##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	386481
Sequences flagged as poor quality	0
Sequence length	47
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11471197807913	34.0	27.0	37.0	17.0	39.0
2	30.35578204361922	32.0	27.0	38.0	18.0	39.0
3	24.59439661975621	27.0	18.0	32.0	10.0	35.0
4	28.183434631974148	30.0	25.0	34.0	16.0	37.0
5	21.34486559494516	25.0	10.0	32.0	2.0	36.0
6	27.817018171656564	31.0	18.0	34.0	16.0	37.0
7	26.692709344055725	30.0	18.0	36.0	8.0	39.0
8	24.107687053179845	31.0	9.0	37.0	2.0	39.0
9	31.456860751240036	33.0	30.0	35.0	25.0	37.0
10	31.091261924907045	33.0	30.0	35.0	25.0	37.0
11	31.158941836726772	32.0	30.0	35.0	24.0	37.0
12	30.429102594952923	32.0	28.0	35.0	22.0	36.0
13	31.065721212685748	32.0	28.0	37.0	23.0	39.0
14	30.776537527071188	32.0	28.0	37.0	19.0	39.0
15	30.955834827585313	32.0	28.0	37.0	18.0	39.0
16	30.49116774175186	32.0	27.0	37.0	17.0	39.0
17	30.270052085354777	32.0	27.0	35.0	17.0	39.0
18	30.841772299285086	31.0	31.0	31.0	28.0	34.0
19	30.910194809059178	31.0	31.0	33.0	28.0	34.0
20	31.148237558896817	31.0	31.0	34.0	28.0	34.0
21	34.93893102118862	35.0	35.0	37.0	32.0	37.0
22	35.064681058059776	35.0	35.0	37.0	33.0	37.0
23	34.93774079450219	35.0	35.0	37.0	32.0	37.0
24	34.84038542645046	35.0	35.0	37.0	32.0	37.0
25	34.84855400394845	35.0	35.0	37.0	32.0	37.0
26	36.43261117622858	37.0	35.0	39.0	32.0	39.0
27	35.94059475110031	37.0	35.0	39.0	32.0	39.0
28	36.339871817760766	37.0	35.0	39.0	32.0	39.0
29	36.5751330595812	37.0	35.0	39.0	32.0	39.0
30	22.04740983385988	32.0	2.0	37.0	2.0	39.0
31	29.214243391007578	34.0	17.0	39.0	17.0	40.0
32	34.41036428698953	36.0	32.0	39.0	27.0	40.0
33	36.505088219084506	37.0	35.0	39.0	32.0	40.0
34	37.2789736106044	38.0	36.0	40.0	33.0	40.0
35	37.72470056742763	39.0	37.0	40.0	33.0	41.0
36	37.99516405722403	39.0	37.0	40.0	34.0	41.0
37	38.048289566628114	39.0	37.0	40.0	34.0	41.0
38	37.95780646396589	39.0	37.0	40.0	34.0	41.0
39	37.87947402330257	39.0	37.0	40.0	33.0	41.0
40	37.704976441273956	39.0	37.0	40.0	33.0	41.0
41	37.643648717530745	39.0	37.0	40.0	33.0	41.0
42	37.457574887251894	39.0	36.0	40.0	32.0	41.0
43	37.151148439379945	39.0	36.0	40.0	31.0	41.0
44	36.924984669362786	39.0	36.0	40.0	31.0	41.0
45	36.665675673577745	39.0	35.0	40.0	31.0	41.0
46	36.36517189719547	38.0	35.0	40.0	30.0	41.0
47	36.166383340966306	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.94940225696531
1101	2	4.545682898155821
1101	3	2.5621682095754643
1101	4	3.4634032734401607
1101	5	7.706071241067001
1101	6	6.067629321507486
1101	7	7.8835142037223775
1101	8	13.765042748750691
1101	9	2.986545774563222
1101	10	3.3627786423434145
1101	11	3.3346020393941025
1101	12	3.4984859366721572
1101	13	4.510474749710827
1101	14	5.104028280328958
1101	15	4.403121869880501
1101	16	4.666251741888562
1101	17	3.885958214739677
1101	18	0.20028497282008928
1101	19	-0.5313737131864009
1101	20	-0.049896123275885884
1101	21	0.12011481896702492
1101	22	-0.08661250827561418
1101	23	-0.06970623811885446
1101	24	0.017653286273656477
1101	25	0.043713010356718485
1101	26	0.020019666210572495
1101	27	1.331990959924397
1101	28	0.7177329006367756
1101	29	0.4530307444584878
1101	30	17.1444435200099
1101	31	10.097987172269058
1101	32	4.075844328261489
1101	33	1.8140243430634158
1101	34	0.9560958581737893
1101	35	0.3948496151797656
1101	36	-0.005084367757589803
1101	37	-0.18996179760493703
1101	38	-0.08321239203711173
1101	39	0.01806734167918478
1101	40	-0.10375755177062729
1101	41	-0.33791133787565286
1101	42	-0.18959152356871556
1101	43	-0.33100697087510866
1101	44	-0.42978049088196
1101	45	-0.5190650597062572
1101	46	-0.33922303422872346
1101	47	-0.2575886203459561
1102	1	6.341667561561724
1102	2	2.607109401110513
1102	3	2.2150460588347407
1102	4	0.7318183869399135
1102	5	7.979162719081604
1102	6	5.191190083133165
1102	7	3.8789541861945054
1102	8	9.907921554931253
1102	9	2.995767253733451
1102	10	2.4919172615426035
1102	11	2.985585393669897
1102	12	1.4214956057604091
1102	13	3.804244355485398
1102	14	4.334065359721524
1102	15	3.7631943553606604
1102	16	4.717651487650787
1102	17	3.323676496067211
1102	18	0.4794236181646667
1102	19	0.35560264039967393
1102	20	0.20673473989325686
1102	21	0.21700675954587467
1102	22	0.09439019881810395
1102	23	-0.004076863668231567
1102	24	-0.09210218634872547
1102	25	-0.05227926734079347
1102	26	-0.05395557828804698
1102	27	1.3131971304456798
1102	28	0.712693899558019
1102	29	0.39316041513637856
1102	30	17.021635745625794
1102	31	10.095550944797893
1102	32	3.995092198588715
1102	33	1.725091320755645
1102	34	0.904062871057711
1102	35	0.30138141346493796
1102	36	-0.03804978315817209
1102	37	-0.1122430561185297
1102	38	-0.18820443599483383
1102	39	-0.24106615825257904
1102	40	-0.2493120771977999
1102	41	-0.5316337773660678
1102	42	-0.42711167512831594
1102	43	-0.4452194481874443
1102	44	-0.42395712809322816
1102	45	-0.7829366272740472
1102	46	-0.5655817186554302
1102	47	-0.4500050975892691
1103	1	1.1473766572330248
1103	2	0.7152434145362427
1103	3	0.5582511184331409
1103	4	-3.6651748234258505
1103	5	2.982783894973096
1103	6	-8.33703081593453
1103	7	-1.1863788265109392
1103	8	-17.185853070758267
1103	9	-1.1393102275764235
1103	10	-2.8266446094791853
1103	11	-1.6735724839541177
1103	12	-0.8941879525769316
1103	13	-0.41662761689544325
1103	14	-0.5391906616476447
1103	15	-2.2286443101047837
1103	16	-1.5773831830621141
1103	17	-1.523572539226695
1103	18	-0.49817363707996876
1103	19	0.21643546005100234
1103	20	0.23238779342230842
1103	21	0.17104746122420522
1103	22	0.15014445334696802
1103	23	0.14503091020330316
1103	24	0.12149074267735926
1103	25	0.19021589501659264
1103	26	0.37424578957374877
1103	27	1.7288482873027178
1103	28	1.0349599258720872
1103	29	0.6947549743972203
1103	30	17.32434403521149
1103	31	10.482089315150198
1103	32	4.414225103364494
1103	33	2.1194337061819795
1103	34	1.0458932950316466
1103	35	0.5577595376111546
1103	36	0.387561159736201
1103	37	0.05237339841440303
1103	38	0.1504943341036764
1103	39	0.08163395757988923
1103	40	-0.04833709746284143
1103	41	-0.22086237376103668
1103	42	-0.21347132930178248
1103	43	-0.28367040905503416
1103	44	-0.19767343111819713
1103	45	-0.1877739311999349
1103	46	0.10179845796086084
1103	47	0.012760859857536389
1106	1	-6.825210525658864
1106	2	-4.383526590355785
1106	3	-2.8252487767377303
1106	4	-4.510471489859565
1106	5	-15.969221835685929
1106	6	-10.48231699945539
1106	7	-4.313805620836188
1106	8	-17.185853070758267
1106	9	-6.690749880941439
1106	10	-3.000094885529222
1106	11	-1.6998496521699238
1106	12	-5.667320246937123
1106	13	-1.6367827640056518
1106	14	-1.924871002691745
1106	15	-3.1801852982940346
1106	16	-2.649278493705765
1106	17	-6.433831816604567
1106	18	0.7632038176687921
1106	19	0.7890820461157162
1106	20	0.2770205420420595
1106	21	0.26178755487155314
1106	22	-0.0567043830497056
1106	23	0.3282512610314541
1106	24	0.2578699445782604
1106	25	0.4387238270873155
1106	26	0.5347007479915291
1106	27	1.510663927808693
1106	28	1.1830066097773155
1106	29	0.8629637940212334
1106	30	17.55374860576149
1106	31	10.439493745928026
1106	32	4.373369697675763
1106	33	1.9106455309076722
1106	34	1.1933669130343034
1106	35	0.7838969336556048
1106	36	0.11754229049306275
1106	37	0.2554134431414994
1106	38	-0.0753250802509271
1106	39	-0.34988816136622347
1106	40	-0.04347302589948754
1106	41	-0.5213760364907856
1106	42	-0.5322741806540776
1106	43	-0.815755110990878
1106	44	-0.4620839419854832
1106	45	-0.19706528629113507
1106	46	0.08851311307302723
1106	47	-0.051873286483925085
1107	1	-3.578404486169898
1107	2	-0.6515869852454479
1107	3	0.29057848543099496
1107	4	1.3126622869327491
1107	5	-15.969221835685929
1107	6	-8.389444018777532
1107	7	-2.765141277049892
1107	8	-17.185853070758267
1107	9	-1.1382527845419048
1107	10	-0.014919085719277803
1107	11	-0.3843602635098051
1107	12	-4.817515580059286
1107	13	-26.568490081078824
1107	14	-26.319992953911257
1107	15	-11.802411040031973
1107	16	-7.128689326775135
1107	17	-2.1559730899653538
1107	18	0.5052901865844284
1107	19	0.6813320725120846
1107	20	0.41860010069478903
1107	21	0.4022584660741728
1107	22	0.18352368156860877
1107	23	0.06858491112119935
1107	24	0.22146407402604495
1107	25	0.31655087808509563
1107	26	0.01592765116964756
1107	27	1.6626542139453164
1107	28	1.1654688629712808
1107	29	0.7354587787113402
1107	30	17.496025028525715
1107	31	10.500205392005526
1107	32	4.471395249359851
1107	33	2.0521511797303944
1107	34	1.1641408545399514
1107	35	0.7620724167091382
1107	36	0.25911129059864635
1107	37	0.3317306218977052
1107	38	0.30636639900153284
1107	39	0.052983763456921906
1107	40	-0.40266424118826194
1107	41	0.2159484276629442
1107	42	0.03540610653840304
1107	43	0.03261359347538928
1107	44	0.15879030571803554
1107	45	0.1601620392288723
1107	46	-0.057363774267273016
1107	47	-0.07923048213380213
1201	1	7.4688022092087
1201	2	5.735443773177227
1201	3	4.3689031968538465
1201	4	3.6502621469828966
1201	5	10.222353960764789
1201	6	7.552968283480823
1201	7	9.71260697427116
1201	8	14.428215776793582
1201	9	4.067816429545271
1201	10	4.484832392223346
1201	11	4.1622466467917825
1201	12	4.308826647309235
1201	13	5.413549688003922
1201	14	5.7405159192980655
1201	15	5.246311778735745
1201	16	5.461059068938621
1201	17	4.711321684962517
1201	18	0.2689929153288517
1201	19	0.3920065808132769
1201	20	0.14750141105857395
1201	21	0.21414809451287198
1201	22	0.04736691552088246
1201	23	-0.060473009790470655
1201	24	0.042244960874924686
1201	25	-0.03234215746605429
1201	26	0.05343226757099728
1201	27	1.4533361640243143
1201	28	0.661345340566811
1201	29	0.28587821174783556
1201	30	-0.07410539696070728
1201	31	-0.16576707897015197
1201	32	-0.2351932231041829
1201	33	-0.13419038121026716
1201	34	-0.040160937317963885
1201	35	-0.042301849178919326
1201	36	-0.14634064468009456
1201	37	-0.09775436820306282
1201	38	-0.0889308417094341
1201	39	0.012931448869643702
1201	40	0.07211701774950541
1201	41	-0.10585848041721135
1201	42	-0.146113571132652
1201	43	-0.11308558654066303
1201	44	-0.11344686485139732
1201	45	-0.16577255642990707
1201	46	-0.13299222962632484
1201	47	-0.26396567424551876
1202	1	5.603550564429224
1202	2	3.2443123131962253
1202	3	1.9486167748252186
1202	4	1.8483878607939168
1202	5	3.856397460272351
1202	6	2.8721587851981525
1202	7	5.809236322751435
1202	8	6.29133076235777
1202	9	2.507087750629136
1202	10	2.5673503397704884
1202	11	2.560127452164352
1202	12	2.735540121301497
1202	13	3.506803269638258
1202	14	3.8061478544329397
1202	15	3.085580911519301
1202	16	3.558420173893687
1202	17	2.3223198035817774
1202	18	0.2807364916917514
1202	19	0.4905003054091317
1202	20	0.2581729572168001
1202	21	0.3794394771534897
1202	22	0.08773387815168121
1202	23	0.03914260201787556
1202	24	0.038720264163593754
1202	25	0.07621070977882738
1202	26	0.011650854519970721
1202	27	1.4330730606353512
1202	28	0.7208019479653842
1202	29	0.5075101736440928
1202	30	9.434638674766482
1202	31	5.56461696912897
1202	32	2.2524376532836143
1202	33	1.0075037367778705
1202	34	0.5106277010267988
1202	35	0.25035955330135806
1202	36	0.048731275133889085
1202	37	0.005822067175593304
1202	38	-0.03029693766180941
1202	39	0.02217276654423017
1202	40	0.0745732106254593
1202	41	-0.0898277170962487
1202	42	-0.23604560559127208
1202	43	-0.21346236446498068
1202	44	-0.4204313201137637
1202	45	-0.4482704886951794
1202	46	-0.17560429081287054
1202	47	-0.16455335135498217
1203	1	3.818040393151694
1203	2	-1.7347572396485766
1203	3	-3.2724589337708068
1203	4	-1.4714805109086768
1203	5	2.6917370684236595
1203	6	0.16237060495489786
1203	7	1.1725625716128611
1203	8	6.221681175817075
1203	9	2.0801099029988634
1203	10	2.5394306741099335
1203	11	1.8311114793591976
1203	12	1.9698887963861864
1203	13	2.6906423390124985
1203	14	3.3843449456321224
1203	15	2.0732783390820657
1203	16	2.5325067830511117
1203	17	1.0444953913250465
1203	18	-1.283967359522432
1203	19	-1.0860432462330962
1203	20	-0.28166082559353356
1203	21	0.041868855684562334
1203	22	-0.1161709160711979
1203	23	-0.1285033887068252
1203	24	-0.0733974571368563
1203	25	-0.037849287958906075
1203	26	-0.06719812716160334
1203	27	1.360424480208735
1203	28	0.7724486411839351
1203	29	0.6360844087890243
1203	30	17.039404023959506
1203	31	10.074169099530877
1203	32	3.7982054254850937
1203	33	1.7368010047911824
1203	34	0.7871468439842957
1203	35	0.31944654942573436
1203	36	0.16308745142412562
1203	37	-0.2891405160967224
1203	38	-0.158853312793525
1203	39	0.08976547489616138
1203	40	-0.15115205474457127
1203	41	-0.12288511322538653
1203	42	-0.1985232061580291
1203	43	-0.29369252050196337
1203	44	-0.5720405073045569
1203	45	-0.5811058253266665
1203	46	-0.23968601823334978
1203	47	-0.014636400416435436
2101	1	5.438087766608557
2101	2	1.9426046266633712
2101	3	-0.5474326800077662
2101	4	0.6239781715024684
2101	5	3.1990916225764217
2101	6	3.1417491492129095
2101	7	3.900454569465886
2101	8	-1.1960745358349278
2101	9	1.9785233978220127
2101	10	2.8835299950125943
2101	11	2.313504733503404
2101	12	2.643927519714307
2101	13	3.157574654866661
2101	14	3.769274507758251
2101	15	2.3603307610403235
2101	16	2.6636312997874008
2101	17	1.8395799089948
2101	18	0.34542013046224085
2101	19	0.3032300496059719
2101	20	-0.8216124408806138
2101	21	-0.40086254692758416
2101	22	-0.029197838819015942
2101	23	0.12732883613428925
2101	24	0.19755499049175285
2101	25	0.30795555517972417
2101	26	0.46405629708364415
2101	27	1.7300930215885515
2101	28	1.0886699662405235
2101	29	0.6259504562558291
2101	30	-4.574559866420337
2101	31	-2.6935348824843217
2101	32	-0.9930324297185962
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2204	18	-0.017175853122992635
2204	19	-0.12636310587274835
2204	20	0.14971668525965143
2204	21	0.349340120052382
2204	22	0.28585238734558516
2204	23	0.23995377214523472
2204	24	0.2674037665174609
2204	25	0.3612217244800817
2204	26	0.4339378019384199
2204	27	1.8205882339482997
2204	28	1.0291798646133188
2204	29	0.5891682875932602
2204	30	-17.67064163814095
2204	31	-10.629291547836239
2204	32	-4.088128560163955
2204	33	-1.524912561126449
2204	34	-0.4116913592232976
2204	35	-0.1772468846791284
2204	36	0.22470316998363415
2204	37	0.36080212510713494
2204	38	0.4973650555174274
2204	39	0.37053115859052355
2204	40	0.7702720179548947
2204	41	0.6019388739982219
2204	42	0.6485200041350581
2204	43	0.8015343279133589
2204	44	0.29325067293702034
2204	45	0.31145327672700773
2204	46	0.42498433739118724
2204	47	0.3489677564092588
2205	1	-3.462096593149674
2205	2	-2.4448029017490356
2205	3	0.5658972306127552
2205	4	-0.18654900405936203
2205	5	7.100641178012701
2205	6	3.746617180297367
2205	7	5.593110516818339
2205	8	9.582640079926666
2205	9	0.19495008564726746
2205	10	1.4106635508222638
2205	11	1.3210658172587415
2205	12	1.507788339765181
2205	13	2.219181151797887
2205	14	2.4074043063627144
2205	15	1.7707669235569519
2205	16	2.4473469656995164
2205	17	1.6559109164392005
2205	18	-1.9357938435406972
2205	19	-1.8387829722604963
2205	20	-1.0725284055022115
2205	21	-3.272058084954704
2205	22	-1.0885453452949392
2205	23	-0.38558101427760505
2205	24	-0.20330613293594268
2205	25	-0.1609086486894995
2205	26	-0.1820383098100109
2205	27	1.1663605532680918
2205	28	0.7464212439236633
2205	29	0.2806049567342299
2205	30	-17.67064163814095
2205	31	-10.631538663026198
2205	32	-4.423255761729514
2205	33	-1.823701278313841
2205	34	-1.2966431103535996
2205	35	-0.8298685190674178
2205	36	-0.33120478601879455
2205	37	-0.044619968151515366
2205	38	-0.006798518272972842
2205	39	0.3224138767226492
2205	40	0.5586652968536043
2205	41	0.026201644765478704
2205	42	0.24965030982369996
2205	43	0.4382709498176709
2205	44	0.3594663420105064
2205	45	0.3239170057235654
2205	46	0.11373863930089811
2205	47	-0.47285557849863125
2206	1	-4.878215427916324
2206	2	-1.9219639201385128
2206	3	-2.1259447762535544
2206	4	-1.311016185132818
2206	5	-15.969221835685929
2206	6	-8.579756931671092
2206	7	-5.338885812084751
2206	8	-7.142180614182584
2206	9	-1.3285517329053675
2206	10	-0.26133073858254363
2206	11	-0.7668229621015357
2206	12	-1.0465370961775768
2206	13	-0.8325099321954497
2206	14	-0.5125487851767652
2206	15	-1.7549477506331996
2206	16	-0.301835535405214
2206	17	-1.0520125343918956
2206	18	0.6401026859130567
2206	19	0.7358592657489567
2206	20	0.43477639751624864
2206	21	0.4716041741295598
2206	22	0.11485041934695772
2206	23	0.16501213980649254
2206	24	0.17362374231474575
2206	25	0.29581999606389076
2206	26	0.3706385316869856
2206	27	1.752230229327786
2206	28	1.1000192588119972
2206	29	0.5033317659391656
2206	30	-4.664190025237724
2206	31	-2.6461686640459483
2206	32	-1.0063313251409127
2206	33	-0.256696961375809
2206	34	0.0458634330367218
2206	35	0.18862973036322472
2206	36	0.22396364254777268
2206	37	0.1895416280958102
2206	38	0.31858643702801714
2206	39	0.3009379597982118
2206	40	0.29639261593310806
2206	41	0.23542357617035492
2206	42	0.11395229153620079
2206	43	0.07713358960828742
2206	44	0.06791666323148604
2206	45	-0.2250989363546836
2206	46	0.03364414254709658
2206	47	-0.19902574063873857
2207	1	-10.527287356853716
2207	2	-9.186454960871718
2207	3	-2.889557334515249
2207	4	-1.1093540853205894
2207	5	-15.969221835685929
2207	6	-8.675404503790151
2207	7	-20.669903181811797
2207	8	-5.365947857488125
2207	9	-3.260911326631728
2207	10	-0.6973630240873874
2207	11	-4.203567912101338
2207	12	-1.2713737282302304
2207	13	-2.241475863069347
2207	14	-1.2520624641798186
2207	15	-2.7919373115487645
2207	16	-0.7988951083553246
2207	17	-0.02167137272524755
2207	18	0.9231622039825105
2207	19	1.0391109445089661
2207	20	0.70961792962731
2207	21	0.45695574352026824
2207	22	0.3534832638504639
2207	23	0.3406043608657683
2207	24	0.394254948238725
2207	25	0.3413246824797582
2207	26	0.39543361124432863
2207	27	1.9565854012414334
2207	28	1.3039251933707376
2207	29	0.9201903188931126
2207	30	12.882280952696334
2207	31	8.144344822874814
2207	32	3.516983152767658
2207	33	1.5536386338244839
2207	34	1.1790564286972298
2207	35	0.8297259322299482
2207	36	0.5957683144594625
2207	37	0.4500499013544257
2207	38	0.21566247417437978
2207	39	0.3042096307965281
2207	40	0.44513330092207326
2207	41	0.2739693956365201
2207	42	0.08295334602877347
2207	43	0.04950902032341986
2207	44	-0.08885541349166459
2207	45	0.09351383750957609
2207	46	0.45583866310578003
2207	47	0.2856676529065112
2208	1	-4.0800465222946904
2208	2	-0.8360661742223883
2208	3	-0.014885665610368193
2208	4	0.6998550541854875
2208	5	2.7968951058433085
2208	6	2.871367134087592
2208	7	0.02424974172643246
2208	8	8.883112446483114
2208	9	-3.5194550405567533
2208	10	-27.131802202602394
2208	11	-12.446842009649721
2208	12	-6.75786034274288
2208	13	-1.2416534993696793
2208	14	0.24372518701827772
2208	15	0.03800316844820273
2208	16	-25.96635166443747
2208	17	-12.329827721091348
2208	18	0.8139443010856198
2208	19	0.7984066521054061
2208	20	0.4815329199994238
2208	21	0.6514390705771191
2208	22	0.3135885192796195
2208	23	0.30881220007412935
2208	24	0.3316038093531901
2208	25	0.4380583061892267
2208	26	0.5067049897730769
2208	27	1.7028970795255134
2208	28	1.191848530280481
2208	29	0.7236724640764507
2208	30	-17.67064163814095
2208	31	-10.413506583175714
2208	32	-3.5107565318923797
2208	33	-1.1127793420217174
2208	34	-0.2916442399258017
2208	35	0.15558669852926243
2208	36	0.551432662380293
2208	37	0.5091743675573426
2208	38	0.7107940553032464
2208	39	0.8332105331889323
2208	40	0.8501606450699057
2208	41	0.8289146718135996
2208	42	0.9748746839380189
2208	43	0.9367881295839524
2208	44	0.9964526433803726
2208	45	1.0428732810105785
2208	46	0.9499896918568069
2208	47	1.2320262271738756
2209	1	-9.215454428097889
2209	2	-6.720111956209443
2209	3	-5.654517069420656
2209	4	-3.74461783132298
2209	5	-7.4326364698322696
2209	6	-6.370121877504172
2209	7	-1.499171474494723
2209	8	2.521464002412465
2209	9	-6.563920612648758
2209	10	-3.4976558611389805
2209	11	-4.968142335096754
2209	12	-4.447808051815173
2209	13	-3.0075144713227253
2209	14	-2.905358807569794
2209	15	-7.511892615367206
2209	16	-3.673668737608203
2209	17	-5.175295231238714
2209	18	0.8314965005956196
2209	19	0.6671308266035219
2209	20	0.3355571274079132
2209	21	-0.7528465914699041
2209	22	0.24085659256005698
2209	23	-0.5205292267734265
2209	24	0.26274799335874377
2209	25	0.49726041245317276
2209	26	0.14933489433299485
2209	27	1.657005391223322
2209	28	0.7537383170943954
2209	29	-0.7126459620763299
2209	30	0.30496811795661216
2209	31	-0.2678233272427022
2209	32	-0.10467908281204075
2209	33	-0.08447642031183733
2209	34	0.10068398620503416
2209	35	-0.31854209073463835
2209	36	0.08827399781013412
2209	37	-0.31044021539508293
2209	38	-0.09971532415336526
2209	39	-0.7742784052686673
2209	40	-0.3459120502897335
2209	41	-0.6140589633200548
2209	42	0.10187216080933581
2209	43	0.12083025486278132
2209	44	0.752550204355984
2209	45	0.3590322746844734
2209	46	0.5616838447803474
2209	47	1.006663298881925
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	7.0
25	152.0
26	1135.0
27	5335.0
28	14811.0
29	30221.0
30	46748.0
31	53877.0
32	46871.0
33	42174.0
34	43674.0
35	42980.0
36	34059.0
37	21232.0
38	3205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.07684724475459337	0.650743503561624	0.17335910432854398	99.09905014735524
2	0.06494497789024557	2.1923458074264968	97.02029336500372	0.7224158496795444
3	0.7762347955009431	93.70137212437352	1.1982477793216226	4.324145300803921
4	84.55448003912224	1.2577591136433615	13.997842067268508	0.1899187799658974
5	0.6620994773060119	90.03541818582906	1.6765424987591382	7.6259398381058014
6	14.097014475350477	1.9989145453544375	0.3746653836977297	83.52940559559735
7	0.6418936267395317	1.5132473331009002	96.72485601312437	1.1200030270352082
8	96.22926093514329	2.2597679699544835	0.6190366305143923	0.891934464387836
9	20.317185951493162	39.10355766817351	19.792424073839275	20.78683230649406
10	22.82973861027445	34.17654864460686	22.69335678328454	20.300355961834153
11	23.056760875696348	33.34808179444785	20.28612014562165	23.309037184234153
12	23.255019664665703	32.07643345063134	19.250155247360794	25.418391637342165
13	26.122719404603224	28.327174929228544	25.704244716036907	19.845860950131332
14	21.273676567938672	22.885925388114202	25.99964765423135	29.840750389715776
15	24.528111488593172	26.78153276115908	30.64539082330476	18.044964926942992
16	34.443610705211746	29.589142082329555	18.125403156800022	17.841844055658676
17	25.33630372515078	31.198687645705743	19.048801881593143	24.41620674755033
18	26.866838163363543	26.701207550685556	21.799317808914033	24.63263647703687
19	23.61014436126802	25.45845505756079	23.774197137615438	27.157203443555755
20	21.68981140081919	22.569544169053586	28.29168833655471	27.44895609357252
21	23.432199771786973	26.82563955278526	25.269806277669538	24.472354397758235
22	21.619600404718962	29.364896581884427	23.214151707505156	25.80135130589146
23	20.902968063113065	28.453403918950738	19.41466721520592	31.228960802730278
24	26.301939810753954	26.7221415800518	20.131131931453293	26.84478667774095
25	22.135370173436726	32.755297155616965	23.25884066745843	21.850492003487883
26	23.064523223651356	28.375780439400643	23.85343652081215	24.70625981613585
27	23.054947550185744	26.034540572367014	27.270999067969576	23.639512809477665
28	24.119167565805306	28.070202674905104	25.84965366990874	21.96097608938085
29	19.9727800331711	24.910150822420764	34.84440373524183	20.272665409166297
30	17.279543774421796	27.021649593410075	30.722990812123776	24.975815820044357
31	22.329747898289913	27.79561726494355	28.961034592505	20.913600244261534
32	23.252215537874378	28.181383013131512	30.746102593958213	17.8202988550359
33	19.454815964810145	31.28869913965974	29.579662332621776	19.676822562908338
34	16.73329348661383	38.4645040765264	26.29340122800345	18.508801208856323
35	16.494730659463208	37.726304786005	26.54464255681392	19.23432199771787
36	17.833735681702333	37.56044928469964	27.76669486986424	16.839120163733792
37	17.673572568897306	37.7485568501427	28.831171519427862	15.746699061532132
38	16.681285755315265	39.96522468116156	28.660917354281324	14.692572209241852
39	16.90639384601054	40.97639987476745	27.088783148460077	15.028423130761926
40	16.5423397269206	40.91507732592288	27.45102605302719	15.091556894129335
41	15.482520486129978	43.25490774449455	26.970536714612102	14.292035054763364
42	16.06055666384635	44.245642088485596	25.627391773463636	14.066409474204425
43	15.869085414289447	43.446120249119616	25.794023509564507	14.890770827026426
44	15.532717002905706	41.9733440971225	26.962774366657094	15.531164533314703
45	15.2804406943679	41.5120018836631	26.980110277089945	16.22744714487905
46	15.566353844044079	42.33791570607611	27.08076205557324	15.014968394306576
47	15.83234363396907	42.742075289600265	26.15549017933611	15.270090897094555
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	8.0
8	15.0
9	15.0
10	50.0
11	85.0
12	213.0
13	341.0
14	544.5
15	748.0
16	1086.0
17	1424.0
18	1994.5
19	2565.0
20	3352.5
21	4140.0
22	5258.5
23	6377.0
24	7887.0
25	9397.0
26	9397.0
27	10450.0
28	11503.0
29	12556.0
30	13609.0
31	14831.5
32	16054.0
33	17277.0
34	18500.0
35	20505.5
36	22511.0
37	25258.0
38	28005.0
39	30948.0
40	33891.0
41	36928.0
42	39965.0
43	39965.0
44	41561.5
45	43158.0
46	41356.0
47	39554.0
48	35427.5
49	31301.0
50	27760.0
51	24219.0
52	20508.5
53	16798.0
54	13372.0
55	9946.0
56	7737.0
57	5528.0
58	5528.0
59	4236.0
60	2944.0
61	2368.0
62	1792.0
63	1365.5
64	939.0
65	670.5
66	402.0
67	314.5
68	227.0
69	201.0
70	175.0
71	177.0
72	179.0
73	127.0
74	75.0
75	75.0
76	59.5
77	44.0
78	36.0
79	28.0
80	22.5
81	17.0
82	13.5
83	10.0
84	12.0
85	14.0
86	7.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	15.549276678543059
6	1.3128717841239284
7	4.264633966482181
8	22.631901697625498
9	0.005174898636672954
10	1.7245349706712618
11	0.0
12	2.587449318336477E-4
13	1.194884095207785
14	3.065868697296892
15	5.174898636672954E-4
16	1.7247937156030957
17	0.0
18	0.02044084961485817
19	0.005174898636672954
20	0.0
21	0.0
22	0.010349797273345908
23	0.0
24	0.0
25	0.0
26	0.0
27	2.002168282528766
28	0.0
29	0.0
30	38.74860601167975
31	0.002587449318336477
32	0.0015524695910018862
33	0.0015524695910018862
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
47	386481.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.495361147147996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.76858682981393	52.97643447862595
2	8.332549692556386	10.414914046257365
3	2.6482043595254936	4.965014635199924
4	1.139379996529151	2.8482385746770205
5	0.6467627849010811	2.0209836909464127
6	0.43461902433554495	1.6297003732362587
7	0.29071468952989704	1.2717823659066343
8	0.2353920398617948	1.1768728433861377
9	0.17725100912600336	0.9969629246123404
>10	1.2307575272757543	14.574382198262887
>50	0.0702091700402543	2.999940698332608
>100	0.022318146767758562	2.6776882540808633
>500	0.0023248069549748505	0.8243734755102993
>1k	9.299227819899402E-4	0.6227114409653054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTATAGTGGCACTCTTGGTCCGACGATCGTCGGACTGTAGAACTCTG	1136	0.2939342425630238	Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGAAACTCAGGGTCCGACGATCGTCGGACTGTAGAACTCTG	1007	0.2605561463564833	Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGACTGATAGAGTACCGTCAACAGTCTAGGTACGTACTCTATTGA	650	0.168184205691871	No Hit
CTATAGTGGCACTCTTGGTCCGACGATCGNCGGACTGTAGAACTCTG	586	0.15162453005451756	Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTATAGTGAAACTCAGGGTCCGACGATCGNCGGACTGTAGAACTCTG	569	0.14722586621334555	Illumina DpnII expression Sequencing Primer (95% over 23bp)
CTATAGTGTAACACTAACAGTCCGACGATCGTCGGACTGTAGAACTC	519	0.13428861962166316	Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTAGACTGACCGACTCTGTTGACGATCGTCGGACTGTAGAACTCTGA	513	0.13273615003066128	Illumina DpnII expression Sequencing Primer (100% over 24bp)
CTAGACTGAACAGTTACAACAGTCTAGGTACGTAACTGTTTGACGAT	438	0.11333028014313769	No Hit
CTATAGTGCAAACTAGAGTCCGACGATCGTCGGACTGTAGAACTCTG	418	0.10815538150646474	Illumina DpnII expression Sequencing Primer (100% over 23bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0012937246591682384	0.0	0.0
16	0.0	0.0	0.04812655732105847	0.0	0.0
17	0.0	0.0	0.10298048286979179	0.0	0.0
18	0.0	0.0	0.3198087357463886	0.0	0.0
19	0.0	0.0	0.4525448857770498	0.0	0.0
20	0.0	0.0	0.6486735441069548	0.0	0.0
21	0.0	0.0	0.8370398544818504	0.0	0.0
22	0.0	0.0	0.9407965721471431	0.0	0.0
23	0.0	0.0	1.0430008202214338	0.0	0.0
24	0.0	0.0	1.6696810451225286	0.0	0.0
25	0.0	0.0	2.765207086506193	0.0	0.0
26	0.0	0.0	3.232241688465927	0.0	0.0
27	0.0	0.0	3.83822231882033	0.0	0.0
28	0.0	0.0	4.279382427596699	0.0	0.0
29	0.0	0.0	4.553652055340366	0.0	0.0
30	0.0	0.0	4.7730677575352995	0.0	0.0
31	0.0	0.0	5.183954709287132	0.0	0.0
32	0.0	0.0	5.4590005718263	0.0	0.0
33	0.0	0.0	5.834180722985089	0.0	0.0
34	0.0	0.0	6.1477795803674695	0.0	0.0
35	0.0	0.0	6.77057863129106	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCCT	15	0.0040050442	60.334785	25
TCAACGT	15	0.0045657195	58.371647	28
CACGCAT	25	8.327164E-6	58.371643	29
AGTGTCG	30	2.0121763E-5	50.28997	27
ACTGCGA	345	0.0	50.268017	5
ACTGCCA	270	0.0	50.268013	5
GACCGGA	15	0.008248089	50.268013	4
CCTGTGG	25	2.018449E-5	50.268013	5
CCTGTAT	15	0.008248089	50.268013	5
GCCTGGA	75	0.0	50.268013	4
ACTACAA	15	0.008248089	50.268013	5
CCTGCGG	40	2.5465852E-9	50.268013	5
CCTGCGC	15	0.008248089	50.268013	5
GACTATT	20	4.0695316E-4	50.268013	4
GACTAGG	30	1.0071253E-6	50.268013	4
ACTGCGT	170	0.0	50.26801	5
ACTGAGC	385	0.0	50.26801	5
AGTGGGG	85	0.0	50.26801	5
GACTGCT	1080	0.0	50.035294	4
GACTGAG	1560	0.0	49.784664	4
>>END_MODULE