FastQCFastQC Report
Thu 26 May 2016
SRR2099071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95583
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAGTCCTCCAGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT14311.4971281504033145No Hit
AGCTAGTCTCCTACCAAGCTGCCCGTTCTCATCTCTCTCAAGG11141.165479217015578No Hit
AGCTAGTCCGGCGCAAGAATCTTCTATCCTGTCCTGTGCCTTA10971.1476936275279077No Hit
AGCTAATTAAGATAGACTCCCAAAGTGCTGGGATTATAGGTAT9150.9572831988952011No Hit
AGCTAGTGGACGTGCAGCCAGGCCCGGCCCCGGGGCGATGGCA8240.8620779845788478No Hit
AGCTAGTGACAGTAGCGGCTCTACGAATCGCCGAAGGAGCGTC7790.8149984829938377No Hit
AGCTAGTCCAGCGGTTGAGGACACACATCACAAATAAGTTTCT6460.6758524005314752No Hit
AGCTGATCTACGAGATCAGTGCATCCACAGTCACGTGGTCAGT5130.5367063180691127No Hit
AGCTAGTCACCGTTGTCTGGAGCCCTCGGAACTCCAGTGTAAT4640.4854419718987686No Hit
AGCTAGTCTCAACGGAGCCGGCAGAATGTCCGTGGAGCTGAGC4020.4205768808260883No Hit
AGCTAGTAAGGCTCCTCAGCTTCCCAAAGTGCTGGAGTTCGCT4010.4195306696797548No Hit
AGCTAGTCCACCGACAGGGAGCCGGCCTGACCAGGCTTGTTCT3710.3881443352897482No Hit
AGCTAGTCGTTAAGCTAGCAGAGAGGGAAAAGAACTTGCATGT3570.37349737924107845No Hit
AGCTAGTCCACGATACAGTCCGACGATCCTTGTAGCTAGTTAG3260.3410648337047383No Hit
AGCTAGTCATTACGAGGCAGCAAGATTGCTTGAGCCCAGGAGT3150.3295565110950692No Hit
AGCTAGTCTTGAGGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT3100.32432545536340146No Hit
AGCTAGTCAGTAGACAGTCCGACGATCACGCAAGCTAGTCTGA3040.3180481884854001No Hit
AGCTAGTGGACGTGGCAGCCAGGCCCGGCCCCGGGGCGATGGC3000.3138633439000659No Hit
AGCCAGTTCAGTCGAAGGCCAGGACTTCTTCCCGCTGACCGTC2830.2960777544123955No Hit
AGCTAGTCCCTCGGTTGAGCATCCCGGGCCCTACTGTAGTGGT2760.2887542763880606No Hit
AGCTTGTCACTCCGTTACGCCATCGAGGAGCCGGCGAGCCTGA2610.2730611091930573No Hit
AACTAGTCTGGCAACGGTTTTGGCCAGAGCCATAGACATCACT2560.2678300534613896No Hit
AGCTAGCCGGGGCAGCTATTAACAAATATGCATGGAAGACTAT2520.26364520887605536No Hit
AGCTAGTCTCTGCGGAAAAGGGTCTGTACCAGATGCCCTCTCC2410.25213688626638625No Hit
AGCTAGTCCATAAACTGGAGTCACCCACGGAAGTTCGGCCAGG2320.2427209859493843No Hit
AACTCGTCCCAGCCCCCATTCCGGTGGCCGTGGCCTATCCCTG2260.23644371907138298No Hit
AGTTATTCTCGATATACCAAACGACCATAAGATGGGAATGTCC2230.2333050856323823No Hit
AGCCAGTTCAGTCGGAAGGCCAGGACTTCTTCCCGCTGACCGT2100.21970434073004613No Hit
AGCTAGTCAACGTGTGGAGGAAGAGCCACCTGCAAGATGGACG2060.21551949614471194No Hit
AGCTTGTCACTCCTTTACGCCATCGAGGAGCCGGCGAGCCTGA2000.2092422292667106No Hit
TGCTGGTCAGCTAGAAGGCGGCAGCTCTGGCAAAGCAGCTGGA1940.20296496238870926No Hit
AGCTAGTCACGCGTAGTCTTTTTTTTTTTTTTTTTTTTTTTTT1680.1757634725840369No Hit
AGCTAGTCCAAAAACGACTCACTATAGTGAGTCGTATTACCGG1620.1694862057060356No Hit
AGCTTGTCACTCCTTACGCCATCGAGGAGCCGGCGAGCCTGAA1610.16843999455970204No Hit
AGCTAGTCGCTCGCCTGACAGAGCTGCTGGCCAAGATCACCCA1540.16111651653536715No Hit
AGCTAGTCGAAAAGTGGAGCATCCCTTTGGAGGTGGTAACCAC1490.1558854608036994No Hit
AGCTAGTCTAAACGCAACTGGAGAAATCAAGTAGGAAGTAAAA1430.14960819392569807No Hit
AGCGAGTTAGCTACCTGCCTCTTGTAGCCACTGGGGACCTATG1420.14856198277936453No Hit
AGCTAGTCAAAGCAGAGAACAGCGGAATCGCCTGTGTGACCTT1360.1422847159013632No Hit
AGCTAGTCAGACGCAGTCCGACGATCCCGTTAGCTAGCACGAT1360.1422847159013632No Hit
AGCTAGTCTCACCAAAAAAAGAATGAGGCTCTGAAAGAGAAGG1340.14019229360869612No Hit
AGCTAGTCTGGACGATCCTGACACCATGCAAGCCCTAGAAAAG1340.14019229360869612No Hit
AGCTAGTCTCTATTAGTTAACACATTTCAAATGCATTTACTCT1340.14019229360869612No Hit
AGCGAGTTAGCTATCTGCCTCTTGTAGCCACTGGGGACCTATG1280.1339150267306948No Hit
AGCTAGTCACCGTGTGTCTGGAGCCCTCGGAACTCCAGTGTAA1270.13286881558436123No Hit
AGCTTGTCACTCCATTACGCCATCGAGGAGCCGGCGAGCCTGA1260.13182260443802768No Hit
AGCTAGTCCGCTGCTACAGTCCGACGATCCACCTAGCTTGTCC1200.12554533756002637No Hit
AGCTAGTCAACTCATCGAGAGGGCAAACCACGGAAGGTGGTTG1150.1203142818283586No Hit
AGCTAGTCTAGACCGATCAAACGAGCTAGTCTTTTTTGATCGT1130.11822185953569149No Hit
AGCTGGTCCCCCCGTCCGACGATCCGACCAGCTAGCGGGATCG1110.1161294372430244No Hit
AGCTTCTCTCGAGAGCTCCTCATTAAAATGCACATAATTCAGC1100.11508322609669083No Hit
AGCTAGTGACAGTCGAATCGCCGAAGGAGCGTCCGATGGACGC1100.11508322609669083No Hit
AGCTAGTCACACTAGTCCGACGATCTAAACAGCTAGTCTTTTT1090.11403701495035728No Hit
AGCTAGTCTACACCGATCAAACGAGCTAGTCTTTTTTGATCGT1070.11194459265769018No Hit
AGCTAGTAAGGCTGGCCTCAGCTTCCCAAAGTGCTGGAGTTCG1070.11194459265769018No Hit
AGCTGATCTACGAGGATCAGTGCATCCACAGTCACGTGGTCAG1050.10985217036502307No Hit
AGCTAATTAAGATAGTGCTGGGATTATAGGTATGAGCCACTGT1040.10880595921868952No Hit
AGCTAGTCCAGCGGGTTGAGGACACACATCACAAATAAGTTTC1030.10775974807235597No Hit
AGCTAGTCCGCAGGAAGAAAGCGAGGTGCTTAGAAAAGAATCG1010.10566732577968886No Hit
AGCTAGTCCAAGGGGAGAGCATCAGCCATGGCTCTGCGCTACC1010.10566732577968886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAC200.001818249637.06292713
TTGTCTG200.001818249637.06292713
TTGTAAT200.001818249637.06292713
AACGGTT251.2129638E-437.06292713
TGGCAGC200.001818249637.06292713
GTGTCTG308.13686E-637.06292714
AGTAGAG251.2129638E-437.06292713
TGTGTCT308.13686E-637.06292713
CGGAGCC200.001818249637.06292713
ATACAGT501.72804E-1037.06292713
GTTGAGC452.5247573E-937.06292314
TCTGCGG200.001827642337.0240979
CGTGGCA200.001827642337.02409711
AACCGAC200.001827642337.02409712
CTCACCA200.001827642337.0240978
TACCCAT200.001827642337.02409712
CCCTCCG200.001827642337.0240978
CTCAACG501.7462298E-1037.0240978
GTCGAAT251.22047895E-437.02409712
CAACGTG308.195664E-637.0240978
Produced by FastQC (version 0.11.5)