FastQCFastQC Report
Thu 26 May 2016
SRR2099065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657191
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCAAAAACGACTCACTATAGTGAGTCGTATTACCGGC19160.11561733077237325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC800.034.942386
TGCTCCG45800.034.6995123
CTCACGT26900.034.6227045
TGCTCAC299150.034.574963
GCTCACG84850.034.5468374
CTCACCG23150.034.5160565
TGCTCAA250000.034.3261383
TGCTCAG363750.034.3206373
GCTCACC78300.034.2132954
GCTCCGG11550.034.2106974
GCTCCGC13000.034.144044
GCTCAAT61700.034.136444
CTCCGGA2500.034.1205145
GGTGCTC40900.034.0198361
ATGCTCC142300.034.018952
GATGCTC1254250.034.0109251
GCTCAAC58050.034.0039144
GCTCAGG104800.033.9498374
GATACTC20600.033.943121
CTCAACG17100.033.9006165
Produced by FastQC (version 0.11.5)