FastQCFastQC Report
Thu 26 May 2016
SRR2099069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93697
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGAAACCGACTCACTATAGTGAGTCGTATTACCGGC36813.92862098039425No Hit
ACTGCTAGGGGAACATTTGGCATACTTGGTCTTTAAGACCCA14951.5955686948354804No Hit
ACAGCCAGTTGGTCAACGTGTGCAGCAATGTAGCGGCGGTAT8390.8954395551618515No Hit
ACTGATCGGCCCGGTCAGCCTTCAGCCGCTCTTCGGGATACT8050.8591523741421816No Hit
ACTGCTAGCCCAGGACGATCGGAGCGGTGTTGTCCAGCAGTC6090.6499674482640853No Hit
GCTGCTTGCGGTTACAAATGGATCAAGCTCTTCGCAGTGCCT5240.5592494957149108No Hit
ACTGCTAGCCCAGGGACGATCGGAGCGGTGTTGTCCAGCAGT5050.5389713651450954No Hit
ACTGCTAGACAATCTGGCACTGCTAGTTTTTTTTTTTTTTTT4830.515491424485309No Hit
GCTGCTGGTGTGCGCAGCGAAGGCTGCCTGCGCGCGCTTGGC4790.5112223443653479No Hit
ACTGCTAGACACTTCTACAATCCAACGATCTACGACTGCTAG4160.44398433247595975No Hit
ACTGCTTTAGTTGACTGGGTTAGGGACCAGCTGAGCAACCTG3730.39809172118637737No Hit
ACTGGTAAAGCTGAGATGTGTCCAGCAGAGTCAAAAGAAAAC3300.352199109896795No Hit
ACTGCTAGATTGGCCTGTAATCCCTGCACTTTGGGAGCTTAA3060.3265846291770281No Hit
GCTGCTAATCAGCGACGATCTCAGCGGCCAATTCATGGATCT3020.32231554905706694No Hit
ACTGCTAGGGCGTCCGACGATCCAGGACTGTAGAACTCTGAA2970.31697919890711546No Hit
ACTGCTAGCCCGACTGCCGCTGCCGCAGGGGCAGGTCGTCTC2770.29563379830730974No Hit
ACTGCTAGGACGACAGTCCGACGATCCAATACTGCTAGTTTT2460.2625484273776108No Hit
ACTGCTAGACAAAGTGGATATTATCTAGGCCTGCTACTGTAT2450.2614811573476205No Hit
ACTGCTCTGCGCCCTGTAATCCCAGCACTTTGAGAGGCCGAG2440.2604138873176302No Hit
ACTGCTCTCTTCTGCTGGGATTACAGGCGTGAGCCACTGCGC2390.2550775371676788No Hit
ACTGCTAGCTGAGTCCGACGATCACAAACTGCTAGTTTTTGA2280.24333756683778562No Hit
ACTGCTAGATGTAAAGATGGGCCTGCAGGTTATCGGAAGCTC2240.23906848671782446No Hit
ACCGCTATATGCGCCGGGGTCAGTTCGCCGGTGGATGGCAGG2240.23906848671782446No Hit
ACTGCTAGAAACCCGACTCACTATAGTGAGTCGTATTACCGG2230.2380012166878342No Hit
ACTGATCGGCCCGGGTCAGCCTTCAGCCGCTCTTCGGGATAC2170.2315975965078925No Hit
ACTGATCGGCCCAGGTCAGCCTTCAGCCGCTCTTCGGGATAC2150.2294630564479119No Hit
ACTGCTAGAAACGCGACTCACTATAGTGAGTCGTATTACCGG2140.2283957864179216No Hit
ACTGCCAGTGTGGCTGACGGTGTGATCCAAGTCGATGTCTTG2120.22626124635794104No Hit
ACTGCTAGGCCCGTAAACTGTTTGGGGGCAAAAGTGAAATAT2030.21665581608802842No Hit
ACTACTAGACAATCTGGCACTGCTAGTTTTTTTTTTTTTTTT2020.21558854605803812No Hit
ACTGCTACTCCGGGATCACCTGAGGTCAGGAGTTGGAGACCA1940.20705038581811586No Hit
ACTGCTAGTTGGTAGGTTGGTTTCAAGCCAATCTCATATCCT1930.20598311578812556No Hit
ACTGCCAGTGTGTCTGACGGTGTGATCCAAGTCGATGTCTTG1910.203848575728145No Hit
ACTGCTAATTAAAGCCTGGCCAACATGGTGAAACCCCATCTC1890.2017140356681644No Hit
GCTGCTGGTGTGGCGCAGCGAAGGCTGCCTGCGCGCGCTTGG1890.2017140356681644No Hit
ACTGCTAGAAACAGTGAGTCGTATTACCGGCCGACGATCAAA1820.1942431454582324No Hit
ACTGCTAGACGGGCTCTTGGTGACGCTCCCTCCCTCTCACAT1810.1931758754282421No Hit
ACTGCTAGGGAGCAGTCCGACGATCACAAACTGCTAGTTTTT1770.18890679530828094No Hit
ACTGCTAGGGTTGGACTATAAATTGTGGTTAACTTTAGTCAA1760.18783952527829065No Hit
ACTGCTGTGAGTGAAGGTCGCCGTCGATCTACTTAACCGTGC1740.18570498521831008No Hit
ACTGCTAGTGTTGAAGATCCAGCTCAGTGGCCCAGGTGAAAG1710.18250317512833922No Hit
ACTGCTAGACCGATTTAAGCGAAACTACCGAAAGCTAGTGGG1690.18036863506835865No Hit
GCTGCCAGTCGATGAATAGGAATCCTATTCATAGATGATCCG1640.17503228491840722No Hit
ACTGCTGACGTTTACAGTCCGACGATCCTTAACTGCTAGTGA1620.17289774485842663No Hit
ACTGCTAGACGCCAGTCCGACGATCGAACCCCTATAGTGAGT1530.16329231458851404No Hit
ACTGCTAGACGGGGCTCTTGGTGACGCTCCCTCCCTCTCACA1500.16009050449854317No Hit
ACTACTAGATCCTCTCACTTTCCAGACTGGGCAGCCAGGCAG1440.15368688431860145No Hit
GCTGCCAGTCGACTATGAATAGGAATCCTATTCATAGATGAT1420.15155234425862088No Hit
ACTGCTAGAAACCCGGCCGACGATCAAAAACTAGCAGTGTTT1410.15048507422863058No Hit
ACTGCTAGACTGGGCATTGAGGCAGCCAGCGCAGGGGCTTCT1390.14835053416865002No Hit
ACTACTAGATCCGCTCACTTTCCAGACTGGGCAGCCAGGCAG1320.140879643958718No Hit
ACTGCTAGGCCGCTACAGTCCGACGATCAACGACTGTAGAAC1290.13767783386874713No Hit
ACTGCTAGAAACTCGACTCACTATAGTGAGTCGTATTACCGG1250.133408753748786No Hit
ACTGCTAGAAACTTGAGGCCGGTAATAGACTCACTATAGTGA1210.12913967362882484No Hit
ACTGCTAGAAGAAAAGAGAGGCCCATTGGACTTGCAAACTTT1200.12807240359883454No Hit
AATGCTGGTCAGCCACATGGATCTGAGATCGTCGGACTGTAG1190.12700513356884424No Hit
ACTGCTAGGACTAGAGAGAGTGGGAGGGAAAGGAGGAGCGGG1180.12593786353885397No Hit
ACTGCTAGCACATTCAACCTCTCATGGACTACCATAGAAAGA1180.12593786353885397No Hit
ACTGCTAGAAACACCGGCCGACGATCAAAAACTAGCAGTGTT1170.12487059350886369No Hit
ACTGCCAGTGTGCTGACGGTGTGATCCAAGTCGATGTCTTGG1140.12166878341889281No Hit
ACTGCTAGTCAGCTACAGTCCGACGATCAGGGACTGCAGAAC1120.11953424335891223No Hit
ACTGCTAGACCGAGTGCACTGAATGGTGCACTGCAAGTGGGT1100.11739970329893165No Hit
ACTGCTAGACCGTTGCAGTGCACTGAATGGTGCACTGCAAGT1070.1141978932089608No Hit
ACTGATAAAACGCAGATTATGGGCTACTCCAATCTTATCGAG1040.11099608311898994No Hit
ACTGCTAGAGAGTGTGGAGGCAAGTCTTAAGAAACTTGCCTC1030.10992881308899964No Hit
ACTGCTAGTTATAAGAAGCCCAAGTTTGAATTGGGCAAGCTC1000.10672700299902878No Hit
ACTGCTCGGTATTCTGGCGTTCGCGCTCGGCCCGCTCCTGTT990.10565973296903851No Hit
ACTGCTAGTCGAGAAAAAGCTGGGTGGGAGATGATGAAATAG990.10565973296903851No Hit
ACTGCTAGACAAGGAGAGGCCCATTGGTCAGGCAAAAGTTAT990.10565973296903851No Hit
ACTGTGAGAGTCGGCCTTGATATCTGGAGGCCAGTAAGGGAC980.10459246293904821No Hit
ACTACTAGATCCCTCACTTTCCAGACTGGGCAGCCAGGCAGA970.10352519290905793No Hit
ACTGCTAGATAAGCGGGGCCATTCGCAGGATGGGCGAGTCAG960.10245792287906763No Hit
ACTGCTAGTTGCCAAACACGGCTCCAGGGTGGCACCCCGGCC950.10139065284907735No Hit
ACTGCTAGCTCGGTTCTAGTCCGACGATCAGGAACTCTGAAC950.10139065284907735No Hit
ACTGCTAGACCGACTGAATGGTGCACTGCAAGTGGGTGATTA950.10139065284907735No Hit
ACTGCTAGGAGCAAAGAAGGCGTATGCAGAATTCTACAGAAA940.10032338281908705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAC200.002092832736.015499
AACGCGA251.4356103E-436.0154910
AAACCGC200.002092832736.015499
GTTGAGG251.4356103E-436.0154913
CGATATG200.002092832736.0154910
GGGCATT251.4356103E-436.0154912
GTGTCCG251.4356103E-436.0154911
CACATTC251.4356103E-436.015499
AACCGCG200.002092832736.0154910
GAAACTC251.4356103E-436.015498
GAGTGTG309.902513E-636.0154910
GAAACGA251.4356103E-436.015498
TCGATAT200.002092832736.015499
TGCGCCC309.902513E-636.015498
TGCGCAG650.036.0154911
GATATGA200.002092832736.0154911
AAACACC251.4356103E-436.015499
GGACCGT309.902513E-636.015498
GGACTAG200.002092832736.015498
CACCGGC251.4356103E-436.0154912
Produced by FastQC (version 0.11.5)