##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	766178
Sequences flagged as poor quality	0
Sequence length	42
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.58836849922603	30.0	27.0	32.0	20.0	35.0
2	28.14723341051296	30.0	27.0	31.0	17.0	36.0
3	27.11843461963147	29.0	25.0	31.0	16.0	35.0
4	28.84346065796721	30.0	27.0	31.0	21.0	35.0
5	27.47421486912963	30.0	25.0	31.0	14.0	37.0
6	21.57379355711075	24.0	15.0	29.0	7.0	31.0
7	26.131384612975054	28.0	23.0	31.0	15.0	35.0
8	26.161931039523452	28.0	23.0	31.0	12.0	37.0
9	25.91052340317785	27.0	26.0	27.0	20.0	31.0
10	26.04629603042635	27.0	26.0	27.0	20.0	31.0
11	25.485564712116506	27.0	26.0	27.0	16.0	31.0
12	27.964324217087935	30.0	26.0	31.0	18.0	35.0
13	30.851885593165036	31.0	31.0	31.0	28.0	34.0
14	30.76334350503408	31.0	31.0	34.0	28.0	34.0
15	30.987318873682096	31.0	31.0	34.0	28.0	34.0
16	34.928326054781	35.0	35.0	37.0	32.0	37.0
17	35.01011514295634	35.0	35.0	37.0	32.0	37.0
18	34.706459856586854	35.0	35.0	37.0	32.0	37.0
19	34.67159328511129	35.0	35.0	37.0	32.0	37.0
20	34.69233389630086	35.0	35.0	37.0	32.0	37.0
21	36.24910529929077	37.0	35.0	39.0	32.0	39.0
22	36.127872374304665	37.0	35.0	39.0	32.0	39.0
23	36.40379781199669	37.0	35.0	39.0	32.0	39.0
24	36.58605441555357	37.0	35.0	39.0	32.0	39.0
25	22.99920253518112	32.0	2.0	37.0	2.0	39.0
26	29.665331032736518	34.0	17.0	38.0	17.0	40.0
27	34.142930232922375	36.0	32.0	39.0	27.0	40.0
28	36.1792768782189	37.0	34.0	39.0	32.0	40.0
29	36.897112681387355	38.0	36.0	39.0	32.0	40.0
30	37.202635679959485	38.0	36.0	40.0	33.0	40.0
31	37.49817666390839	39.0	36.0	40.0	33.0	41.0
32	37.55823189911483	39.0	37.0	40.0	33.0	41.0
33	37.51523797342132	39.0	36.0	40.0	33.0	41.0
34	37.48790359420396	39.0	36.0	40.0	33.0	41.0
35	37.3764190566683	39.0	36.0	40.0	32.0	41.0
36	37.326411095071904	39.0	36.0	40.0	32.0	41.0
37	37.21464333353346	39.0	36.0	40.0	32.0	41.0
38	37.06183289000728	39.0	36.0	40.0	32.0	41.0
39	36.975133715663986	39.0	36.0	40.0	32.0	41.0
40	36.938472522051015	39.0	36.0	40.0	32.0	41.0
41	36.88734993695982	39.0	36.0	40.0	31.0	41.0
42	36.902170252865524	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.8054332254643555
1101	2	6.659925253704419
1101	3	6.0250416097618995
1101	4	5.422786791159783
1101	5	8.79721108802115
1101	6	7.475555831287803
1101	7	6.669042459218918
1101	8	7.068805368555964
1101	9	6.208773914861631
1101	10	5.745490018814316
1101	11	3.81070972657745
1101	12	8.208981640702468
1101	13	0.2780764265564102
1101	14	1.1515805476944792
1101	15	1.6167263430118837
1101	16	0.9471101452250039
1101	17	0.4122616259551961
1101	18	0.23548486390777867
1101	19	0.08136844266196874
1101	20	0.11678259894152632
1101	21	0.07791406671915269
1101	22	0.970279129432825
1101	23	0.497563724585369
1101	24	0.24486482088282457
1101	25	12.12889640263981
1101	26	7.217789575300326
1101	27	3.1349674563458265
1101	28	1.4502192496645634
1101	29	0.8129405194586568
1101	30	0.4875187948670501
1101	31	0.22823456330460345
1101	32	0.13561240926402007
1101	33	0.0548265748111163
1101	34	0.18686552546172663
1101	35	0.0033719911434246796
1101	36	-0.0939116000158009
1101	37	-0.045494732791311776
1101	38	-0.18548412981211726
1101	39	-0.2953958154996812
1101	40	-0.5090013026837497
1101	41	-0.4795530139036188
1101	42	-0.43802793251793304
1102	1	4.333442686352591
1102	2	3.701000614856156
1102	3	2.8457377136508875
1102	4	1.9144290797415273
1102	5	4.75495106150656
1102	6	3.886193372990764
1102	7	3.1386016952261713
1102	8	3.5196431255530243
1102	9	4.45115952960024
1102	10	3.6813564816911466
1102	11	4.022974026104681
1102	12	5.951665133725953
1102	13	-0.11462233700903468
1102	14	1.282909605276064
1102	15	1.752953615241136
1102	16	1.024050736653777
1102	17	0.3823087660497535
1102	18	0.12414960224053573
1102	19	0.11719772211151991
1102	20	0.10162915396204397
1102	21	0.18010104115229097
1102	22	0.9631091648769114
1102	23	0.6909548668493031
1102	24	0.3864182938012348
1102	25	12.143628709354466
1102	26	7.194054154216847
1102	27	3.224548701107281
1102	28	1.4687226269880185
1102	29	0.8816223498419689
1102	30	0.5359038152186457
1102	31	0.2913097765443666
1102	32	0.2842834612468579
1102	33	0.31199996325535295
1102	34	0.15125400531009348
1102	35	0.10143557685841387
1102	36	-0.0050106818659116925
1102	37	-0.09391193769322115
1102	38	-0.1983908192681838
1102	39	-0.12646321953494066
1102	40	-0.17057356590132855
1102	41	-0.09769051451991828
1102	42	-0.06423793465444305
1103	1	1.7145306837774505
1103	2	0.5057593964921772
1103	3	-0.29074963194119263
1103	4	0.24648195062230727
1103	5	1.8959115089374556
1103	6	0.6015890998647819
1103	7	-2.4167803512068566
1103	8	-0.9720858407689512
1103	9	2.547806212093299
1103	10	1.6043810662483438
1103	11	-0.9758100241105865
1103	12	2.1545875173416142
1103	13	-0.8135950968368242
1103	14	1.1989672240746003
1103	15	1.6015980444850904
1103	16	0.9223137230135734
1103	17	0.27696212781694385
1103	18	0.14331066931493552
1103	19	0.07974831962489759
1103	20	0.1422407501476144
1103	21	-0.044824722337025946
1103	22	0.8161747907570884
1103	23	0.5406675775884437
1103	24	0.29437145840913104
1103	25	12.231599980428378
1103	26	7.155055642970719
1103	27	3.2147100498378194
1103	28	1.369284329800557
1103	29	0.6339717643995613
1103	30	0.303469903819618
1103	31	0.14863645742796905
1103	32	0.056106581192139515
1103	33	0.07089707829176461
1103	34	0.10098200595387397
1103	35	-0.061288037996931166
1103	36	-0.0897425049858569
1103	37	-0.02282434263427291
1103	38	0.004033435348965497
1103	39	-0.05517645334977317
1103	40	0.05544381630605244
1103	41	0.10236903563499311
1103	42	-0.10421540216339054
1104	1	1.3341336095872727
1104	2	1.0853936702643807
1104	3	-0.387949213968362
1104	4	-0.6336643398688118
1104	5	1.4044486040262747
1104	6	0.37004260038724723
1104	7	-0.5151711558045555
1104	8	-0.17608793063310912
1104	9	2.3021531295436617
1104	10	1.0681532710550314
1104	11	0.7264470291809495
1104	12	2.7591747691702437
1104	13	0.315871987802673
1104	14	1.9250173808144098
1104	15	1.83577777280275
1104	16	0.9641214555109627
1104	17	0.3134929533132862
1104	18	0.06019677171828164
1104	19	0.012506940314551684
1104	20	0.003724553700379829
1104	21	-0.005420333622289775
1104	22	0.8328623560653483
1104	23	0.4357145995320195
1104	24	0.25137250334120864
1104	25	12.145528925046982
1104	26	7.095295977348979
1104	27	3.165859475125174
1104	28	1.3868496380555158
1104	29	0.674400186552127
1104	30	0.28010522252390047
1104	31	0.24084126411553086
1104	32	-0.021866250573793877
1104	33	-0.020806160997679513
1104	34	-0.07088842246827909
1104	35	-0.21065062942849266
1104	36	-0.04993059276014833
1104	37	-0.05844607722152517
1104	38	-0.1889028237941872
1104	39	-0.35368220047620724
1104	40	-0.38127509696458617
1104	41	-0.3152903165071166
1104	42	-0.3786041168969376
1105	1	0.739075334528998
1105	2	1.1487036935744044
1105	3	-2.6476461836653336
1105	4	-0.7690411852456549
1105	5	-1.5366702770926075
1105	6	-0.5139900336453849
1105	7	-1.4009831216165232
1105	8	-4.9051244496696285
1105	9	1.7602494776400128
1105	10	0.699582952484711
1105	11	1.0665713493052706
1105	12	3.1772400372355136
1105	13	0.1305339924646809
1105	14	1.7228340086310396
1105	15	1.5841149911399661
1105	16	0.8142146956041998
1105	17	0.22201665183698793
1105	18	-0.17507129354978446
1105	19	-0.07723664942903952
1105	20	-0.061458041482211456
1105	21	-0.17151201971397256
1105	22	0.821176264379254
1105	23	0.47625850007592163
1105	24	0.2704634124321217
1105	25	12.044526594044648
1105	26	6.8173161793691825
1105	27	2.9758594751251692
1105	28	1.3058006870065668
1105	29	0.47047633262827304
1105	30	0.1579762403949161
1105	31	0.2649671382414027
1105	32	-0.1408872295947745
1105	33	-0.2996717398632569
1105	34	-0.25370116528101505
1105	35	-0.2415208702987357
1105	36	-0.18954209237164577
1105	37	-0.2921523709278233
1105	38	-0.30777617266753765
1105	39	-0.4965881833821868
1105	40	-0.49394020962969876
1105	41	-0.3073804485972502
1105	42	-0.2534137517065673
1106	1	-2.8372991852601572
1106	2	-4.003503489486437
1106	3	-1.8748935704249128
1106	4	-6.010596026068789
1106	5	-0.510268954837624
1106	6	0.35168762203226933
1106	7	-1.407041074503745
1106	8	-4.308212838245822
1106	9	-0.2668021022164453
1106	10	0.2681585503286037
1106	11	0.7135423728616601
1106	12	3.0553678849975086
1106	13	0.19564075562022154
1106	14	1.8404750938330992
1106	15	1.729930977321807
1106	16	0.8072884917267729
1106	17	0.1928884764893013
1106	18	-0.04389466529998742
1106	19	0.03740241069782968
1106	20	0.0043253350328740225
1106	21	-0.14774374192131035
1106	22	0.7519743822505447
1106	23	0.45943965496439176
1106	24	0.1645342602834532
1106	25	12.147393564472594
1106	26	6.97366732145203
1106	27	3.02342625793586
1106	28	1.260286973078216
1106	29	0.4981595572627171
1106	30	0.24395170124842736
1106	31	-0.0740885215459599
1106	32	-0.09506126693954542
1106	33	-0.14744907093326987
1106	34	-0.06433578811076046
1106	35	-0.12232732671494517
1106	36	-0.2829492813885963
1106	37	-0.24098065646342803
1106	38	-0.27817850746011885
1106	39	-0.12109799606273697
1106	40	-0.15030951782827628
1106	41	-0.08868647319595624
1106	42	-0.21833170638062427
1107	1	2.5625125664420665
1107	2	1.8745296396234004
1107	3	0.48628194520703616
1107	4	-4.443430026474648
1107	5	-23.396157456579786
1107	6	-18.041169520824873
1107	7	-8.498814278481138
1107	8	-5.203575820686055
1107	9	1.203172618109619
1107	10	1.4888753618514698
1107	11	2.094235696523519
1107	12	4.015589098335504
1107	13	0.27483672814287985
1107	14	1.6659039415894483
1107	15	1.5036892293759152
1107	16	0.7616172311108258
1107	17	0.28373177273426364
1107	18	0.06228644165181407
1107	19	-0.05785241532361596
1107	20	0.1473012124815014
1107	21	0.0018073333823309667
1107	22	0.8495256432119049
1107	23	0.651992621386249
1107	24	0.3064485425436416
1107	25	12.313679201041118
1107	26	7.147245960451382
1107	27	3.066268397058259
1107	28	1.5366919271171398
1107	29	0.7215613896612822
1107	30	0.3045031046912996
1107	31	-0.05212370152601409
1107	32	-0.16451128831177186
1107	33	-0.4561131340741653
1107	34	-0.4445822379464772
1107	35	-0.5503633702193014
1107	36	-0.264534625654143
1107	37	-0.3841664782579173
1107	38	-0.5181367992363448
1107	39	-0.07417786342540467
1107	40	-0.2717561153266459
1107	41	-0.2525461461793057
1107	42	-0.04185972126778381
1113	1	-11.198507083053421
1113	2	-9.444995940125228
1113	3	-0.2552652312843797
1113	4	0.40297346676899437
1113	5	2.8895568291344986
1113	6	-18.041169520824873
1113	7	-8.86755210818551
1113	8	0.336047711502534
1113	9	-20.996331718941185
1113	10	-21.696998244096484
1113	11	-11.615919493185572
1113	12	-22.19506765507218
1113	13	0.280619462550149
1113	14	2.119317525114557
1113	15	0.928779215804191
1113	16	1.0410767224662294
1113	17	-0.4653093554890191
1113	18	-0.8965853350638255
1113	19	0.21853868634629947
1113	20	0.5805932405690726
1113	21	0.7181583099563511
1113	22	1.6889662521692443
1113	23	1.298773762591182
1113	24	0.7047491267178358
1113	25	12.76750583702389
1113	26	7.948744750797754
1113	27	4.065383284648981
1113	28	1.3455076467135285
1113	29	0.9627840249359636
1113	30	1.2140446164632976
1113	31	0.33773881101307524
1113	32	0.12338627467872954
1113	33	0.7454564652649509
1113	34	0.6989239873441377
1113	35	0.525756296978436
1113	36	0.873070850241298
1113	37	0.7311931724177185
1113	38	1.0975473926560255
1113	39	1.424730497936494
1113	40	0.66894135325186
1113	41	0.978822237605435
1113	42	1.4129110346182188
1114	1	-9.48903137357771
1114	2	-0.5989160940453822
1114	3	-1.3995653755845225
1114	4	-8.487839424043894
1114	5	-0.7597938202161494
1114	6	-1.2027856824410357
1114	7	-0.4808277214611216
1114	8	2.5053598808147015
1114	9	-5.370069092678559
1114	10	0.7407121936139518
1114	11	1.6067837295176481
1114	12	-22.19506765507218
1114	13	-0.3187071367764496
1114	14	-27.388618982821953
1114	15	-13.857656570631594
1114	16	-6.677056995667492
1114	17	-2.941018831198498
1114	18	-1.4093318478103427
1114	19	-1.093149625342015
1114	20	-0.5237838638080348
1114	21	-0.571884980086935
1114	22	0.39555595875894767
1114	23	0.1424486062660293
1114	24	-0.21829079632208703
1114	25	11.983475052993107
1114	26	6.606272408325413
1114	27	2.457879677145378
1114	28	0.6129439141497954
1114	29	0.2523463144982543
1114	30	-0.13612373370505537
1114	31	-0.47515200187773843
1114	32	-0.46247231117985876
1114	33	-0.7225570027485233
1114	34	0.019751308171457538
1114	35	-0.29627352505139015
1114	36	-0.5261595489891064
1114	37	-0.5723181310935814
1114	38	-0.10543481032618018
1114	39	-0.3881603149543196
1114	40	-0.17184748753697932
1114	41	-0.013000754217550536
1114	42	-0.18978256807538685
1115	1	-9.313745178291516
1115	2	-2.4597578448871324
1115	3	-1.2976461836653321
1115	4	-0.25972494592941686
1115	5	-2.7461574565797875
1115	6	3.988830479175128
1115	7	2.9414955108621115
1115	8	3.52239691785174
1115	9	-20.996331718941185
1115	10	-21.696998244096484
1115	11	-8.647189334455414
1115	12	-22.19506765507218
1115	13	0.37950835143903916
1115	14	1.8413810171780476
1115	15	-27.48391919689422
1115	16	-15.16527248388298
1115	17	-4.411658561838227
1115	18	-1.2973789858574776
1115	19	-1.0274930596854475
1115	20	-0.36051787054204
1115	21	-0.5672385154404722
1115	22	0.5013472045501928
1115	23	0.314805508622932
1115	24	0.41713007909878286
1115	25	12.204013773531827
1115	26	7.18953840159141
1115	27	3.049192808458507
1115	28	1.561698122904005
1115	29	0.7250062471581842
1115	30	0.2807112831299605
1115	31	0.24932611260037874
1115	32	0.21783071912317098
1115	33	0.2543453541538341
1115	34	-0.2169490285288802
1115	35	0.7814705826927195
1115	36	0.18370577087621598
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2206	39	0.17777674301007096
2206	40	0.0789641322951411
2206	41	0.08328042179027051
2206	42	0.08641141426893739
2207	1	0.3385780540317178
2207	2	0.3276832325539445
2207	3	-3.908656284675434
2207	4	-1.238007774212246
2207	5	-0.984709645131975
2207	6	-0.3569944366497886
2207	7	-2.546820987454389
2207	8	-1.0326872572324355
2207	9	1.2400319174224528
2207	10	1.0875135404153013
2207	11	1.453248375982298
2207	12	1.4958414358369119
2207	13	0.22405380598449298
2207	14	1.8127278185248485
2207	15	1.750424237449213
2207	16	0.9512258326153358
2207	17	0.24214615196648737
2207	18	0.038479600001110725
2207	19	0.05499852280613737
2207	20	-0.04970978973396001
2207	21	-0.1260937342956936
2207	22	0.903300066503057
2207	23	0.40844187225929574
2207	24	0.08069910266780767
2207	25	6.3255625950806476
2207	26	3.9732757753287835
2207	27	1.7737045929702902
2207	28	0.6701829713888543
2207	29	0.3331543953063303
2207	30	0.1541119565306346
2207	31	0.21979749307175922
2207	32	-0.057758506466051074
2207	33	-0.25589033608185474
2207	34	-0.09068640226625746
2207	35	0.043793815015952475
2207	36	-0.2642066870362427
2207	37	-0.29285685163230113
2207	38	-0.18489608978745764
2207	39	-0.0615609883549979
2207	40	-0.07151078720028181
2207	41	-0.24599738721418873
2207	42	-0.1931495714423903
2208	1	0.3938505338064928
2208	2	-24.349757844887133
2208	3	-9.32096930770821
2208	4	-3.0005365845205354
2208	5	2.9376863412762617
2208	6	4.240606895714176
2208	7	3.4635935200504733
2208	8	-23.45760308214826
2208	9	3.3604829824370697
2208	10	2.9170905767304696
2208	11	-21.457189334455414
2208	12	-7.391086031794998
2208	13	0.5860474019750264
2208	14	2.2346581994138823
2208	15	2.1454835596142026
2208	16	1.4181884655810322
2208	17	0.9148651747620775
2208	18	0.6716102943875484
2208	19	0.7159985176499291
2208	20	0.6762202611578019
2208	21	0.7579988505625934
2208	22	1.5081159641214015
2208	23	1.197944865437627
2208	24	1.018538961181477
2208	25	-22.295986226468173
2208	26	-13.232008305912423
2208	27	-4.888601984803362
2208	28	-1.579526991950516
2208	29	-0.4489906900546785
2208	30	0.24365155878080458
2208	31	0.556630859920439
2208	32	0.7883513929363417
2208	33	0.9084648028521514
2208	34	1.0827446927574869
2208	35	0.9998319915748013
2208	36	1.008100870416797
2208	37	1.0819066073578014
2208	38	1.1324777507104287
2208	39	1.1898195861496248
2208	40	1.1939905766165495
2208	41	1.2993210343676367
2208	42	1.238842632372176
2209	1	-2.5378923354821517
2209	2	-3.978520386693152
2209	3	-5.140756551558308
2209	4	-5.775611233554834
2209	5	-3.446324680660055
2209	6	0.6110043922186037
2209	7	-7.154859004188058
2209	8	-5.293723483486055
2209	9	-3.5180708493759667
2209	10	-0.8274330267051795
2209	11	-2.744814752515616
2209	12	-1.9877097955404075
2209	13	-0.15156188267467385
2209	14	1.3271000807231985
2209	15	1.3856460204970844
2209	16	0.16917567665213795
2209	17	-0.12650806016598892
2209	18	-0.20958634371700668
2209	19	-1.002409447645313
2209	20	-0.8047653621808379
2209	21	-0.7727903549053536
2209	22	-0.35865279544980666
2209	23	-0.6876359629489741
2209	24	-1.2124351382925198
2209	25	-4.342808968943089
2209	26	-2.7750435382079246
2209	27	-1.4509409708057106
2209	28	-1.395693181443825
2209	29	-1.0022847227414786
2209	30	-1.4637703222212082
2209	31	-1.5078310780350748
2209	32	-1.0107645986025773
2209	33	-1.1449188598929894
2209	34	-2.3336714365556404
2209	35	-0.9276598520898887
2209	36	-1.166160449859568
2209	37	-1.02396953926673
2209	38	-1.0277910370302266
2209	39	-1.0908505393836734
2209	40	-2.3473032716884177
2209	41	-2.456915937263169
2209	42	-2.0843018973773226
2215	1	-12.161962232555082
2215	2	-3.907897379770855
2215	3	-1.7657857185490542
2215	4	-0.23453114747980308
2215	5	-0.016312495339477096
2215	6	4.710768463671251
2215	7	1.63521644109467
2215	8	2.666427925603678
2215	9	-20.996331718941185
2215	10	-10.627230802236019
2215	11	-2.8757939856182055
2215	12	-22.19506765507218
2215	13	0.09198897159407693
2215	14	-27.163812781271567
2215	15	-13.507175010847709
2215	16	-4.64589263892174
2215	17	-2.1600306548614867
2215	18	-0.9582316990357711
2215	19	0.04615035116726318
2215	20	0.06638135426415914
2215	21	0.2958622597533278
2215	22	1.0366960417594981
2215	23	0.2463558962198249
2215	24	-0.2731024790407517
2215	25	9.959827727020198
2215	26	6.041398866707684
2215	27	2.589192808458506
2215	28	1.0542562624388836
2215	29	0.6578744642124477
2215	30	-0.010761585087095682
2215	31	-0.2598986936011727
2215	32	0.025195060208446307
2215	33	0.262252330898022
2215	34	0.05514399472693299
2215	35	0.338602365638458
2215	36	0.0882794142870651
2215	37	0.01432347880383844
2215	38	0.0023092974179306225
2215	39	0.45499997006643156
2215	40	0.8727065802729044
2215	41	0.9976483727106427
2215	42	0.49818235244767095
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	49.0
25	485.0
26	3447.0
27	12262.0
28	27888.0
29	50455.0
30	76933.0
31	101434.0
32	124222.0
33	133232.0
34	109876.0
35	75256.0
36	32991.0
37	17025.0
38	622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.5486067424046183	1.417046854267	0.52627127156232	97.50807513176606
2	0.6227933684551408	1.6143130579931073	97.22619339109738	0.5367001824543667
3	2.453215832352273	93.77390110392102	0.6939640657915002	3.0789189979352054
4	0.6970964971586238	1.7987987125707081	0.8516297779367197	96.65247501233395
5	95.33867336900161	2.873874605454611	1.0432904985615983	0.7441615269821787
6	2.0196523587037203	90.63222188899852	1.99454749500956	5.353578257288214
7	95.94222079518846	2.669684320988621	0.5476677134481399	0.8404271703747791
8	1.3224720195672148	2.3844252130620114	95.2577411872497	1.0353615801210576
9	31.08930668568073	16.96507214159886	23.969387006123647	27.976234166596765
10	24.638090752080387	17.139922541476945	28.36342701627676	29.858559690165908
11	28.323697899519324	17.636136169091987	27.346678079936787	26.6934878514519
12	27.40871547017668	20.77401544030665	27.880056687268656	23.937212402248015
13	30.262012097284835	28.428358249875647	20.970628899533526	20.339000753305996
14	27.697145812169083	28.26572585831699	23.083536116383033	20.953592213130893
15	19.81552125439274	26.87278729401986	33.29255877352215	20.019132678065247
16	23.412444627749686	30.09052726650987	27.435269610978132	19.061758494762312
17	19.488276094355214	30.887507686041367	28.73606884604288	20.88814737356054
18	21.86854751767866	30.3161928429164	24.6004192237313	23.214840415673642
19	23.32421447757571	28.934268538120385	26.170550446501988	21.57096653780192
20	20.775981560420686	28.953585198217645	27.62386808287369	22.646565158487974
21	22.231518002344103	28.12114156240456	27.62151875934835	22.025821675902986
22	21.332126344376054	28.285119562957718	28.373801673118273	22.00895241954796
23	20.404788443416543	29.092586840133755	29.803518242497173	20.69910647395253
24	20.099506903095627	27.813510698558296	30.315540253048244	21.77144214529783
25	18.788089468038617	29.84704620396488	30.006157880100115	21.358706447896388
26	19.47787180946315	30.59600194212266	29.835076196989707	20.09105005142448
27	19.82708888581445	31.046994546930595	29.680333710097184	19.445582857157774
28	19.434894630089357	30.712072444587147	30.327164892881104	19.525868032442396
29	20.972444829620503	31.479093467263226	29.210542802399463	18.337918900716808
30	20.732127521280955	31.907729013362427	28.450699445820682	18.909444019535933
31	19.92957250142917	31.770685141050777	28.684587654565913	19.615154702954143
32	19.854655184565466	31.913210768254896	29.00135477656628	19.230779270613358
33	20.519513742237443	32.44950912190118	29.111381428336497	17.919595707524884
34	21.259681170694016	32.36897953217138	28.79905191743955	17.57228737969506
35	20.389126286580925	32.0114908023984	29.232632625838907	18.36675028518177
36	20.668304232175814	32.92642179754574	28.911688928682366	17.493585041596077
37	20.04938800122165	32.114469483592586	29.112164536178277	18.72397797900749
38	20.05852425937576	31.65165274910008	30.659063559642796	17.630759431881366
39	20.36654667714291	32.37942097006179	29.890312694961224	17.363719657834082
40	19.312091968184937	32.38137873966624	29.65746863000504	18.649060662143786
41	19.783783924884297	32.09436971565354	30.357567040557154	17.764279318905007
42	18.989321019397583	32.60573913633647	30.229659426399607	18.175280417866343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	3.5
7	7.0
8	36.5
9	66.0
10	66.0
11	220.5
12	375.0
13	830.0
14	1285.0
15	2422.5
16	3560.0
17	3560.0
18	6275.0
19	8990.0
20	9699.0
21	10408.0
22	10408.0
23	12581.5
24	14755.0
25	16341.0
26	17927.0
27	21621.5
28	25316.0
29	25316.0
30	29211.0
31	33106.0
32	38271.0
33	43436.0
34	43436.0
35	48145.0
36	52854.0
37	55902.0
38	58950.0
39	59940.0
40	60930.0
41	60930.0
42	60762.5
43	60595.0
44	60167.0
45	59739.0
46	59654.5
47	59570.0
48	59570.0
49	59382.5
50	59195.0
51	56170.0
52	53145.0
53	53145.0
54	47927.0
55	42709.0
56	39424.5
57	36140.0
58	31770.0
59	27400.0
60	27400.0
61	21093.5
62	14787.0
63	11647.5
64	8508.0
65	6621.5
66	4735.0
67	4735.0
68	3817.0
69	2899.0
70	2226.5
71	1554.0
72	1554.0
73	1304.0
74	1054.0
75	980.0
76	906.0
77	828.5
78	751.0
79	751.0
80	561.5
81	372.0
82	241.0
83	110.0
84	110.0
85	72.5
86	35.0
87	21.5
88	8.0
89	4.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	1.244358360589837
2	0.8530654756466514
3	0.0
4	0.0
5	1.9573780505313387
6	3.8201305701808193
7	0.0026103594726029724
8	0.8535875475411718
9	0.42209512671990057
10	0.24772311395002208
11	0.8911767239466547
12	0.8116912780058941
13	0.028844472172262846
14	0.558616927137036
15	0.12986538376199785
16	0.0
17	0.0233627172797966
18	0.0
19	0.0
20	0.0
21	0.0
22	0.8039907175617154
23	0.0
24	0.0
25	34.294641715110586
26	7.831078417808916E-4
27	5.220718945205945E-4
28	0.0026103594726029724
29	7.831078417808916E-4
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
42	766178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.56597200680055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.81054814499468	44.2167386434177
2	10.344067808209372	11.90932635767655
3	5.183928606080572	8.952536670686605
4	2.8834866808238457	6.639628542011508
5	1.6593430124169644	4.776084670123752
6	0.9886404569859645	3.4147229322986723
7	0.5742477626496338	2.3139991440759666
8	0.34087004339913524	1.5698012301017206
9	0.2359599815975963	1.2224939125835161
>10	0.8894485260766835	8.813686357364855
>50	0.0584610739548922	2.2731099129193004
>100	0.02991025709809487	3.1155424005594345
>500	8.157342844845622E-4	0.3570414095473747
>1k	2.719114281615208E-4	0.42528781663315085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTACGAGTCCGAGGTATCGCGCTCGAACAACCGGCGCTGATT	2717	0.35461733435311377	No Hit
CTACGAGTGATATACGAGTTTTTTTTTTTTTTTTTTTTTTTT	908	0.11851032005617493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	1.3051797363014862E-4	0.0	0.0
11	0.0	0.0	6.52589868150743E-4	0.0	0.0
12	0.0	0.0	0.031715867592126114	0.0	0.0
13	0.0	0.0	0.041896269535277704	0.0	0.0
14	0.0	0.0	0.04790009632226454	0.0	0.0
15	0.0	0.0	0.06930504399760891	0.0	0.0
16	0.0	0.0	0.08235684136062377	0.0	0.0
17	0.0	0.0	0.09527812075000848	0.0	0.0
18	0.0	0.0	0.10859095406028364	0.0	0.0
19	0.0	0.0	0.1254277726585728	0.0	0.0
20	0.0	0.0	0.13756594420617663	0.0	0.0
21	0.0	0.0	0.15153136738460254	0.0	0.0
22	0.0	0.0	0.2323219930616645	0.0	0.0
23	0.0	0.0	0.2631242348383796	0.0	0.0
24	0.0	0.0	0.2957537282459167	0.0	0.0
25	0.0	0.0	0.3394772494120165	0.0	0.0
26	0.0	0.0	0.3735424405294853	0.0	0.0
27	2.6103594726029723E-4	0.0	0.42353082442983225	0.0	0.0
28	2.6103594726029723E-4	0.0	0.4608589648880547	0.0	0.0
29	2.6103594726029723E-4	0.0	0.5177648013907995	0.0	0.0
30	2.6103594726029723E-4	0.0	0.5801523927860106	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTCGA	475	0.0	44.048393	20
GCGCTCG	485	0.0	43.14018	19
GCTCGAA	495	0.0	42.268658	21
CTCGAAC	495	0.0	41.757385	22
TCGAACA	505	0.0	40.430412	23
ACACCGG	45	3.568457E-7	39.210293	24
CGAACAA	530	0.0	38.523315	24
CTACGCA	65	0.0	35.211964	1
CGACGCG	40	5.9226295E-8	35.21196	1
GAACAAC	590	0.0	35.032925	25
CGTCCGC	30	1.2134677E-5	35.01035	6
CGTCGCG	35	8.673942E-7	35.010345	6
CTACGCG	2555	0.0	35.005238	1
CGCGTTG	185	0.0	34.866035	4
CGCGTAC	110	0.0	34.866035	4
ACGAGGG	170	0.0	34.866035	3
GCGTACT	45	4.6766218E-9	34.866035	5
ACGCGTT	620	0.0	34.866035	3
CGAGCGT	20	0.0024647813	34.86603	4
ACGAGTA	8190	0.0	34.802177	3
>>END_MODULE