FastQCFastQC Report
Thu 26 May 2016
SRR2099070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178867
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGAAACCGACTCACTATAGTGAGTCGTATTACCGGC84524.725298685615569No Hit
ACTGCTAGGGGAACATTTGGCATACTTGGTCTTTAAGACCCA33071.848859767313143No Hit
ACTGATCGGCCCGGTCAGCCTTCAGCCGCTCTTCGGGATACT17280.9660809428234387No Hit
ACAGCCAGTTGGTCAACGTGTGCAGCAATGTAGCGGCGGTAT15780.8822197498700151No Hit
ACTGCTAGCCCAGGACGATCGGAGCGGTGTTGTCCAGCAGTC13150.7351831248916794No Hit
ACTGCTAGACAATCTGGCACTGCTAGTTTTTTTTTTTTTTTT11370.6356678425869501No Hit
GCTGCTTGCGGTTACAAATGGATCAAGCTCTTCGCAGTGCCT10940.611627633940302No Hit
ACTGCTAGCCCAGGGACGATCGGAGCGGTGTTGTCCAGCAGT10700.5982098430677543No Hit
GCTGCTGGTGTGCGCAGCGAAGGCTGCCTGCGCGCGCTTGGC10190.5696970374635904No Hit
ACTGCTAGACACTTCTACAATCCAACGATCTACGACTGCTAG8950.5003717846220935No Hit
ACTGGTAAAGCTGAGATGTGTCCAGCAGAGTCAAAAGAAAAC7250.4053290992748802No Hit
ACTGCTTTAGTTGACTGGGTTAGGGACCAGCTGAGCAACCTG7120.3980611292189169No Hit
GCTGCTAATCAGCGACGATCTCAGCGGCCAATTCATGGATCT7030.39302945764171143No Hit
ACTGCTAGATTGGCCTGTAATCCCTGCACTTTGGGAGCTTAA6650.3717846220935108No Hit
ACTGCTAGGGCGTCCGACGATCCAGGACTGTAGAACTCTGAA6610.36954832361475287No Hit
ACTGCTAGACAAAGTGGATATTATCTAGGCCTGCTACTGTAT5920.3309721748561781No Hit
ACTGCTCTCTTCTGCTGGGATTACAGGCGTGAGCCACTGCGC5440.30413659311108254No Hit
ACTGCTCTGCGCCCTGTAATCCCAGCACTTTGAGAGGCCGAG5330.29798677229449816No Hit
ACTGCTAGATGTAAAGATGGGCCTGCAGGTTATCGGAAGCTC5290.2957504738157402No Hit
ACTGCTAGGACGACAGTCCGACGATCCAATACTGCTAGTTTT5290.2957504738157402No Hit
ACTGCTAGAAACCCGACTCACTATAGTGAGTCGTATTACCGG5060.28289175756288193No Hit
ACCGCTATATGCGCCGGGGTCAGTTCGCCGGTGGATGGCAGG4840.27059211592971316No Hit
ACTGCTAATTAAAGCCTGGCCAACATGGTGAAACCCCATCTC4780.2672376682115762No Hit
ACTGCTAGAAACGCGACTCACTATAGTGAGTCGTATTACCGG4710.2633241458737498No Hit
ACTGCCAGTGTGGCTGACGGTGTGATCCAAGTCGATGTCTTG4600.25717432505716536No Hit
ACTGATCGGCCCGGGTCAGCCTTCAGCCGCTCTTCGGGATAC4530.25326080271933893No Hit
ACTGATCGGCCCAGGTCAGCCTTCAGCCGCTCTTCGGGATAC4230.23648856412865427No Hit
ACTGCTAGGGAGCAGTCCGACGATCACAAACTGCTAGTTTTT4210.23537041488927526No Hit
GCTGCCAGTCGATGAATAGGAATCCTATTCATAGATGATCCG4160.23257504179082783No Hit
ACTGCTAGACGGGCTCTTGGTGACGCTCCCTCCCTCTCACAT4130.23089781793175934No Hit
ACTGCCAGTGTGTCTGACGGTGTGATCCAAGTCGATGTCTTG4120.23033874331206985No Hit
ACTGCTAGAAACAGTGAGTCGTATTACCGGCCGACGATCAAA4120.23033874331206985No Hit
ACTGCTACTCCGGGATCACCTGAGGTCAGGAGTTGGAGACCA4060.22698429559393293No Hit
ACTGCTAGTTGGTAGGTTGGTTTCAAGCCAATCTCATATCCT4030.22530707173486447No Hit
ACTGCTAGCCCGACTGCCGCTGCCGCAGGGGCAGGTCGTCTC3870.2163618778198326No Hit
GCTGCTGGTGTGGCGCAGCGAAGGCTGCCTGCGCGCGCTTGG3870.2163618778198326No Hit
ACTGCTAGGGTTGGACTATAAATTGTGGTTAACTTTAGTCAA3780.21133020624262722No Hit
ACTACTAGACAATCTGGCACTGCTAGTTTTTTTTTTTTTTTT3720.20797575852449027No Hit
AATGCTGGTCAGCCACATGGATCTGAGATCGTCGGACTGTAG3710.20741668390480078No Hit
ACTGCTAGACCGATTTAAGCGAAACTACCGAAAGCTAGTGGG3600.20126686308821637No Hit
ACTGCTGTGAGTGAAGGTCGCCGTCGATCTACTTAACCGTGC3500.1956761168913215No Hit
ACTGCTAGAAACCCGGCCGACGATCAAAAACTAGCAGTGTTT3370.18840814683535811No Hit
ACTGCTAGACGGGGCTCTTGGTGACGCTCCCTCCCTCTCACA3360.18784907221566863No Hit
ACTGCTAGCGAAAGGGTGTTGGCAAGCTGGCCAGTGTGCCTG3350.18728999759597914No Hit
ACTGCTAGCATCTCCGACGATCGTCGGACTGTAGAACTCTGA3290.1839355498778422Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTGCCAGTCGACTATGAATAGGAATCCTATTCATAGATGAT3290.1839355498778422No Hit
ACTGCTAGACGCCAGTCCGACGATCGAACCCCTATAGTGAGT3260.18225832601877373No Hit
ACTGCTAGAAACTCGACTCACTATAGTGAGTCGTATTACCGG3120.17443128134312086No Hit
ACTGCTAGCGGAGAGATCACGACCATCCTGGCCAACATGGTA3100.17331313210374188No Hit
ACTGCTAGCTGAGTCCGACGATCACAAACTGCTAGTTTTTGA3020.16884053514622596No Hit
ACTGCTAGACTGGGCATTGAGGCAGCCAGCGCAGGGGCTTCT2880.16101349047057312No Hit
ACTGCTAGGCCGCTACAGTCCGACGATCAACGACTGTAGAAC2800.1565408935130572No Hit
ACTGCTAGCACATTCAACCTCTCATGGACTACCATAGAAAGA2760.15430459503429922No Hit
ACTGCCAGTGTGCTGACGGTGTGATCCAAGTCGATGTCTTGG2650.14815477421771484No Hit
ACTGCTAGAAACACCGGCCGACGATCAAAAACTAGCAGTGTT2620.14647755035864637No Hit
ACTACTAGATCCTCTCACTTTCCAGACTGGGCAGCCAGGCAG2580.14424125187988843No Hit
ACTGCTAGACCGTTGCAGTGCACTGAATGGTGCACTGCAAGT2560.14312310264050943No Hit
ACTGATAAAACGCAGATTATGGGCTACTCCAATCTTATCGAG2490.139209580302683No Hit
ACTGCTAGAAACTTGAGGCCGGTAATAGACTCACTATAGTGA2490.139209580302683No Hit
ACTACTAGATCCGCTCACTTTCCAGACTGGGCAGCCAGGCAG2430.13585513258454607No Hit
ACTGCTAGACCGAGTGCACTGAATGGTGCACTGCAAGTGGGT2380.1330597594860986No Hit
GCTGCCAGTCGATCTATGAATAGGAATCCTATTCATAGATGA2340.13082346100734063No Hit
ACTGCTAGCTCGGTTCTAGTCCGACGATCAGGAACTCTGAAC2310.12914623714827217No Hit
ACTGCTAGCTCGCGACGATCAAATACTGCTAGTTTTTGATCG2310.12914623714827217No Hit
ACTGCTAGTCAGCTACAGTCCGACGATCAGGGACTGCAGAAC2280.1274690132892037No Hit
AGTGGTAGAGTCATTAAGCCTCTTGCTGATGCTTTTGGTAAC2280.1274690132892037No Hit
ACTGCTAGATAAGCGGGGCCATTCGCAGGATGGGCGAGTCAG2250.12579178943013525No Hit
ACTGTGAGAGTCGGCCTTGATATCTGGAGGCCAGTAAGGGAC2250.12579178943013525No Hit
ACTGCTAGAGAGTGTGGAGGCAAGTCTTAAGAAACTTGCCTC2200.12299641633168779No Hit
ACTGCTAGACCGACTGAATGGTGCACTGCAAGTGGGTGATTA2190.1224373417119983No Hit
ACTGCTGACGTTTACAGTCCGACGATCCTTAACTGCTAGTGA2090.1168465955151034No Hit
ACTGGAAGCATGGGACAAGTGCACGTGCTGTGCCTGATGTGA2090.1168465955151034No Hit
ACTGCTAGTTGCCAAACACGGCTCCAGGGTGGCACCCCGGCC2080.11628752089541392No Hit
ACTGCTAGAAACCGCGACTCACTATAGTGAGTCGTATTACCG2050.11461029703634545No Hit
ACTGCTAGGCCCGTAAACTGTTTGGGGGCAAAAGTGAAATAT2050.11461029703634545No Hit
GCTGCTCGCATGGCGACGATCCATGATCGGTTCATCAGTGAC1990.1112558493182085No Hit
ACTGCTAGAAACGTTGAGGCCGGTAATAGACTCACTATAGTG1980.11069677469851902No Hit
ACAGCCAGTTGGTTCAACGTGTGCAGCAATGTAGCGGCGGTA1980.11069677469851902No Hit
ACTCATAGGTCACAACTCTCACATATGGCCATCCCACCCTCT1890.10566510312131361No Hit
ACTGCTGAAGTTTACAGTCCGACGATCCTTAACTGCTAGTGA1860.10398787926224513No Hit
GCTGCCAGTCGATATGAATAGGAATCCTATTCATAGATGATC1850.10342880464255565No Hit
GCTGCCAGTCGATAGGAATCCTATTCATAGATGATCCGGGAA1850.10342880464255565No Hit
ACTACTAGATCCCTCACTTTCCAGACTGGGCAGCCAGGCAGA1850.10342880464255565No Hit
ACTGCTAGGGGACATTTGGCATACTTGGTCTTTAAGACCCAT1850.10342880464255565No Hit
ACTGCTAGAAACATTACCGGCCGACGATCAAAAACTAGCAGT1830.10231065540317666No Hit
ACTGCTAGCATACCGACGATCACAAACTGCTAGTTTTTGATC1820.10175158078348717No Hit
ACTGCTAGCCACAGCAAATTCATTAAAAAATTTACATAACGC1810.10119250616379767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGC700.036.11419736
ACTTGTG200.002069918436.11419736
CAACCTG700.036.11419736
CCTGTTC200.002069918436.11419736
GACCCAT309.767951E-636.11419736
CACCCAG200.002069918436.11419736
TGCGCCC650.036.0333188
GCGGGTC453.3596734E-936.0333188
GTTTGTC200.00209283536.0333149
GTCGATA356.884893E-736.0333148
GATAAGC251.4366594E-436.0333148
GACAATT356.884893E-736.0333148
GACCGTT309.919369E-636.0333148
TGTGGGA200.00209283536.0333149
AGCTTAT200.00209283536.0333149
GGCGTAC356.884893E-736.0333148
GACCGCA356.884893E-736.0333148
GGCCGCT309.919369E-636.0333148
GATCCCC404.800313E-836.0333148
ACCGAGT309.919369E-636.0333149
Produced by FastQC (version 0.11.5)