616651 1103 39 0.39570008321482675 1103 40 0.5149384920493887 1103 41 0.4447055807146043 1103 42 0.6326295708284917 1103 43 0.4890507667076065 1104 1 0.5325114552861834 1104 2 0.28261108173806093 1104 3 0.26295067262737604 1104 4 0.2318350000475533 1104 5 0.5088713980523707 1104 6 0.23390897175429615 1104 7 0.19315163160892013 1104 8 0.11429069508096035 1104 9 0.39440134975410857 1104 10 0.1683659672897413 1104 11 0.2542492114931143 1104 12 0.28555118449960304 1104 13 0.5793004444552281 1104 14 0.024794518913203945 1104 15 0.10280066135809562 1104 16 0.028092273853566496 1104 17 0.05830762395132183 1104 18 -0.06885395706482456 1104 19 0.11585410664650198 1104 20 0.306390481352814 1104 21 -0.10614788153489485 1104 22 0.12796207595874165 1104 23 0.22277452302093081 1104 24 0.10704712959964269 1104 25 -0.37578505756833636 1104 26 -0.11565877704610017 1104 27 -0.29415937366839273 1104 28 -0.18977409834619152 1104 29 -0.24507185167281875 1104 30 -0.12439019440134302 1104 31 -0.13314756314934328 1104 32 -0.20592410246612758 1104 33 -0.007912240717914187 1104 34 -0.028804210941544284 1104 35 -0.13105703208926656 1104 36 -0.010941899057591797 1104 37 -0.0715539646833534 1104 38 -0.05333982028361106 1104 39 -0.14536816604333325 1104 40 -0.09119122393195767 1104 41 0.06661316867560174 1104 42 0.4369619150421471 1104 43 0.24703296255329832 1105 1 0.08853772786417835 1105 2 0.18812832311736827 1105 3 -0.12427428632172166 1105 4 0.19186784077005115 1105 5 0.16921622563857852 1105 6 0.3304442755309793 1105 7 0.44039301091925864 1105 8 0.14140071150131917 1105 9 -0.02152640065640199 1105 10 0.08660898863621114 1105 11 0.1871227747115043 1105 12 0.0667498708707015 1105 13 0.34716579749299115 1105 14 0.1317074909985898 1105 15 0.0724722541331353 1105 16 0.10140918682565214 1105 17 -0.017964954045396553 1105 18 -0.0533038749630208 1105 19 -0.06587003128453262 1105 20 0.017080136525230216 1105 21 -0.1795633166744679 1105 22 0.08411971142672314 1105 23 0.09701097622289723 1105 24 0.27760542188207893 1105 25 0.09454334965661815 1105 26 -0.020371420724259792 1105 27 0.11208036360582696 1105 28 -0.008969500645044093 1105 29 -0.15219828845442152 1105 30 0.17978057735563624 1105 31 0.01383109038103214 1105 32 0.18581645582615636 1105 33 -0.01955427684270461 1105 34 0.03436491877931047 1105 35 0.24219419943782583 1105 36 0.11255563788822087 1105 37 0.13173010756623427 1105 38 0.15802306969997204 1105 39 0.4188847075198865 1105 40 0.14544260201220993 1105 41 0.1485836120253552 1105 42 0.31845616791570563 1105 43 0.29082606600157845 1106 1 -0.05119510842038011 1106 2 -0.27198351285653644 1106 3 0.14279623247293927 1106 4 0.15639098460354006 1106 5 -0.3046421154611423 1106 6 -0.06863929079396769 1106 7 0.2515300099872988 1106 8 0.07012081091107092 1106 9 -0.0909268355740771 1106 10 -0.008622449698673762 1106 11 -0.3225075452636439 1106 12 -0.47004727109885636 1106 13 -0.16170341654863307 1106 14 0.016531970650653705 1106 15 -0.020056481499047152 1106 16 0.043922389683682894 1106 17 0.16487133051502667 1106 18 0.15516148695061815 1106 19 0.0034216742140671386 1106 20 -0.14820411324177485 1106 21 -0.11564595103295972 1106 22 0.11869566669233222 1106 23 -0.2403142800678708 1106 24 -0.14306870051618148 1106 25 -0.46698196876524634 1106 26 -0.34701012839745005 1106 27 -0.2177114972205132 1106 28 -0.3335578821299734 1106 29 -0.32128806788902864 1106 30 -0.09605042606157355 1106 31 -0.031757601759380805 1106 32 -0.13094340748543232 1106 33 -0.1551709279765987 1106 34 -0.04185440399173501 1106 35 -0.25514969618193106 1106 36 -0.14275657087225824 1106 37 0.1110715179421291 1106 38 0.08303083608704753 1106 39 -0.13888167955684594 1106 40 -0.022233694974431728 1106 41 -0.015162893100459485 1106 42 0.1029078609880898 1106 43 0.1351410706614118 1107 1 0.26957221062279757 1107 2 0.6364041851863362 1107 3 0.38721996655183943 1107 4 0.7091092200803999 1107 5 0.7266874900063982 1107 6 0.8189500226574111 1107 7 0.4087838155169621 1107 8 0.6385271482829253 1107 9 0.09054256486084 1107 10 0.11534462082012098 1107 11 0.27620323448162054 1107 12 0.3052556179971404 1107 13 0.3557864871481655 1107 14 0.26676496226295754 1107 15 0.24201248401819342 1107 16 -0.004912652254805749 1107 17 0.2492764252649522 1107 18 0.2685352054967538 1107 19 0.3306816928533962 1107 20 0.20098818250224326 1107 21 0.16239070631404218 1107 22 -0.07967339202155443 1107 23 -0.43459821917939934 1107 24 -0.46090032524436 1107 25 -0.18131871930889787 1107 26 0.06583547582746263 1107 27 0.022999903835717816 1107 28 0.3214902694698978 1107 29 0.19262929775247528 1107 30 0.14817138195333968 1107 31 -0.08099649582586466 1107 32 -0.2883214752083276 1107 33 -0.022427840061098436 1107 34 -0.04896841455402523 1107 35 -0.19171384653918722 1107 36 -0.013881143720972489 1107 37 0.33287953285358896 1107 38 0.1407816903896233 1107 39 0.06830999487620915 1107 40 0.39544260201220993 1107 41 0.20318131317478105 1107 42 0.26960559320306743 1107 43 0.26783756025444916 1108 1 -0.23859518455285667 1108 2 0.28880826483665345 1108 3 0.21614986779839995 1108 4 0.49175451715017005 1108 5 0.2412657642495546 1108 6 0.5269069596818596 1108 7 0.6055459978060966 1108 8 0.47179572526204083 1108 9 0.1503369634361995 1108 10 0.4039394079336063 1108 11 0.3905872396621284 1108 12 0.041486798181693985 1108 13 -0.0025104207359234465 1108 14 -0.04003445492985591 1108 15 0.08980267343052972 1108 16 0.03151279699240206 1108 17 0.1621305615770794 1108 18 0.012755700882863152 1108 19 -0.006399414480263488 1108 20 0.0858271010711249 1108 21 -0.25862273062946173 1108 22 -0.35755100251208205 1108 23 -0.30563593975573156 1108 24 0.1327211738652423 1108 25 -0.18194199921823895 1108 26 -0.043546100989757974 1108 27 -0.3531130960023958 1108 28 -0.4047036758109783 1108 29 -0.014931006602395769 1108 30 -0.2613720857494286 1108 31 -0.2778558126463224 1108 32 -0.23638687912608702 1108 33 -0.03712753246841771 1108 34 -0.2807961626518036 1108 35 0.031679386421799904 1108 36 0.010345827300561439 1108 37 -0.051433240337281916 1108 38 -0.03752493094759046 1108 39 0.046462819872161276 1108 40 -0.19505440300640942 1108 41 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0.28209279902523576 1109 35 0.25462403103365716 1109 36 0.1545564398128434 1109 37 0.18492444063225832 1109 38 0.1346336016433014 1109 39 0.05544578744503781 1109 40 -0.19861647310139574 1109 41 0.05666300256264378 1109 42 0.2749851708560982 1109 43 0.5659864509253936 1110 1 0.18623887728946187 1110 2 -0.1538256998711347 1110 3 0.030898127471381542 1110 4 0.13439657640223857 1110 5 -0.2761860732119956 1110 6 -0.04024537964143349 1110 7 0.1616573787353559 1110 8 -0.14882917355615177 1110 9 0.0014621050907237532 1110 10 -0.21511514929482445 1110 11 0.05206530344713656 1110 12 -0.39014668085343374 1110 13 -0.026397420897808388 1110 14 -0.24760285382899738 1110 15 -0.13155073437261322 1110 16 -0.28939541087549614 1110 17 -0.3110684023212542 1110 18 -0.11652226576761393 1110 19 -0.376214858870739 1110 20 -0.3995865301414341 1110 21 -0.4295633166744679 1110 22 -0.19748948397558053 1110 23 -0.5926441961908964 1110 24 -0.23388883099148927 1110 25 -0.4571807882744139 1110 26 -0.5088771678506987 1110 27 0.022999903835717816 1110 28 -0.0808085811048116 1110 29 -0.045876449373963624 1110 30 -0.09608149160987978 1110 31 -0.07237580617069028 1110 32 0.047885421343394796 1110 33 0.03216986108832742 1110 34 0.06310055096322031 1110 35 -0.28079430630930347 1110 36 -0.35583516670947546 1110 37 -0.44298253611192706 1110 38 -0.17243670041496983 1110 39 -0.15870149937666866 1110 40 -0.05570682327514476 1110 41 -0.21635891671027707 1110 42 -0.043612797601532804 1110 43 0.043699629219965175 1111 1 -0.3389171161423903 1111 2 -1.3288174896905147 1111 3 -1.2741921845154778 1111 4 -1.3453078570952997 1111 5 -1.171128601947629 1111 6 -1.0718053139599917 1111 7 -1.249705511248223 1111 8 -0.9028521620618974 1111 9 -0.6141700788173203 1111 10 -0.43163403271026013 1111 11 -0.7657507885068853 1111 12 -0.4401631012146865 1111 13 -0.5264138412590569 1111 14 -0.30091976680108345 1111 15 -0.2286279100704789 1111 16 -0.16047915471786212 1111 17 -0.09026380462010763 1111 18 -0.06599681420767922 1111 19 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0.4416772885021203 2216 40 0.5640685163277794 2216 41 0.47421057127300514 2216 42 0.6365073695875978 2216 43 0.5815784170987541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 27.0 26 107.0 27 547.0 28 1425.0 29 2595.0 30 4157.0 31 6577.0 32 10588.0 33 15345.0 34 21416.0 35 27578.0 36 32248.0 37 45631.0 38 40761.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.6043148944721695 97.7942267113882 1.0387708914482026 0.5626875026914262 2 96.6842105263158 1.735885167464115 0.8564593301435407 0.723444976076555 3 1.0349183261404196 1.3023798815322343 1.8018966325681094 95.86080515975924 4 1.210514731916441 1.0377891120659133 96.31965244351728 1.432043712500359 5 2.141616547289247 93.36653891608174 2.939220386505328 1.5526241501236837 6 93.44554862848858 4.580795490973814 1.1707965914364866 0.8028592891011134 7 1.6894494337401855 1.838729587613575 92.69340631474189 3.7784146639043463 8 4.180861244019138 2.4272727272727272 5.493301435406699 87.89856459330143 9 21.08200019158923 30.91771242456174 21.232876712328768 26.76741067152026 10 25.849321165193505 26.450390264211983 19.397399514741988 28.302889055852525 11 26.19525106374832 22.603634692793314 18.759781937577475 32.44133230588089 12 26.926114363115094 23.59439240706262 17.42010520219594 32.05938802762635 13 24.132220328923438 24.451475177576533 22.897800156994887 28.518504336505142 14 35.94031165052261 24.65446609747693 17.209215513610783 22.196006738389677 15 27.44285529721944 24.92355983865025 21.927201213472607 25.706383650657692 16 18.480382775119615 36.18516746411483 26.580382775119617 18.754066985645935 17 28.88871876824145 30.083922641888595 21.333767140984296 19.693591448885655 18 23.842583732057417 34.32057416267942 18.69043062200957 23.146411483253587 19 26.706609177525635 27.096622911751616 19.415505797566123 26.78126211315662 20 23.220172731405057 26.142252206990594 23.86420727768607 26.773367783918278 21 23.849418429400533 26.746984493045556 23.185791591508256 26.217805486045652 22 20.935106194647922 25.978574470222917 26.238857815438056 26.84746151969111 23 22.30494299124893 28.60868025817811 24.78241939110922 24.30395735946374 24 21.52696372779338 29.177093151772944 23.337464055539872 25.958479064893808 25 24.20108802265996 25.59006330052679 26.63311052951393 23.575738147299322 26 20.632847190323076 28.916896340529778 28.097726228755164 22.352530240391978 27 22.178150552862878 28.077108941019986 30.594776151538493 19.149964354578643 28 21.433185169590868 27.87328411553901 27.517308363994776 23.176222350875346 29 22.559484792084326 25.645564896197666 27.76563015841878 24.029320153299235 30 22.08724276685024 25.930249805026722 29.53928890972857 22.443218518394474 31 22.312226677256678 25.770566788834557 27.149500961713287 24.76770557219548 32 23.922144658210648 26.03024836964063 28.735951158595807 21.311655813552914 33 23.669995167533482 27.875676425697236 28.00342578814658 20.4509026186227 34 23.831715334229653 27.213963435931543 29.489528858437435 19.46479237140137 35 24.70179568518811 25.113276969966652 29.581676642695488 20.60325070214975 36 23.430285689679096 27.607259225944127 29.87182002172218 19.090635062654606 37 23.75226675725721 27.465681653979207 30.323590065024238 18.45846152373935 38 25.61494332617235 27.425922115950492 29.28618249498811 17.67295206288905 39 22.77670655445137 25.377626158476197 30.753625546044795 21.09204174102764 40 21.349588750077753 25.24151064369345 29.82483002148356 23.584070584745234 41 22.617441315227897 26.127044277935674 32.31179245734409 18.94372194949234 42 24.99062707285889 25.722196052796015 29.383405592993856 19.90377128135124 43 22.009464069549907 26.299395696671308 31.63477686709633 20.056363366682454 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 14.0 9 27.0 10 92.5 11 158.0 12 158.0 13 225.5 14 293.0 15 358.5 16 424.0 17 706.5 18 989.0 19 989.0 20 1216.5 21 1444.0 22 2405.5 23 3367.0 24 4693.5 25 6020.0 26 6020.0 27 4838.0 28 3656.0 29 3930.0 30 4204.0 31 4431.5 32 4659.0 33 4659.0 34 5040.0 35 5421.0 36 7696.5 37 9972.0 38 10372.0 39 10772.0 40 10772.0 41 12584.0 42 14396.0 43 16235.5 44 18075.0 45 16599.5 46 15124.0 47 15124.0 48 15983.0 49 16842.0 50 18014.5 51 19187.0 52 19975.0 53 20763.0 54 20763.0 55 18041.0 56 15319.0 57 12850.0 58 10381.0 59 10388.0 60 10395.0 61 10395.0 62 8076.0 63 5757.0 64 4310.0 65 2863.0 66 2589.5 67 2316.0 68 2316.0 69 1696.0 70 1076.0 71 912.5 72 749.0 73 1616.0 74 2483.0 75 2483.0 76 1893.0 77 1303.0 78 818.5 79 334.0 80 216.5 81 99.0 82 99.0 83 97.0 84 95.0 85 67.0 86 39.0 87 19.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002870772189872873 2 0.0014353860949364364 3 4.7846203164547875E-4 4 4.7846203164547875E-4 5 9.569240632909575E-4 6 0.0 7 0.0 8 0.0014353860949364364 9 0.10669703305694178 10 0.02009540532911011 11 0.03444926627847447 12 0.03444926627847447 13 0.037798500499992824 14 0.024401563613919416 15 0.00765539250632766 16 0.0014353860949364364 17 4.7846203164547875E-4 18 0.0014353860949364364 19 0.017224633139237236 20 0.00382769625316383 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.005263082348100266 27 0.0 28 0.0 29 0.0 30 0.0 31 4.7846203164547875E-4 32 0.0 33 0.0 34 0.0 35 9.569240632909575E-4 36 0.0 37 0.001913848126581915 38 0.0 39 0.0 40 0.002870772189872873 41 0.002392310158227394 42 0.4578881642847232 43 9.569240632909575E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 209003.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.30018707865437 #Duplication Level Percentage of deduplicated Percentage of total 1 67.90327142453097 13.784491131706245 2 10.224380126331669 4.1511365865561745 3 5.199396624870369 3.1664617254297784 4 2.981521636655039 2.4210178801261226 5 1.8148392570943714 1.8420788218350932 6 1.4259451305741493 1.736817174873088 7 1.0535495427547845 1.4971076970187032 8 0.8390685396436316 1.3626598661263236 9 0.7282926369378712 1.3306029100060763 >10 6.512208918638635 31.26223068568394 >50 0.9474875082492694 12.464892848428013 >100 0.311115301216178 11.63428276149146 >500 0.04006787970208353 5.716185892068535 >1k 0.018855472800980485 7.63003401865045 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGCTAGTCCTCCAGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT 3168 1.5157677162528769 No Hit AGCTAGTCTCCTACCAAGCTGCCCGTTCTCATCTCTCTCAAGG 2363 1.1306057807782663 No Hit AGCTAGTCCGGCGCAAGAATCTTCTATCCTGTCCTGTGCCTTA 2253 1.0779749572972637 No Hit AGCTAATTAAGATAGACTCCCAAAGTGCTGGGATTATAGGTAT 2055 0.9832394750314589 No Hit AGCTAGTGGACGTGCAGCCAGGCCCGGCCCCGGGGCGATGGCA 1812 0.8669732013416075 No Hit AGCTAGTGACAGTAGCGGCTCTACGAATCGCCGAAGGAGCGTC 1730 0.8277393147466783 No Hit AGCTAGTCCAGCGGTTGAGGACACACATCACAAATAAGTTTCT 1455 0.6961622560441716 No Hit AGCTGATCTACGAGATCAGTGCATCCACAGTCACGTGGTCAGT 1111 0.5315713171581269 No Hit AGCTAGTCACCGTTGTCTGGAGCCCTCGGAACTCCAGTGTAAT 994 0.47559125945560593 No Hit AGCTAGTAAGGCTCCTCAGCTTCCCAAAGTGCTGGAGTTCGCT 967 0.46267278460117794 No Hit AGCTAGTCGTTAAGCTAGCAGAGAGGGAAAAGAACTTGCATGT 945 0.4521466199049774 No Hit AGCTAGTCTCAACGGAGCCGGCAGAATGTCCGTGGAGCTGAGC 848 0.40573580283536603 No Hit AGCTAGTCTTGAGGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT 847 0.4052573408037205 No Hit AGCTAGTCCACGATACAGTCCGACGATCCTTGTAGCTAGTTAG 732 0.3502342071644905 No Hit AGCTAGTCCACCGACAGGGAGCCGGCCTGACCAGGCTTGTTCT 697 0.3334880360568987 No Hit AGCTAGTCTAGTCCGATCAAACGAGCTAGTCTTTTTTGATCGT 670 0.3205695612024708 No Hit AGCTAGTCATTACGAGGCAGCAAGATTGCTTGAGCCCAGGAGT 657 0.3143495547910795 No Hit AGCTAGTCAGTAGACAGTCCGACGATCACGCAAGCTAGTCTGA 646 0.3090864724429793 No Hit AGCTAGTGGACGTGGCAGCCAGGCCCGGCCCCGGGGCGATGGC 621 0.2971249216518423 No Hit AGCTAGTCCATAAACTGGAGTCACCCACGGAAGTTCGGCCAGG 575 0.2751156681961503 No Hit AGCCAGTTCAGTCGAAGGCCAGGACTTCTTCCCGCTGACCGTC 571 0.2732018200695684 No Hit AGCTTGTCACTCCGTTACGCCATCGAGGAGCCGGCGAGCCTGA 565 0.27033104787969553 No Hit AACTAGTCTGGCAACGGTTTTGGCCAGAGCCATAGACATCACT 544 0.26028334521514046 No Hit AGCTAGCCGGGGCAGCTATTAACAAATATGCATGGAAGACTAT 536 0.2564556489619766 No Hit AACTCGTCCCAGCCCCCATTCCGGTGGCCGTGGCCTATCCCTG 532 0.25454180083539474 No Hit AGCTAGTCTCCGCCGATCAAACGAGCTAGTCTTTTTTGATCGT 500 0.23923101582273937 No Hit AGCCAGTTCAGTCGGAAGGCCAGGACTTCTTCCCGCTGACCGT 444 0.21243714205059255 No Hit AGTTATTCTCGATATACCAAACGACCATAAGATGGGAATGTCC 439 0.21004483189236517 No Hit AGATAATCAGCTTCCGACGATCAGCTGATTTGGAAAGTCCTGA 436 0.20860944579742877 No Hit AGCTTGTCACTCCTTTACGCCATCGAGGAGCCGGCGAGCCTGA 427 0.20430328751261945 No Hit TGCTGGTCAGCTAGAAGGCGGCAGCTCTGGCAAAGCAGCTGGA 420 0.20095405329110105 No Hit AGCTAGTCGCTCGCCTGACAGAGCTGCTGGCCAAGATCACCCA 398 0.19042788859490054 No Hit AGCTAGTCCAAAAACGACTCACTATAGTGAGTCGTATTACCGG 383 0.18325095812021838 No Hit AGCTAGTCACGCGTAGTCTTTTTTTTTTTTTTTTTTTTTTTTT 364 0.17416017951895427 No Hit AGCGAGTTAGCTACCTGCCTCTTGTAGCCACTGGGGACCTATG 360 0.17224633139237236 No Hit AGCTAGTCAGACGCAGTCCGACGATCCCGTTAGCTAGCACGAT 355 0.16985402123414498 No Hit AGCTAGTCTCTATTAGTTAACACATTTCAAATGCATTTACTCT 334 0.1598063185695899 No Hit AGCTTGTCACTCCTTACGCCATCGAGGAGCCGGCGAGCCTGAA 328 0.15693554637971702 No Hit AGCTAGTCTAAACGCAACTGGAGAAATCAAGTAGGAAGTAAAA 311 0.14880169184174388 No Hit AGCTTGTCACTCCATTACGCCATCGAGGAGCCGGCGAGCCTGA 303 0.14497399558858007 No Hit AGCTAGTCTGGACGATCCTGACACCATGCAAGCCCTAGAAAAG 299 0.14306014746199813 No Hit AGCGAGTTAGCTATCTGCCTCTTGTAGCCACTGGGGACCTATG 294 0.14066783730377078 No Hit AGCTAGTCCCTCGGTTGAGCATCCCGGGCCCTACTGTAGTGGT 286 0.13684014105060693 No Hit AGCTAGTCACCGTGTGTCTGGAGCCCTCGGAACTCCAGTGTAA 284 0.13588321698731598 No Hit AGCTAGTCGCTCGCCCTGACAGAGCTGCTGGCCAAGATCACCC 279 0.13349090682908857 No Hit AGCTAGTCTCTGCGGAAAAGGGTCTGTACCAGATGCCCTCTCC 277 0.13253398276579761 No Hit AGCTAGTCACACTAGTCCGACGATCTAAACAGCTAGTCTTTTT 272 0.13014167260757023 No Hit AGCTTCTCTCGAGAGCTCCTCATTAAAATGCACATAATTCAGC 270 0.12918474854427928 No Hit AGCTAGTCCGGGCAATAAAGCTAGTCTTTTTTGATCGTCGGAC 266 0.12727090041769737 No Hit AGCTAGTCATCGTCGTGGGACGAGCACTCGTTATTCATCCGCG 263 0.1258355143227609 No Hit AGCTAGTCCGCTGCTACAGTCCGACGATCCACCTAGCTTGTCC 259 0.123921666196179 No Hit AGATAGTAAGCTTCTTATTCTTCTAACTACCAGTCCAAAACTA 256 0.12248628010124256 No Hit AGCTGATCTACGAGGATCAGTGCATCCACAGTCACGTGGTCAG 256 0.12248628010124256 No Hit ATCTAGTGAGCTAGCTCCCAGGACAGAGGCCCCACTCTTCACA 246 0.11770165978478779 No Hit AGCTAGTCAGAATCTTATTCTTCTAACTACCAGTCCAAAACTA 246 0.11770165978478779 No Hit AGCTAGTGACAGTCGAATCGCCGAAGGAGCGTCCGATGGACGC 241 0.11530934962656039 No Hit AGCTAGTCAACGTGTGGAGGAAGAGCCACCTGCAAGATGGACG 228 0.10908934321516915 No Hit AGCTAGTAAGGCTTCCTCAGCTTCCCAAAGTGCTGGAGTTCGC 228 0.10908934321516915 No Hit AGCTAATTAAGATAGTGCTGGGATTATAGGTATGAGCCACTGT 225 0.10765395712023272 No Hit AGCTAGTAAGGCTGGCCTCAGCTTCCCAAAGTGCTGGAGTTCG 223 0.10669703305694178 No Hit AGCTAGTCCAGCGGGTTGAGGACACACATCACAAATAAGTTTC 219 0.10478318493035985 No Hit AGTTTGTCCATGTCGACGATCCATGTAAAGGGCATCAGTGACG 218 0.10430472289871438 No Hit AGCTAGCTTAAAAGCAGTGGCTCATATCTGTAATCCCAGCACT 217 0.1038262608670689 No Hit AGCTAGTCGTTAAGGCTAGCAGAGAGGGAAAAGAACTTGCATG 212 0.10143395070884151 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.009090778601264097 0.0 0.0 14 0.0 0.0 0.011483088759491491 0.0 0.0 15 0.0 0.0 0.03349234221518351 0.0 0.0 16 0.0 0.0 0.045932355037965965 0.0 0.0 17 0.0 0.0 0.07176930474682182 0.0 0.0 18 0.0 0.0 0.0765539250632766 0.0 0.0 19 0.0 0.0 0.1105247293101056 0.0 0.0 20 0.0 0.0 0.12679243838605186 0.0 0.0 21 0.0 0.0 0.157892470443008 0.0 0.0 22 0.0 0.0 0.18420788218350934 0.0 0.0 23 0.0 0.0 0.2502356425505854 0.0 0.0 24 0.0 0.0 0.2842064467974144 0.0 0.0 25 0.0 0.0 0.49472974072142506 0.0 0.0 26 0.0 0.0 0.6387468122467141 0.0 0.0 27 0.0 0.0 0.7621900164112477 0.0 0.0 28 0.0 0.0 0.8813270622909719 0.0 0.0 29 0.0 0.0 0.9770194686200676 0.0 0.0 30 0.0 0.0 1.2865844030946925 0.0 0.0 31 0.0 0.0 1.5172031023478132 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCGAG 20 0.0018034636 37.145157 37 AGTATGC 25 1.2021318E-4 37.145157 36 TCAGGGT 25 1.2209661E-4 37.047222 9 CAACTCG 25 1.2209661E-4 37.047222 9 ACGCGTT 25 1.2209661E-4 37.047222 9 TGGACGG 20 0.0018270073 37.04722 9 ATTACGA 65 0.0 37.04722 9 CTCCCGT 20 0.0018270073 37.04722 9 AGTGGTC 20 0.0018270073 37.04722 9 CCTCGGT 30 8.207277E-6 37.04722 9 AATGCCG 20 0.0018270073 37.04722 9 ACCGTTG 125 0.0 37.04722 9 ACTCCGC 20 0.0018270073 37.04722 9 ACTCCAT 45 2.566594E-9 37.04722 9 GGGGCGC 30 8.207277E-6 37.04722 9 GACGTGG 95 0.0 37.047215 9 CGTCGCT 25 1.2226903E-4 37.038345 8 CGTCGCG 85 0.0 37.038345 8 CAATGCA 25 1.2226903E-4 37.038345 8 CGATGCA 25 1.2226903E-4 37.038345 8 >>END_MODULE