FastQCFastQC Report
Thu 26 May 2016
SRR2099066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766178
Sequences flagged as poor quality0
Sequence length42
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAGTCCGAGGTATCGCGCTCGAACAACCGGCGCTGATT27170.35461733435311377No Hit
CTACGAGTGATATACGAGTTTTTTTTTTTTTTTTTTTTTTTT9080.11851032005617493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA4750.044.04839320
GCGCTCG4850.043.1401819
GCTCGAA4950.042.26865821
CTCGAAC4950.041.75738522
TCGAACA5050.040.43041223
ACACCGG453.568457E-739.21029324
CGAACAA5300.038.52331524
CTACGCA650.035.2119641
CGACGCG405.9226295E-835.211961
GAACAAC5900.035.03292525
CGTCCGC301.2134677E-535.010356
CGTCGCG358.673942E-735.0103456
CTACGCG25550.035.0052381
CGCGTTG1850.034.8660354
CGCGTAC1100.034.8660354
ACGAGGG1700.034.8660353
GCGTACT454.6766218E-934.8660355
ACGCGTT6200.034.8660353
CGAGCGT200.002464781334.866034
ACGAGTA81900.034.8021773
Produced by FastQC (version 0.11.5)