FastQCFastQC Report
Thu 26 May 2016
SRR2099068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185418
Sequences flagged as poor quality0
Sequence length47
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA150.00514899956.607729
CGGCCGC150.00514899956.607730
TGACGGG1450.050.2117165
CTGCCAA2700.050.2117164
CTGTCGA252.026179E-550.211714
TGCCCTG301.0109597E-650.211715
TGCCCGG204.0844412E-450.211715
TGCCCCA204.0844412E-450.211715
TGCCCAG402.5538611E-950.211715
TGCCATA252.026179E-550.211715
CCGATTG204.0844412E-450.211714
TGCCACG150.0082753950.211715
TGCCAAT252.026179E-550.211715
TGCCAAA1550.050.211715
CTGAGGC150.0082753950.211714
TGACGTT700.050.211715
TGACGGA2750.050.211715
CTGACGC3050.050.211714
TGACGCA1600.050.211715
CTGAACG252.026179E-550.211714
Produced by FastQC (version 0.11.5)