FastQCFastQC Report
Thu 26 May 2016
SRR2099064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283951
Sequences flagged as poor quality0
Sequence length42
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACAGAGTGGGAAAAGGAAAAGGAAAAGGAAAAGGAAAG6070.21376927709358304No Hit
TGCAACAGAAGCGGATCAGGAAGGCGTAATTCATCGATTTTG4820.16974759729671668No Hit
TTCGACAGAGTGGGAAAAGGAAAANGAAAAGGAAAAGGAAAG4700.1655215160362175No Hit
TGCTACGGCAGAGGGCCGGACGGCTGACGCGAGAGTGGTTGG4340.15284327225472No Hit
TGCTACAGGTGGAGACAAACCAGTAGATCCAGACAAACCAGT4160.14650415036397124No Hit
TGCTACAGTTTGAGTGAAGGATGTGTAGAGGTAGTGCAAGCT4090.14403893629534673No Hit
TGCTACATAGTCATGACTGAGCATGTGGTCAATCTTAGACTA3980.14016502847322249No Hit
TGCAACAGAAGCGATCAGGAAGGCGTAATTCATCGATTTTGG3910.13769981440459797No Hit
TTCGACAGTGAGGGTCGTGGTATGGTAAGAATTTTATTTTGG3850.13558677377434838No Hit
TGCTACAGTCGTCAGTCCGACGATCCCGATCGTCGGACTGTA3790.1334737331440988No Hit
TGCTACAGAAGATACAGTCCGACGATCGGACTGCAGAACTCT3410.12009114248585143No Hit
TGCAACAGAAGCGGATCAGGAAGGNGTAATTCATCGATTTTG3340.11762592841722691No Hit
TTCGACAGTGAGGTCGTGGTATGGTAAGAATTTTATTTTGGA3280.11551288778697733No Hit
TGCTACAGGCCGACGATCTATTTGCTACAGTTTTTTTTTTTT3030.10670855182760405No Hit
TTCGACAGACCGTACCATTGGCTTCTAAGTTTTTCTTTTGGC2990.10529985807410434No Hit
TTCGACAGTGAGGGTCGTGGTATGNTAAGAATTTTATTTTGG2990.10529985807410434No Hit
TGCTACAGTTTGAGTGAAGGATGTNTAGAGGTAGTGCAAGCT2900.10213029712872995No Hit
TGCTACAGTAGACTAAGAAGCAGCAAGCAAGTGCTTCCACTG2860.10072160337523024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATAC309.374198E-750.7805321
GTTACCC204.1366648E-450.0933923
ACCAAAT457.4251147E-944.52746224
AAAACCA501.6689228E-840.6244221
TCATACT453.348614E-739.5065922
CCCTATT406.785709E-638.08539620
GAAAACC553.8666258E-836.9312920
ACGTTTG554.3599357E-836.43155724
CCAAATA554.3599357E-836.43155725
CTCCATG507.660783E-735.54636819
CGACCCT508.523366E-735.06537625
TGCCCCA200.002500057734.7573621
CGCTATA301.2723438E-534.7573621
CGCTACC406.6136636E-834.7573621
CAGTGCG2700.034.7126356
CAGGGTC700.034.712636
CAGGCGC2050.034.712636
CAGCTAT454.8585207E-934.712636
CAGTAGG200.002515903734.712636
CCGCACT200.002515903734.712636
Produced by FastQC (version 0.11.5)