FastQCFastQC Report
Thu 26 May 2016
SRR2099067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386481
Sequences flagged as poor quality0
Sequence length47
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGGCACTCTTGGTCCGACGATCGTCGGACTGTAGAACTCTG11360.2939342425630238Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGAAACTCAGGGTCCGACGATCGTCGGACTGTAGAACTCTG10070.2605561463564833Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGACTGATAGAGTACCGTCAACAGTCTAGGTACGTACTCTATTGA6500.168184205691871No Hit
CTATAGTGGCACTCTTGGTCCGACGATCGNCGGACTGTAGAACTCTG5860.15162453005451756Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTATAGTGAAACTCAGGGTCCGACGATCGNCGGACTGTAGAACTCTG5690.14722586621334555Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTATAGTGTAACACTAACAGTCCGACGATCGTCGGACTGTAGAACTC5190.13428861962166316Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTAGACTGACCGACTCTGTTGACGATCGTCGGACTGTAGAACTCTGA5130.13273615003066128Illumina DpnII expression Sequencing Primer (100% over 24bp)
CTAGACTGAACAGTTACAACAGTCTAGGTACGTAACTGTTTGACGAT4380.11333028014313769No Hit
CTATAGTGCAAACTAGAGTCCGACGATCGTCGGACTGTAGAACTCTG4180.10815538150646474Illumina DpnII expression Sequencing Primer (100% over 23bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCT150.004005044260.33478525
TCAACGT150.004565719558.37164728
CACGCAT258.327164E-658.37164329
AGTGTCG302.0121763E-550.2899727
ACTGCGA3450.050.2680175
ACTGCCA2700.050.2680135
GACCGGA150.00824808950.2680134
CCTGTGG252.018449E-550.2680135
CCTGTAT150.00824808950.2680135
GCCTGGA750.050.2680134
ACTACAA150.00824808950.2680135
CCTGCGG402.5465852E-950.2680135
CCTGCGC150.00824808950.2680135
GACTATT204.0695316E-450.2680134
GACTAGG301.0071253E-650.2680134
ACTGCGT1700.050.268015
ACTGAGC3850.050.268015
AGTGGGG850.050.268015
GACTGCT10800.050.0352944
GACTGAG15600.049.7846644
Produced by FastQC (version 0.11.5)