##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099070_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 178867 Sequences flagged as poor quality 0 Sequence length 42 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.41604096898813 35.0 35.0 37.0 31.0 37.0 2 34.89559281477299 37.0 35.0 37.0 32.0 37.0 3 34.68122124259925 37.0 35.0 37.0 31.0 37.0 4 34.42151990026109 37.0 35.0 37.0 30.0 37.0 5 36.40579313120922 39.0 37.0 39.0 31.0 39.0 6 36.21921874912645 39.0 37.0 39.0 31.0 39.0 7 35.6242459481067 37.0 35.0 39.0 30.0 39.0 8 36.030737922590525 39.0 35.0 39.0 31.0 39.0 9 30.537852147126078 31.0 31.0 33.0 27.0 34.0 10 30.86773412647386 31.0 31.0 34.0 27.0 34.0 11 31.135150698563738 31.0 31.0 34.0 27.0 34.0 12 34.5986626935097 37.0 35.0 37.0 31.0 37.0 13 31.6417002577334 31.0 31.0 34.0 30.0 34.0 14 31.64699469438186 31.0 31.0 34.0 30.0 34.0 15 31.67052614512459 31.0 31.0 34.0 30.0 34.0 16 35.21832422973494 37.0 35.0 37.0 32.0 37.0 17 35.45127944226716 37.0 35.0 37.0 33.0 37.0 18 34.95123751167068 37.0 35.0 37.0 32.0 37.0 19 34.7850134457446 36.0 35.0 37.0 32.0 37.0 20 34.77016442384565 36.0 35.0 37.0 31.0 37.0 21 36.4641717029972 38.0 35.0 39.0 32.0 39.0 22 36.6146969536024 38.0 35.0 39.0 32.0 39.0 23 36.919756019835965 39.0 37.0 39.0 33.0 39.0 24 37.08987683586128 39.0 37.0 39.0 33.0 39.0 25 37.17852370755925 39.0 37.0 39.0 34.0 39.0 26 38.529644931709036 40.0 38.0 41.0 35.0 41.0 27 38.62989819251175 40.0 38.0 41.0 35.0 41.0 28 38.7020188184517 40.0 38.0 41.0 35.0 41.0 29 38.77356359753336 40.0 38.0 41.0 36.0 41.0 30 38.72661251097184 40.0 38.0 41.0 35.0 41.0 31 38.87063013300385 40.0 38.0 41.0 35.0 41.0 32 38.928170092862295 40.0 38.0 41.0 36.0 41.0 33 38.940721318074324 40.0 38.0 41.0 36.0 41.0 34 38.97383530779853 40.0 38.0 41.0 36.0 41.0 35 38.97872161997462 40.0 38.0 41.0 36.0 41.0 36 38.933319170109634 40.0 38.0 41.0 35.0 41.0 37 38.873520548787646 40.0 38.0 41.0 35.0 41.0 38 38.7925833160952 40.0 38.0 41.0 35.0 41.0 39 38.73294682641292 40.0 38.0 41.0 35.0 41.0 40 38.419507231630206 40.0 38.0 41.0 35.0 41.0 41 38.25210910900278 40.0 38.0 41.0 34.0 41.0 42 38.28909748584144 40.0 38.0 41.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.12180025646702575 1101 2 0.07206752485782175 1101 3 -0.15871126259932566 1101 4 0.032320114677816036 1101 5 -0.029798088356969288 1101 6 -0.23239249101863635 1101 7 0.4719727361336652 1101 8 0.19599854615054824 1101 9 0.37211738798789185 1101 10 0.4540607147225266 1101 11 0.16981378124454594 1101 12 0.3082511751017236 1101 13 0.32636416844213656 1101 14 0.40691556511898597 1101 15 -0.050677975223319294 1101 16 0.17740791715358029 1101 17 0.2579761724959866 1101 18 0.20668700752883495 1101 19 -0.25214345834378094 1101 20 -0.3206390198505744 1101 21 0.001979642329949627 1101 22 -0.04458836255259513 1101 23 -0.14391196582544552 1101 24 0.06849091728441437 1101 25 -0.20353252118557918 1101 26 0.05977303736145956 1101 27 0.12634737361727133 1101 28 0.11407443335151868 1101 29 0.6376720233851572 1101 30 0.4220450881234754 1101 31 0.07437942537409725 1101 32 0.24839889405307503 1101 33 0.3999203292032263 1101 34 0.4980060219804372 1101 35 0.38852786100905945 1101 36 0.3120006945516991 1101 37 0.29425348303049503 1101 38 0.2745084270835534 1101 39 0.20814435976654977 1101 40 0.2757140411276282 1101 41 0.1978859793414358 1101 42 0.275339088236052 1102 1 0.36941975029201046 1102 2 0.30263190471588786 1102 3 0.07319140722155737 1102 4 0.5264600268102981 1102 5 0.2838754500005791 1102 6 0.2551641159431739 1102 7 0.262347862865667 1102 8 0.09169303753277802 1102 9 0.04351988883277258 1102 10 0.40317865997430147 1102 11 0.1666099961820251 1102 12 0.08034985708888343 1102 13 0.19875348915859448 1102 14 0.1135597016515959 1102 15 0.3041445999710035 1102 16 0.17081109390248628 1102 17 -0.013101894418511506 1102 18 0.10107024510909213 1102 19 0.28530838349468013 1102 20 0.3793271849483446 1102 21 0.21924223104235097 1102 22 0.3539516847606876 1102 23 0.09164058571223421 1102 24 0.048862664496311936 1102 25 0.26413898945990866 1102 26 0.05068888731076271 1102 27 0.31704693427965225 1102 28 0.31754858002268804 1102 29 0.04403903926890251 1102 30 0.16845941728873015 1102 31 0.400219236120968 1102 32 0.2488179545464817 1102 33 0.29569592906804587 1102 34 0.37415336905715435 1102 35 0.4329212371496496 1102 36 0.2909260071573101 1102 37 0.350191299860505 1102 38 0.5062083256979477 1102 39 0.5267114432406999 1102 40 0.6828380695155971 1102 41 0.5427592473373792 1102 42 0.548052842882889 1103 1 0.3357047203080228 1103 2 0.36405159917766383 1103 3 0.1973604029613938 1103 4 0.20928096404743002 1103 5 0.3439112679801255 1103 6 0.19835734308886543 1103 7 0.6329282769432183 1103 8 0.2665227664557932 1103 9 0.1419514954862322 1103 10 0.33137989189571826 1103 11 0.021479341961203602 1103 12 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6 0.08937518574904146 1104 7 0.22084899875992647 1104 8 0.3723621825141876 1104 9 0.291738600109106 1104 10 0.2005506137124229 1104 11 0.5808112559920211 1104 12 0.2688824882330394 1104 13 -0.28922926590129805 1104 14 -0.09374100053758028 1104 15 -0.05925120754655211 1104 16 -0.0754456181999501 1104 17 -0.05049604972623456 1104 18 0.056421856013677996 1104 19 -0.278456589656912 1104 20 -0.27537386833542143 1104 21 -0.21432086272055528 1104 22 -0.370800483764711 1104 23 -0.27743469309817215 1104 24 -0.2320646382711402 1104 25 -0.18816635956942207 1104 26 -0.4079542353658141 1104 27 -0.49279404052414577 1104 28 -0.21668314240605469 1104 29 -0.21306029984716446 1104 30 -0.017891780563395798 1104 31 0.024922354667026525 1104 32 0.06988879304297058 1104 33 0.04486982415271967 1104 34 -0.17148892751450973 1104 35 0.061419275150477404 1104 36 -0.11120637615536566 1104 37 0.2863746951517072 1104 38 0.230379639204763 1104 39 -0.01525210487991302 1104 40 0.24868121284480083 1104 41 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-0.2555604646219223 2216 22 0.019796664185378177 2216 23 0.38015220529754856 2216 24 0.19982781568013763 2216 25 0.12865999218340107 2216 26 0.285227582816006 2216 27 0.19607999393812747 2216 28 -0.03330524579286731 2216 29 0.22013191643328867 2216 30 0.20557449988817922 2216 31 0.036304559063935926 2216 32 0.08968231651296321 2216 33 0.10473316342782368 2216 34 0.15768516636546792 2216 35 -0.12227427802837099 2216 36 0.20782957155704906 2216 37 0.5100823600358453 2216 38 0.2734389083669768 2216 39 0.35531013516762044 2216 40 0.6941097630527651 2216 41 0.6932870488601566 2216 42 0.7688150240649279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 16.0 26 104.0 27 400.0 28 1018.0 29 1796.0 30 2824.0 31 4211.0 32 6581.0 33 9628.0 34 13408.0 35 20402.0 36 30934.0 37 48675.0 38 38869.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.149612199363638 93.37076201287263 0.5910674443183153 0.8885583434454144 2 1.5509250199875881 1.3345558841782166 1.1891916068902668 95.92532748894392 3 0.48248409423814476 3.388011136828687 94.88332047454519 1.2461842943879775 4 95.41551122640666 3.054275874407371 0.6613963681903569 0.8688165309956167 5 1.8544608813301575 3.334898751020317 1.157290932877126 93.65334943477241 6 1.248979683114734 1.7594176646204422 89.35851419498395 7.633088457280869 7 4.425708918507917 86.32033276679488 4.156230432060113 5.097727882637088 8 87.06245422576552 6.0564553550962446 5.527011690250298 1.3540787288879446 9 21.601736688095517 36.92420453083125 19.930510096178054 21.54354868489518 10 29.71225998367274 22.370024938213618 21.726440097963565 26.191274980150077 11 23.924263690299778 25.78523857720418 22.895359305713214 27.39513842678283 12 27.710519988145233 22.094044097499875 19.85729543535517 30.338140478999726 13 33.235475032153445 23.27126321087066 19.794777162668456 23.69848459430744 14 33.527530527351615 22.166994677282283 19.748848235451984 24.556626559914122 15 20.628983562562897 36.066756122106675 23.582690372358268 19.721569942972156 16 28.387731597956012 26.805541578611923 21.100153187302226 23.70657363612984 17 21.03138662462402 35.42428410094708 24.778325673968222 18.766003600460678 18 30.420681209423527 24.493230963658018 17.8852423264683 27.20084550045015 19 27.034295362801775 29.622382001364212 22.46307126323676 20.880251372597254 20 24.831299233508698 23.890376648571284 22.553629232893716 28.724694885026302 21 21.113453012573586 21.654078169813324 31.05100437755427 26.181464440058814 22 25.62853964118591 29.196553864044233 23.542632235124422 21.632274259645435 23 21.04468683435178 26.71202625414414 27.941431342841327 24.30185556866275 24 19.48822309313624 32.66393465535845 26.026041695785135 21.821800555720174 25 28.839960638726126 24.805989175649685 24.00925884510444 22.34479134051975 26 25.106363946395927 24.759737682188444 27.76420468839976 22.369693683015875 27 29.355890130655737 23.22395970190141 25.688360625492685 21.731789541950164 28 25.057724454482937 28.460252589913175 23.33745184969838 23.144571105905506 29 29.909374004148333 23.814901574913204 25.79626202709275 20.479462393845708 30 27.03181693660653 21.183896414654463 32.343584898276376 19.440701750462637 31 21.54430181590482 24.13565614097862 28.869420341712136 25.45062170140442 32 24.985603828542995 22.917586810311573 31.736429861293587 20.360379499851845 33 22.502879250388556 23.91790502387262 33.908624333299784 19.670591392439032 34 23.32459313344552 27.888878328590515 26.298870110193604 22.487658427770356 35 22.5010762186429 24.928578217334667 31.014105452654768 21.556240111367664 36 22.33515592678318 27.407668310355238 29.976071472498965 20.281104290362617 37 27.026785265029325 29.324022877333437 24.80222735328484 18.846964504352396 38 22.963430929126112 27.013926548776467 25.786198683938345 24.23644383815908 39 22.715531700771553 22.763054903276306 26.728726378172873 27.792687017779265 40 26.442321132052264 22.964760344625155 26.76547710233086 23.82744142099172 41 27.00743442278019 25.145181652405668 26.38630944031421 21.461074484499928 42 22.887605458987707 23.3365574384578 26.677699442583908 27.09813765997059 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 6.5 7 12.0 8 45.0 9 78.0 10 78.0 11 139.0 12 200.0 13 397.0 14 594.0 15 739.5 16 885.0 17 885.0 18 951.5 19 1018.0 20 923.0 21 828.0 22 828.0 23 1076.5 24 1325.0 25 1688.5 26 2052.0 27 2474.0 28 2896.0 29 2896.0 30 3955.0 31 5014.0 32 4493.5 33 3973.0 34 3973.0 35 4432.5 36 4892.0 37 4980.5 38 5069.0 39 6565.0 40 8061.0 41 8061.0 42 9507.0 43 10953.0 44 13836.5 45 16720.0 46 20463.5 47 24207.0 48 24207.0 49 19203.5 50 14200.0 51 14699.0 52 15198.0 53 15198.0 54 16712.0 55 18226.0 56 13854.5 57 9483.0 58 9577.5 59 9672.0 60 9672.0 61 9984.0 62 10296.0 63 7020.0 64 3744.0 65 3400.5 66 3057.0 67 3057.0 68 2367.5 69 1678.0 70 1446.5 71 1215.0 72 1215.0 73 1928.5 74 2642.0 75 1494.5 76 347.0 77 272.0 78 197.0 79 197.0 80 134.5 81 72.0 82 67.0 83 62.0 84 62.0 85 31.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021244835548200617 2 0.00335444771813694 3 5.590746196894899E-4 4 0.00167722385906847 5 5.590746196894899E-4 6 5.590746196894899E-4 7 0.00167722385906847 8 0.0 9 0.07603414827777064 10 0.013976865492237249 11 0.02012668630882164 12 0.02012668630882164 13 0.02068576092851113 14 0.00614982081658439 15 0.003913522337826429 16 5.590746196894899E-4 17 5.590746196894899E-4 18 0.021244835548200617 19 0.00503167157720541 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00614982081658439 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.00167722385906847 32 0.0 33 5.590746196894899E-4 34 0.0 35 0.0 36 5.590746196894899E-4 37 0.0 38 0.0 39 0.003913522337826429 40 0.00335444771813694 41 0.3589259058406526 42 0.00335444771813694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 178867.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.768140573722375 #Duplication Level Percentage of deduplicated Percentage of total 1 67.05431853637782 10.573219207567634 2 9.92412423769678 3.129699721021765 3 4.822011062260672 2.281024448333119 4 2.6804708551978442 1.6906416499410177 5 1.8862572684725571 1.4871384883740433 6 1.3686001985533967 1.2948168192008587 7 1.1310452418096724 1.248413625766631 8 0.8225783576797617 1.0376424941436935 9 0.6878456956460077 0.9761442859778495 >10 7.669124946816054 29.455964487580154 >50 1.3437810239682315 13.847160180469286 >100 0.535385051765707 17.159677302129513 >500 0.042547156431711815 4.245612661921987 >1k 0.028364770954474542 6.847545941956873 >5k 0.0035455963693093178 4.725298685615569 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTGCTAGAAACCGACTCACTATAGTGAGTCGTATTACCGGC 8452 4.725298685615569 No Hit ACTGCTAGGGGAACATTTGGCATACTTGGTCTTTAAGACCCA 3307 1.848859767313143 No Hit ACTGATCGGCCCGGTCAGCCTTCAGCCGCTCTTCGGGATACT 1728 0.9660809428234387 No Hit ACAGCCAGTTGGTCAACGTGTGCAGCAATGTAGCGGCGGTAT 1578 0.8822197498700151 No Hit ACTGCTAGCCCAGGACGATCGGAGCGGTGTTGTCCAGCAGTC 1315 0.7351831248916794 No Hit ACTGCTAGACAATCTGGCACTGCTAGTTTTTTTTTTTTTTTT 1137 0.6356678425869501 No Hit GCTGCTTGCGGTTACAAATGGATCAAGCTCTTCGCAGTGCCT 1094 0.611627633940302 No Hit ACTGCTAGCCCAGGGACGATCGGAGCGGTGTTGTCCAGCAGT 1070 0.5982098430677543 No Hit GCTGCTGGTGTGCGCAGCGAAGGCTGCCTGCGCGCGCTTGGC 1019 0.5696970374635904 No Hit ACTGCTAGACACTTCTACAATCCAACGATCTACGACTGCTAG 895 0.5003717846220935 No Hit ACTGGTAAAGCTGAGATGTGTCCAGCAGAGTCAAAAGAAAAC 725 0.4053290992748802 No Hit ACTGCTTTAGTTGACTGGGTTAGGGACCAGCTGAGCAACCTG 712 0.3980611292189169 No Hit GCTGCTAATCAGCGACGATCTCAGCGGCCAATTCATGGATCT 703 0.39302945764171143 No Hit ACTGCTAGATTGGCCTGTAATCCCTGCACTTTGGGAGCTTAA 665 0.3717846220935108 No Hit ACTGCTAGGGCGTCCGACGATCCAGGACTGTAGAACTCTGAA 661 0.36954832361475287 No Hit ACTGCTAGACAAAGTGGATATTATCTAGGCCTGCTACTGTAT 592 0.3309721748561781 No Hit ACTGCTCTCTTCTGCTGGGATTACAGGCGTGAGCCACTGCGC 544 0.30413659311108254 No Hit ACTGCTCTGCGCCCTGTAATCCCAGCACTTTGAGAGGCCGAG 533 0.29798677229449816 No Hit ACTGCTAGATGTAAAGATGGGCCTGCAGGTTATCGGAAGCTC 529 0.2957504738157402 No Hit ACTGCTAGGACGACAGTCCGACGATCCAATACTGCTAGTTTT 529 0.2957504738157402 No Hit ACTGCTAGAAACCCGACTCACTATAGTGAGTCGTATTACCGG 506 0.28289175756288193 No Hit ACCGCTATATGCGCCGGGGTCAGTTCGCCGGTGGATGGCAGG 484 0.27059211592971316 No Hit ACTGCTAATTAAAGCCTGGCCAACATGGTGAAACCCCATCTC 478 0.2672376682115762 No Hit ACTGCTAGAAACGCGACTCACTATAGTGAGTCGTATTACCGG 471 0.2633241458737498 No Hit ACTGCCAGTGTGGCTGACGGTGTGATCCAAGTCGATGTCTTG 460 0.25717432505716536 No Hit ACTGATCGGCCCGGGTCAGCCTTCAGCCGCTCTTCGGGATAC 453 0.25326080271933893 No Hit ACTGATCGGCCCAGGTCAGCCTTCAGCCGCTCTTCGGGATAC 423 0.23648856412865427 No Hit ACTGCTAGGGAGCAGTCCGACGATCACAAACTGCTAGTTTTT 421 0.23537041488927526 No Hit GCTGCCAGTCGATGAATAGGAATCCTATTCATAGATGATCCG 416 0.23257504179082783 No Hit ACTGCTAGACGGGCTCTTGGTGACGCTCCCTCCCTCTCACAT 413 0.23089781793175934 No Hit ACTGCCAGTGTGTCTGACGGTGTGATCCAAGTCGATGTCTTG 412 0.23033874331206985 No Hit ACTGCTAGAAACAGTGAGTCGTATTACCGGCCGACGATCAAA 412 0.23033874331206985 No Hit ACTGCTACTCCGGGATCACCTGAGGTCAGGAGTTGGAGACCA 406 0.22698429559393293 No Hit ACTGCTAGTTGGTAGGTTGGTTTCAAGCCAATCTCATATCCT 403 0.22530707173486447 No Hit ACTGCTAGCCCGACTGCCGCTGCCGCAGGGGCAGGTCGTCTC 387 0.2163618778198326 No Hit GCTGCTGGTGTGGCGCAGCGAAGGCTGCCTGCGCGCGCTTGG 387 0.2163618778198326 No Hit ACTGCTAGGGTTGGACTATAAATTGTGGTTAACTTTAGTCAA 378 0.21133020624262722 No Hit ACTACTAGACAATCTGGCACTGCTAGTTTTTTTTTTTTTTTT 372 0.20797575852449027 No Hit AATGCTGGTCAGCCACATGGATCTGAGATCGTCGGACTGTAG 371 0.20741668390480078 No Hit ACTGCTAGACCGATTTAAGCGAAACTACCGAAAGCTAGTGGG 360 0.20126686308821637 No Hit ACTGCTGTGAGTGAAGGTCGCCGTCGATCTACTTAACCGTGC 350 0.1956761168913215 No Hit ACTGCTAGAAACCCGGCCGACGATCAAAAACTAGCAGTGTTT 337 0.18840814683535811 No Hit ACTGCTAGACGGGGCTCTTGGTGACGCTCCCTCCCTCTCACA 336 0.18784907221566863 No Hit ACTGCTAGCGAAAGGGTGTTGGCAAGCTGGCCAGTGTGCCTG 335 0.18728999759597914 No Hit ACTGCTAGCATCTCCGACGATCGTCGGACTGTAGAACTCTGA 329 0.1839355498778422 Illumina DpnII expression Sequencing Primer (100% over 24bp) GCTGCCAGTCGACTATGAATAGGAATCCTATTCATAGATGAT 329 0.1839355498778422 No Hit ACTGCTAGACGCCAGTCCGACGATCGAACCCCTATAGTGAGT 326 0.18225832601877373 No Hit ACTGCTAGAAACTCGACTCACTATAGTGAGTCGTATTACCGG 312 0.17443128134312086 No Hit ACTGCTAGCGGAGAGATCACGACCATCCTGGCCAACATGGTA 310 0.17331313210374188 No Hit ACTGCTAGCTGAGTCCGACGATCACAAACTGCTAGTTTTTGA 302 0.16884053514622596 No Hit ACTGCTAGACTGGGCATTGAGGCAGCCAGCGCAGGGGCTTCT 288 0.16101349047057312 No Hit ACTGCTAGGCCGCTACAGTCCGACGATCAACGACTGTAGAAC 280 0.1565408935130572 No Hit ACTGCTAGCACATTCAACCTCTCATGGACTACCATAGAAAGA 276 0.15430459503429922 No Hit ACTGCCAGTGTGCTGACGGTGTGATCCAAGTCGATGTCTTGG 265 0.14815477421771484 No Hit ACTGCTAGAAACACCGGCCGACGATCAAAAACTAGCAGTGTT 262 0.14647755035864637 No Hit ACTACTAGATCCTCTCACTTTCCAGACTGGGCAGCCAGGCAG 258 0.14424125187988843 No Hit ACTGCTAGACCGTTGCAGTGCACTGAATGGTGCACTGCAAGT 256 0.14312310264050943 No Hit ACTGATAAAACGCAGATTATGGGCTACTCCAATCTTATCGAG 249 0.139209580302683 No Hit ACTGCTAGAAACTTGAGGCCGGTAATAGACTCACTATAGTGA 249 0.139209580302683 No Hit ACTACTAGATCCGCTCACTTTCCAGACTGGGCAGCCAGGCAG 243 0.13585513258454607 No Hit ACTGCTAGACCGAGTGCACTGAATGGTGCACTGCAAGTGGGT 238 0.1330597594860986 No Hit GCTGCCAGTCGATCTATGAATAGGAATCCTATTCATAGATGA 234 0.13082346100734063 No Hit ACTGCTAGCTCGGTTCTAGTCCGACGATCAGGAACTCTGAAC 231 0.12914623714827217 No Hit ACTGCTAGCTCGCGACGATCAAATACTGCTAGTTTTTGATCG 231 0.12914623714827217 No Hit ACTGCTAGTCAGCTACAGTCCGACGATCAGGGACTGCAGAAC 228 0.1274690132892037 No Hit AGTGGTAGAGTCATTAAGCCTCTTGCTGATGCTTTTGGTAAC 228 0.1274690132892037 No Hit ACTGCTAGATAAGCGGGGCCATTCGCAGGATGGGCGAGTCAG 225 0.12579178943013525 No Hit ACTGTGAGAGTCGGCCTTGATATCTGGAGGCCAGTAAGGGAC 225 0.12579178943013525 No Hit ACTGCTAGAGAGTGTGGAGGCAAGTCTTAAGAAACTTGCCTC 220 0.12299641633168779 No Hit ACTGCTAGACCGACTGAATGGTGCACTGCAAGTGGGTGATTA 219 0.1224373417119983 No Hit ACTGCTGACGTTTACAGTCCGACGATCCTTAACTGCTAGTGA 209 0.1168465955151034 No Hit ACTGGAAGCATGGGACAAGTGCACGTGCTGTGCCTGATGTGA 209 0.1168465955151034 No Hit ACTGCTAGTTGCCAAACACGGCTCCAGGGTGGCACCCCGGCC 208 0.11628752089541392 No Hit ACTGCTAGAAACCGCGACTCACTATAGTGAGTCGTATTACCG 205 0.11461029703634545 No Hit ACTGCTAGGCCCGTAAACTGTTTGGGGGCAAAAGTGAAATAT 205 0.11461029703634545 No Hit GCTGCTCGCATGGCGACGATCCATGATCGGTTCATCAGTGAC 199 0.1112558493182085 No Hit ACTGCTAGAAACGTTGAGGCCGGTAATAGACTCACTATAGTG 198 0.11069677469851902 No Hit ACAGCCAGTTGGTTCAACGTGTGCAGCAATGTAGCGGCGGTA 198 0.11069677469851902 No Hit ACTCATAGGTCACAACTCTCACATATGGCCATCCCACCCTCT 189 0.10566510312131361 No Hit ACTGCTGAAGTTTACAGTCCGACGATCCTTAACTGCTAGTGA 186 0.10398787926224513 No Hit GCTGCCAGTCGATATGAATAGGAATCCTATTCATAGATGATC 185 0.10342880464255565 No Hit GCTGCCAGTCGATAGGAATCCTATTCATAGATGATCCGGGAA 185 0.10342880464255565 No Hit ACTACTAGATCCCTCACTTTCCAGACTGGGCAGCCAGGCAGA 185 0.10342880464255565 No Hit ACTGCTAGGGGACATTTGGCATACTTGGTCTTTAAGACCCAT 185 0.10342880464255565 No Hit ACTGCTAGAAACATTACCGGCCGACGATCAAAAACTAGCAGT 183 0.10231065540317666 No Hit ACTGCTAGCATACCGACGATCACAAACTGCTAGTTTTTGATC 182 0.10175158078348717 No Hit ACTGCTAGCCACAGCAAATTCATTAAAAAATTTACATAACGC 181 0.10119250616379767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0016772238590684698 0.0 0.0 0.0 0.0 8 0.0016772238590684698 0.0 0.0 0.0 0.0 9 0.0016772238590684698 0.0 0.0 0.0 0.0 10 0.0016772238590684698 0.0 0.0 0.0 0.0 11 0.0016772238590684698 0.0 0.0 0.0 0.0 12 0.0016772238590684698 0.0 0.032426327941990414 0.0 0.0 13 0.0016772238590684698 0.0 0.045844118814538176 0.0 0.0 14 0.0016772238590684698 0.0 0.13473698334516707 0.0 0.0 15 0.0016772238590684698 0.0 0.13529605796485658 0.0 0.0 16 0.0016772238590684698 0.0 0.18281740063846322 0.0 0.0 17 0.0016772238590684698 0.0 0.19064444531411606 0.0 0.0 18 0.0016772238590684698 0.0 0.2051803854260428 0.0 0.0 19 0.0016772238590684698 0.0 0.4590002627650713 0.0 0.0 20 0.0016772238590684698 0.0 0.5792013059983115 0.0 0.0 21 0.0016772238590684698 0.0 0.7262379309766475 0.0 0.0 22 0.0016772238590684698 0.0 1.078454941381026 0.0 0.0 23 0.0016772238590684698 0.0 1.2417047303303572 0.0 0.0 24 0.0016772238590684698 0.0 1.3529605796485658 0.0 0.0 25 0.0016772238590684698 0.0 1.4563893842911213 0.0 0.0 26 0.0016772238590684698 0.0 1.6464747549855479 0.0 0.0 27 0.0016772238590684698 0.0 2.0680170182314233 0.0 0.0 28 0.0016772238590684698 0.0 2.2139354939703804 0.0 0.0 29 0.0016772238590684698 0.0 2.354822298132132 0.0 0.0 30 0.0016772238590684698 0.0 2.6064058769924023 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGC 70 0.0 36.114197 36 ACTTGTG 20 0.0020699184 36.114197 36 CAACCTG 70 0.0 36.114197 36 CCTGTTC 20 0.0020699184 36.114197 36 GACCCAT 30 9.767951E-6 36.114197 36 CACCCAG 20 0.0020699184 36.114197 36 TGCGCCC 65 0.0 36.033318 8 GCGGGTC 45 3.3596734E-9 36.033318 8 GTTTGTC 20 0.002092835 36.033314 9 GTCGATA 35 6.884893E-7 36.033314 8 GATAAGC 25 1.4366594E-4 36.033314 8 GACAATT 35 6.884893E-7 36.033314 8 GACCGTT 30 9.919369E-6 36.033314 8 TGTGGGA 20 0.002092835 36.033314 9 AGCTTAT 20 0.002092835 36.033314 9 GGCGTAC 35 6.884893E-7 36.033314 8 GACCGCA 35 6.884893E-7 36.033314 8 GGCCGCT 30 9.919369E-6 36.033314 8 GATCCCC 40 4.800313E-8 36.033314 8 ACCGAGT 30 9.919369E-6 36.033314 9 >>END_MODULE