FastQCFastQC Report
Thu 26 May 2016
SRR2099063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309638
Sequences flagged as poor quality0
Sequence length42
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTTGCCAAGGAAGGAAGGGAGGATGGAAGGAAGGAA23200.7492620414806969No Hit
GACTACGTTGCCAAGGAAGGAAGGNAGGATGGAAGGAAGGAA15060.48637441140945237No Hit
GACTACGTTATTCCCAGCCCACTCAACCAACCCACCAGTCCT5060.16341663490915198No Hit
GACTACGGGGTAGCCGATCAGCGACTCTTCGATGAGCAGCTC4840.1563115638261454No Hit
GACTACGTATCGCTGCTGCAGGGCAGCATCGAGAATATGCAG4370.14113254833063124No Hit
GGCGACGTTCGATCCGACGATCTCGATAATAGCGCCAGTGAC3860.12466170172911593No Hit
GACTACGGGGTAGCCGATCAGCGANTCTTCGATGAGCAGCTC3780.12207803951711353No Hit
GACTACGTGAGAAAAATGGTAAGTATGTGAGATGATGGATTT3740.12078620841111233No Hit
GACTACGTTAAGCAGTCCGACGATCGAAAGACTACGTAGTCA3730.12046325063461204No Hit
GACTACGTCGTTCAGTCCGACGATCCGGCGATCGTCGGACTG3570.11529592621060722No Hit
GACTACGTGCGATTCGACGATCGTCGGACTGTAGAACTCTGA3440.11109747511610331Illumina DpnII expression Sequencing Primer (100% over 24bp)
GACTACGTCAACGGAAAGTTCAACATCAGACAGTCCAACCTC3310.10689902402159941No Hit
GACTACGTGGACTTCTACAGTCCGACGATCGTAGGACTGTAG3260.1052842351390979No Hit
TGCTACGTCAGTTCCGACGATCCAGTAGAAAAACAAAAAAAA3100.1001169107150931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTAG150.00792033850.7814621
GCCCTGA150.00792033850.7814621
AAGACTC203.86806E-450.78145621
CAACATC850.047.79431521
TCTTTGA600.046.5496722
GGTGTAC401.4778561E-743.84840823
TCGATAA651.8189894E-1242.40285523
CGCCTGA604.1836756E-1142.31788322
ATCCGCA900.041.7603923
GCCTGAC604.9112714E-1141.7603923
ATCGGTA604.9112714E-1141.7603924
ACATCAG1050.040.56723423
CTCGCAA250.001239585740.08997325
GCTCGCA250.001239585740.08997324
CTCGATA705.456968E-1239.89971522
AACATCA1050.038.69063622
TCCAGTA406.7878173E-638.08609421
TCCGCAG1000.037.5843524
CCGCAGA1000.037.5843525
TTCAACA1100.036.93197319
Produced by FastQC (version 0.11.5)