##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099068_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 185418 Sequences flagged as poor quality 0 Sequence length 47 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.55348455921216 30.0 17.0 36.0 12.0 38.0 2 22.506568941526712 24.0 17.0 30.0 10.0 34.0 3 25.62271192656592 27.0 18.0 32.0 10.0 35.0 4 29.7443344227637 31.0 27.0 34.0 18.0 37.0 5 25.24512722605141 30.0 15.0 36.0 2.0 39.0 6 27.24430745666548 30.0 17.0 37.0 10.0 39.0 7 20.936182031949432 21.0 11.0 30.0 8.0 34.0 8 23.476641965720695 30.0 11.0 34.0 2.0 37.0 9 30.528999342027202 32.0 28.0 35.0 23.0 35.0 10 30.57851988480083 32.0 29.0 35.0 23.0 35.0 11 30.67023697807117 32.0 29.0 35.0 23.0 35.0 12 30.17865040071622 32.0 28.0 35.0 22.0 35.0 13 30.827363039187134 32.0 28.0 35.0 22.0 38.0 14 30.196679933986992 32.0 28.0 35.0 18.0 37.0 15 30.21162993884089 32.0 27.0 35.0 17.0 37.0 16 29.78787388495184 32.0 27.0 35.0 17.0 37.0 17 29.70037428944331 31.0 27.0 35.0 17.0 37.0 18 30.892879871425645 31.0 31.0 31.0 30.0 34.0 19 30.951417877444477 31.0 31.0 33.0 28.0 34.0 20 31.209057373070575 31.0 31.0 34.0 30.0 34.0 21 35.00186605399691 35.0 35.0 37.0 32.0 37.0 22 35.115630629173005 35.0 35.0 37.0 33.0 37.0 23 34.93603641501904 35.0 35.0 37.0 32.0 37.0 24 34.71459620964524 35.0 35.0 37.0 32.0 37.0 25 34.74276499584722 35.0 35.0 37.0 32.0 37.0 26 36.22198492055787 37.0 35.0 39.0 32.0 39.0 27 35.574960359835615 37.0 35.0 39.0 30.0 39.0 28 35.86985621676428 37.0 35.0 39.0 30.0 39.0 29 35.9881079506844 37.0 35.0 39.0 30.0 39.0 30 22.513628666041054 32.0 2.0 37.0 2.0 39.0 31 29.09995253966713 33.0 17.0 38.0 17.0 40.0 32 33.68421081016945 34.0 32.0 39.0 27.0 40.0 33 35.92370751491225 37.0 34.0 39.0 31.0 40.0 34 36.657945830501895 38.0 36.0 39.0 32.0 40.0 35 37.12880087154429 38.0 36.0 40.0 32.0 41.0 36 37.45386100594333 39.0 36.0 40.0 32.0 41.0 37 37.61187155508095 39.0 37.0 40.0 32.0 41.0 38 37.6514523940502 39.0 37.0 40.0 33.0 41.0 39 37.615404113947946 39.0 37.0 40.0 33.0 41.0 40 37.47622129458844 39.0 36.0 40.0 32.0 41.0 41 37.38845743131735 39.0 36.0 40.0 32.0 41.0 42 37.064335717136416 39.0 36.0 40.0 32.0 41.0 43 36.494930373534395 38.0 35.0 40.0 31.0 41.0 44 35.904291924192904 38.0 35.0 40.0 29.0 41.0 45 35.27206096495486 37.0 33.0 40.0 27.0 41.0 46 34.51186508321738 36.0 33.0 40.0 26.0 41.0 47 33.946914539041515 35.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.368370122166532 1101 2 2.4790654731479194 1101 3 1.6842020205222603 1101 4 3.4618856281248114 1101 5 8.695248115098693 1101 6 6.287011983622996 1101 7 5.563355089689484 1101 8 12.38002913460485 1101 9 2.5385251601253422 1101 10 3.1121533747183925 1101 11 3.215279111718189 1101 12 3.07996605330154 1101 13 4.273515564424745 1101 14 4.445970085955345 1101 15 3.7385847117954754 1101 16 3.9508259051014996 1101 17 3.4170184360880462 1101 18 0.219073666559769 1101 19 -0.3153410441258373 1101 20 -0.021160707411187474 1101 21 0.016273167795773702 1101 22 -0.07951171792118572 1101 23 -0.12839163497270079 1101 24 -0.2005606384195744 1101 25 -0.04037544881512645 1101 26 -0.1524969363210218 1101 27 1.21854058528546 1101 28 0.6489954970473093 1101 29 0.1638915922369435 1101 30 16.29944805552307 1101 31 9.2678115558486 1101 32 3.6410893172000343 1101 33 1.1915404255084283 1101 34 0.3409900161946098 1101 35 -0.10656529788653302 1101 36 -0.25645361169085845 1101 37 -0.5218817515974123 1101 38 -0.30687776218378815 1101 39 -0.15281113774969413 1101 40 -0.5846218832582437 1101 41 -0.6622139040700361 1101 42 -0.7071187421502358 1101 43 -1.0605529875900288 1101 44 -1.0310752069199225 1101 45 -1.188841149712374 1101 46 -0.9641485214050789 1101 47 -0.7971165420556261 1102 1 4.910377076551438 1102 2 1.4604246117920106 1102 3 1.6619333544318238 1102 4 1.0160711403818752 1102 5 7.607241761333615 1102 6 2.691701391524653 1102 7 -0.21808911135656928 1102 8 5.640008798897998 1102 9 2.082059884030773 1102 10 2.0910255814169787 1102 11 2.545016957932077 1102 12 0.9980918145566839 1102 13 3.1867290481874306 1102 14 3.4191787830523985 1102 15 2.6000384861727888 1102 16 3.7312179543584243 1102 17 2.7999071735516345 1102 18 0.34537486978116405 1102 19 0.2042448025880539 1102 20 0.030070944339069428 1102 21 0.10745975781481576 1102 22 -0.147478101503836 1102 23 -0.09504229527340158 1102 24 -0.12049953983407846 1102 25 -0.19800492126055502 1102 26 -0.29346180006111666 1102 27 0.9680836416476808 1102 28 0.37445369832812503 1102 29 0.11693592682182441 1102 30 16.143331718206436 1102 31 9.26992287173957 1102 32 3.6267521865469305 1102 33 1.337895623537662 1102 34 0.5445156850701593 1102 35 -0.17556371096967638 1102 36 -0.09958451784934397 1102 37 -0.32175156648759895 1102 38 -0.3510092948771728 1102 39 -0.5723419530529981 1102 40 -0.50504920749691 1102 41 -0.7680684229720356 1102 42 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9.423315851107905 1103 32 4.072427034172499 1103 33 1.3579107251749463 1103 34 0.6442082200809693 1103 35 0.2681881224125462 1103 36 0.1531261717196628 1103 37 -0.03012699970902588 1103 38 -0.1011967072822415 1103 39 -0.013013704299702056 1103 40 -0.1080961529463238 1103 41 -0.06259924009011542 1103 42 -0.29819650460884617 1103 43 -0.43860886720492687 1103 44 -0.48214224596024025 1103 45 -0.20588019002535418 1103 46 0.3078863355963719 1103 47 0.018546129843130643 1106 1 -4.641523208040947 1106 2 -0.14053363598348412 1106 3 -1.4889078141981713 1106 4 -4.9129824998789395 1106 5 -19.120342085346742 1106 6 -11.426573762479453 1106 7 -5.023399100909508 1106 8 -16.77409113265573 1106 9 -7.407928948419961 1106 10 -3.207738783293564 1106 11 -1.3834314732073736 1106 12 -3.655281866051407 1106 13 -0.5176695252482126 1106 14 -0.9359781030025758 1106 15 -2.8615559516671922 1106 16 -1.8566058145093294 1106 17 -6.581223270628634 1106 18 1.0219338236339688 1106 19 0.9277723071811579 1106 20 0.8582203733530811 1106 21 0.7410416591801408 1106 22 0.36172612055027287 1106 23 0.17970823608579423 1106 24 0.19390659871557858 1106 25 -0.05350403866391673 1106 26 0.04619020469409918 1106 27 1.4701406321732762 1106 28 0.6112273573216029 1106 29 0.8814159152940348 1106 30 16.844307978158167 1106 31 9.612413595468812 1106 32 3.30471609014576 1106 33 1.5110574809532764 1106 34 0.9159988076610261 1106 35 0.603902408126828 1106 36 0.24517574008501697 1106 37 0.1868042172221891 1106 38 0.15034882321065624 1106 39 0.516184290687768 1106 40 -0.09115101231697054 1106 41 -0.3574196243841854 1106 42 -0.9284527006545105 1106 43 0.032847551619909154 1106 44 -0.386180118419162 1106 45 -0.20954212263465877 1106 46 0.871837881126865 1106 47 -0.044402909421698666 1107 1 -2.354681102777789 1107 2 -1.875827753630542 1107 3 1.278971442767773 1107 4 0.410392113124157 1107 5 -19.120342085346742 1107 6 -8.067750233067688 1107 7 -1.4449935281540895 1107 8 -16.77409113265573 1107 9 -0.3477122301537072 1107 10 0.5551869133008616 1107 11 0.35410722648302695 1107 12 -3.268284962026641 1107 13 -26.180827419985054 1107 14 -25.57808336616047 1107 15 -11.119528087890103 1107 16 -5.744221913580539 1107 17 -1.1381892149010788 1107 18 0.8173672601664741 1107 19 0.7925555889149045 1107 20 0.5302017975016895 1107 21 0.7740911947838569 1107 22 0.41629268401776187 1107 23 0.23203021750994424 1107 24 0.4440613974771921 1107 25 0.6720377570017178 1107 26 0.7031561489665421 1107 27 2.0933604464147635 1107 28 1.1729146638231498 1107 29 1.1119886707119875 1107 30 17.095701167012653 1107 31 10.43276963262051 1107 32 4.8397006102696025 1107 33 2.44844138188207 1107 34 2.0050080955867244 1107 35 1.340667114009186 1107 36 1.032250043490592 1107 37 0.8118042172221891 1107 38 0.4424540863685493 1107 39 0.8654876962605229 1107 40 0.721774684277456 1107 41 0.33329244991922025 1107 42 0.9678321290668492 1107 43 0.851346003632294 1107 44 1.0594855162557621 1107 45 1.3630584965603916 1107 46 1.024856456978256 1107 47 1.0361698459962554 1201 1 7.114534052837907 1201 2 3.715127166903301 1201 3 2.530639701410877 1201 4 3.9912313500367844 1201 5 9.824177535762864 1201 6 7.351775357953564 1201 7 8.332610679970298 1201 8 13.796950815923427 1201 9 3.9160318331008206 1201 10 4.501726354517135 1201 11 4.140334640317306 1201 12 3.7744596053537656 1201 13 5.2106926521845445 1201 14 5.206309958151664 1201 15 4.808073466940218 1201 16 4.703985786278835 1201 17 4.649852388749856 1201 18 0.3007078900584865 1201 19 0.4340325450559561 1201 20 0.15484691942055306 1201 21 0.10162376396849737 1201 22 -0.04415665133207369 1201 23 0.014606035387835448 1201 24 0.0023675345463587405 1201 25 -0.11509509343209601 1201 26 0.028105073055805008 1201 27 1.4091953053921884 1201 28 0.7590041063660706 1201 29 0.26440383163427583 1201 30 0.10512036094191757 1201 31 -0.012907488024769265 1201 32 -0.04535869085274413 1201 33 -0.23444022813676924 1201 34 -0.26136272879173816 1201 35 -0.18617427207163217 1201 36 -0.21548470670177267 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0.603731209266396 2209 21 0.2667382536073859 2209 22 0.1883515075471749 2209 23 0.49673609986288625 2209 24 0.4440613974771921 2209 25 -0.10737400770416627 2209 26 0.9384502666136072 2209 27 1.8286545640618215 2209 28 1.4155617226466788 2209 29 0.4869886707119875 2209 30 -2.4925341271049923 2209 31 -1.0451715438500777 2209 32 0.07499472791666051 2209 33 1.5587354995291278 2209 34 1.8138316249984925 2209 35 1.098020055185657 2209 36 0.5101912199611789 2209 37 0.48092186428101513 2209 38 0.8983364393097233 2209 39 -0.950688774327709 2209 40 1.008539390159811 2209 41 0.9509395087427492 2209 42 -0.12040316505079574 2209 43 1.785169533044062 2209 44 1.633014928020465 2209 45 1.7012937906780365 2209 46 1.385150574625321 2209 47 0.4552874930550814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 9.0 25 127.0 26 839.0 27 3424.0 28 9491.0 29 19153.0 30 27100.0 31 28000.0 32 24249.0 33 22668.0 34 20222.0 35 16206.0 36 9905.0 37 3808.0 38 215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 98.19596802899395 1.040891391342804 0.283683353288246 0.4794572263750014 2 0.7070510953629098 89.64555760497902 2.4053759613414014 7.242015338316668 3 0.14238099860854933 2.5855094974597934 96.36712724762428 0.9049822563073703 4 0.0463816889406638 1.7592682479586663 0.2065603123752818 97.98778975072538 5 0.32740705963750477 4.6152040425505625 94.23763575037991 0.8197531474320168 6 97.09267342763684 1.8368396293500497 0.3320314636734605 0.7384554793396567 7 0.8995726741842224 86.47703843628699 1.261244658427303 11.362144231101487 8 0.8471074380165289 3.7734159779614327 0.715564738292011 94.66391184573003 9 21.850139957823885 46.55283070766343 15.8974829165161 15.69954641799658 10 21.9236217070543 46.84473320098658 15.526679901341515 15.704965190617601 11 23.505269175592446 44.21792921938539 15.606359684604515 16.67044192041765 12 21.16784778176876 45.238865698044414 16.07341250579771 17.51987401438911 13 21.509833749864175 44.394219276322936 15.154840812778442 18.941106161034448 14 21.1408526058587 45.753793373523884 15.600403744008648 17.50495027660877 15 20.055334731982505 46.94607290593635 16.203476488132157 16.79511587394899 16 21.054617643680988 42.789016313650855 19.00160238005392 17.15476366261423 17 19.937654380912317 47.826532483361916 15.583168840134185 16.652644295591582 18 23.431810948869675 28.45044743755023 25.98697873120843 22.130762882371663 19 28.967395701302557 29.801245921091663 17.974164666540815 23.257193711064968 20 28.74855731374516 30.014345964253742 21.479036555242747 19.75806016675835 21 21.100432536215468 34.695660615474225 24.861124594160223 19.34278225415008 22 18.314895820410893 27.91193143510553 29.792180193202846 23.98099255128074 23 20.29846077511353 27.838721159757952 18.998155518881664 32.86466254624686 24 24.13250062022026 26.243406788984885 29.587202968428095 20.03688962236676 25 23.987962333754005 33.04964998004509 20.70888479004196 22.25350289615895 26 14.52286185807203 35.092601581292 25.677118726337252 24.70741783429872 27 20.54729046719114 24.44183184634993 26.221816903265506 28.789060783193428 28 15.47961902296433 31.141528869904754 36.4468390339665 16.932013073164416 29 13.26785964685198 28.456244809026092 37.87819952755396 20.39769601656797 30 10.679282969683634 30.261353826362203 39.40281327758688 19.656549926367283 31 12.923442008467493 28.710729984089745 40.9120081978265 17.453819809616267 32 11.71851404409544 29.447296889157354 42.42729861500625 16.40689045174095 33 11.012415191621095 34.49665081060091 39.19576308664747 15.295170911130526 34 13.961967015068655 34.44757251183812 40.32564260212062 11.264817870972612 35 12.094834374224725 37.24935011703286 39.04259564875039 11.613219859992018 36 10.172151571044882 40.576427315578854 40.14874499778878 9.102676115587483 37 11.264278549008187 39.74209623661133 39.10246038680171 9.891164827578768 38 9.588065883571174 41.90963121164072 39.90928604558349 8.593016859204608 39 8.607578552244119 41.74675597838397 40.57157341789902 9.074092051472888 40 7.870864748837761 41.81471054590169 39.89634231843726 10.418082386823285 41 8.275356222157503 42.383695218371464 39.99072366221187 9.350224897259166 42 9.337281170112934 42.254797268873574 40.0344087413304 8.373512819683095 43 10.07992751512798 43.15222901767897 38.39756657929651 8.370276887896537 44 10.011433625645838 43.172723252327174 37.46561822476782 9.350224897259166 45 9.404696415666224 43.68561844049661 37.71802090411934 9.191664239717827 46 9.432741157816393 43.44184491257591 38.031366965451035 9.094046964156663 47 9.764963487903008 42.562750110561005 37.17546300790646 10.496823393629528 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 2.0 7 23.0 8 44.0 9 44.0 10 101.5 11 159.0 12 312.0 13 465.0 14 837.5 15 1210.0 16 1732.0 17 2254.0 18 3104.5 19 3955.0 20 5556.0 21 7157.0 22 9132.0 23 11107.0 24 12692.0 25 14277.0 26 14277.0 27 14838.5 28 15400.0 29 14804.0 30 14208.0 31 13540.0 32 12872.0 33 12724.5 34 12577.0 35 13111.5 36 13646.0 37 14069.0 38 14492.0 39 14203.0 40 13914.0 41 13224.5 42 12535.0 43 12535.0 44 11823.0 45 11111.0 46 9875.5 47 8640.0 48 7340.5 49 6041.0 50 4778.0 51 3515.0 52 2884.5 53 2254.0 54 1865.5 55 1477.0 56 1123.5 57 770.0 58 770.0 59 613.0 60 456.0 61 376.5 62 297.0 63 252.0 64 207.0 65 150.5 66 94.0 67 85.0 68 76.0 69 85.5 70 95.0 71 76.5 72 58.0 73 44.0 74 30.0 75 30.0 76 20.5 77 11.0 78 10.0 79 9.0 80 5.5 81 2.0 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 12.69563904259565 6 1.4043943953661457 7 6.353212740942088 8 21.690450765297868 9 0.0026966098221316162 10 1.383360838753519 11 0.0 12 0.0 13 0.732399227690947 14 2.21930988361432 15 5.393219644263232E-4 16 1.383360838753519 17 0.0 18 0.015640336968363373 19 0.007011185537542201 20 0.0 21 0.0 22 0.009168473395247496 23 0.0 24 0.0 25 0.0 26 0.0 27 1.8304587472629412 28 0.0 29 0.0 30 36.276952615172206 31 0.0016179658932789695 32 0.0010786439288526465 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 47 185418.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.53792312680514 #Duplication Level Percentage of deduplicated Percentage of total 1 84.1895436312081 60.227451003701724 2 9.533387869584832 13.639975371047527 3 2.757322158757297 5.917593018870477 4 1.1513226251033128 3.294529177951691 5 0.650156924792572 2.3255438053085524 6 0.3961909505119399 1.700560665675541 7 0.26229899721924116 1.3135027849515706 8 0.17812734191203233 1.0194288073988085 9 0.14842945762867027 0.9556501616633875 >10 0.7144195622341708 8.710313403192343 >50 0.016821483042799824 0.745383988656934 >100 0.001978998005035274 0.150067811581453 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 5.393219644263232E-4 0.0 0.0 15 0.0 0.0 5.393219644263232E-4 0.0 0.0 16 0.0 0.0 0.002157287857705293 0.0 0.0 17 0.0 0.0 0.022112200541479252 0.0 0.0 18 0.0 0.0 0.08089829466394849 0.0 0.0 19 0.0 0.0 0.22813319095233472 0.0 0.0 20 0.0 0.0 0.4012555415331845 0.0 0.0 21 0.0 0.0 0.4843111240548383 0.0 0.0 22 0.0 0.0 0.5220636615646809 0.0 0.0 23 0.0 0.0 0.5862429753314133 0.0 0.0 24 0.0 0.0 0.6504222890981458 0.0 0.0 25 0.0 0.0 0.7221521103668468 0.0 0.0 26 0.0 0.0 0.782556170382595 0.0 0.0 27 0.0 0.0 0.8672297187975277 0.0 0.0 28 0.0 0.0 0.9691615700741029 0.0 0.0 29 0.0 0.0 1.1255649397577365 0.0 0.0 30 0.0 0.0 1.228036112998738 0.0 0.0 31 0.0 0.0 1.4761242166348467 0.0 0.0 32 0.0 0.0 1.6896957145476708 0.0 0.0 33 0.0 0.0 1.845559762266878 0.0 0.0 34 0.0 0.0 2.026771942314123 0.0 0.0 35 0.0 0.0 2.1918044634285776 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGTA 15 0.005148999 56.6077 29 CGGCCGC 15 0.005148999 56.6077 30 TGACGGG 145 0.0 50.211716 5 CTGCCAA 270 0.0 50.211716 4 CTGTCGA 25 2.026179E-5 50.21171 4 TGCCCTG 30 1.0109597E-6 50.21171 5 TGCCCGG 20 4.0844412E-4 50.21171 5 TGCCCCA 20 4.0844412E-4 50.21171 5 TGCCCAG 40 2.5538611E-9 50.21171 5 TGCCATA 25 2.026179E-5 50.21171 5 CCGATTG 20 4.0844412E-4 50.21171 4 TGCCACG 15 0.00827539 50.21171 5 TGCCAAT 25 2.026179E-5 50.21171 5 TGCCAAA 155 0.0 50.21171 5 CTGAGGC 15 0.00827539 50.21171 4 TGACGTT 70 0.0 50.21171 5 TGACGGA 275 0.0 50.21171 5 CTGACGC 305 0.0 50.21171 4 TGACGCA 160 0.0 50.21171 5 CTGAACG 25 2.026179E-5 50.21171 4 >>END_MODULE